Laccase: A Green Biocatalyst Offers Immense Potential for Food Industrial and Biotechnological Applications
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(44), С. 24158 - 24169
Опубликована: Окт. 22, 2024
Laccase,
a
multipurpose
biocatalyst,
is
widely
distributed
across
all
kingdoms
of
life
and
plays
key
role
in
essential
biological
processes
such
as
lignin
synthesis,
degradation,
pigment
formation.
These
functions
are
critical
for
fungal
growth,
plant-pathogen
interactions,
maintenance
soil
health.
Due
to
its
broad
substrate
specificity,
multifunctional
nature,
environmentally
friendly
characteristics,
laccase
employed
catalyst
various
green
chemistry
initiatives.
With
ability
oxidize
diverse
range
phenolic
nonphenolic
compounds,
has
also
been
found
be
useful
food
additive
assessing
quality
parameters.
Ongoing
advancements
research
technology
continually
expanding
the
recognition
laccase's
potential
address
global
environmental,
health,
energy
challenges.
This
review
aims
provide
insights
into
applications
laccases
biotechnology
industry.
Язык: Английский
Integrated Multi-Omics Analysis to Investigate the Molecular Mechanisms Underlying the Response of Auricularia heimuer to High-Temperature Stress
Journal of Fungi,
Год журнала:
2025,
Номер
11(3), С. 167 - 167
Опубликована: Фев. 20, 2025
High-temperature
stress
is
a
key
factor
that
reduces
the
yields
of
edible
fungi.
Auricularia
heimuer
(A.
heimuer)
nutrient-rich
fungus
widely
cultivated
in
China.
In
this
study,
we
analyzed
physiological,
transcriptomic,
and
metabolomic
results
A.
(variety
“Hei29”)
under
high-temperature
stress.
Our
findings
revealed
high
temperatures
(30
°C
35
°C)
significantly
reduced
hyphal
growth,
increased
malondialdehyde
content
antioxidant
enzyme
activity,
enhanced
accumulation
secondary
metabolites,
such
as
phenolic
compounds
flavonoids.
A
total
15
candidate
genes
potentially
responsive
to
were
identified
through
transcriptomic
analysis,
including
those
involved
regulating
defense,
heat
shock
response,
sugar
metabolism,
amino
acid
accumulating
metabolites.
Metabolomic
analysis
three
metabolites
stress,
kinetin,
flavonoids,
caffeic
acid,
well
several
metabolic
pathways,
nucleotide
ABC
transporters,
cofactor
biosynthesis.
These
mechanisms
help
mitigate
oxidative
damage
cellular
structures
energy
deficits
caused
by
elevated
temperatures,
enabling
maintain
stability,
function,
growth
This
study
first
explore
molecular
mechanism
response
The
provide
valuable
insights
into
tolerance
heimuer,
highlighting
potential
targets
for
developing
heat-tolerant
strains
industrial
application.
Язык: Английский
Gene Editing in Ganoderma lucidum: Development, Challenges, and Future Prospects
Journal of Fungi,
Год журнала:
2025,
Номер
11(4), С. 310 - 310
Опубликована: Апрель 14, 2025
As
an
emerging
and
innovative
technology,
gene-editing
technology
has
been
widely
applied
in
crop
breeding,
human
disease
treatment,
animal
model
research,
drug
vaccine
development,
microbial
engineering.
We
mainly
introduce
the
development
of
application
clustered
regularly
interspaced
short
palindromic
repeat/Cas9
(CRISPR/Cas9)
Ganoderma
lucidum
current
challenges
optimization
strategies
use
as
well
status
breeding.
Finally,
future
research
directions
that
gene
editing
may
explore
breeding
are
prospects
given
existing
background,
directions,
prospects.
Язык: Английский
Microbial Community Response and Assembly Process of Yellow Sand Matrix in a Desert Marginal Zone Under Morchella Cultivation
Microorganisms,
Год журнала:
2025,
Номер
13(4), С. 921 - 921
Опубликована: Апрель 16, 2025
In
this
study,
we
investigated
the
adaptation
of
yellow-sand-substrate
Morchella
cultivation
in
desert
fringe
and
its
effect
on
soil
physicochemical
properties
microbial
communities.
The
qPCR
high-throughput
sequencing
with
null
modeling
analyzed
diversity,
networks,
assembly
under
nutrient
supplementation,
linking
changes
to
dynamics.
results
showed
that
yellow
sand
substrate
can
be
planted
area,
as
bags
resulted
a
yield
691
g/m2
fruit
units.
Cultivation
could
significantly
increase
substrate,
such
organic
matter
(SOM),
total
nitrogen
(TN),
ammonium
(NH4+−N),
amount
carbon
(MBC/MBN).
fungal
community
was
dominated
by
Ascomycota,
Basidiomycota,
Firmicutes,
Bacteroidota,
Actinobacteriota.
RDA
analysis
Ascomycota
Proteobacteria
were
positively
correlated
NH4+−N,
MBN,
SOM,
MBC,
acting
potassium
(AK),
TN,
C/N.
promoted
positive
correlation-dominant
network
pattern
substrate.
bag
treatment
reduced
bacterial
complexity
while
enhancing
complexity,
connectivity
stability,
accompanied
significant
increases
Proteobacteria,
Cladosporium,
Thermomyces
relative
abundances
during
until
original
degradation.
Deterministic
processes
communities,
morel
drove
toward
heterogeneous
selection
processes.
study
revealed
economic
value
application
potential
improving
sandy
soil,
which
is
great
importance
for
practical
mushrooms
desert.
Язык: Английский
Plant Secondary Metabolites—Central Regulators Against Abiotic and Biotic Stresses
Metabolites,
Год журнала:
2025,
Номер
15(4), С. 276 - 276
Опубликована: Апрель 16, 2025
As
global
climates
shift,
plants
are
increasingly
exposed
to
biotic
and
abiotic
stresses
that
adversely
affect
their
growth
development,
ultimately
reducing
agricultural
productivity.
To
counter
these
stresses,
produce
secondary
metabolites
(SMs),
which
critical
biochemical
essential
compounds
serve
as
primary
defense
mechanisms.
These
diverse
compounds,
such
alkaloids,
flavonoids,
phenolic
nitrogen/sulfur-containing
act
natural
protectants
against
herbivores,
pathogens,
oxidative
stress.
Despite
the
well-documented
protective
roles
of
SMs,
precise
mechanisms
by
environmental
factors
modulate
accumulation
under
different
stress
conditions
not
fully
understood.
This
review
provides
comprehensive
insights
into
recent
advances
in
understanding
functions
SMs
plant
emphasizing
regulatory
networks
biosynthetic
pathways.
Furthermore,
we
explored
unique
contributions
individual
SM
classes
responses
while
integrating
findings
across
entire
spectrum
diversity,
providing
a
resilience
multiple
conditions.
Finally,
highlight
emerging
strategies
for
harnessing
improve
crop
through
genetic
engineering
present
novel
solutions
enhance
sustainability
changing
climate.
Язык: Английский
Genome editing tools based improved applications in macrofungi
Molecular Biology Reports,
Год журнала:
2024,
Номер
51(1)
Опубликована: Июль 30, 2024
Язык: Английский
Biological Characteristics of the Mycelium and Optimization of the Culture Medium for Phallus dongsun
Polish Journal of Microbiology,
Год журнала:
2024,
Номер
73(2), С. 237 - 252
Опубликована: Июнь 1, 2024
Abstract
This
study
aimed
to
elucidate
the
influence
of
various
culture
medium
components,
including
carbon
sources,
nitrogen
inorganic
salts,
suspension
agents,
and
temperature,
on
mycelial
growth
characteristics
Phallus
dongsun
.
Employing
single-factor
experiments
response
surface
methodology
within
glass
Petri
dishes,
research
identified
that
carrot
powder,
soybean
ZnSO
4
notably
enhanced
proliferation
aerial
mycelium,
significantly
augmenting
rate
P.
mycelium.
The
resultant
mycelium
was
observed
be
dense,
robust,
fluffy
in
texture.
In
particular,
markedly
accelerated
rate.
Furthermore,
xanthan
gum
found
effectively
modulate
medium’s
viscosity,
ensuring
a
stable
facilitating
nutrient
equilibrium.
optimal
cultivation
temperature
determined
25°C,
with
ceasing
below
5°C
perishing
at
temperatures
exceeding
35°C.
composition
established
as
follows:
wheat
starch
5
g/l,
powder
7.50
glucose
10
0.71
NH
Cl
0.68
0.5
agar
20
g/l.
Under
these
optimized
conditions,
exhibited
rapid
(1.04
±
0.14
mm/day),
characterized
by
thick,
well-developed
structure.
investigation
provides
theoretical
foundation
for
conservation,
strain
selection,
breeding
Язык: Английский
Diversity of Host Species and Optimized Cultivation Practices for Enhanced Bioactive Compound Production in Cordyceps militaris
Applied Sciences,
Год журнала:
2024,
Номер
14(18), С. 8418 - 8418
Опубликована: Сен. 19, 2024
Cordyceps
militaris,
recognized
for
its
diverse
and
potent
medicinal
properties,
plays
a
critical
role
in
herbal
medicine.
This
study
investigates
the
genus
Cordyceps,
particularly
focusing
on
C.
species
widely
utilized
East
Asian
traditional
medicine
therapeutic
properties.
militaris
is
rich
bioactive
compounds,
including
cordycepin,
adenosine,
polysaccharides,
ergosterol,
mannitol,
which
contribute
to
broad
physiological
activities.
The
research
aims
explore
diversity
of
host
cultivation
assess
their
impact
biological
activity
chemical
composition
fungus.
highlights
variability
efficacy
compounds
due
different
conditions
species,
emphasizing
need
standardized
practices.
Advances
genetic
engineering
fermentation
technology
have
significantly
enhanced
production
these
metabolites,
supporting
sustainable
scale-up
cultivation.
Future
should
continue
molecular
mechanisms
identify
new
clinical
applications,
improve
efficiency
environmental
sustainability.
comprehensive
review
provides
insights
researchers,
healthcare
professionals,
cultivators
optimizing
commercial
applications.
Язык: Английский
Whole-Genome Sequence Analysis of Flammulina filiformis and Functional Validation of Gad, a Key Gene for γ-Aminobutyric Acid Synthesis
Journal of Fungi,
Год журнала:
2024,
Номер
10(12), С. 862 - 862
Опубликована: Дек. 12, 2024
Flammulina
filiformis
is
one
of
the
widely
produced
edible
fungi
worldwide.
It
rich
in
γ-aminobutyric
acid
(GABA),
a
non-protein
amino
with
important
physiological
functions
humans.
To
investigate
key
genes
GABA
metabolic
pathway
F.
filiformis,
we
isolated
monokaryon
Fv-HL23-1
from
factory-cultivated
strain
Fv-HL23
and
then
sequenced
assembled
genome
using
PacBio
Sequel
Illumina
NovaSeq
sequencing
platforms.
The
results
showed
that
comprised
140
scaffolds
total
length
40.96
Mb,
GC
content
49.62%,
an
N50
917,125
bp,
14,256
protein-coding
genes.
Phylogenetic
analysis
based
on
whole
revealed
close
evolutionary
relationship
Armillaria
mellea,
Lentinula
edodes,
Schizophyllum
commune.
A
589
carbohydrate-active
enzymes
were
identified
Fv-HL23-1,
suggesting
its
strong
lignocellulose
degradation
ability,
108
CYP450
gene
family
members
identified,
such
as
resistance
to
stress,
secondary
metabolite
synthesis,
growth
development.
proteins
glutamate
decarboxylase
1
(Ff-GAD1)
2
(Ff-GAD2),
which
may
be
responsible
for
by
protein
alignment.
Molecular
docking
Ff-GAD2
have
better
catalytic
activity
than
Ff-GAD1.
verify
function
Ff-gad2,
heterologous
expression
mycelia
mononuclear
Hypsizigus
marmoreus
was
analyzed.
Compared
wild
type,
increased
85.40–283.90%,
rate
9.39
±
2.35%,
fresh
weight
18.44
7.57%.
play
role
synthesis.
In
addition,
full-length
Ff-gad2
7.96
1.39
times
compared
exon
level
H.
mycelia,
intron
contribute
Ff-GAD2.
Based
whole-genome
sequencing,
analyzed
enzyme
system
related
life
activities
focusing
Ff-GAD,
synthesis
pathway.
lay
foundation
elucidating
metabolism
developing
targeted
breeding
strategies
GABA-producing
fungi.
Язык: Английский