Whole-Genome Sequence Analysis of Flammulina filiformis and Functional Validation of Gad, a Key Gene for γ-Aminobutyric Acid Synthesis DOI Creative Commons
Wenyun Li, Junjun Shang, Dapeng Bao

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 862 - 862

Published: Dec. 12, 2024

Flammulina filiformis is one of the widely produced edible fungi worldwide. It rich in γ-aminobutyric acid (GABA), a non-protein amino with important physiological functions humans. To investigate key genes GABA metabolic pathway F. filiformis, we isolated monokaryon Fv-HL23-1 from factory-cultivated strain Fv-HL23 and then sequenced assembled genome using PacBio Sequel Illumina NovaSeq sequencing platforms. The results showed that comprised 140 scaffolds total length 40.96 Mb, GC content 49.62%, an N50 917,125 bp, 14,256 protein-coding genes. Phylogenetic analysis based on whole revealed close evolutionary relationship Armillaria mellea, Lentinula edodes, Schizophyllum commune. A 589 carbohydrate-active enzymes were identified Fv-HL23-1, suggesting its strong lignocellulose degradation ability, 108 CYP450 gene family members identified, such as resistance to stress, secondary metabolite synthesis, growth development. proteins glutamate decarboxylase 1 (Ff-GAD1) 2 (Ff-GAD2), which may be responsible for by protein alignment. Molecular docking Ff-GAD2 have better catalytic activity than Ff-GAD1. verify function Ff-gad2, heterologous expression mycelia mononuclear Hypsizigus marmoreus was analyzed. Compared wild type, increased 85.40–283.90%, rate 9.39 ± 2.35%, fresh weight 18.44 7.57%. play role synthesis. In addition, full-length Ff-gad2 7.96 1.39 times compared exon level H. mycelia, intron contribute Ff-GAD2. Based whole-genome sequencing, analyzed enzyme system related life activities focusing Ff-GAD, synthesis pathway. lay foundation elucidating metabolism developing targeted breeding strategies GABA-producing fungi.

Language: Английский

Laccase: A Green Biocatalyst Offers Immense Potential for Food Industrial and Biotechnological Applications DOI
Tingting Zhai,

Hongwei Wang,

Xiaomin Dong

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(44), P. 24158 - 24169

Published: Oct. 22, 2024

Laccase, a multipurpose biocatalyst, is widely distributed across all kingdoms of life and plays key role in essential biological processes such as lignin synthesis, degradation, pigment formation. These functions are critical for fungal growth, plant-pathogen interactions, maintenance soil health. Due to its broad substrate specificity, multifunctional nature, environmentally friendly characteristics, laccase employed catalyst various green chemistry initiatives. With ability oxidize diverse range phenolic nonphenolic compounds, has also been found be useful food additive assessing quality parameters. Ongoing advancements research technology continually expanding the recognition laccase's potential address global environmental, health, energy challenges. This review aims provide insights into applications laccases biotechnology industry.

Language: Английский

Citations

4
Integrated Multi-Omics Analysis to Investigate the Molecular Mechanisms Underlying the Response of Auricularia heimuer to High-Temperature Stress DOI Creative Commons

Fang Lü,

Xin Sun, Xiaodong Dai

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(3), P. 167 - 167

Published: Feb. 20, 2025

High-temperature stress is a key factor that reduces the yields of edible fungi. Auricularia heimuer (A. heimuer) nutrient-rich fungus widely cultivated in China. In this study, we analyzed physiological, transcriptomic, and metabolomic results A. (variety “Hei29”) under high-temperature stress. Our findings revealed high temperatures (30 °C 35 °C) significantly reduced hyphal growth, increased malondialdehyde content antioxidant enzyme activity, enhanced accumulation secondary metabolites, such as phenolic compounds flavonoids. A total 15 candidate genes potentially responsive to were identified through transcriptomic analysis, including those involved regulating defense, heat shock response, sugar metabolism, amino acid accumulating metabolites. Metabolomic analysis three metabolites stress, kinetin, flavonoids, caffeic acid, well several metabolic pathways, nucleotide ABC transporters, cofactor biosynthesis. These mechanisms help mitigate oxidative damage cellular structures energy deficits caused by elevated temperatures, enabling maintain stability, function, growth This study first explore molecular mechanism response The provide valuable insights into tolerance heimuer, highlighting potential targets for developing heat-tolerant strains industrial application.

Language: Английский

Citations

0
Gene Editing in Ganoderma lucidum: Development, Challenges, and Future Prospects DOI Creative Commons

Sirong He,

Yuanchao Liu, Zhang Zhi

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 310 - 310

Published: April 14, 2025

As an emerging and innovative technology, gene-editing technology has been widely applied in crop breeding, human disease treatment, animal model research, drug vaccine development, microbial engineering. We mainly introduce the development of application clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) Ganoderma lucidum current challenges optimization strategies use as well status breeding. Finally, future research directions that gene editing may explore breeding are prospects given existing background, directions, prospects.

Language: Английский

Citations

0
Microbial Community Response and Assembly Process of Yellow Sand Matrix in a Desert Marginal Zone Under Morchella Cultivation DOI Creative Commons

Cuicui Su,

Shengyin Zhang,

Yanfang Zhou

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 921 - 921

Published: April 16, 2025

In this study, we investigated the adaptation of yellow-sand-substrate Morchella cultivation in desert fringe and its effect on soil physicochemical properties microbial communities. The qPCR high-throughput sequencing with null modeling analyzed diversity, networks, assembly under nutrient supplementation, linking changes to dynamics. results showed that yellow sand substrate can be planted area, as bags resulted a yield 691 g/m2 fruit units. Cultivation could significantly increase substrate, such organic matter (SOM), total nitrogen (TN), ammonium (NH4+−N), amount carbon (MBC/MBN). fungal community was dominated by Ascomycota, Basidiomycota, Firmicutes, Bacteroidota, Actinobacteriota. RDA analysis Ascomycota Proteobacteria were positively correlated NH4+−N, MBN, SOM, MBC, acting potassium (AK), TN, C/N. promoted positive correlation-dominant network pattern substrate. bag treatment reduced bacterial complexity while enhancing complexity, connectivity stability, accompanied significant increases Proteobacteria, Cladosporium, Thermomyces relative abundances during until original degradation. Deterministic processes communities, morel drove toward heterogeneous selection processes. study revealed economic value application potential improving sandy soil, which is great importance for practical mushrooms desert.

Language: Английский

Citations

0
Plant Secondary Metabolites—Central Regulators Against Abiotic and Biotic Stresses DOI Creative Commons
Ameer Khan,

Farah Kanwal,

Sana Ullah

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(4), P. 276 - 276

Published: April 16, 2025

As global climates shift, plants are increasingly exposed to biotic and abiotic stresses that adversely affect their growth development, ultimately reducing agricultural productivity. To counter these stresses, produce secondary metabolites (SMs), which critical biochemical essential compounds serve as primary defense mechanisms. These diverse compounds, such alkaloids, flavonoids, phenolic nitrogen/sulfur-containing act natural protectants against herbivores, pathogens, oxidative stress. Despite the well-documented protective roles of SMs, precise mechanisms by environmental factors modulate accumulation under different stress conditions not fully understood. This review provides comprehensive insights into recent advances in understanding functions SMs plant emphasizing regulatory networks biosynthetic pathways. Furthermore, we explored unique contributions individual SM classes responses while integrating findings across entire spectrum diversity, providing a resilience multiple conditions. Finally, highlight emerging strategies for harnessing improve crop through genetic engineering present novel solutions enhance sustainability changing climate.

Language: Английский

Citations

0
Genome editing tools based improved applications in macrofungi DOI
Deepali Jain, Anu Kalia, Shivani Sharma

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: July 30, 2024

Language: Английский

Citations

2
Biological Characteristics of the Mycelium and Optimization of the Culture Medium for Phallus dongsun DOI Creative Commons
Chao Kang, Xuan Zheng,

W M Wang

et al.

Polish Journal of Microbiology, Journal Year: 2024, Volume and Issue: 73(2), P. 237 - 252

Published: June 1, 2024

Abstract This study aimed to elucidate the influence of various culture medium components, including carbon sources, nitrogen inorganic salts, suspension agents, and temperature, on mycelial growth characteristics Phallus dongsun . Employing single-factor experiments response surface methodology within glass Petri dishes, research identified that carrot powder, soybean ZnSO 4 notably enhanced proliferation aerial mycelium, significantly augmenting rate P. mycelium. The resultant mycelium was observed be dense, robust, fluffy in texture. In particular, markedly accelerated rate. Furthermore, xanthan gum found effectively modulate medium’s viscosity, ensuring a stable facilitating nutrient equilibrium. optimal cultivation temperature determined 25°C, with ceasing below 5°C perishing at temperatures exceeding 35°C. composition established as follows: wheat starch 5 g/l, powder 7.50 glucose 10 0.71 NH Cl 0.68 0.5 agar 20 g/l. Under these optimized conditions, exhibited rapid (1.04 ± 0.14 mm/day), characterized by thick, well-developed structure. investigation provides theoretical foundation for conservation, strain selection, breeding

Language: Английский

Citations

1
Diversity of Host Species and Optimized Cultivation Practices for Enhanced Bioactive Compound Production in Cordyceps militaris DOI Creative Commons
Trung Quang Nguyen,

Phan Duong Thuc Quyen,

Nguyễn Thị Ngọc

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(18), P. 8418 - 8418

Published: Sept. 19, 2024

Cordyceps militaris, recognized for its diverse and potent medicinal properties, plays a critical role in herbal medicine. This study investigates the genus Cordyceps, particularly focusing on C. species widely utilized East Asian traditional medicine therapeutic properties. militaris is rich bioactive compounds, including cordycepin, adenosine, polysaccharides, ergosterol, mannitol, which contribute to broad physiological activities. The research aims explore diversity of host cultivation assess their impact biological activity chemical composition fungus. highlights variability efficacy compounds due different conditions species, emphasizing need standardized practices. Advances genetic engineering fermentation technology have significantly enhanced production these metabolites, supporting sustainable scale-up cultivation. Future should continue molecular mechanisms identify new clinical applications, improve efficiency environmental sustainability. comprehensive review provides insights researchers, healthcare professionals, cultivators optimizing commercial applications.

Language: Английский

Citations

1
Whole-Genome Sequence Analysis of Flammulina filiformis and Functional Validation of Gad, a Key Gene for γ-Aminobutyric Acid Synthesis DOI Creative Commons
Wenyun Li, Junjun Shang, Dapeng Bao

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(12), P. 862 - 862

Published: Dec. 12, 2024

Flammulina filiformis is one of the widely produced edible fungi worldwide. It rich in γ-aminobutyric acid (GABA), a non-protein amino with important physiological functions humans. To investigate key genes GABA metabolic pathway F. filiformis, we isolated monokaryon Fv-HL23-1 from factory-cultivated strain Fv-HL23 and then sequenced assembled genome using PacBio Sequel Illumina NovaSeq sequencing platforms. The results showed that comprised 140 scaffolds total length 40.96 Mb, GC content 49.62%, an N50 917,125 bp, 14,256 protein-coding genes. Phylogenetic analysis based on whole revealed close evolutionary relationship Armillaria mellea, Lentinula edodes, Schizophyllum commune. A 589 carbohydrate-active enzymes were identified Fv-HL23-1, suggesting its strong lignocellulose degradation ability, 108 CYP450 gene family members identified, such as resistance to stress, secondary metabolite synthesis, growth development. proteins glutamate decarboxylase 1 (Ff-GAD1) 2 (Ff-GAD2), which may be responsible for by protein alignment. Molecular docking Ff-GAD2 have better catalytic activity than Ff-GAD1. verify function Ff-gad2, heterologous expression mycelia mononuclear Hypsizigus marmoreus was analyzed. Compared wild type, increased 85.40–283.90%, rate 9.39 ± 2.35%, fresh weight 18.44 7.57%. play role synthesis. In addition, full-length Ff-gad2 7.96 1.39 times compared exon level H. mycelia, intron contribute Ff-GAD2. Based whole-genome sequencing, analyzed enzyme system related life activities focusing Ff-GAD, synthesis pathway. lay foundation elucidating metabolism developing targeted breeding strategies GABA-producing fungi.

Language: Английский

Citations

1