Phyton,
Год журнала:
2023,
Номер
93(1), С. 81 - 96
Опубликована: Дек. 26, 2023
As
one
of
the
main
active
components
Dendrobium
catenatum,
alkaloids
have
high
medicinal
value.The
physicochemical
properties,
conserved
domains
and
motifs,
phylogenetic
analysis,
cis-acting
elements
gene
family
members
in
alkaloid
biosynthesis
pathway
D.
catenatum
were
analyzed
by
bioinformatics,
expression
genes
different
years
tissues
was
qRT-PCR.There
are
16
families,
including
25
genes,
pathway.The
analysis
motifs
showed
that
types,
quantities,
orders
similar
among
same
family,
but
there
significant
differences
families.Phylogenetic
indicated
some
evolutionary
conservation.Cis-acting
element
revealed
a
large
number
light-responsive
MYB
(v-myb
avian
myeloblastosis
viral
oncogene
homolog)-related
these
genes.qRT-PCR
expressions
involved
synthesis
catenatum.This
study
provides
theoretical
basis
for
further
exploration
regulatory
mechanisms
catenatum.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
25(1), С. 397 - 397
Опубликована: Дек. 28, 2023
The
sesquiterpene
alkaloid
dendrobine,
widely
recognized
as
the
main
active
compound
and
a
quality
control
standard
of
medicinal
orchids
in
Chinese
Pharmacopoeia,
demonstrates
diverse
biological
functions.
In
this
study,
we
engineered
Dendrobium
catenatum
chassis
plant
for
production
dendrobine
through
screening
pyramiding
key
biosynthesis
genes.
Initially,
previously
predicted
upstream
genes
methyl-D-erythritol
4-phosphate
(MEP)
pathway
synthesis,
including
4-(Cytidine
5'-Diphospho)-2-C-Methyl-d-Erythritol
Kinase
(CMK),
1-Deoxy-d-Xylulose
5-Phosphate
Reductoisomerase
(DXR),
2-C-Methyl-d-Erythritol
4-Phosphate
Cytidylyltransferase
(MCT),
Strictosidine
Synthase
1
(STR1),
few
downstream
post-modification
genes,
Cytochrome
P450
94C1
(CYP94C1),
Branched-Chain-Amino-Acid
Aminotransferase
2
(BCAT2),
Methyltransferase-like
Protein
23
(METTL23),
were
chosen
due
to
their
deduced
roles
enhancing
production.
seven
(SG)
then
stacked
transiently
expressed
leaves
D.
catenatum,
resulting
yield
that
was
two-fold
higher
compared
empty
vector
(EV).
Further,
RNA-seq
analysis
identified
Copper
Methylamine
Oxidase
(CMEAO)
strong
candidate
with
functions
processes
biosynthesis.
Overexpression
CMEAO
increased
content
by
two-fold.
Additionally,
co-expression
differentially
(DEGs)
weighted
gene
network
(WGCNA)
retrieved
one
regulatory
transcription
factor
MYB61.
MYB61
levels
more
than
catenatum.
short,
work
provides
an
efficient
strategy
prospective
candidates
genetic
engineering
produce
thereby
improving
its
value.
Molecules,
Год журнала:
2022,
Номер
27(21), С. 7233 - 7233
Опубликована: Окт. 25, 2022
Dendrobium
is
the
second
biggest
genus
in
Orchidaceae
family,
and
many
of
them
have
been
utilized
as
a
traditional
Chinese
medicine
(TCM)
for
thousands
years
China.
In
last
few
decades,
constituents
with
great
chemical
diversity
were
isolated
from
Dendrobium,
wide
range
biological
activities
detected,
either
crude
extracts
or
pure
compounds.
Stilbene
compound
one
primary
active
Dendrobium.
At
present,
267
stilbene
compounds
clarified
molecular
structures
extracted
52
species
including
124
phenanthrenes
143
bibenzyls.
same
time,
activity
studies
indicated
that
157
pharmaceutical
activity.
Among
them,
most
showed
antitumor
activity,
followed
by
antioxidant,
anti-inflammatory
anti-α-glucosidase
inhibitory
activities.
Additionally,
54
multiple
pharmacological
activities,
such
confusarin
(14),
2,4,7-trihydroxy-9,10-dihydro-phenanthrene
(43),
moscatilin
(148),
gigantol
(150)
batatasin
III
(151).
This
review
summarizes
current
knowledge
about
composition
stilbene,
bioactivities
pharmacologic
effects
We
also
expect
to
provide
reference
further
research,
development
utilization
genus.
Frontiers in Plant Science,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 4, 2022
Jasmonate
(JA)
signaling
plays
a
pivotal
role
in
plant
stress
responses
and
secondary
metabolism.
Many
studies
have
demonstrated
that
JA
effectively
induce
the
expressions
of
alkaloid
biosynthetic
genes
various
plants,
which
rendered
to
accumulation
counteract
stresses.
Despite
multiple
roles
regulation
growth
different
stresses,
less
studied
involved
regulatory
Dendrobium
officinale
alkaloids.
A
strategy
for
rapid
identification
intermediates
D.
was
established
based
on
solid-phase
extraction
coupled
with
high-performance
liquid
chromatography
tandem
mass
spectrometry
method.
By
using
SPE-LC-MS/MS
method,
potential
compounds
were
tentatively
identified
by
aligning
accurate
molecular
weight
METLIN
Dictionary
Natural
Products
databases.
The
chemical
structures
main
characteristic
fragments
further
confirmed
retrieving
multistage
spectra
from
MassBank
Mass
Frontier
software
used
speculate
fragmentation
pathway
compounds.
Seven
alkaloids
separated
officinale,
mainly
classified
into
five
types
(tropane
alkaloids,
tetrahydroisoquinoline
quinolizidine
piperidine
spermidine
alkaloids).
Besides
forty-nine
substances,
including
guanidines,
nucleotides,
dipeptides,
sphingolipids
nitrogen-containing
glucosides,
concurrently
identified.
These
findings
gives
composition
chemicals
currently
found
could
provide
scientific
method
other
plants.
