Advances in experimental medicine and biology, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Journal of Experimental Biology, Год журнала: 2023, Номер 226(4)
Опубликована: Фев. 15, 2023
ABSTRACT Diapause, a stage-specific developmental arrest, is widely exploited by insects to bridge unfavorable seasons. Considerable progress has been made in understanding the ecology, physiology and evolutionary implications of insect diapause, yet intriguing questions remain. A more complete diapause processes on Earth requires better geographic spread investigations, including work tropics at high latitudes. Questions surrounding energy management trade-offs between non-diapause remain understudied. We know little about how maternal effects direct response, regulators prolonged are also poorly understood. Numerous factors that were recently linked still waiting be placed regulatory network leading from photoreception engagement program. These include epigenetic small noncoding RNAs, emerging data suggest role for microbiome regulation. Another feature complexity resulting diverse suite responses comprise syndrome. Select transcription likely serve as master switches turning these responses, but we far full complexity. The richness species displaying offers platform seeking common components ‘diapause toolbox’. Across latitudes, during invasion events changing climate, grand opportunities probe change speciation. At practical level, can manipulated control long-term storage. Diapausing contain treasure trove pharmacological compounds offer promising models human health.
Язык: Английский
Процитировано
51
Biochemical Society Transactions, Год журнала: 2023, Номер 51(5), С. 1847 - 1856
Опубликована: Окт. 6, 2023
Diapause is a protective mechanism that many organisms deploy to overcome environmental adversities. extends lifespan and fertility enhance the reproductive success survival of species. Although diapause states have been known employed for commercial purposes, example in silk industry, detailed molecular cell biological studies are an exciting frontier. Understanding diapause-like mechanisms will shed light on pathways steer through adverse conditions. One hope understanding support might be leveraged extend and/or health span humans as well species threatened by climate change. In addition, recent findings suggest cancer cells persist after treatment mimic states, implying these programs may facilitate from chemotherapy cause relapse. Here, we review underlying variety organisms, discuss supporting tumor persister cells.
Язык: Английский
Процитировано
29
Development, Год журнала: 2023, Номер 150(10)
Опубликована: Май 15, 2023
ABSTRACT Female insects can enter reproductive diapause, a state of suspended egg development, to conserve energy under adverse environments. In many insects, including the fruit fly, Drosophila melanogaster, also frequently called dormancy, is induced low-temperature and short-day conditions by downregulation juvenile hormone (JH) biosynthesis in corpus allatum (CA). this study, we demonstrate that neuropeptide Diuretic 31 (DH31) produced brain neurons project into CA plays an essential role regulating dormancy suppressing JH adult D. melanogaster. The expresses gene encoding DH31 receptor, which required for DH31-triggered elevation intracellular cAMP CA. Knocking down Dh31 these CA-projecting or receptor suppresses decrease titer, normally observed dormancy-inducing conditions, leading abnormal yolk accumulation ovaries. Our findings provide first molecular genetic evidence demonstrating peptidergic play biosynthesis.
Язык: Английский
Процитировано
25
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(27)
Опубликована: Июнь 25, 2024
To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While flies are less active during diapause, it is unclear how environmental conditions affect circadian rhythms and sleep. Here we show that diapause-inducing temperatures, melanogaster exhibit altered activity profiles, including severely reduced morning an advanced evening peak. Consequently, the have single peak at time similar when nondiapausing take siesta. Temperatures ≤15 °C, rather than photoperiod, primarily drive this behavior. At rapidly deep-sleep lacks sleep cycles of higher require high levels stimulation for arousal. Furthermore, 25 prefer siesta shade, preference virtually eliminated 10 °C. Resting shade driven by aversion blue light sensed Rhodopsin 7 outside eyes. Flies °C neuronal markers elevated pressure, increased expression Bruchpilot Ca 2+ R5 ellipsoid body neurons. Therefore, pressure might overcome aversion. Thus, same cause arrest, preserve germline stem cells, extend lifespan, D. prone deep dramatically altered, yet rhythmic, daily patterns.
Язык: Английский
Процитировано
4
Insects, Год журнала: 2023, Номер 14(7), С. 621 - 621
Опубликована: Июль 10, 2023
Hermetic storage methods are effective at protecting grain against insect pests. Biotic and abiotic factors influence oxygen depletion during hermetic storage. We investigated the dual effects of temperature initial pest infestation level on airtight Glass jars filled with cowpea were infested (25 or 50 adult bruchids), then hermetically sealed stored 20, 30, 40 °C for 30 days. Oxygen depletion, relative humidity, monitored. Germination, moisture content, damage weight loss, emergence assessed. varied by level. However, was optimum (reaching 5% less in 10 days) regardless No changes observed germination minimal damage, loss (<1%). Only 20 insects able to survive after days emerged 45 post-treatment under normoxia. Therefore, containers should remain closed more than minimize re-infestation areas where average ambient temperatures rarely exceed 23 °C. Further research is needed assess effect low survival beyond
Язык: Английский
Процитировано
11
Insects, Год журнала: 2024, Номер 15(2), С. 136 - 136
Опубликована: Фев. 17, 2024
(Okamoto) displays typical adult reproductive diapause under short photoperiods; however, our understanding of the molecular mechanism underlying photoperiod-sensitive reproduction remains limited. In this study, we performed transcriptome profiling four treatments (the diapause-sensitive stage and pre-diapause phase long photoperiods)
Язык: Английский
Процитировано
3
Cell Reports, Год журнала: 2024, Номер 43(11), С. 114939 - 114939
Опубликована: Ноя. 1, 2024
Many insect species, including the economically important pest Helicoverpa armigera, avoid unfavorable conditions by suspending development. This form of phenotypic plasticity-facultative diapause-is a complex trait, though its evolution and intricate genetic architecture remain poorly understood. To investigate how such polygenic trait could be locally adapted, we explore architecture. We map large-effect diapause-associated locus to Z chromosome crossing high- low-latitude populations. By generating multiple chromosome-scale assemblies, identify an ∼5.93-Mb chromosomal inversion that constitutes locus. Within this inversion, 33 genes harbor divergent non-synonymous mutations, notably three circadian rhythm genes: Period, Clock, Cycle. CRISPR-Cas9 knockout experiments confirm each gene is independently essential for pupal diapause. Thus, diapause supergene arose within H. armigera via enabling local climatic adaptation in crop pest.
Язык: Английский
Процитировано
3
Archives of Insect Biochemistry and Physiology, Год журнала: 2025, Номер 118(2)
Опубликована: Фев. 1, 2025
ABSTRACT The primary vector of the West Nile virus, Culex pipiens , undergoes reproductive dormancy during adverse winter season. While our current understanding has mainly focused on cellular signals and phenotypic shifts occurring at a global scale diapause, information tissue‐specific transcriptomic changes remains limited. This knowledge gap is major challenge in interpreting regulatory mechanisms tissue level. To address this, present work utilized RNA‐seq technology to investigate transcriptional head that house brain crucial endocrinal organs such as corpora allata. We obtained RNA samples from heads diapausing nondiapausing female mosquitoes two specific time intervals, ZT0 ZT16, then subjected them sequencing. Our results revealed differences differentially expressed genes between diapause non‐diapause highlighting diel variations gene expression. also selected twelve associated with phenotype examined transcript abundance six different points over 24 h. qRT‐PCR analysis showed similar up‐ downregulation transcripts nondiapause phenotypes thus validating RNA‐seq. In summary, study identified new differentiation their expression, potentially linking photoperiod seasonal insects. newly presented will significantly advance head‐specific for insect diapause.
Язык: Английский
Процитировано
0
Опубликована: Фев. 26, 2025
The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been diapause, 89 were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen selected candidates two Dip-𝛾 Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .
Язык: Английский
Процитировано
0
Frontiers in Physiology, Год журнала: 2025, Номер 16
Опубликована: Март 14, 2025
Insect diapause is a vital survival strategy that enables insects to enter state of suspended development, allowing them withstand unfavorable environmental conditions. During diapause, significantly lower their metabolic rate and build up energy reserves, which they gradually utilize throughout this period. The regulation involves complex interaction hormones enzymes. Juvenile (JHs) affect adults larvae differently; in adults, the absence JH typically triggers while larvae, presence encourages state. Ecdysteroids, regulate molting metamorphosis, are carefully controlled prevent premature development. Reduced signaling insulin-like peptides enhances stress resistance promotes storage. Several enzymes play crucial roles adjustments necessary for diapause. These include degradation JH, ecdysteroidogenic pathway, metabolism fatty acids, glycogen, cryoprotectants, responses. Understanding diapause's molecular biochemical mechanisms essential fundamental entomological research practical applications. Despite recent advances, many aspects regulation, especially interactions among hormonal pathways role enzymes, remain poorly understood. This review analyzes approximately 250 papers consolidate current knowledge on enzymatic It offers comprehensive overview key processes based studies suggests future directions fill gaps our understanding significant biological phenomenon. also lays groundwork enhancing pest control strategies ecological conservation by deepening mechanisms.
Язык: Английский
Процитировано
0