Electroactive Biomaterials Regulate the Electrophysiological Microenvironment to Promote Bone and Cartilage Tissue Regeneration

Advanced Functional Materials, Год журнала: 2024, Номер 34(23)

Опубликована: Янв. 7, 2024

Abstract The incidence of large bone and articular cartilage defects caused by traumatic injury is increasing worldwide; the tissue regeneration process for these injuries lengthy due to limited self‐healing ability. Endogenous bioelectrical phenomenon has been well recognized play an important role in homeostasis regeneration. Studies have reported that electrical stimulation (ES) can effectively regulate various biological processes holds promise as external intervention enhance synthesis extracellular matrix, thereby accelerating Hence, electroactive biomaterials considered a biomimetic approach ensure functional recovery integrating physiological signals, including electrical, biochemical, mechanical signals. This review will discuss endogenous bioelectricity tissue, effects ES on cellular behaviors. Then, recent advances materials their applications are systematically overviewed, with focus advantages disadvantages repair performances modulation cell fate. Finally, significance mimicking electrophysiological microenvironment target emphasized future development challenges strategies proposed.

Язык: Английский

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Exploring the translational potential of PLGA nanoparticles for intra-articular rapamycin delivery in osteoarthritis therapy

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Окт. 4, 2023

Abstract Osteoarthritis (OA) is a prevalent joint disease that affects all the tissues within and currently lacks disease-modifying treatments in clinical practice. Despite potential of rapamycin for OA alleviation, its application hindered by challenge achieving therapeutic concentrations, which necessitates multiple injections per week. To address this issue, was loaded into poly(lactic-co-glycolic acid) nanoparticles (RNPs), are nontoxic, have high encapsulation efficiency exhibit sustained release properties treatment. The RNPs were found to promote chondrogenic differentiation ATDC5 cells prevent senescence caused oxidative stress primary mouse articular chondrocytes. Moreover, capable alleviate metabolism homeostatic imbalance chondrocytes both monolayer 3D cultures under inflammatory or stress. In destabilization medial meniscus (DMM) model, intra-articular injection effectively mitigated cartilage destruction, osteophyte formation, hypertrophy, synovial inflammation, pain. Our study demonstrates feasibility using as clinically translational therapy progression post-traumatic OA. Graphical abstract

Язык: Английский

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circNR3C2 promotes chondrogenic differentiation and cartilage repair of human adipose‐derived stem cells via the hsa‐miR‐647/SOX9 pathway

Animal Models and Experimental Medicine, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Human adipose-derived stem cells (hADSCs) are seed with application prospects in cartilage repair. However, the mechanism of hADSC chondrogenic differentiation is still unclear. This study identifies a novel circRNA, circNR3C2, which significantly upregulated during hADSCs. To analyze their role differentiation, hADSCs were separated and identified by flow cytometry. Thereafter, we conducted Alcian Blue staining to assess levels. Additionally, RT-qPCR was carried out detect levels cartilage-related genes COL2, Aggrecan SOX9. Moreover, overlapping target SOX9 circNR3C2 miRNAs detected bioinformatics luciferase analyses. Finally, confirmed vivo using animal models. We that cell surface receptors CD44, CD90 CD105 positively expressed on hADSCs, dramatically increased after 2 weeks. Expression COL2 also markedly increased. CircNR3C2 overexpression enhanced while up-regulating Aggrecan. Bioinformatics analysis hsa-miR-647 as miRNA Hsa-miR-647 can antagonize effect reverse its regulating expression Aggrecan, showed overexpressing promote repair vivo. show modulates hsa-miR-647-mediated differentiation; targeting may help develop new treatments manage disorders.

Язык: Английский

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Prg4-Expressing Chondroprogenitor Cells in the Superficial Zone of Articular Cartilage

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 5605 - 5605

Опубликована: Май 21, 2024

Joint-resident chondrogenic precursor cells have become a significant therapeutic option due to the lack of regenerative capacity in articular cartilage. Progenitor are located superficial zone cartilage, producing lubricin/Prg4 decrease friction cartilage surfaces during joint movement. Prg4-positive progenitors crucial maintaining joint’s structure and functionality. The disappearance progenitor leads changes hyaline over time, subchondral bone abnormalities, formation ectopic ossification. Genetic labeling cell technology has been main tool used characterize Prg4-expressing vivo through drug injection at different time points. This allows for determination origin tracking their progeny development damage. We endeavored highlight currently known information about Prg4-producing population underline significance role these its homeostasis. review focuses on joint, how they contribute postnatal formation, regeneration, consequences Prg4 deficiency cells. accumulated Prg4+ obtained various elegantly designed experiments using transgenic technologies identify potential opportunities further research.

Язык: Английский

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Advances in natural and synthetic macromolecules with stem cells and extracellular vesicles for orthopedic disease treatment

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 268, С. 131874 - 131874

Опубликована: Апрель 30, 2024

Язык: Английский

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Low‐density cultured cartilage cells expanded in platelet lysate present distinct features to develop an innovative clinical treatment for diffuse cartilage lesions

Knee Surgery Sports Traumatology Arthroscopy, Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

Abstract Purpose Chondrocyte‐based cell therapies are effective for the treatment of chondral lesions, but remain poorly indicated diffuse lesions in context early osteoarthritis (OA). The aim this study was to develop a protocol obtain chondroprogenitor cells suitable within OA. Methods Cartilage were expanded at low density human platelet lysate (hPL). A test performed exclude senescence. expression surface cluster differentiation 146, 166, major histocompatibility complex (MHC)‐I and MHC‐II genes interest evaluated, as well trophic potential these cells, by assessment lubricin matrix production. immunomodulatory assessed through their co‐culture with macrophages. Results hPL showed higher proliferation rate than standard‐density no replicative senescence, immunogenicity lubricin. Moreover, they presented an increased chondrogenic antihypertrophic markers, superior deposition if compared cultured standard density. induced on macrophages upregulation CD206, although increase CD163 observed presence low‐density cells. Conclusions These findings lay grounds explore clinical usefulness cartilage treat OA joints both autologous allogenic use. Level Evidence Not applicable.

Язык: Английский

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Insights into the Notch signaling pathway in degenerative musculoskeletal disorders: Mechanisms and perspectives

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 169, С. 115884 - 115884

Опубликована: Ноя. 17, 2023

Degenerative musculoskeletal disorders are a group of age-related diseases the locomotive system that severely affects patient's ability to work and cause adverse sequalae such as fractures even death. The incidence prevalence degenerative is rising owing aging world's population. Notch signaling pathway, which expressed in almost all organ systems, extensively regulates cell proliferation differentiation well cellular fate. shows increased activity retards progression degeneration some extent. review focuses on four major (osteoarthritis, intervertebral disc degeneration, osteoporosis, sarcopenia) summarizes pathophysiological functions these disorders, especially its role stem/progenitor cells each disorder. Finally, conclusion will be presented explore research application perspectives disorders.

Язык: Английский

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Silk Fibroin Films with Microscopic Surface Pattern as Promising Cartilage Tissue Engineering Scaffold in Supporting MSCs Amplification and Chondrogenic Differentiation

Journal of Natural Fibers, Год журнала: 2024, Номер 21(1)

Опубликована: Апрель 9, 2024

Mesenchymal stem cells (MSCs) with multipotential differentiation ability are main cell source for cartilage tissue engineering, but its application is restricted by the difficulty in regulating committed chondrogenic differentiation. Study revealed that certain substrate topographic structure can enhance of MSCs. This study prepared silk fibroin (SF) films gradient microscopic surface patterns based on P280-P7000 type specification sandpapers, and investigated adhesion, proliferation human adipose-derived (hADSCs) SF films. The results showed had convex dots diameter 3–50 μm, hADSCs could adhere proliferate well film. Transcriptomics analysis indicated tend to differentiate differently films, among which P1000 higher expression markers Sox-9 Col-II, activated yes associated protein (YAP) F-actin. In vitro co-culture OA knee seeded found satisfactory protection effect group. Overall, this preliminarily pattern may induce through extracellular matrix mediated Hippo/Yap pathway, provided a new strategy repair.

Язык: Английский

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The importance of the subchondral bone in the treatment of cartilage defects

Arthroskopie, Год журнала: 2024, Номер 37(3), С. 208 - 216

Опубликована: Апрель 22, 2024

Zusammenfassung Der hyaline Knorpel und der subchondrale Knochen bilden zusammen eine komplexe osteochondrale Einheit, die enge Kommunikation Abhängigkeit voneinander aufweist. Die Integrität des subchondralen Knochens ist sowohl im nativen Zustand als auch nach knorpelchirurgischen Eingriffen von entscheidender Bedeutung für den hyalinen Knorpel. Diese Gewebe befinden sich nicht nur in einem ständigen dynamischen biochemischen Austausch, sondern gleichzeitig Mikroarchitektur, dazu Lage ist, alltäglichen mechanischen Belastungen optimal zu absorbieren verteilen. Veränderungen beiden Gewebe, sei es oder Knochen, führen signifikanten Anpassungen auf jeweils anderen Seite. Daher unerlässlich, bei Bewertung Behandlung chondralen osteochondralen Defekten evaluieren ggf. chirurgisch behandeln, um ein optimales Behandlungsergebnis erzielen.

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Biomimetic-smart materials for osteochondral regeneration and repair

Microstructures, Год журнала: 2024, Номер 4(3)

Опубликована: Май 14, 2024

Osteochondral injuries represent prevalent clinical conditions with profound implications for functional impairment and diminished quality of life. Despite the considerable potential tissue engineering in osteochondral repair, substantial strides implementation remain elusive. Biomimetic materials, designed to emulate natural cartilage, offer a stabilized structure microenvironment capable accommodating diverse properties inherent different cartilage regions. Smart endowed ability deliver drugs, metal ions, growth factors contingent on disease progression, exert precise control over cellular epigenetic behaviors. This review scrutinizes nuanced characteristics both physiological pathological states. Subsequently, succinct overview recent applications biomaterials bionic smart attributes regeneration repair is provided. Finally, we propose our perspectives application biomimetic-smart materials emphasizing their translation.

Язык: Английский

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