Journal of Clinical Laboratory Analysis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 5, 2024
ABSTRACT
Background
HPV
plays
a
key
role
in
the
development
of
cervical
cancer.
This
study
aims
to
investigate
prevalence
genotypes
patients
with
Squamous
cell
carcinoma
(SSC)
and
Adenocarcinoma
(ADC)
at
NORI
cancer
Hospital
Pakistan,
aim
improving
screening
prevention
strategies.
Method
Cervical
scrapings
were
collected
from
129
diagnosed
patients.
typing
was
performed
using
real‐time
PCR
assay
sequencing.
Result
Among
patients,
73.6%
(90/129)
positive.
Proportion
positivity
observed
within
each
group.
The
highest
incidence
50–60
years
age
group
(80.9%),
followed
by
40‐to‐50‐year
(75.8%).
rate
declined
60‐to‐70‐year‐old
(63.6%)
further
70–80
(62.5%).
Eight
different
subtypes
identified,
16
being
most
prevalent
(80.0%),
18
(9.5%),
45
2
(2.1%),
31
1
(1.1%),
35
59
66
89
(1.15).
Histologically,
89.2%
cases
(SCC)
10.8%
Adenocarcinomas.
In
SCC
found
80.9%
cases,
while
detected
66.7%
cases.
Conclusion
high‐risk
types,
both
vaccine
non‐vaccine,
targeted
Pakistan
highlights
urgent
need
for
widespread
vaccination
programs.
Tailored
public
health
strategies
are
essential
effectively
reduce
rates
mortality.
Journal of Nanobiotechnology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: Jan. 20, 2025
The
numerous
high-risk
carcinogenic
types
of
human
papillomavirus
(HR-HPV)
that
lack
vaccine
protection
underscore
the
urgent
need
to
develop
broader-spectrum
HPV
vaccines.
This
study
addresses
this
by
focusing
on
HR-HPV
53,
56,
and
66,
which
are
not
currently
targeted
existing
It
introduces
an
effective
method
for
their
soluble
expression,
as
well
mutants,
within
Escherichia
coli
expression
system.
Through
strategic
homologous
loop
swapping
among
HPV53,
HPV56,
HPV66,
we
designed
twenty
double-type
chimeric
molecules.
Comprehensive
evaluations
identified
unique
dominant
immunogenic
loops
each
type:
FG
HI
DE
with
HPV66
emerging
optimal
backbone
virus-like
particle
(VLP).
By
incorporating
two
immunodominant
sites
into
preferable
base
particle,
constructed
a
triple-type
chimera
H66-56HI-53FG,
could
efficiently
self-assemble
VLPs
in
vitro
closely
resembled
wild-type
VLP
and,
induced
balanced
neutralization
titers
(~
3
log
unites),
contrast
none
observable
HPV53
titer
lower
HPV56
elicited
immunization
alone.
research
outlines
amenable
way
simultaneously
identify
context
cross-type
design,
thereby
offering
paradigm
extending
antigenic
variety
single
broaden
coverage.
Virology Journal,
Journal Year:
2024,
Volume and Issue:
21(1)
Published: May 7, 2024
Abstract
Introduction
Human
papillomavirus
(HPV)
presents
a
potential
threat
to
the
onset
of
carcinogenesis
in
cervix,
anogenital
regions,
and
oropharynx.
HPV
encompasses
over
200
types,
with
at
least
12
having
cause
cancer,
impacting
majority
sexually
active
individuals.
In
this
current
research,
we
explore
occurrence
spread
genotypes.
Material
methods
During
cross-sectional
study
conducted
Sanandaj,
Iran
from
Feb
2022
Aug
2023,
diverse
samples
including
oral,
vaginal,
genital
were
collected
individuals
referred
private
laboratories
Iran.
After
sample
collection
DNA
extraction
(FAVORGEN,
Taiwan),
they
subjected
PCR
genotyping
(MehrViru,
Iran).
The
subsequent
statistical
analysis
unveiled
infection
rates
across
different
demographics
age
groups.
STATA
(version
17)
used
for
analysis.
We
examined
using
t-tests
Odds
Ratio.
Results
Overall,
26%
(249)
out
950
cases
tested
positive
HPV,
69%
these
classified
as
high-risk.
Among
population,
98%
(933)
female,
2%
(17)
male.
Females
aged
31–40
exhibited
highest
percentage
prevalence
(115/460)
belonging
HR
overall
most
frequent
genotypes
identified
6,
16,
52,
53,
51,
58,
56.
HPV-16
frequency
among
genotypes,
accounting
42
(17%)
occurrences,
followed
by
HPV-52
32
(13%).
Conclusion
Our
findings
emphasize
significant
females,
particularly
21–30
group.
identification
high-risk
underscores
importance
targeted
interventions
specific
cohorts.
age-stratified
highlights
consistent
predominance
groups,
indicating
need
age-specific
preventive
measures.
These
results
contribute
valuable
information
designing
effective
screening
vaccination
strategies,
alleviate
impact
diseases
associated
HPV.
Virology Journal,
Journal Year:
2025,
Volume and Issue:
22(1)
Published: May 22, 2025
Understanding
the
regional
HPV
genotype
profile
is
critical
for
informing
targeted
vaccination
strategies
and
optimizing
cervical
cancer
screening
programs
to
enhance
their
effectiveness.
This
study
investigated
prevalence
distribution
of
human
papillomavirus
(HPV)
genotypes
among
women
with
squamous
cell
carcinoma
(CSCC)
in
Linyi
city,
China,
from
2015
2023.
Data
were
obtained
606
histologically
diagnosed
CSCC
at
Cancer
Hospital
between
January
December
DNA
was
extracted
paraffin-embedded
tissue
samples.
genotyping
performed
via
gene
chip-based
polymerase
chain
reaction
(PCR)
technology.
Temporal
trends
age-specific
variations
analyzed
provide
a
comprehensive
epidemiological
assessment.
The
overall
infection
94.7%
CSCC.
16
most
prevalent
(80.5%),
followed
by
18
(5.2%),
33
(2.8%),
31
(1.8%),
58
(1.8%).
Single
infections
predominant
(95.5%),
while
coinfections
observed
4.5%
cases.
Age-specific
analysis
revealed
that
non-HPV
more
aged
>
45
years,
greater
diversity
older
age
groups.
indicated
decline
younger
patients
(26-45
years),
whereas
significantly
increased.
Our
varies
age,
highlighting
need
age-stratified
personalized
prevention
strategies.
Enhanced
efforts
are
essential
because
this
group.
Additionally,
over
time
suggest
has
effectively
reduced
incidence
under
years
age.
These
findings
emphasize
importance
expanding
coverage
further
reduce
burden
across
different
Infectious Agents and Cancer,
Journal Year:
2025,
Volume and Issue:
20(1)
Published: May 25, 2025
Abstract
Background
and
aim
Persistent
infections
with
high-risk
human
papillomavirus
(HR-HPV)
are
linked
to
cervical
cancer
progression.
The
prevalence
distribution
of
HPV
genotypes
vary
across
regions
lesion
severity.
Comprehensive
data
on
genotype
among
Iranian
women
is
limited.
This
study
investigates
the
HR-HPV
in
abnormal
cytology
Qazvin
province,
northwest
Iran,
from
2007
2019.
Materials
methods
A
total
103
samples,
including
benign
cases,
Low-grade
Squamous
Intraepithelial
Lesions
(LSIL),
High-grade
(HSIL),
Invasive
Cervical
Cancer
(ICC),
were
analyzed
using
real-time
PCR
detect
types
16,
18,
31,
33,
35,
39,
45,
51,
52,
58,
59.
Results
revealed
a
high
(92.23%),
HPV-16
being
most
common
(66.31%),
followed
by
HPV-45
(49.47%),
HPV-33
(41.05%),
HPV-31(30.52%)
HPV-52
(23.15%).
HPV-18
was
detected
only
3
(3.15%)
cases.
Of
HPV-positive
82.11%
had
multiple
infections,
HPV-16,
HPV-33,
more
prevalent
these
significantly
associated
severe
lesions,
particularly
ICC
cases
(92%,
P
=
0.007).
Conclusion
These
findings
emphasize
role
genotyping
assessing
severity
oncogenic
risk,
highlighting
as
dominant
various
grades.
suggests
that
may
also
contribute
Cancer Cell International,
Journal Year:
2023,
Volume and Issue:
23(1)
Published: Dec. 18, 2023
Abstract
Cervical
cancer
is
a
leading
cause
of
gynecological
death
in
the
world.
Human
papillomavirus
(HPV)
most
causative
factor
cervical
cancer.
In
addition,
many
genetic
factors
are
involved
development.
Most
studies
focus
on
samples
to
do
research
work
about
and
precancerous
lesions,
but
no
sensitive
or
specific
biomarkers
were
found.
High-throughput
genomic
technologies
able
capture
information
from
tumors
lesions
blood,
thus
providing
new
way
for
early
diagnosis
precancer
Blood
an
ideal
specimen
detecting
because
it
contains
lot
information,
such
as
circulating
tumor
cells
DNA
(ctDNA).
This
article
reviews
clinical
use
challenges
blood
ctDNA
testing
patients
with
Frontiers in Oncology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 26, 2024
Objective
Cervical
intraepithelial
neoplasia
grade
3
(CIN
3)-like
SCC
is
a
recently
identified
deceptive
growth
pattern
that
closely
mimics
endocervical
crypt
involvement
by
CIN
3.
As
3-like
indistinguishable
from
3,
it
poses
significant
challenge
for
pathologists.
Method
We
examined
23
cases
of
SCC,
6
which
also
had
concomitant
conventional
invasive
and
9
as
control
group.
Immunohistochemistry
was
used
to
investigate
the
expression
p16,
E-cadherin,
cyclin
D1,
p53,
human
papillomavirus
(HPV)
E6/E7
mRNA,
key
virus
carcinogen
HPV,
detected.
The
clinicopathological,
immunohistochemical,
molecular
characteristics
element
were
examined.
Result
exhibited
characteristic
morphology
similar
with
pushing
borders
invading
into
wall
cervix,
often
depth
in
most
cases.
Immunophenotypic
features
p53
differed
between
both
staining
intensity
region.
mRNA
higher
than
(P
<
0.05).
Conclusion
type
cancer,
presenting
numerous
challenges
potential
confusion
phenotypes
Pan African Medical Journal,
Journal Year:
2024,
Volume and Issue:
48
Published: Jan. 1, 2024
cervical
cancer
is
primarily
driven
by
high-risk
human
papillomavirus
infections.
It
a
leading
cause
of
cancer-related
deaths
among
women
globally.
The
emergence
immunotherapeutic
approaches,
particularly
programmed
death
ligand-1
(PD-L1)
inhibitors,
has
shown
promise
in
various
cancers.
This
study
aims
to
investigate
the
correlation
between
PD-L1
expression
and
HPV
status
carcinoma
samples
from
Nigerian
tertiary
hospital.
Virology Journal,
Journal Year:
2024,
Volume and Issue:
21(1)
Published: Oct. 7, 2024
Human
papillomavirus
(HPV),
is
one
of
the
main
causes
cervical
cancer
and
also
most
common
sexually
transmitted
infections
(STIs).
HPV
responsible
for
almost
all
cases
plays
a
principal
role
in
causing
other
cancers
including
oropharynx,
penis,
larynx,
oral
cavity,
anus,
vulva,
vagina.
The
study
aims
to
investigate
prevalence
distribution
genotypes
among
patients
referred
private
laboratories
Mashhad,
located
northeast
Iran.