Identification and Molecular Characterization of the Alkaloid Biosynthesis Gene Family in <i>Dendrobium catenatum</i> DOI Open Access
Liping Yang, Xin Wan,

Runyang Zhou

et al.

Phyton, Journal Year: 2023, Volume and Issue: 93(1), P. 81 - 96

Published: Dec. 26, 2023

As one of the main active components Dendrobium catenatum, alkaloids have high medicinal value.The physicochemical properties, conserved domains and motifs, phylogenetic analysis, cis-acting elements gene family members in alkaloid biosynthesis pathway D. catenatum were analyzed by bioinformatics, expression genes different years tissues was qRT-PCR.There are 16 families, including 25 genes, pathway.The analysis motifs showed that types, quantities, orders similar among same family, but there significant differences families.Phylogenetic indicated some evolutionary conservation.Cis-acting element revealed a large number light-responsive MYB (v-myb avian myeloblastosis viral oncogene homolog)-related these genes.qRT-PCR expressions involved synthesis catenatum.This study provides theoretical basis for further exploration regulatory mechanisms catenatum.

Language: Английский

Transcriptome and metabolome analysis reveal the dynamic changes and biosynthesis pathways of alkaloids in Sophora alopecuroides L. under drought stress DOI
Xiang Huang,

Wenwen Rong,

Xingxin Zhang

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 212, P. 118365 - 118365

Published: March 7, 2024

Language: Английский

Citations

15
Exploring the mechanism of dendrobine in treating metabolic associated fatty liver disease based on network pharmacology and experimental validation DOI Creative Commons
Feng Li, Jialin Wu, Ye Zhu

et al.

Hereditas, Journal Year: 2024, Volume and Issue: 161(1)

Published: May 16, 2024

Abstract Background This study investigates the therapeutic mechanisms of dendrobine, a primary bioactive compound in Dendrobium nobile, for Metabolic Associated Fatty Liver Disease (MASLD) management. Utilizing network pharmacology combined with experimental validation, clinical effectiveness dendrobine MASLD treatment was assessed and analyzed. Results The demonstrates significant improvement liver function among patients treated nobile. Network identified key targets such as Peroxisome Proliferator-Activated Receptor Gamma (PPARG), Interleukin 6 (IL6), Tumor Necrosis Factor (TNF), 1 Beta (IL1B), AKT Serine/Threonine Kinase (AKT1), molecular docking confirming their interactions. Additionally, significantly reduced ALT AST levels palmitic acid-treated HepG2 cells, indicating hepatoprotective properties amelioration oxidative stress through decreased Malondialdehyde (MDA) increased Superoxide Dismutase (SOD) levels. Conclusion Dendrobine mitigates damage modulating inflammatory immune responses affecting lipid metabolism, potentially by downregulating mediators like TNF, IL6, IL1B, inhibiting AKT1 Signal Transducer Activator Transcription 3 (STAT3). provides theoretical basis application treatment, highlighting its potential agent.

Language: Английский

Citations

4
Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity DOI
Yichen Liu, Arome Solomon Odiba,

Qi Yu

et al.

Journal of Future Foods, Journal Year: 2025, Volume and Issue: 5(6), P. 591 - 604

Published: Jan. 29, 2025

Language: Английский

Citations

0
Dynamic Changes in Functional Components of Dendrobium officinale and Their Applications in Food Science: A Review DOI
Mei Wang,

Gaige Shao,

Minquan Song

et al.

Plant Foods for Human Nutrition, Journal Year: 2025, Volume and Issue: 80(1)

Published: Feb. 10, 2025

Language: Английский

Citations

0
Role of Biotechnology in the Production of Health Promising Bioactive Molecules DOI
Munish Sharma, Munit Sharma, Shalini Pandey

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Differential involvement of WRKY genes in abiotic stress tolerance of Dendrobium huoshanense DOI
Yingyu Zhang,

Wenwu Zhang,

Muhammad Aamir Manzoor

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 204, P. 117295 - 117295

Published: Aug. 11, 2023

Language: Английский

Citations

10
Metabolic Pathway Engineering Improves Dendrobine Production in Dendrobium catenatum DOI Open Access
Meili Zhao,

Yanchang Zhao,

Zhenyu Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 397 - 397

Published: Dec. 28, 2023

The sesquiterpene alkaloid dendrobine, widely recognized as the main active compound and a quality control standard of medicinal orchids in Chinese Pharmacopoeia, demonstrates diverse biological functions. In this study, we engineered Dendrobium catenatum chassis plant for production dendrobine through screening pyramiding key biosynthesis genes. Initially, previously predicted upstream genes methyl-D-erythritol 4-phosphate (MEP) pathway synthesis, including 4-(Cytidine 5'-Diphospho)-2-C-Methyl-d-Erythritol Kinase (CMK), 1-Deoxy-d-Xylulose 5-Phosphate Reductoisomerase (DXR), 2-C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase (MCT), Strictosidine Synthase 1 (STR1), few downstream post-modification genes, Cytochrome P450 94C1 (CYP94C1), Branched-Chain-Amino-Acid Aminotransferase 2 (BCAT2), Methyltransferase-like Protein 23 (METTL23), were chosen due to their deduced roles enhancing production. seven (SG) then stacked transiently expressed leaves D. catenatum, resulting yield that was two-fold higher compared empty vector (EV). Further, RNA-seq analysis identified Copper Methylamine Oxidase (CMEAO) strong candidate with functions processes biosynthesis. Overexpression CMEAO increased content by two-fold. Additionally, co-expression differentially (DEGs) weighted gene network (WGCNA) retrieved one regulatory transcription factor MYB61. MYB61 levels more than catenatum. short, work provides an efficient strategy prospective candidates genetic engineering produce thereby improving its value.

Language: Английский

Citations

10
Piperidine polyketide alkaloids of bacterial origin: occurrence, bioactivity, and biosynthesis DOI
Xin Xiong,

Xiaofan Zhu,

Daejung Yu

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 289, P. 117498 - 117498

Published: March 10, 2025

Language: Английский

Citations

0
Recent Research Progress on Natural Stilbenes in Dendrobium Species DOI Creative Commons
Denghui Zhai,

Xiaofa Lv,

Chen Jing-mei

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(21), P. 7233 - 7233

Published: Oct. 25, 2022

Dendrobium is the second biggest genus in Orchidaceae family, and many of them have been utilized as a traditional Chinese medicine (TCM) for thousands years China. In last few decades, constituents with great chemical diversity were isolated from Dendrobium, wide range biological activities detected, either crude extracts or pure compounds. Stilbene compound one primary active Dendrobium. At present, 267 stilbene compounds clarified molecular structures extracted 52 species including 124 phenanthrenes 143 bibenzyls. same time, activity studies indicated that 157 pharmaceutical activity. Among them, most showed antitumor activity, followed by antioxidant, anti-inflammatory anti-α-glucosidase inhibitory activities. Additionally, 54 multiple pharmacological activities, such confusarin (14), 2,4,7-trihydroxy-9,10-dihydro-phenanthrene (43), moscatilin (148), gigantol (150) batatasin III (151). This review summarizes current knowledge about composition stilbene, bioactivities pharmacologic effects We also expect to provide reference further research, development utilization genus.

Language: Английский

Citations

15
Identification of alkaloids and related intermediates of Dendrobium officinale by solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry DOI Creative Commons
Cheng Song, Yunpeng Zhang, Muhammad Aamir Manzoor

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 4, 2022

Jasmonate (JA) signaling plays a pivotal role in plant stress responses and secondary metabolism. Many studies have demonstrated that JA effectively induce the expressions of alkaloid biosynthetic genes various plants, which rendered to accumulation counteract stresses. Despite multiple roles regulation growth different stresses, less studied involved regulatory Dendrobium officinale alkaloids. A strategy for rapid identification intermediates D. was established based on solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry method. By using SPE-LC-MS/MS method, potential compounds were tentatively identified by aligning accurate molecular weight METLIN Dictionary Natural Products databases. The chemical structures main characteristic fragments further confirmed retrieving multistage spectra from MassBank Mass Frontier software used speculate fragmentation pathway compounds. Seven alkaloids separated officinale, mainly classified into five types (tropane alkaloids, tetrahydroisoquinoline quinolizidine piperidine spermidine alkaloids). Besides forty-nine substances, including guanidines, nucleotides, dipeptides, sphingolipids nitrogen-containing glucosides, concurrently identified. These findings gives composition chemicals currently found could provide scientific method other plants.

Language: Английский

Citations

14