Genomic prediction unifies animal and plant breeding programs to form platforms for biological discovery

Nature Genetics, Год журнала: 2017, Номер 49(9), С. 1297 - 1303

Опубликована: Сен. 1, 2017

Язык: Английский

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CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. Rasthali genome

Functional & Integrative Genomics, Год журнала: 2017, Номер 18(1), С. 89 - 99

Опубликована: Ноя. 29, 2017

Язык: Английский

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Application of genetics and biotechnology for improving medicinal plants

Planta, Год журнала: 2019, Номер 249(4), С. 953 - 973

Опубликована: Фев. 4, 2019

Язык: Английский

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First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(1), С. 396 - 396

Опубликована: Янв. 1, 2021

The current genome editing system Clustered Regularly Interspaced Short Palindromic Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and simplicity in several plant-based applications. Together with the availability of a vast amount data transcriptome data, CRISPR/Cas9 presents massive opportunity for plant breeders researchers. successful delivery ribonucleoproteins (RNPs), which are composed enzyme synthetically designed single guide RNA (sgRNA) used combination various transformation methods or lately available novel nanoparticle-based approaches, allows targeted mutagenesis plants species. Even though this technique is limitless, it still not been employed many species to date. Chickpea second most crucial winter grain crop cultivated worldwide; there currently no reports on gene chickpea. Here, we selected

Язык: Английский

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Plant drought stress tolerance: understanding its physiological, biochemical and molecular mechanisms

Biotechnology & Biotechnological Equipment, Год журнала: 2021, Номер 35(1), С. 1912 - 1925

Опубликована: Янв. 1, 2021

Plants are vulnerable to many environmental constraints which include drought, salinity, extreme temperatures, heavy metals, etc., thereby posing damage the development and yield of major crops. Of these stresses, drought represents a severe threat plant productivity in agriculture. Drought stress reduces by affecting key metabolic pathways. The capability plants switch on or off series genes result alterations physiological morphological attributes, allowing escape, tolerate avoid stress. Different genes, transcription factors signal transduction pathways induced Advances genome editing technologies have revolutionized agriculture sector using more accurate engineering techniques for targeted crop traits. Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is robust efficient approach developing plant/crop varieties tolerant different climatic changes. use new like CRISPR facilitates creation species with improved tolerance. Thus this review aims elucidate basic responses mechanisms adaptation attainment

Язык: Английский

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Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis

Nature Biotechnology, Год журнала: 2018, Номер 36(3), С. 249 - 257

Опубликована: Фев. 12, 2018

Язык: Английский

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Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

Frontiers in Plant Science, Год журнала: 2017, Номер 8

Опубликована: Апрель 20, 2017

Developing new ornamental cultivars with improved floral attributes is a major goal in floriculture. Biotechnological approach together classical breeding methods has been used to modify color, appearance as well for increasing disease resistance. Transgenic strategies possess immense potential produce novel flower phenotypes that are not found nature. Adoption of Genetic engineering supported the idea trait modification. Ornamental plant like fragrance, resistance and vase life can be by means genetic manipulation. Therefore, we witness transgenic varieties high aesthetic commercial value. This review focuses on biotechnological advancements manipulating key traits contribute development diverse lines. Data clearly reveals regulation biosynthetic pathways related characteristics pigment production, morphology fragrance both possible predictable. In spite their great significance, small number genetically engineered plants field tested. Today, colors production regarded chief benefit obtained from plants. But certain other much more important have potential. Other than achievements such architecture, modified etc. very few reports available regarding successful transformation valuable horticultural characteristics. Our also summarized efforts enhancement induction early flowering along changes anatomy morphology.

Язык: Английский

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Viral Vectors for Plant Genome Engineering

Frontiers in Plant Science, Год журнала: 2017, Номер 8

Опубликована: Апрель 11, 2017

Recent advances in genome engineering (GE) has made it possible to precisely alter DNA sequences plant cells, providing specifically engineered plants with traits of interest. Gene targeting (GT) efficiency depends on the delivery-method both sequence-specific nucleases (SSNs) and repair templates, cells. Typically, this is achieved using Agrobacterium mediated transformation or particle bombardment, which transform only a subset cells treated tissues. The alternate planta approaches, stably integrating nuclease-encoding cassettes templates into genome, are time consuming, expensive require extra regulations. More efficient GE reagents delivery methods clearly needed if GT become routine, especially economically important crops that difficult transform. Recently, autonomously replicating virus-based vectors have been demonstrated as means delivering plants. Both viruses (Bean yellow dwarf virus, Wheat virus Cabbage leaf curl virus) RNA (Tobacco rattle frequencies model (Nicotiana benthamiana) (potato, tomato, rice wheat). Here we discuss recent viral for engineering, current limitations future directions.

Язык: Английский

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Genome Editing in Plants: An Overview of Tools and Applications

International Journal of Agronomy, Год журнала: 2017, Номер 2017, С. 1 - 15

Опубликована: Янв. 1, 2017

The emergence of genome manipulation methods promises a real revolution in biotechnology and genetic engineering. Targeted editing the genomes living organisms not only permits investigations into understanding fundamental basis biological systems but also allows addressing wide range goals towards improving productivity quality crops. This includes creation plants with valuable compositional properties traits that confer resistance to various biotic abiotic stresses. During past few years, several novel have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs), clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). These exciting new methods, briefly reviewed herein, proved themselves as effective reliable tools for improvement plants.

Язык: Английский

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Plant genetic resources for food and agriculture: opportunities and challenges emerging from the science and information technology revolution

New Phytologist, Год журнала: 2018, Номер 217(4), С. 1407 - 1419

Опубликована: Янв. 23, 2018

Contents Summary 1407 I. Introduction 1408 II. Technological advances and their utility for gene banks breeding, longer‐term contributions to SDGs III. The challenges that must be overcome realise emerging R&D opportunities 1410 IV. Renewed governance structures PGR (and related big data) 1413 V. Access benefit sharing data 1416 VI. Conclusion 1417 Acknowledgements ORCID References Over the last decade, there has been an ongoing revolution in exploration, manipulation synthesis of biological systems, through development new technologies generate, analyse exploit data. Users Plant Genetic Resources ( ) can potentially leverage these capacities significantly increase efficiency effectiveness efforts conserve, discover utilise novel qualities , help achieve Sustainable Development Goals SDG s). This review discussion on discusses how taking advantage them will require integration across disciplinary, organisational international boundaries, formation multi‐disciplinary, partnerships. We explore some institutional policy face, particularly may influence structure role research sustainable development, ownership resources, access sharing. discuss potential responses political challenges, ranging from options enhanced discovery platforms internationally brokered benefit‐sharing agreements, identify a set broad principles could guide global community as it seeks or considers solutions.

Язык: Английский

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