Screening of mitochondrial-related biomarkers connected with immune infiltration for acute respiratory distress syndrome through WGCNA and machine learning DOI Creative Commons
Wei Sun, Su Tu

Medicine, Год журнала: 2025, Номер 104(10), С. e41497 - e41497

Опубликована: Март 7, 2025

Septic acute respiratory distress syndrome (ARDS) is a complex and noteworthy type, but its molecular mechanism has not been fully elucidated. The aim to explore specific biomarkers diagnose sepsis-induced ARDS. Gene expression data of sepsis alone ARDS were downloaded from public databases, the differential immune cells expressed genes between 2 groups screened. Weighted gene co-expression network analysis was used identify cells-related module genes, then integrated with mitochondrial obtain common genes. Next, least absolute shrinkage selection operator, random forest, support vector machine-recursive feature elimination utilized construct nomogram model. Meanwhile, biological function targeted drugs analyzed. abundance 3 (macrophage, neutrophils, monocytes) significantly different groups. machine learning identified 5 up-regulated in had diagnostic significance. based on these good confidence clinical application value. set enrichment showed that phenylalanine metabolism pathway increased samples positive correlation Drug prediction exhibited chlorzoxazone, ajmaline, clindamycin could target multiple Overall, signature screened this study can effectively predict possibility patients, which deepen understanding pathogenesis therapy development.

Язык: Английский

Roles of Macrophages and Endothelial Cells and Their Crosstalk in Acute Lung Injury DOI Creative Commons
Sara Osorio Valencia, Bisheng Zhou

Biomedicines, Год журнала: 2024, Номер 12(3), С. 632 - 632

Опубликована: Март 13, 2024

Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), present life-threatening conditions characterized by inflammation endothelial injury, leading to increased vascular permeability edema. Key players in the pathogenesis resolution of ALI are macrophages (Mφs) cells (ECs). The crosstalk between these two cell types has emerged as a significant focus for potential therapeutic interventions ALI. This review provides brief overview roles Mφs ECs their interplay ALI/ARDS. Moreover, it highlights significance investigating perivascular (PVMs) immunomodulatory (IMECs) crucial participants Mφ–EC crosstalk. sheds light on paves way innovative treatment approaches.

Язык: Английский

Процитировано

11
Extracellular vesicles from Candida albicans modulate immune cells function and play a protective role in fungal keratitis DOI

Huijin Duan,

Fanyue Meng,

Xing Liu

и другие.

Microbial Pathogenesis, Год журнала: 2024, Номер 189, С. 106606 - 106606

Опубликована: Март 2, 2024

Язык: Английский

Процитировано

7
Th17/Treg balance: the bloom and wane in the pathophysiology of sepsis DOI Creative Commons
Xinyong Liu, Longwang Chen, Wei Peng

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 15, 2024

Sepsis is a multi-organ dysfunction characterized by an unregulated host response to infection. It associated with high morbidity, rapid disease progression, and mortality. Current therapies mainly focus on symptomatic treatment, such as blood volume supplementation antibiotic use, but their effectiveness limited. Th17/Treg balance, based its inflammatory property, plays crucial role in determining the direction of regression organ damage sepsis patients. This review provides summary changes T-helper (Th) 17 cell regulatory T (Treg) differentiation function during sepsis, heterogeneity balance response, relationship between damage. exerts significant control over bloom wanes throughout sepsis.

Язык: Английский

Процитировано

7
Inhalable and bioactive lipid-nanomedicine based on bergapten for targeted acute lung injury therapy via orchestrating macrophage polarization DOI
Ran Liao, Zhichao Sun, Liying Wang

и другие.

Bioactive Materials, Год журнала: 2024, Номер 43, С. 406 - 422

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

5
Sinensetin, a polymethoxyflavone from citrus fruits, ameliorates LPS-induced acute lung injury by suppressing Txnip/NLRP3/Caspase-1/GSDMD signaling-mediated inflammatory responses and pyroptosis DOI

Zaibin Xu,

Hui-Yu Hu,

Kongyan Wang

и другие.

Food & Function, Год журнала: 2024, Номер 15(14), С. 7592 - 7604

Опубликована: Янв. 1, 2024

The functional effect and molecular mechanism diagram of SIN ameliorated ALI.

Язык: Английский

Процитировано

4
Extracellular Vesicle ASC: A Novel Mediator for Lung–Brain Axis in Preterm Brain Injury DOI

N. Starke,

Naga Venkata Divya Challa,

Huijun Yuan

и другие.

American Journal of Respiratory Cell and Molecular Biology, Год журнала: 2024, Номер 71(4), С. 464 - 480

Опубликована: Июль 3, 2024

Bronchopulmonary dysplasia (BPD) and neurodevelopmental impairment are among the most common morbidities affecting preterm infants. Although BPD is a predictor of poor outcomes, it currently uncertain how contributes to brain injury in Extracellular vesicles (EVs) involved interorgan communication diverse pathological processes. ASC (apoptosis-associated speck-like protein containing caspase recruitment domain) pivotal inflammasome assembly activation inflammatory response. We assessed expression profiles alveolar macrophage (AM) markers CD11b, CD11c, CD206 as well EVs isolated from plasma infants at risk for 1 week age. found that on higher fraction inspired oxygen therapy (HO

Язык: Английский

Процитировано

4
Biomimetic Nanomodulator Regulates Oxidative and Inflammatory Stresses to Treat Sepsis-Associated Encephalopathy DOI

Haijing Qu,

Jie Wu, Yuqing Pan

и другие.

ACS Nano, Год журнала: 2024, Номер 18(41), С. 28228 - 28245

Опубликована: Окт. 5, 2024

Sepsis-associated encephalopathy (SAE) is a devastating complication of sepsis, affecting approximately 70% patients with sepsis in intensive care units (ICU). Although the pathophysiological mechanisms remain elusive, typically accompanied by systemic inflammatory response syndrome (SIRS) and hyper-oxidative conditions. Here, we introduce biomimetic nanomodulator (mAOI NP) that specifically targets inflammation site simultaneously regulates oxidative stresses. mAOI NPs are constructed using metal-coordinated polyphenolic antioxidants (tannic acid) flavonoid quercetin, which then coated macrophage membrane to enhance pharmacokinetics enable SAE targeting. In cecal ligation puncture (CLP)-induced severe model, effectively mitigate stress purging reactive oxygen species, repairing mitochondrial damage activating Nrf2/HO-1 signaling pathway; while polarizing M1 macrophages or microglia toward anti-inflammatory M2 subtype. potently inhibit progress, prolong overall survival from 25 66% learning memory capabilities mice. Further proteomics analysis reveals modulate neurodevelopment processes related formation also exerting antioxidative effects on brain tissue responses associated pathology. This study offers significant potential for improving patient outcomes revolutionizing treatment landscape this sepsis.

Язык: Английский

Процитировано

4
Macrophages in sepsis-induced acute lung injury: exosomal modulation and therapeutic potential DOI Creative Commons

Kangzheng Lv,

Qun Liang

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Sepsis-induced acute lung injury (ALI) remains a leading cause of mortality in critically ill patients. Macrophages, key modulators immune responses, play dual role both promoting and resolving inflammation. Exosomes, small extracellular vesicles released by various cells, carry bioactive molecules that influence macrophage polarization responses. Emerging researchers have identified exosomes as crucial mediators modulate activity during sepsis-induced ALI. This review explores the modulating functions, focusing on cellular interactions within microenvironment their potential therapeutic targets. It highlights regulation macrophages derived from pathogenic germs, neutrophils, alveolar epithelial mesenchymal stromal cells. By understanding these mechanisms, it aims to uncover innovative strategies for

Язык: Английский

Процитировано

0
Mogroside V Reverse Macrophage Polarization to Alleviate Cytokine Storm-Induced Acute Lung Injury Through Tlr4/Nf-Κb Pathway DOI
Yue Zhao,

Jiacai Wu,

Yang Yang

и другие.

Опубликована: Янв. 1, 2025

Background: Cytokine storm (CS) represents a critical systemic inflammatory syndrome characterized by heightened concentrations of circulating cytokines and hyperactivation immune cells. Acute lung injury (ALI) serves as significant prognostic indicator for mortality in patients experiencing CS. Mogroside V (Notas et al.), the principal bioactive compound derived from Siraitia grosvenorii traditional Chinese medicine, exhibits potential anti-inflammatory anti-tumor properties. However, specific role MV modulating response during ALI treatment remains inadequately elucidated.Purpose: To investigate mechanisms vitro vivo against induced CS.Methods: We constructed an CS model M1-like macrophage mode. Through analysis data Gene Expression Omnibus (GEO) database, combined with immunohistochemistry, immunofluorescence, flow cytometry, Western blotting, ELISA, cell heat shift assay, molecular docking experiments, mechanism alleviating caused was researched.Results: Transcriptomic revealed that CS-induced is associated TLR4/NF-κB pathway polarization. In experiments demonstrated significantly improved survival rate mice, promoted resolution neutrophils, mitigated histopathological damage. Both collectively inhibited expression TLR4, MyD88, P-IκBα P-p65 pathway, blocked nuclear translocation p65, consequently suppressed its transcriptional activity, leading to reduced production (TNF-α, IL-6, NO). Immunohistochemistry cytometry analyses reduction F4/80 CD86 expression, while CD206, IL-4, IL-10 were elevated, indicating reduces total population promotes polarization macrophages toward M2 phenotype.Conclusion: This research indicates demonstrates therapeutic effect on ALI, potentially through inhibition signaling modulation Given low adverse effects high bioactivity, emerges promising candidate further investigation.

Язык: Английский

Процитировано

0
Efficacy of caerulomycin A in modulating macrophage polarization and cytokine response in a murine model of lipopolysaccharide-induced sepsis DOI Creative Commons
Jun Zhang,

Shiyue Tang

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Янв. 10, 2025

Sepsis is characterized by an excessive immune response. Modulation of the response, particularly macrophage polarization, may provide therapeutic benefit. The effects Caerulomycin A (caeA), a known STAT1 phosphorylation inhibitor, on polarization and inflammatory markers were explored using lipopolysaccharide (LPS)-induced sepsis mouse model. model was established in C57BL/6 mice induced intraperitoneal injection LPS, survival rate observed after treatment with different doses caeA to determine optimal dose. For in-vitro assays RAW264.7 cell line, concentration that non-toxic screened MTT assay, followed analyses qRT-PCR, ELISA, Western blot flow cytometry for M1/M2 type (CD86, NOS2, CD206, ARG1) factors (IL-1β, IL-6, TNF-α, IL-4, IL-10) expression. In addition, levels STAT6 JAK–STAT signaling pathway detected. results in-vivo experiments showed (20 mg/kg) significantly increased LPS-induced septic decreased expression M1-type (CD86 NOS2) pro-inflammatory cytokines TNF-α) while increasing M2-type (CD206 anti-inflammatory (IL-4 experiments, 20 μM effectively inhibited macrophages without affecting activity cells, yet enhanced level STAT6, as detected blot. CaeA modulates attenuates response mice, possibly pathway. These findings support further exploration potential agent sepsis.

Язык: Английский

Процитировано

0