Optimization of the production of Exopolysaccharide (EPS) from biofilm-forming bacterial consortium using different parameters

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100117 - 100117

Published: July 4, 2024

Bacterial cells produce a variety of exopolysaccharide (EPS) throughout their life cycle. EPS plays crucial role in the formation biofilms and control environmental processes microbial communities. The bacterial community benefits from several ways, including nutrient compartmentation storage, stress resistance synergism. generation by consortium-associated bacteria, which involves microorganisms, is versatile efficient for use industrial processes, such as biotechnology pollution degradation. qualitative quantitative chemical characteristics consortium's are mostly determined type culture utilized, type, temperature, pH. main goal study was to find out how biosynthesis works consortium culture. To achieve maximum regeneration, pH, carbon, nitrogen sources were optimized. A three strains i.e. Klebsiella pneumonia strain, Pseudomonas aeruginosa strain Burkholderia cepacian that distinguished capacity developed part study. total production detected after 8 days incubation at pH 7.5 (15.5 g/L) temperature 35 °C (12.60 g/L). optimum identified 17.84 g/L 21.07 when ammonium sulfate glucose employed carbon supplements, respectively. protein concentration 3067 µg/mL, more than carbohydrate (263.10 µg/mL) DNA (7.60 content. protein/carbohydrate ratio present 11.65, much higher prior investigations. FTIR analysis confirmed functional groups carbohydrates, proteins, lipids DNA, viz., CO, COOH, NH, OH, CH OCH3. study's findings revealed enhancing molecules lowering biofilm-related infections improving efficacy antimicrobial treatments wastewater.

Language: Английский

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Biofilm Inhibition on Medical Devices and Implants Using Carbon Dots: An Updated Review

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(5), P. 2604 - 2619

Published: April 16, 2024

Biofilms are an intricate community of microbes that colonize solid surfaces, communicating via a quorum-sensing mechanism. These microbial aggregates secrete exopolysaccharides facilitating adhesion and conferring resistance to drugs antimicrobial agents. The escalating global concern over biofilm-related infections on medical devices underscores the severe threat human health. Carbon dots (CDs) have emerged as promising substrate combat disrupt biofilm matrices. Their numerous advantages such facile surface functionalization specific properties, position them innovative anti-biofilm Due their minuscule size, CDs can penetrate cells, inhibiting growth cytoplasmic leakage, reactive oxygen species (ROS) generation, genetic material fragmentation. Research has demonstrated efficacy in biofilms formed by key pathogenic bacteria

Language: Английский

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Development of predictive models of biofilm formation by C. sakazakii, E. cloacae on surfaces used in the food industry and medicine

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 434, P. 111131 - 111131

Published: Feb. 24, 2025

Language: Английский

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Bacteriophages with depolymerase activity in the control of antibiotic resistant Klebsiella pneumoniae biofilms

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 13, 2023

Klebsiella pneumoniae is associated with a variety of infections, such as pneumonia, urogenital infection, liver abscess, and bloodstream infection. It especially dangerous for patients in medical facilities, where it can cause ventilator-associated pneumonia or intensive care unit-acquired pneumonia. The emergence multidrug-resistant hypervirulent strains well the ability to form biofilms on various devices complicates treatment infections makes use antibiotics ineffective. application bacteriophages promising alternative combating biofilms. In present study cocktail 3 depolymerase activity was used control antibiotic resistant vitro. Biofilms were examined using optical scanning electron microscopy. obtained results demonstrate that studied bacteriophage effectively disrupt

Language: Английский

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Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects

Microorganisms, Journal Year: 2023, Volume and Issue: 11(3), P. 704 - 704

Published: March 9, 2023

Hypervirulent Klebsiella pneumoniae (hvKp) is emerging worldwide. Hypermucoviscousity the characteristic trait that distinguishes it from classic K. (cKp), which enables Kp to cause severe invasive infections. This research aimed investigate hypermucoviscous (hmvKp) phenotype among gut commensal isolated healthy individuals and attempted characterize genes encoding virulence factors may regulate hypermucoviscosity trait. Using string test, 50 identified isolates individuals’ stool samples were examined for investigated by transmission electron microscopy (TEM). Antimicrobial susceptibility profiles of determined using Kirby Bauer disc method. tested different PCR. Biofilm formation was assayed microtiter plate All multidrug-resistant (MDR). Phenotypically, 42% hmvKp. PCR-based genotypic testing revealed hmvKp belonged capsular serotype K2. study harbored more than one gene. The magA rmpA not detected, while terW gene present in all isolates. siderophores entB irp2 most prevalent (90.5%) non-hmvKp (96.6%), respectively. wabG uge with rates 90.5% 85.7%, outcomes this highlight potential health risk diseases, owing being MDR, harboring multiple genes. absence essential related such as phenotypes suggests multifactorial complexity or hypervirulence traits. Thus, further studies are warranted verify hypermucoviscosity-related pathogenic colonization niches.

Language: Английский

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Evaluation of the Ability to Form Biofilms in KPC-Producing and ESBL-Producing Klebsiella pneumoniae Isolated from Clinical Samples

Antibiotics, Journal Year: 2023, Volume and Issue: 12(7), P. 1143 - 1143

Published: July 2, 2023

The appearance of Klebsiella pneumoniae strains producing extended-spectrum β-lactamase (ESBL), and carbapenemase (KPC) has turned into a significant public health issue. ESBL- KPC-producing K. pneumoniae's ability to form biofilms is concern as it can promote the spread antibiotic resistance prolong infections in healthcare facilities. A total 45 were isolated from human infections. Antibiograms performed for 17 antibiotics, ESBL production was tested by Etest PM/PML, rapid test used detect KPC carbapenemases, genes detected PCR. Biofilm microtiter plate method. 73% multidrug found, with highest rates ampicillin, trimethoprim-sulfamethoxazole, cefotaxime, amoxicillin-clavulanic acid, aztreonam. Simultaneously, most effective antibiotics tetracycline amikacin. blaCTX-M, blaTEM, blaSHV, aac(3)-II, aadA1, tetA, cmlA, catA, gyrA, gyrB, parC, sul1, sul2, sul3, blaKPC, blaOXA, blaPER detected. showed that 80% biofilm producers. Most isolates weak producers (40.0% 60.0%, respectively). There no correlation between stronger presence enzymes isolates.

Language: Английский

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Potential of an Isolated Bacteriophage to Inactivate Klebsiella pneumoniae: Preliminary Studies to Control Urinary Tract Infections

Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 195 - 195

Published: Feb. 19, 2024

Urinary tract infections (UTIs) caused by resistant Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages. In the present study, we isolated phage VB_KPM_KP1LMA (KP1LMA) from sewage water a K. strain as host. Whole-genome analysis indicated that genome was double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content did not contain virulence or antibiotic resistance genes. The inactivation potential of KP1LMA assessed in broth at an MOI 1 10, maximum 4.9 5.4 log CFU/mL, respectively, observed after 9 h. efficacy 10 also urine evaluate phage’s performance acidic environment. A 3.8 CFU/mL results suggest could potentially control UTI this pneumoniae, indicating same procedure be used UTIs other strains if new specific phages are isolated. Although has narrow host range, future, efforts made expand its spectrum activity combine others, enabling use against involved UTIs.

Language: Английский

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Combating bacterial biofilms and related drug resistance: Role of phyto-derived adjuvant and nanomaterials

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 195, P. 106874 - 106874

Published: Aug. 23, 2024

Language: Английский

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Potential of a New Bacteriophage to Control <em>Klebsiella pneumoniae</em>: Preliminary Studies to Control Urinary Tract Infections

Published: Jan. 8, 2024

Urinary tract infections (UTIs) caused by Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages (phages). In the present study, we isolated phage KP-1 from sewage water K. as a host. Whole genome analysis indicated that was double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content did not contain virulence or antibiotic resistance genes. The inactivation potential of assessed in broth at an MOI 1 10, maximum 4.9 5.4 log CFU/mL observed after 9 h, respectively. 10 efficacy also urine evaluate phage’s performance acidic environment. A 3.8 h. results indicate could be used control UTIs pneumoniae; however, further studies cocktails broader spectrum activity phages micro- nanocarriers avoid negative effect low pH on viability, are necessary.

Language: Английский

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Comparative analysis of multidrug-resistant Klebsiella pneumoniae strains of food and human origin reveals overlapping populations

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 413, P. 110605 - 110605

Published: Jan. 23, 2024

Given the increasing incidence of multidrug-resistant (MDR) Klebsiella pneumoniae infections, it is great interest to investigate risk transmission associated with prevalence this pathogen. Some studies have described fresh raw poultry meat as a reservoir MDR K. pneumoniae, including clinically relevant sequence types (ST) and extended-spectrum β-lactamase (ESBL) strains, indicating possible consumer exposure. This study compared 47 strains from human clinical isolates assess similarities, analysis antimicrobial resistance profiles virulence factors involved in infection. In addition, several biofilm culture methods were evaluated for reproducible assessment formation strains. Globally, no association between strain origin STs, hypermucoviscosity, or serum could be found food origin, nor an AMR pattern, suggesting overlapping populations. We that LB supplemented glucose microaerobiosis was best discrimination condition active attachment cultivation model. The capacity strongly dependent on conditions, strain-specific response, but only minor increase levels recorded Our results suggest similar zoonosis potentially virulent foodborne previously observed E. coli also present high-priority further confirms pose consumers therefore pathogen should included surveillance pathogens high infections therapeutic failure.

Language: Английский

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