Journal of Muscle Research and Cell Motility, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 30, 2024
Язык: Английский
Nature Reviews Cardiology, Год журнала: 2024, Номер 21(6), С. 361 - 378
Опубликована: Янв. 2, 2024
Язык: Английский
Процитировано
14
Ageing Research Reviews, Год журнала: 2024, Номер 102, С. 102576 - 102576
Опубликована: Ноя. 7, 2024
Язык: Английский
Процитировано
5
Biomolecules, Год журнала: 2025, Номер 15(2), С. 310 - 310
Опубликована: Фев. 19, 2025
In mammals, because cardiomyocytes withdraw from cell-cycle activities shortly after birth, the heart cannot repair damage caused by a myocardial injury; thus, understanding how proliferate is among most important topics in cardiovascular sciences. newborn neonatal when left ventricular injury applied hearts earlier than postnatal day 7, actively and regenerate lost myocardium following weeks. The regulators promoting cardiomyocyte proliferation were discovered analyzing transcriptomic data generated models. Most of these support mRNA production machinery, yet requires translation into functional proteins under regulation RNA-binding (RBPs). this work, we performed meta-analysis to study relationship between RBP expression proliferation. To identify RBPs associated with mouse pig proliferation, single-nuclei RNA sequencing (snRNA-seq) regenerating reanalyzed via an Autoencoder focusing on expression. We also analyzed new bulk RNA-seq two human-induced pluripotent stem cell-derived (hiPSC) (hiPSC-CM) cell lines; first line was harvested sixteen days differentiation, cells still proliferated, second one hundred forty ceased cycle activity. Then, mouse, pig, hiPSC-CM compared across species. Twenty-one found be consistently upregulated, six downregulated proliferating hiPSC-derived cardiomyocytes. Among upregulated species, immunofluorescence-based imaging analysis validated significant increase DHX9, PTBP3, HNRNPUL1, DDX6 CMs. This all species demonstrated strong
Язык: Английский
Процитировано
0
American Journal Of Pathology, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Journal of Cardiovascular Development and Disease, Год журнала: 2024, Номер 11(3), С. 88 - 88
Опубликована: Март 5, 2024
The post-transcriptional regulation of gene expression plays an important role in heart development and disease. Cardiac-specific alternative splicing, mediated by RNA-binding proteins, orchestrates the isoform switching proteins that are essential for cardiomyocyte organization contraction. Dysfunctions impair cause main types cardiomyopathies, which represent a heterogenous group abnormalities severely affect structure function. In particular, mutations RBM20 RBFOX2 associated with dilated cardiomyopathy, hypertrophic or hypoplastic left syndrome. Functional analyses different animal models also suggest possible roles other cardiomyopathies because their involvement organizing cardiac programming. Recent studies have provided significant insights into causal relationship between cardiovascular diseases. They show potential correcting pathogenic to rescue cardiomyopathy promote regeneration. Therefore, emerged as promising targets therapeutic interventions dysfunction. challenge remains decipher how they coordinately regulate temporal spatial target genes ensure function homeostasis. This review discusses recent advances understanding implications several well-characterized aim identifying research gaps further investigation this field.
Язык: Английский
Процитировано
3
Ageing Research Reviews, Год журнала: 2024, Номер 100, С. 102413 - 102413
Опубликована: Июль 19, 2024
Язык: Английский
Процитировано
3
Biomedicines, Год журнала: 2024, Номер 12(2), С. 340 - 340
Опубликована: Фев. 1, 2024
RNA binding proteins (RBPs) post-transcriptionally regulate gene expression by associating with regulatory sequences in the untranslated regions of mRNAs. Cold-inducible RBP (CIRP) is a stress-induced that was recently shown to modulate inflammation response cellular stress, where it increases or decreases pro-tumorigenic (proinflammatory) cytokines different contexts. CIRP altered several cancers, including breast cancer, but effects on cancer not known. Here, we investigate if alters growth and inflammatory profile tumors. Transgenic mice overexpressing mammary epithelium were crossed PyMT mouse model both early late tumorigenesis assessed. The knockdown also assessed Py2T cell grafts. Overexpression led decreased model. Conversely, grafts increased tumor growth. Luminex cytokine assays environment. CIRP/PyMT glands/mammary tumors serum had promote inflammation, angiogenesis, metastasis compared glands serum, documenting shift towards an environment less supportive tumorigenesis. overexpression CD4+ helper T cells CD8+ cytotoxic Overall, these data support role for as potent antitumor molecule suppresses local systemic inflammation.
Язык: Английский
Процитировано
1
Non-coding RNA Research, Год журнала: 2024, Номер 9(3), С. 744 - 758
Опубликована: Фев. 22, 2024
Trastuzumab-induced cardiotoxicity (TIC) is a common and serious disease with abnormal cardiac function. Accumulating evidence has indicated some non-coding RNAs (ncRNAs), acting as competing endogenous (ceRNAs), have been reported to affect the cardiovascular process. However, role of ncRNA-mediated ceRNA regulatory mechanisms in TIC remains unclear. The present research aims systematically investigate alterations expressions all ncRNA using whole-transcriptome RNA sequencing. sequencing revealed total 43 circular (circRNA), 270 long noncoding (lncRNA), 12 microRNA (miRNA), 4131 mRNA were aberrantly expressed mice heart treated trastuzumab. A circRNA-based network 82 nodes 91 edges lncRNA-based 111 112 established, core genes, miR-31–5p miR-644–5p, screened plug-in CytoNCA, which potential roles treatment TIC. Moreover, KEGG GO analyses performed for genes ceRNAs networks. predicted bioinformatics analysis ncRNAs TIC, would assist understanding mechanism finding effective prevention targets.
Язык: Английский
Процитировано
1
Theranostics, Год журнала: 2024, Номер 14(11), С. 4499 - 4518
Опубликована: Янв. 1, 2024
Adult neurogenesis in the subventricular zone (SVZ) is essential for maintaining neural homeostasis, and its dysregulation contributes to anosmia delayed tissue healing neurological disorders, such as Parkinson's disease (PD). Despite intricate regulatory networks identified SVZ neurogenesis, molecular mechanisms dynamically stem/progenitor cells (NSPCs) response physiological pathological stimuli remain incompletely elucidated.
Язык: Английский
Процитировано
1
Biomolecules, Год журнала: 2024, Номер 14(9), С. 1056 - 1056
Опубликована: Авг. 25, 2024
RNA-binding proteins (RBPs) have pivotal roles in cardiovascular biology, influencing various molecular mechanisms underlying diseases (CVDs). This review explores the significant of RBPs, focusing on their regulation RNA alternative splicing, polyadenylation, and editing, impact CVD pathogenesis. For instance, RBPs are crucial myocardial injury, contributing to disease progression repair mechanisms. systematically analyzes arrhythmias, infarction, heart failure, revealing intricate interactions that influence outcomes. Furthermore, potential as therapeutic targets for dysfunction is explored, highlighting advances drug development clinical research. also discusses emerging role biomarkers diseases, offering insights into diagnostic prognostic potential. Despite progress, current research faces several limitations, which critically examined. Finally, this identifies major challenges outlines future directions advance understanding application medicine.
Язык: Английский
Процитировано
1