Background
Oral
carcinoma
presents
a
significant
health
challenge,
prompting
the
need
for
innovative
therapeutic
approaches.
Elevation
of
inflammatory
mediators,
including
tumor
necrosis
factor-alpha
(TNF-α)
and
interleukin-6
(IL-6),
has
promoted
cellular
proliferation,
inhibited
apoptosis,
fostered
oral
cancer
progression
through
complex
signaling
pathways.
Hesperidin,
flavanone
glycoside
found
in
citrus
fruits,
is
keen
interest
this
study
as
it
been
proven
to
have
multiple
benefits
vivo
vitro
studies.
However,
mechanism
behind
anticancer
activity
hesperidin
remains
obscure.
Aim
The
aimed
explore
potential
on
human
cells
(KB
cells)
by
modulating
pro-inflammatory
apoptotic
mechanisms.
Methods
Cancer
cell
growth
inhibitory
was
assessed
using
MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium
Bromide)
assay.
Gene
expression
analysis
performed
real-time
RT-PCR
analysis.
In
addition,
silico
docking
conducted
confirm
binding
affinity
with
apoptosis
molecules.
data
were
analyzed
one-way
ANOVA
"t"
test.
Results
Utilizing
assay,
dose-dependent
cytotoxic
effect
unveiled,
remarkable
IC50
value
indicative
its
potent
inhibition
proliferation.
Complementing
these
findings
(p<0.05),
qRT-PCR
demonstrated
hesperidin's
regulatory
influence
key
molecular
targets
within
KB
line.
Hesperidin
treatment
resulted
noteworthy
reduction
TNF-α,
interleukin-1
beta
(IL-1-β),
IL-6,
nuclear
factor
kappa-light-chain-enhancer
activated
B
(NF-κB),
B-cell
lymphoma
2
(Bcl-2)
mRNA
levels
highlighting
role
migration,
inflammation
processes.
Simultaneously,
BAX
indicating
an
enhancement
death.
Molecular
simulations
further
revealed
robust
affinities
between
target
proteins,
suggesting
disrupt
functions
pathways
cells.
Conclusion
effects
line
anti-inflammatory
properties
position
compelling
candidate
exploration
quest
effective
treatments.
These
shed
light
intricate
mechanisms
underlying
promise
agent
against
carcinoma.
Frontiers in Endocrinology,
Год журнала:
2023,
Номер
14
Опубликована: Июнь 13, 2023
Type
II
diabetes
mellitus
(T2DM)
is
a
metabolic
disorder
that
poses
serious
health
concern
worldwide
due
to
its
rising
prevalence.
Hypertension
(HT)
frequent
comorbidity
of
T2DM,
with
the
co-occurrence
both
conditions
increasing
risk
diabetes-associated
complications.
Inflammation
and
oxidative
stress
(OS)
have
been
identified
as
leading
factors
in
development
progression
T2DM
HT.
However,
OS
inflammation
processes
associated
these
two
comorbidities
are
not
fully
understood.
This
study
aimed
explore
changes
levels
plasma
urinary
inflammatory
biomarkers,
along
mitochondrial
biomarkers
connected
dysfunction
(MitD).
These
markers
may
provide
more
comprehensive
perspective
disease
from
no
diabetes,
prediabetes,
coexisting
HT
cohort
patients
attending
clinic
Australia.
Three-hundred
eighty-four
participants
were
divided
into
four
groups
according
status:
210
healthy
controls,
55
prediabetic
patients,
32
87
(T2DM+HT).
Kruskal-Wallis
χ2
tests
conducted
between
detect
significant
differences
for
numerical
categorical
variables,
respectively.
For
transition
prediabetes
interleukin-10
(IL-10),
C-reactive
protein
(CRP),
8-hydroxy-2'-deoxyguanosine
(8-OHdG),
humanin
(HN),
p66Shc
most
discriminatory
generally
displaying
elevated
addition
disrupted
function
revealed
by
HN.
Disease
T2DM+HT
indicated
lower
through
IL-10,
interleukin-6
(IL-6),
interleukin-1β
(IL-1β),
8-OHdG
oxidized
glutathione
(GSSG)
levels,
likely
antihypertensive
medication
use
+HT
patient
group.
The
results
also
better
this
group
shown
higher
HN
which
can
be
attributed
use.
monocyte
chemoattractant
protein-1
(MCP-1)
appeared
independent
medication,
providing
an
effective
biomarker
even
presence
suggest
review
discriminating
stages
or
absence
Our
further
indicate
usefulness
use,
especially
respect
known
involvement
progression,
highlighting
specific
during
therefore
allowing
targeted
individualized
treatment
plan.
Frontiers in Oncology,
Год журнала:
2024,
Номер
14
Опубликована: Июнь 14, 2024
Sirtuins
are
pivotal
in
orchestrating
numerous
cellular
pathways,
critically
influencing
cell
metabolism,
DNA
repair,
aging
processes,
and
oxidative
stress.
In
recent
years,
the
involvement
of
sirtuins
tumor
biology
has
garnered
substantial
attention,
with
a
growing
body
evidence
underscoring
their
regulatory
roles
various
aberrant
processes
within
environments.
This
article
delves
into
sirtuin
family
its
biological
functions,
shedding
light
on
dual
roles—either
as
promoters
or
inhibitors—in
cancers
including
oral,
breast,
hepatocellular,
lung,
gastric
cancers.
It
further
explores
potential
anti-tumor
agents
targeting
sirtuins,
unraveling
complex
interplay
between
miRNAs,
chemotherapeutic
drugs.
The
cancer
reflect
complexity
these
enzymes
but
also
highlight
immense
therapeutic
potential.
These
advancements
hold
significant
promise
for
enhancing
clinical
outcomes,
marking
step
forward
ongoing
battle
against
cancer.
British Journal of Pharmacology,
Год журнала:
2024,
Номер
181(17), С. 3215 - 3231
Опубликована: Май 13, 2024
Nicorandil,
a
selective
opener
of
potassium
channels,
used
to
treat
angina,
has
drawn
attention
for
its
potential
in
mitigating
lung
injury,
positioning
it
as
promising
therapeutic
approach
drug-induced
toxicity.
This
study
aimed
explore
the
protective
role
nicorandil
arsenic
trioxide
(ATO)-induced
injury
and
elucidate
underlying
mechanistic
pathways.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(9), С. 1144 - 1144
Опубликована: Авг. 30, 2024
Hesperidin
(Hes)
functions
as
a
strong
antioxidant
and
anti-inflammatory
to
guard
against
damage
the
heart,
liver,
kidneys.
Nevertheless,
due
its
restricted
solubility
bioavailability,
delivery
method
is
required
for
it
reach
specific
organ.
In
this
study,
ion
gelation
was
used
synthesize
chitosan/hesperidin
nanoformulation.
Numerous
characterization
techniques,
such
zeta
potential,
particle
size,
XRD,
TEM,
SEM,
FTIR
analyses,
were
corroborate
synthesis
of
hesperidin
nanoparticles
(Hes-NPs).
Male
albino
mice
given
pretreatment
dose
100
mg/kg,
PO,
Hes
or
Hes-NPs,
which
administered
daily
14
days
before
induction
doxorubicin
nephrotoxicity
on
12th
day.
Kidney
function
(urea
creatinine
levels)
measured.
Lipid
peroxidation
(MDA)
enzyme
(CAT
SOD)
activities
estimated.
TNF-α,
IL-1β,
VEGF
content;
histopathological
examination
kidney
tissue;
immunohistochemical
staining
NF-κB,
Caspase-3,
BAX,
Bcl-2,
TGF-β1
evaluated.
The
gene
expressions
Free Radical Biology and Medicine,
Год журнала:
2023,
Номер
200, С. 11 - 25
Опубликована: Фев. 28, 2023
A
well-recognized
risk
factor
for
periodontitis,
diabetes
mellitus
(DM)
aggravates
periodontal
disease
with
increasing
alveolar
bone
loss.
As
a
novel
myokine,
irisin
is
closely
linked
metabolism.
Nonetheless,
the
effects
of
on
periodontitis
under
diabetic
conditions
and
underlying
mechanisms
remain
poorly
understood.
Here,
we
showed
that
local
treatment
ameliorates
loss
oxidative
stress,
increases
SIRT3
expression
within
tissues
our
experimentally-induced
(DP)
rat
models.
By
culturing
ligament
cells
(PDLCs)
in
vitro,
found
could
partially
rescue
inhibited
cell
viability,
mitigate
accumulated
intracellular
ameliorate
mitochondrial
dysfunctions,
restore
disturbed
osteogenic
osteoclastogenic
capacities
PDLCs
when
exposed
to
high
glucose
pro-inflammatory
stimulation.
Furthermore,
lentivirus-mediated
knockdown
was
employed
unravel
mechanism
by
which
mediated
irisin's
beneficial
PDLCs.
Meanwhile,
SIRT3-deficient
mice,
did
not
protect
against
destruction
stress
accumulation
DP
models,
underlined
crucial
role
mediating
positive
DP.
Our
findings,
first
time,
revealed
attenuates
via
activation
signaling
cascade,
highlighted
its
therapeutic
potential
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
170, С. 116006 - 116006
Опубликована: Дек. 12, 2023
Rheumatoid
arthritis
(RA)
is
a
chronic
inflammatory
condition
known
for
its
irreversible
destructive
impact
on
the
joints.
Chondrocytes
play
pivotal
role
in
production
and
maintenance
of
cartilage
matrix.
However,
presence
cytokines
can
hinder
chondrocyte
proliferation
promote
apoptosis.
Isoliquiritigenin
(ISL),
flavonoid,
potentially
exerts
protective
effects
against
various
diseases.
specific
regulating
nuclear
factor
E2-associated
2
(Nrf2)/heme
oxygenase-1
(HO-1)
pathway
chondrocytes
RA
remains
unclear.
To
investigate
this,
this
study
used
human
Sprague-Dawley
rats
to
construct
vitro
vivo
models,
respectively.
The
findings
reveal
that
markedly
induced
oxidative
stress,
activation
matrix
metalloproteinases,
apoptosis
both
vivo.
Notably,
ISL
treatment
significantly
mitigated
these
effects.
Moreover,
Nrf2
or
HO-1
inhibitors
reversed
ISL,
attenuated
expression
Nrf2/HO-1
peroxisome
proliferator-activated
receptor
gamma-coactivator-1α,
promoted
This
finding
indicates
primarily
targets
chondrocytes.
led
improved
behavior
scores,
reduced
paw
thickness,
joint
damage
as
well
ameliorated
stress
skeletal
muscles
an
rat
model.
In
conclusion,
highlights
demonstrates
potential
option
RA.
