Methods in cell biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Horticultural Plant Journal, Год журнала: 2022, Номер 8(4), С. 395 - 407
Опубликована: Апрель 29, 2022
Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) system has recently become one popular technology due to its efficiency, precision, and simplicity compared with other genome editing tools such as Zinc Finger Nucleases (ZFNs) Transcription Activator Like Effector (TALENs). Horticultural crops provide energy health-keeping nutrients humankind. Genome-editing widely adopted in horticultural breeding the increasing demand for high yield better-quality crops. Here, we describe CRISPR/Cas9 construction, optimization, including sgRNA promoter, design, Cas9 protein SpCas9 variants orthologs, vector delivery methods. We also summarized application of this plants stress responses enhancement, fruit quality improvement, cultivation traits modification. This detailed review was compiled help establish comprehensive understanding systems a reference further developing manipulate plant effectively.
Язык: Английский
Процитировано
29
Frontiers in Genetics, Год журнала: 2022, Номер 13
Опубликована: Июнь 28, 2022
Legumes are rich in protein and phytochemicals have provided a healthy diet for human beings thousands of years. In recognition the important role they play nutrition agricultural production, researchers made great efforts to gain new genetic traits legumes such as yield, stress tolerance, nutritional quality. recent years, significant increase genomic resources legume plants has prepared groundwork applying cutting-edge breeding technologies, transgenic genome editing, selection crop improvement. addition different editing technologies including CRISPR/Cas9-based system, this review article discusses advances plant-specific gene-editing methods, well problems potential benefits associated with improvement crops agronomic properties. The been effectively used model like alfalfa lotus, soybean, cowpea, chickpea. We also discussed methods improvements recalcitrant legumes. Despite immense opportunities can offer legumes, governmental regulatory restrictions present major concern. context, comparison framework strategies European Union United States America was discussed. Gene-editing opened up possibilities breeding.
Язык: Английский
Процитировано
29
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(17), С. 10175 - 10175
Опубликована: Сен. 5, 2022
CRISPR/Cas-based genome editing technology provides straightforward, proficient, and multifunctional ways for the site-directed modification of organism genomes genes. The application CRISPR-based in plants has a vast potential value gene function research, germplasm innovation, genetic improvement. complexity woody may pose significant challenges expansion various new techniques, such as Cas9, 12, 13, 14 effectors, base editing, particularly timberland species with long life span, huge genome, ploidy. Therefore, many novel optimisms have been drawn to molecular breeding research based on plants. This review summarizes recent development CRISPR/Cas applications essential traits, including wood properties, flowering, biological stress, abiotic growth, We outlined current problems future trends this improvement products
Язык: Английский
Процитировано
28
Plant Stress, Год журнала: 2024, Номер 11, С. 100409 - 100409
Опубликована: Фев. 19, 2024
Abiotic stressors such as drought, low temperature, heavy metals, waterlogging, nutrient imbalance, and salinity are major factors that affect the growth development of crop plants, which, in turn, results severe loss production yield economically important crops. Current literature backs up effect high on almost all plants. Thus, it can be concluded stress is amongst most dominant abiotic current farming systems, which counteract achieving goal "zero hunger." Consequently, there a dire need to improve plants develop tolerance for higher production, even agricultural habitats. The last few decades have established mechanistic understanding identified molecular determinants favoring Stress-responsive transcriptional control best strategy adapt alleviate stressors, especially stress. In transcription (TFs) central regulation include bZIP, WRKY, NAC, AP2/ERFBP, MYB. Studying these TFs their mechanisms facilitate modification at genetic level modify tolerance. Collectively, reports suggest enhance directly or indirectly through diverse signaling pathways. This review summarizes recent developments deciphering controlling cellular process gene expression under Finally, we highlight way forward applying genome editing technologies modulate hallmark genes circumventing
Язык: Английский
Процитировано
7
Analytica Chimica Acta, Год журнала: 2024, Номер 1303, С. 342519 - 342519
Опубликована: Март 26, 2024
Язык: Английский
Процитировано
6
Journal of Cellular Physiology, Год журнала: 2020, Номер 236(3), С. 1996 - 2007
Опубликована: Авг. 25, 2020
Abstract Seed size and number are central to the evolutionary fitness of plants also crucial for seed production crops. However, molecular mechanisms control poorly understood in Brassica Here, we report gene cloning, expression analysis, functional characterization EOD3/CYP78A6 rapeseed. BnaEOD3 has four copies located two subgenomes, which exhibited a steady higher during development with differential among copies. The targeted mutations were efficiently generated by stable transformation CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat) vector. These stably transmitted T 1 2 generations large collection homozygous mutants combined loss‐of‐function alleles across created phenotyping. All mutant lines had shorter siliques, smaller seeds, an increased seeds per silique, quadrable showed most significant changes these traits. Consequently, weight plant 13.9% on average compared that wild type, indicating have redundant functions phenotypes different allelic combinations revealed differentiation subgenomes. Cytological observations indicated could act maternally promote cotyledon cell expansion proliferation regulate growth Collectively, our findings reveal quantitative involvement function development, but provided valuable resources rapeseed breeding programs.
Язык: Английский
Процитировано
44
International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(11), С. 5585 - 5585
Опубликована: Май 25, 2021
A world with zero hunger is possible only through a sustainable increase in food production and distribution the elimination of poverty. Scientific, logistical, humanitarian approaches must be employed simultaneously to ensure security, starting farmers breeders extending policy makers governments. The current agricultural system facing challenge sustainably increasing grain quality yield enhancing resistance biotic abiotic stress under intensifying pressure climate change. Under present circumstances, conventional breeding techniques are not sufficient. Innovation plant critical managing challenges achieving crop production. Novel techniques, involving series developments from genome editing speed integration omics technology, offer relevant, versatile, cost-effective, less time-consuming ways precision breeding. Opportunities edit agriculturally significant genes now exist as result new techniques. These range random (physical chemical mutagens) non-random meganucleases (MegaN), zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9), CRISPR Prevotella Francisella1 (Cpf1), base (BE), prime (PE). Genome that promote improvement hybrid seed production, induced apomixis, prioritized when selecting for genetic gain restricted timeframe. novel CRISPR-associated variants, namely BE PE, can generate transgene-free plants more frequency therefore being used knocking out interest. We provide comprehensive review evolution technologies, especially application third-generation technologies achieve various objectives within regulatory regimes adopted by countries. Future development optimization forward reverse genetics security evaluated.
Язык: Английский
Процитировано
38
The Crop Journal, Год журнала: 2021, Номер 10(2), С. 287 - 299
Опубликована: Июль 15, 2021
Gibberellic acid (GA), a ubiquitous phytohormone, has various effects on regulators of plant growth and development. GAs promote by overcoming restraint mediated DELLA proteins (DELLAs). DELLAs, in the GRAS family plant-specific nuclear proteins, are transcriptional harboring unique N-terminal GA perception region for binding receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1) C-terminal domain necessary repression activity via interaction with multiple regulatory proteins. The conserved DELLAs evolved to form mode GID1/DELLA-mediated signaling originating bryophytes ferns. Binding GID1 increases affinity between SCF E3 ubiquitin–ligase complex, thus promoting eventual destruction 26S proteasome. negatively regulate response releasing transcription factors directly activate downstream genes indirectly biosynthesis increasing responsiveness feedback control transcription. communicates extensively other hormones uses crosstalk In this review, we summarize current understanding evolutionary DELLA-mediated gibberellin functional diversification DELLA, focusing primarily interactions diverse phytohormones.
Язык: Английский
Процитировано
35
Plants, Год журнала: 2021, Номер 10(9), С. 1914 - 1914
Опубликована: Сен. 14, 2021
With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet increasing demand food. Classical involve, however, a backbreaking process that cannot equipoise with demand. RNA-based i.e., RNAi-mediated gene regulation and site-specific nuclease-based CRISPR/Cas9 system editing has made advances in efficient targeted modification many crops higher yield resistance diseases different stresses. In functional genomics, RNA interference (RNAi) propitious regulatory approach plays significant role by permitting downregulation of expression small molecules interfering without affecting other genes. Gene technologies viz. clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as powerful tool precise nearly all crops’ genome sequences generate variation accelerate breeding efforts. this regard, review highlights diverse roles applications RNAi improve agronomically important plants enhance yields increase tolerance environmental stress (biotic or abiotic). Ultimately, these can prove be view global food security sustainable agriculture.
Язык: Английский
Процитировано
34
Food Control, Год журнала: 2022, Номер 141, С. 109193 - 109193
Опубликована: Июнь 17, 2022
Язык: Английский
Процитировано
26