Journal of Dairy Research,
Год журнала:
2025,
Номер
unknown, С. 1 - 9
Опубликована: Март 13, 2025
Abstract
This
research
paper
presents
the
characterization
of
an
enterocin-producing
Enterococcus
durans
MF5
isolate
and
determination
in
vitro
antilisterial
activity
enterocin
produced
by
this
isolate,
named
Ent-MF5.
PCR-based
screening
for
bacteriocin
biosynthetic
genes
revealed
that
E.
harbors
multiple
enterocin-encoding
(
ent
A,
B,
P
X),
classified
as
class
II
bacteriocins
enterocin-P
faecium
(sharing
up
to
99%
similarity
at
genetic
level).
is
sensitive
eight
clinically
important
antibiotics
does
not
possess
cytolysin
activator
-
cyl
gelatinase
gel
E
hyaluronidase
hy
lA
virulence
genes.
The
Ent-MF5
was
abolished
trypsin,
α-chymotrypsin,
protease
proteinase-K.
showed
thermal
pH
stability.
In
addition,
unaffected
presence
various
surfactants
(1%
SDS,
Triton
X-100,
Tween
20,
80).
exhibited
antimicrobial
against
Listeria
monocytogenes
,
innocua
ivanovii
seeligeri
concentrations
low
0.13
μg/ml.
had
a
bactericidal
effect
L.
with
significant
reduction
surviving
cells
equal
or
greater
than
A
75–100%
growth
determined
CFU
counts
observed
following
treatment
4.47
μg/ml
time
points
starting
2
4
h,
respectively.
action
associated
cell
membrane
damage,
flow
cytometry
fluorescence
microscopy.
Thus,
effective
stability
promising
perspectives
application
biopreservatives
food
industry.
Foods,
Год журнала:
2023,
Номер
12(18), С. 3339 - 3339
Опубликована: Сен. 6, 2023
Listeria
monocytogenes
(LM)
is
a
bacterial
pathogen
responsible
for
listeriosis,
foodborne
illness
associated
with
high
rates
of
mortality
(20–30%)
and
hospitalisation.
It
particularly
dangerous
among
vulnerable
groups,
such
as
newborns,
pregnant
women
the
elderly.
The
persistence
this
organism
in
food-associated
environments
months
to
years
has
been
linked
several
devastating
listeriosis
outbreaks.
may
also
result
significant
costs
food
businesses
economies.
Currently,
mechanisms
that
facilitate
LM
are
poorly
understood.
Unravelling
enigma
what
drives
listerial
will
be
critical
developing
more
targeted
control
prevention
strategies.
One
prevailing
hypothesis
persistent
strains
exhibit
stronger
biofilm
production
on
abiotic
surfaces
environments.
This
review
aims
(i)
provide
comprehensive
overview
research
relationship
between
formation
from
phenotypic
whole-genome
sequencing
(WGS)
studies;
(ii)
highlight
ongoing
challenges
determining
role
development
plays
persistence,
if
any;
(iii)
propose
future
directions
overcoming
these
challenges.
Journal of Food Quality,
Год журнала:
2021,
Номер
2021, С. 1 - 10
Опубликована: Ноя. 23, 2021
Food
safety
refers
to
preparing,
transporting,
and
storing
food
avoid
foodborne
sickness
harm.
From
farm
factory
fork,
items
may
meet
various
health
dangers.
Therefore,
is
crucial
both
monetarily
morally.
The
implications
of
failing
comply
with
requirements
are
varied.
requirement
for
accurate,
quick,
nonpartisan
quality
assessments
these
features
in
products
continues
rise
increased
demands
dietary
materials
high-quality
requirements.
Computer
vision
provides
an
automatic,
nondestructive,
economic
approach
achieving
aims.
A
substantial
research
has
demonstrated
its
effectiveness
fruit
vegetable
assessment
classification.
It
stresses
the
critical
components
image
processing
technology
a
survey
most
current
advances
across
sector.
This
article
outlines
essential
parts
computer
system.
In
order
disease
ensure
security,
fast
effective
detection
pathogenic
microorganisms
public
biomonitoring.
Over
years,
microorganism
techniques
have
evolved.
FEMS Microbiology Reviews,
Год журнала:
2023,
Номер
47(4)
Опубликована: Июль 1, 2023
Free
fatty
acids
(FFAs)
have
long
been
acknowledged
for
their
antimicrobial
activity.
More
recently,
long-chain
FFAs
(>12
carbon
atoms)
are
receiving
increased
attention
potent
antivirulence
activity
against
pathogenic
bacteria.
In
the
gastrointestinal
tract,
foodborne
pathogens
encounter
a
variety
of
derived
from
diet,
metabolic
activities
gut
microbiota,
or
host.
This
review
highlights
role
as
signaling
molecules
acting
to
inhibit
infectious
potential
important
pathogens,
including
Salmonella
and
Listeria
monocytogenes.
Various
interact
with
sensory
proteins
transcriptional
regulators
controlling
expression
infection-relevant
genes.
Consequently,
may
act
disarm
bacterial
virulence
factors.
Understanding
how
sense
respond
enable
design
new
anti-infective
approaches.
Pathogens,
Год журнала:
2023,
Номер
12(3), С. 444 - 444
Опубликована: Март 12, 2023
Listeria
monocytogenes
is
a
foodborne
pathogen
exhibiting
high
mortality
rate.
In
addition
to
the
robust
tolerance
environmental
stress,
ability
of
L.
develop
biofilms
increases
risk
contaminating
food
processing
facilities
and
ultimately
foods.
This
study
aims
synergistic
approach
better
control
using
nisin,
only
bacteriocin
approved
as
preservative,
in
combination
with
gallic-acid-rich
plant
extracts.
Biofilm
assays
presence
nisin
gallic
acid
or
its
derivatives
revealed
that
significantly
decreased
level
biofilm
formation
monocytogenes,
whereas
ethyl
gallate,
propyl
lauryl
gallate
enhanced
production.
As
widely
distributed
plants,
we
examined
whether
extracts
from
such
clove,
chestnut,
oregano,
sage,
may
generate
similar
antibiofilm
effects.
Remarkably,
sage
activity
against
monocytogenes;
however,
other
tested
increased
formation,
particularly
at
concentrations.
Moreover,
combinations
reduced
on
stainless
steel.
Sage
common
spice
has
various
beneficial
health
effects,
including
antioxidation
anti-cancer
properties.
The
findings
this
demonstrate
can
be
potentially
combined
prevent
production
monocytogenes.
PesquisAgro,
Год журнала:
2025,
Номер
8(1), С. 125 - 136
Опубликована: Янв. 7, 2025
Os
biofilmes
são
comunidades
de
células
microbianas
envoltas
por
uma
matriz
exopolissacarídeos
(EPS),
que
é
responsável
pela
adesão
dos
microrganismos
a
superfícies.
Um
microrganismo
frequentemente
produz
essa
estrutura
e
persiste
em
plantas
processamento
na
indústria
alimentícia
Listeria
innocua.
Este
estudo
avaliou
capacidade
innocua
formar
biofilme
superfícies
polipropileno
sob
diferentes
temperaturas
(7°C,
27°C,
37°C
42°C)
ao
longo
cinco
dias.
Para
quantificação
biofilmes,
foram
utilizados
os
meios
Ágar
Ottaviani
&
Agosti
(ALOA)
Oxford
(MOX),
ambos
empregados
detecção
spp.
resultados
mostraram
houve
desenvolvimento
todas
as
testadas,
com
contagens
significativamente
mais
altas
observadas
27°C
37°C.
Isso
sugere
essas
faixas
temperatura
ideais
para
formação
Essas
condições
térmicas
coincidem
aquelas
encontradas
áreas
alimentos,
o
aumenta
risco
contaminação
cruzada
persistência
contato
alimentos.
Biofilm,
Год журнала:
2022,
Номер
4, С. 100075 - 100075
Опубликована: Апрель 19, 2022
The
increase
in
human
consumption
of
animal
proteins
implies
changes
the
management
meat
production.
This
is
followed
by
increasingly
restrictive
regulations
on
antimicrobial
products
such
as
chemical
biocides
and
antibiotics,
used
particular
to
control
pathogens
that
can
spread
zoonotic
diseases.
Aligned
with
One
Health
concept,
alternative
biological
solutions
are
under
development
starting
be
Beneficial
bacteria
able
form
positive
biofilms
guide
surface
microbial
ecology
limit
pathogen
settlement
promising
tools
could
complement
existing
biosecurity
practices
maintain
hygiene
livestock
buildings.
Although
benefits
have
already
been
documented,
associated
fundamental
mechanisms
rationale
composition
these
new
still
sparce.
review
provides
an
overview
envisioned
modes
action
building
surfaces
resulting
criteria
for
selection
appropriate
microorganisms
this
specific
application.
Limits
advantages
approach
discussed
well
impact
along
food
chain,
from
farm
fork.
Food Control,
Год журнала:
2022,
Номер
137, С. 108971 - 108971
Опубликована: Март 17, 2022
This
study
evaluated
the
capacity
of
real-time
PCR
and
conventional
culture
methods
to
detect
Salmonella
enterica
serovar
Typhimurium
Listeria
monocytogenes
adhered
stainless
steel
surfaces
used
as
food
contact
surfaces.
The
adhesion
microorganisms
was
performed
under
dry
conditions
represent
stress
which
these
pathogens
can
be
subjected
in
processing
environment.
samples
were
analyzed
with
various
pre-enrichment
times:
S.
Typhymurium
(0,
6,
18
h)
L.
25
procedures
concentrating
or
not
after
pre-enrichments.
results
showed
that
obtained
increased
than
method
a
low
number
both
pathogens,
even
detected
without
pre-enrichment.
However,
is
recommended
avoid
detection
false
positives
from
dead
cells
during
ensure
absence
negatives
due
initial
concentrations.
concentration
adhering
bacteria
frequency
positive
for
Typhimurium,
but
this
effect
observed
case
monocytogenes.
