Distribution of HPV Types in Cervical Cancer in Pakistan: Implications for Screening and Vaccination Programs DOI Creative Commons
Hafsa Aziz,

Humera Mehmood,

Sarosh Arif

и другие.

Journal of Clinical Laboratory Analysis, Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

ABSTRACT Background HPV plays a key role in the development of cervical cancer. This study aims to investigate prevalence genotypes patients with Squamous cell carcinoma (SSC) and Adenocarcinoma (ADC) at NORI cancer Hospital Pakistan, aim improving screening prevention strategies. Method Cervical scrapings were collected from 129 diagnosed patients. typing was performed using real‐time PCR assay sequencing. Result Among patients, 73.6% (90/129) positive. Proportion positivity observed within each group. The highest incidence 50–60 years age group (80.9%), followed by 40‐to‐50‐year (75.8%). rate declined 60‐to‐70‐year‐old (63.6%) further 70–80 (62.5%). Eight different subtypes identified, 16 being most prevalent (80.0%), 18 (9.5%), 45 2 (2.1%), 31 1 (1.1%), 35 59 66 89 (1.15). Histologically, 89.2% cases (SCC) 10.8% Adenocarcinomas. In SCC found 80.9% cases, while detected 66.7% cases. Conclusion high‐risk types, both vaccine non‐vaccine, targeted Pakistan highlights urgent need for widespread vaccination programs. Tailored public health strategies are essential effectively reduce rates mortality.

Язык: Английский

Prevailing of HPV-16 and 52 genotype in 2022–2023 in Sanandaj, Iran DOI Creative Commons
Mohammad Haddadi,

Leila Atefmehr,

Saeed Motlaghzadeh

и другие.

Virology Journal, Год журнала: 2024, Номер 21(1)

Опубликована: Май 7, 2024

Abstract Introduction Human papillomavirus (HPV) presents a potential threat to the onset of carcinogenesis in cervix, anogenital regions, and oropharynx. HPV encompasses over 200 types, with at least 12 having cause cancer, impacting majority sexually active individuals. In this current research, we explore occurrence spread genotypes. Material methods During cross-sectional study conducted Sanandaj, Iran from Feb 2022 Aug 2023, diverse samples including oral, vaginal, genital were collected individuals referred private laboratories Iran. After sample collection DNA extraction (FAVORGEN, Taiwan), they subjected PCR genotyping (MehrViru, Iran). The subsequent statistical analysis unveiled infection rates across different demographics age groups. STATA (version 17) used for analysis. We examined using t-tests Odds Ratio. Results Overall, 26% (249) out 950 cases tested positive HPV, 69% these classified as high-risk. Among population, 98% (933) female, 2% (17) male. Females aged 31–40 exhibited highest percentage prevalence (115/460) belonging HR overall most frequent genotypes identified 6, 16, 52, 53, 51, 58, 56. HPV-16 frequency among genotypes, accounting 42 (17%) occurrences, followed by HPV-52 32 (13%). Conclusion Our findings emphasize significant females, particularly 21–30 group. identification high-risk underscores importance targeted interventions specific cohorts. age-stratified highlights consistent predominance groups, indicating need age-specific preventive measures. These results contribute valuable information designing effective screening vaccination strategies, alleviate impact diseases associated HPV.

Язык: Английский

Процитировано

6

Rational design of a triple-type HPV53/56/66 vaccine with one preferable base particle incorporating two identified immunodominant sites DOI Creative Commons
Ciying Qian, Jie Chen, Yurou Yang

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 20, 2025

The numerous high-risk carcinogenic types of human papillomavirus (HR-HPV) that lack vaccine protection underscore the urgent need to develop broader-spectrum HPV vaccines. This study addresses this by focusing on HR-HPV 53, 56, and 66, which are not currently targeted existing It introduces an effective method for their soluble expression, as well mutants, within Escherichia coli expression system. Through strategic homologous loop swapping among HPV53, HPV56, HPV66, we designed twenty double-type chimeric molecules. Comprehensive evaluations identified unique dominant immunogenic loops each type: FG HI DE with HPV66 emerging optimal backbone virus-like particle (VLP). By incorporating two immunodominant sites into preferable base particle, constructed a triple-type chimera H66-56HI-53FG, could efficiently self-assemble VLPs in vitro closely resembled wild-type VLP and, induced balanced neutralization titers (~ 3 log unites), contrast none observable HPV53 titer lower HPV56 elicited immunization alone. research outlines amenable way simultaneously identify context cross-type design, thereby offering paradigm extending antigenic variety single broaden coverage.

Язык: Английский

Процитировано

0

Clinical applications and utility of ctDNA in cervical cancer and its precursor lesions: from screening to predictive biomarker DOI Creative Commons
Li Li, Yixin Tong, Jian Wu

и другие.

Cancer Cell International, Год журнала: 2023, Номер 23(1)

Опубликована: Дек. 18, 2023

Abstract Cervical cancer is a leading cause of gynecological death in the world. Human papillomavirus (HPV) most causative factor cervical cancer. In addition, many genetic factors are involved development. Most studies focus on samples to do research work about and precancerous lesions, but no sensitive or specific biomarkers were found. High-throughput genomic technologies able capture information from tumors lesions blood, thus providing new way for early diagnosis precancer Blood an ideal specimen detecting because it contains lot information, such as circulating tumor cells DNA (ctDNA). This article reviews clinical use challenges blood ctDNA testing patients with

Язык: Английский

Процитировано

4

Diagnostic validity of p16, E-cadherin, cyclin D1, p53, and HPV E6/E7 mRNA in CIN 3-like squamous cell carcinoma of the cervix DOI Creative Commons
Xing Zhao,

Shen Danhua,

Xiaobo Zhang

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Янв. 26, 2024

Objective Cervical intraepithelial neoplasia grade 3 (CIN 3)-like SCC is a recently identified deceptive growth pattern that closely mimics endocervical crypt involvement by CIN 3. As 3-like indistinguishable from 3, it poses significant challenge for pathologists. Method We examined 23 cases of SCC, 6 which also had concomitant conventional invasive and 9 as control group. Immunohistochemistry was used to investigate the expression p16, E-cadherin, cyclin D1, p53, human papillomavirus (HPV) E6/E7 mRNA, key virus carcinogen HPV, detected. The clinicopathological, immunohistochemical, molecular characteristics element were examined. Result exhibited characteristic morphology similar with pushing borders invading into wall cervix, often depth in most cases. Immunophenotypic features p53 differed between both staining intensity region. mRNA higher than (P < 0.05). Conclusion type cancer, presenting numerous challenges potential confusion phenotypes

Язык: Английский

Процитировано

1

High prevalence of HPV-56 and HPV-39 in Sari, Iran: an analysis of genotype distribution DOI Creative Commons
Arash Letafati, Ali Vasheghani Farahani,

Mohammad Mostafa Baradaran Nasiri

и другие.

Virology Journal, Год журнала: 2024, Номер 21(1)

Опубликована: Сен. 20, 2024

Язык: Английский

Процитировано

1

The interplay between programmed death ligand 1 (PD-L1) expression and human papilloma virus (HPV) genotypes in cervical carcinomas: findings of a Nigerian Tertiary Hospital DOI Creative Commons
Sebastian A. Omenai, Mustapha Ajani

Pan African Medical Journal, Год журнала: 2024, Номер 48

Опубликована: Янв. 1, 2024

cervical cancer is primarily driven by high-risk human papillomavirus infections. It a leading cause of cancer-related deaths among women globally. The emergence immunotherapeutic approaches, particularly programmed death ligand-1 (PD-L1) inhibitors, has shown promise in various cancers. This study aims to investigate the correlation between PD-L1 expression and HPV status carcinoma samples from Nigerian tertiary hospital.

Язык: Английский

Процитировано

0

Profiling of the Retrospective Epidemiology of the Human Papillomavirus and Related Cancers in the United Arab Emirates Population DOI Open Access

Ihsan Ali Mahasneh,

Berjas Abumusimir,

Mustapha Enaji

и другие.

Journal of Biosciences and Medicines, Год журнала: 2024, Номер 12(10), С. 1 - 9

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Distribution of HPV genotypes in Mashhad, Iran: insights from a 2022–2023 study DOI Creative Commons
Arash Letafati,

Masoomeh Noroozi,

Tina Fallah

и другие.

Virology Journal, Год журнала: 2024, Номер 21(1)

Опубликована: Окт. 7, 2024

Human papillomavirus (HPV), is one of the main causes cervical cancer and also most common sexually transmitted infections (STIs). HPV responsible for almost all cases plays a principal role in causing other cancers including oropharynx, penis, larynx, oral cavity, anus, vulva, vagina. The study aims to investigate prevalence distribution genotypes among patients referred private laboratories Mashhad, located northeast Iran.

Язык: Английский

Процитировано

0

Trends in high-risk human papillomavirus infection and cervical cytology of women in Karamay City, 2012–2021 DOI

Xiaoping Jia,

Min Jiang, Jing Zhou

и другие.

Sexual Health, Год журнала: 2024, Номер 21(6)

Опубликована: Окт. 22, 2024

Background To describe the changes in cervical lesions prevalence and high-risk human papillomavirus (HR-HPV) infections from 2012 to 2021, which have never been reported before, provide direction for effective implementation of cancer prevention measures. Methods This retrospective study included women aged >25 years who received either organised or opportunistic HR-HPV screening January December 2021 Karamay Central Hospital, Karamay, China. The patients were split into four groups according age 25–35, 36–45, 46–55 >55 years, respectively. Joinpoint Regression Program was used analyse trends infection detection lesions. Results Data 85,429 revealed a decline rates across all 2021. Although decreased, lesion increased, although proportion these declined, likely due enhanced awareness HPV vaccination Karamay. From low-grade squamous intraepithelial 9.70%, high-grade 5.85%. highest ≥55 group, with HPV52 (20.96%) being most prevalent type. Conclusions In past 10 has shown decreasing trend, whereas an upward trend among City. Importantly, particular emphasis should be placed on years.

Язык: Английский

Процитировано

0

Distribution of HPV Types in Cervical Cancer in Pakistan: Implications for Screening and Vaccination Programs DOI Creative Commons
Hafsa Aziz,

Humera Mehmood,

Sarosh Arif

и другие.

Journal of Clinical Laboratory Analysis, Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

ABSTRACT Background HPV plays a key role in the development of cervical cancer. This study aims to investigate prevalence genotypes patients with Squamous cell carcinoma (SSC) and Adenocarcinoma (ADC) at NORI cancer Hospital Pakistan, aim improving screening prevention strategies. Method Cervical scrapings were collected from 129 diagnosed patients. typing was performed using real‐time PCR assay sequencing. Result Among patients, 73.6% (90/129) positive. Proportion positivity observed within each group. The highest incidence 50–60 years age group (80.9%), followed by 40‐to‐50‐year (75.8%). rate declined 60‐to‐70‐year‐old (63.6%) further 70–80 (62.5%). Eight different subtypes identified, 16 being most prevalent (80.0%), 18 (9.5%), 45 2 (2.1%), 31 1 (1.1%), 35 59 66 89 (1.15). Histologically, 89.2% cases (SCC) 10.8% Adenocarcinomas. In SCC found 80.9% cases, while detected 66.7% cases. Conclusion high‐risk types, both vaccine non‐vaccine, targeted Pakistan highlights urgent need for widespread vaccination programs. Tailored public health strategies are essential effectively reduce rates mortality.

Язык: Английский

Процитировано

0