Advances in stem cells and their niches, Год журнала: 2023, Номер unknown, С. 59 - 90
Опубликована: Янв. 1, 2023
Язык: Английский
Biomolecules, Год журнала: 2024, Номер 14(4), С. 459 - 459
Опубликована: Апрель 9, 2024
The skin is the outer layer of human body, and it crucial in defending against injuries damage. regenerative capacity aging damaged caused by exposure to external stimuli significantly impaired. Currently, rise average life expectancy modern population’s aesthetic standards have sparked a desire for stem-cell-based therapies that can address health conditions. In recent years, mesenchymal stem cells (MSCs) as therapeutic agents provided promising effective alternative managing regeneration rejuvenation, attributing their healing capacities be applied aged skin. However, has been established effects MSC may primarily mediated paracrine mechanisms, particularly release exosomes (Exos). Exosomes are nanoscale extracellular vesicles (EVs) lipid bilayer membrane structures naturally released different types cells. They influence physiological pathological processes recipient transferring variety bioactive molecules, including lipids, proteins, nucleic acids such messenger RNAs (mRNAs) microRNAs (miRNAs) between cells, thus playing an important role intercellular communication activating signaling pathways target Among them, miRNAs, type endogenous regulatory non-coding RNA, often incorporated into molecules regulating protein biosynthesis. Emerging evidence suggests exosomal miRNAs from play key rejuvenation targeting multiple genes various biological processes, participating inflammatory responses, cell migration, proliferation, apoptosis. this review, we summarize studies observations on how MSC-derived contribute tissue, with particular attention applications bioengineering methods manipulating miRNA content exosome cargo improve potential. This review provide new clues diagnosis treatment damage aging, well assist investigators exploring innovative strategies treating multitude problems aim delaying promoting regeneration, maintaining healthy
Язык: Английский
Процитировано
9
Clinical Cosmetic and Investigational Dermatology, Год журнала: 2024, Номер Volume 17, С. 1603 - 1612
Опубликована: Июль 1, 2024
Abstract: Alopecia is considered a widespread yet troubling health issue, with limited treatment options. As membranous structures derived from cells carrying proteins, nucleic acids and lipids, exosomes functionally medicate intercellular communication alter the responses of recipient cells, resulting in disease restraint or promotion. Exosomes have broad prospects diagnosis diseases. Studies using animal models at cellular level clearly shown that several types including dermal papilla mesenchymal stem notable capacity to promote hair growth, suggesting may provide new option treat alopecia. Here, we present thorough review most recent progress application growth. Keywords: exosome, follicle, alopecia
Язык: Английский
Процитировано
6
Cell Biochemistry and Biophysics, Год журнала: 2025, Номер unknown
Опубликована: Фев. 27, 2025
Язык: Английский
Процитировано
0
Journal of Cosmetic Dermatology, Год журнала: 2023, Номер 22(11), С. 2996 - 3007
Опубликована: Авг. 8, 2023
Androgenetic alopecia (AGA) is the most common cause of chronic progressive hair loss in men, and AGA has a severe negative impact on quality life physical mental health patients.Four female C57BL/6 mice were isolated from DP cells culture (≤4 generations) after stimulation DPC proliferation by herbal concentrations obtained CCK-8 method, exosomes differential centrifugation at low temperature. Testosterone propionate topical removal treatments used together to establish mouse model, which was treated with LTF, 5% minoxidil, LTF-DPC-EXO, respectively. ELISA detect serum hormone levels, vivo tracing observe dynamic changes exosomes, H&E staining showed follicle tissue, (q) RT-PCR WB dorsal skin VEGF, AKT1, CASP3 expression tissues.Hair regeneration significant LTF group, minoxidil LTF-DPC-EXO group mice, growth only seen local model group. The T all treatment groups lower than that e2 higher western blot VEGF AKT1 expressions upregulated Caspase3 downregulated sections groups.DPC-EXO through may activate PI3K/AKT signaling pathway inhibit CASP3, thereby protecting restore growth.
Язык: Английский
Процитировано
12
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139292 - 139292
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Pharmaceutics, Год журнала: 2025, Номер 17(2), С. 147 - 147
Опубликована: Янв. 22, 2025
Exosomes have emerged as promising therapeutic agents in regenerative medicine. This review introduces a novel cell type-oriented perspective to systematically analyze exosomal properties therapies. To our knowledge, this is the first comprehensively compare exosomes based on cellular source type, offering unprecedented insights into selecting optimal exosome producers for targeted applications. Factors beyond origin influencing efficacy, such donor sites and collection methods, are also explored here. By synthesizing key advances, we propose research directions end. We aim accelerate development of more effective exosome-based therapies highlight underexplored rapidly evolving field.
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 29, 2025
Hypertrophic scar (HS) is a common fibroproliferative disorders with no fully effective treatments. The conversion of fibroblasts to myofibroblasts known play critical role in HS formation, making it essential identify molecules that promote myofibroblast dedifferentiation and elucidate their underlying mechanisms. In this study, we used comparative transcriptomics single-cell sequencing key pathways mediate fibrosis transdifferentiation. Epidermal stem cell-derived extracellular vesicles (EpiSC-EVs) were isolated via ultracentrifugation filtration, followed by miRNA miRNAs targeting molecules. After vitro vivo treatment EpiSC-EVs, assessed antifibrotic effects through scratch assays, collagen contraction Western blotting, immunofluorescence. Transcriptomic rescue experiments investigate the molecular mechanism which miR-203a-3p EpiSC-EVs induces dedifferentiation. Our results indicate PIK3CA overexpressed tissues positively correlates fibrosis. absorbed scar-derived fibroblasts, promoting from quiescent fibroblasts. Mechanistically, plays an inhibiting expression PI3K/AKT/mTOR pathway hyperactivation, thereby reducing formation. studies confirmed attenuate excessive scarring miR-203a-3p/PIK3CA axis, suggesting as promising therapeutic approach for HS.
Язык: Английский
Процитировано
0
Experimental Cell Research, Год журнала: 2025, Номер unknown, С. 114483 - 114483
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Heliyon, Год журнала: 2024, Номер 10(4), С. e26204 - e26204
Опубликована: Фев. 1, 2024
BackgroundAndrogenetic alopecia (AGA) is the most common form of hair loss. Studies have suggested a potential link to metabolic disorders, but with conflicting results. To elucidate lipidomics profile and sex-specific variations in AGA, while exploring correlation between AGA syndrome (MetS).MethodsThe patients (n = 83) healthy controls 84) were collected study. The lipid profiles analyzed using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Serum levels important factors associated namely dihydrotestosterone (DHT), prostaglandin D2 (PGD2) transforming growth factor-β1 (TGF-β1) quantified ELISA.ResultsCompared controls, had higher probability MetS (26.51% vs 11.9%, P < 0.05). Fifty-one differentially expressed lipids identified AGA. kind triglyceride (TG) significantly increased, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserine (PS) exhibited remarkable decrease. PC (16:2/21:6), (34:4p), PE (41:7), (44:12), PG (40:9), PI (32:2) TG (15:0/18:1/18:1) as biomarkers highest specificity. DHT, PGD2 TGF-β1 elevated All seven showed significant correlations (34:4p) PGD2, displayed exclusively (all Furthermore, these also linked systolic blood pressure BMI, some them associations total cholesterol HDL-C. In subgroups, forty-two male control eighty-one female control. (16:2/21:6) was only specific both sexes.ConclusionsAberrant metabolism observed distinct lipidomic closely related MetS-related indicators. It provides foundation for revealing mechanisms
Язык: Английский
Процитировано
3
International Journal of Biological Sciences, Год журнала: 2023, Номер 19(14), С. 4588 - 4607
Опубликована: Янв. 1, 2023
Hair graying is a common and visible sign of aging resulting from decreased or absence melanogenesis.Although it has been established that gray hair greatly impacts people's mental health social life, there no effective countermeasure other than dyes.It long thought reversal on large scale rare.However, recent study reported individual darkening phenomenon, suggesting the possibility large-scale hair.In this article, we summarize regulation mechanism melanogenesis review existing cases repigmentation caused by several factors, including monoclonal antibodies drugs, tyrosine kinase inhibitors (TKIs), immunomodulators, micro-injury, tumors, speculate mechanisms behind them.This offers some insights for further research into modulation presents novel perspective development clinical therapies, with emphasis topical treatments.
Язык: Английский
Процитировано
8