bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 8, 2023
Abstract
Recent
and
remote
memories
are
encoded
throughout
the
brain
in
‘Engrams’:
cell
ensembles
formed
during
acquisition,
upon
their
reactivation,
a
specific
memory
can
be
recalled.
The
maturation
of
engrams
from
recent
to
time
points
involves
recruitment
CA1
neurons
projecting
anterior
cingulate
cortex
(CA1→ACC).
Various
modifications
astrocytes,
Gq-
or
Gi-GPCR
pathways,
acquisition
were
shown
affect
recall
seemingly
contradictory
ways.
To
address
inconsistency,
we
applied
transgenic
approaches
for
ensemble
identification,
CLARITY,
retro-AAV
virus
circuit
mapping,
chemogenetics
astrocytes
functional
investigation.
We
manipulated
activity
either
Gi-pathways
tagged
cFos+
engram
cells
CA1→ACC
same
animals.
behavioral
results
coupled
with
changes
projection
engram,
demonstrated
by
number
as
well
axons
toward
ACC:
Gq
pathway
activation
caused
enhancement
alone
was
accompanied
earlier
engram.
In
contrast,
when
activating
Gi
only
impaired,
not
recruited
memory.
Finally,
provide
simple
working
model,
hypothesizing
that
control
performance
targeting
projection.
Specifically,
Gi-pathway
differently,
but
do
so
modulating
mechanism.
These
findings
illuminate
importance
fear
implications
on
recall.
Cell Reports,
Год журнала:
2024,
Номер
43(3), С. 113943 - 113943
Опубликована: Март 1, 2024
The
maturation
of
engrams
from
recent
to
remote
time
points
involves
the
recruitment
CA1
neurons
projecting
anterior
cingulate
cortex
(CA1→ACC).
Modifications
G-protein-coupled
receptor
pathways
in
astrocytes
affect
and
recall
seemingly
contradictory
ways.
To
address
this
inconsistency,
we
manipulated
these
during
memory
acquisition
tagged
c-Fos-positive
engram
cells
CA1→ACC
recall.
behavioral
results
were
coupled
with
changes
projection
engram:
Gq
pathway
activation
caused
enhancement
alone
was
accompanied
by
earlier
engram.
In
contrast,
Gi
resulted
impairment
only
recall,
not
recruited
memory.
Finally,
provide
a
simple
working
model,
hypothesizing
that
differently,
modulating
same
mechanism:
projection.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2774 - 2774
Опубликована: Март 19, 2025
Anxiety
is
a
physiological,
emotional
response
that
anticipates
distal
threats.
When
kept
under
control,
anxiety
beneficial
response,
helping
animals
to
maintain
heightened
attention
in
environments
with
potential
dangers.
However,
an
overestimation
of
threats
can
lead
excessive
expression
that,
humans,
may
evolve
into
disorders.
Pharmacological
treatments
show
variable
efficacy
among
patients,
highlighting
the
need
for
more
efforts
better
understand
pathogenesis
Mounting
evidence
suggests
astrocytes,
type
glial
cells,
are
active
partners
neurons
brain
circuits
and
regulation
behaviors
both
physiological
pathological
conditions.
In
this
review,
I
summarize
current
literature
on
role
astrocytes
from
different
regions
modulating
anxious
states,
goal
exploring
novel
cerebral
mechanisms
identify
innovative
therapeutic
targets
treatment
The Journal of Physiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 4, 2025
In
the
brain,
astrocytes
express
glutamate
receptors
to
detect
glutamatergic
signalling
at
synapses.
Numerous
studies
have
been
conducted
characterize
types
and
levels
of
receptor
expression,
their
physiological
properties
functional
roles.
The
metabotropic
5
(mGluR5)
is
particularly
noteworthy.
Although
it
described
as
only
classical
excitatory
expressed
by
astrocytes,
recent
indicate
that
its
expression
are
lower
in
adulthood
compared
postnatal
period.
Nevertheless,
mGluR5
activation
remains
sufficient
induce
robust
astrocytic
activity.
To
resolve
these
seemingly
contradictory
findings,
we
review
current
understanding
physiology
mGluR5,
level
variations
throughout
life,
consequences
summary,
maintain
which
triggers
Ca2+
This
intracellular
processes
modulate
synaptic
circuit
function,
relevant
due
extensive
brain.
Moreover,
most
on
using
cultured
or
acute
brain
slices
from
young
rodents.
Therefore,
further
research
needed
investigate
roles
adulthood,
not
function
but
also
behavioural
processing.
Understanding
regulation
levels,
during
crucial
for
fully
circuits
role
pathophysiology
various
neurobiological
diseases.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(1), С. 581 - 581
Опубликована: Янв. 1, 2024
Astrocytes
are
pivotal
for
synaptic
transmission
and
may
also
play
a
role
in
the
induction
expression
of
plasticity,
including
endocannabinoid-mediated
long-term
depression
(eCB-LTD).
In
dorsolateral
striatum
(DLS),
eCB
signaling
plays
major
balancing
excitation
inhibition
promoting
habitual
learning.
The
aim
this
study
was
to
outline
astrocytes
regulating
DLS.
To
end,
we
employed
electrophysiological
slice
recordings
combined
with
metabolic,
chemogenetic
pharmacological
approaches
an
attempt
selectively
suppress
astrocyte
function.
High-frequency
stimulation
induced
eCB-mediated
LTD
(HFS-LTD)
brain
slices
from
both
male
female
rats.
metabolic
uncoupler
fluorocitrate
(FC)
reduced
probability
transmitter
release
depressed
output
manner
that
independent
on
cannabinoid
1
receptor
(CB1R)
activation.
Fluorocitrate
did
not
affect
by
CB1R
agonist
WIN55,212-2,
but
enhanced
CB1R-dependent
HFS-LTD.
Reduced
neurotransmission
facilitated
HFS-LTD
were
observed
during
manipulation
using
Gi-coupled
DREADDs
targeting
glial
fibrillary
acidic
protein
(GFAP)-expressing
cells,
connexins
carbenoxolone
disodium,
or
astrocytic
glutamate
uptake
TFB-TBOA.
While
pretreatment
N-methyl-D-aspartate
(NMDA)
antagonist
2-amino-5-phosphonopentanoic
acid
(APV)
failed
prevent
FC,
it
blocked
facilitation
lack
tools
disentangle
neurons
is
limitation
study,
our
data
collectively
support
modulating
basal
plasticity.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 23, 2024
How
astrocytes
regulate
neuronal
circuits
is
a
fundamental,
unsolved
question
in
neurobiology.
Nevertheless,
few
studies
have
explored
the
rules
that
govern
when
respond
to
different
neurotransmitters
Current Neuropharmacology,
Год журнала:
2024,
Номер
22(13), С. 2217 - 2239
Опубликована: Янв. 29, 2024
:
Memory
refers
to
the
imprint
accumulated
in
brain
by
life
experiences
and
represents
basis
for
humans
engage
advanced
psychological
activities
such
as
thinking
imagination.
Previously,
research
focused
on
memory
have
always
targeted
neurons.
However,
addition
neurons,
astrocytes
are
also
involved
encoding,
consolidation,
extinction
of
memory.
In
particular,
known
affect
recruitment
function
neurons
at
level
local
synapses
networks.
Moreover,
involvement
memory-related
disorders,
especially
Alzheimer’s
disease
(AD)
post-traumatic
stress
disorder
(PTSD),
has
been
investigated
extensively.
this
review,
we
describe
unique
contributions
synaptic
plasticity
neuronal
networks
discuss
role
different
types
processing.
addition,
explore
roles
pathogenesis
AD,
aging,
PTSD
addiction,
thus
suggesting
that
targeting
may
represent
a
potential
strategy
treat
neurological
diseases.
conclusion,
review
emphasizes
from
perspective
will
provide
new
ideas
diagnosis
therapy
disorders.
eNeuro,
Год журнала:
2024,
Номер
11(6), С. ENEURO.0056 - 24.2024
Опубликована: Июнь 1, 2024
Formation
and
retrieval
of
remote
contextual
memory
depends
on
cortical
engram
neurons
that
are
defined
during
learning.
Manipulation
astrocytic
G
q
i
associated
G-protein
coupled
receptor
(GPCR)
signaling
has
been
shown
to
affect
processing,
but
little
is
known
about
the
role
s
-GPCR
in
acquisition
functioning
neurons.
We
assessed
this
by
chemogenetic
manipulation
astrocytes
medial
prefrontal
cortex
(mPFC)
male
mice,
either
encoding
or
consolidation
a
fear
memory,
while
simultaneously
labeling
found
stimulation
did
not
alter
expression.
In
line
with
this,
size
mPFC
population
recall-induced
reactivation
these
was
unaffected.
Hence,
our
data
indicate
activation
sufficient
performance
