DPPH Radical Scavenging Assay

Processes, Год журнала: 2023, Номер 11(8), С. 2248 - 2248

Опубликована: Июль 26, 2023

Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals human metabolism and their deterioration during processing storage fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, has been a parallel increase use assays estimate antioxidant efficacy food systems. many bioanalytical methods that measure effect. Of these, 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay most putative, popular, commonly used method determine ability. this review, general approach DPPH radical scavenging taken. context, studies, including attempts adapt different analytes, search for highest activity values, optimize measurement, have previously performed. Therefore, it highly important introduce measures aimed at standardizing conditions activity, various reaction media suitable assay. For aim, chemical basic principles defined discussed outline. addition, study describes defines sections biological Additionally, some chemical, critical, technical details removal given. This simple which prospective compounds or herbal extracts mixed with solution absorbance measured after certain period. However, despite rapid advances instrumental techniques analysis, not undergone extreme modification. presents detailed information about in-depth review developments.

Язык: Английский

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Synthesis and inhibition profiles of N-benzyl- and N-allyl aniline derivatives against carbonic anhydrase and acetylcholinesterase – A molecular docking study

Arabian Journal of Chemistry, Год журнала: 2021, Номер 15(3), С. 103645 - 103645

Опубликована: Дек. 22, 2021

The alkyl and aryl derivatives of aniline are important starting materials in fine organic synthesis. Allyl bromide benzyl chloride were taken as substrates for the alkylation reaction a halide ion scavenger. Triethylamine was utilized at reflux condition N,N-dimethylacetamide (DMA). Novel synthesized N-benzyl N-allyl (1a-f) evaluated to be highly potent inhibitors acetylcholinesterase (AChE) carbonic anhydrases (hCAs). half maximal inhibitory concentration (IC50) N-benzyl- calculated between 243.11 633.54 nM hCA I, 296.32–518.37 II 182.45–520.21 AChE enzymes. On other hand, Ki values range 149.24 ± 15.59 519.59 102.27 AChE, 202.12 16.21 635.31 45.33 I 298.57 94.13 511.18 115.98 isoenzyme. Additionally, silico molecular docking computations performed with Autodock Vina program support experimental vitro studies both hCAs inhibitors. results demonstrated that scores good agreement results.

Язык: Английский

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Comprehensive Metabolite Profiling of Berdav Propolis Using LC-MS/MS: Determination of Antioxidant, Anticholinergic, Antiglaucoma, and Antidiabetic Effects

Molecules, Год журнала: 2023, Номер 28(4), С. 1739 - 1739

Опубликована: Фев. 11, 2023

Propolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It rich in phenolic flavonoid compounds, which contain antioxidant, antimicrobial, anticancer properties. In this study, the chemical composition antioxidant activities of propolis were investigated; ABTS

Язык: Английский

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Screening of Antiglaucoma, Antidiabetic, Anti-Alzheimer, and Antioxidant Activities of Astragalus alopecurus Pall—Analysis of Phenolics Profiles by LC-MS/MS

Pharmaceuticals, Год журнала: 2023, Номер 16(5), С. 659 - 659

Опубликована: Апрель 28, 2023

Astragalus species are traditionally used for diabetes, ulcers, leukemia, wounds, stomachaches, sore throats, abdominal pain, and toothaches. Although the preventive effects of against diseases known, there is no record therapeutic alopecurus. In this study, we aimed to evaluate in vitro antiglaucoma, antidiabetic, anti-Alzheimer's disease, antioxidant activities methanolic (MEAA) water (WEAA) extracts aerial part A. Additionally, its phenolic compound profiles were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). MEAA WEAA evaluated their inhibition ability on α-glycosidase, α-amylase, acetylcholinesterase (AChE), human carbonic anhydrase II (hCA II) enzymes. The compounds LC-MS/MS. Furthermore, total flavonoid contents determined. context, activity was 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), N,N-dimethyl-p-phenylene diamine (DMPD), ferric reducing power (FRAP), cupric ions (Cu2+) capacity (CUPRAC), (Fe3+) reducing, ferrous (Fe2+) chelating methods. had IC50 values 9.07 2.24 μg/mL 693.15 346.58 1.99 2.45 AChE, 147.7 171.7 hCA II. While amounts 16.00 18.50 μg gallic acid equivalent (GAE)/mg extract, both calculated as 66.23 33.115 quercetin (QE)/mg, respectively. showed, respectively, variable DPPH radical scavenging (IC50: 99.02 115.53 μg/mL), ABTS 32.21 30.22 µg/mL), DMPD 231.05 65.22 Fe2+ 46.21 33.01 μg/mL). abilities were, Fe3+ (λ700: 0.308 0.284), FRAP (λ593: 0.284 CUPRAC (λ450: 0.163 0.137). A 35 phenolics scanned, 10 determined LC-MS/MS analysis. revealed that mainly contained isorhamnetin, fumaric acid, rosmarinic derivatives. This first report indicating have abilities, activities. These results demonstrate potential through properties enzyme inhibitor medicine. work provides foundation further research into establishment novel therapeutics glaucoma, Alzheimer's disease.

Язык: Английский

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Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and α-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol

Molecules, Год журнала: 2022, Номер 27(10), С. 3091 - 3091

Опубликована: Май 11, 2022

Coumestrol (3,9-dihydroxy-6-benzofuran [3,2-c] chromenone) as a phytoestrogen and polyphenolic compound is member of the Coumestans family quite common in plants. In this study, antiglaucoma, antidiabetic, anticholinergic, antioxidant effects were evaluated compared with standards. To determine activity coumestrol, several methods—namely N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD•+)-scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS•+)-scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH•)-scavenging potassium ferric cyanide reduction ability, cupric ion (Cu2+)-reducing activity—were performed. Butylated hydroxyanisole (BHA), Trolox, α-Tocopherol, butylated hydroxytoluene (BHT) used reference antioxidants for comparison. scavenged DPPH an IC50 value 25.95 μg/mL (r2: 0.9005) while BHA, BHT, α-Tocopherol demonstrated values 10.10, 25.95, 7.059, 11.31 μg/mL, respectively. When these results evaluated, had similar DPPH•-scavenging effect to BHT lower better than BHA α-tocopherol. addition, inhibition tested against metabolic enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II), α-glycosidase, which are associated some global diseases such Alzheimer’s disease (AD), glaucoma, diabetes. exhibited Ki 10.25 ± 1.94, 5.99 1.79, 25.41 1.10, 30.56 3.36 nM towards enzymes,

Язык: Английский

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Evaluation of the Antioxidant and Antiradical Properties of Some Phyto and Mammalian Lignans

Molecules, Год журнала: 2021, Номер 26(23), С. 7099 - 7099

Опубликована: Ноя. 24, 2021

In this study, the antioxidant and antiradical properties of some phyto lignans (nordihydroguaiaretic acid, secoisolariciresinol, secoisolariciresinol diglycoside, α-(-)-conidendrin) mammalian (enterodiol enterolactone) were examined by different assays. For purpose, radical scavenging activities realized 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS•+) assay 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Additionally, reducing ability evaluated cupric ions (Cu2+) (CUPRAC) ability, ferric (Fe3+) [Fe3+-(TPTZ)2]3+ complex (FRAP) abilities. Also, half maximal inhibitory concentration (IC50) values determined reported for DPPH• ABTS•+ influences all lignan molecules. The absorbances found in range 0.150-2.320 Fe3+ reducing, 0.040-2.090 Cu2+ 0.360-1.810 FRAP On other hand, IC50 ranges 6.601-932.167 µg/mL 13.007-27.829 scavenging. used bioanalytical methods, lignans, as secondary metabolites plants, demonstrated considerably higher activity compared to that lignans. addition, it was observed enterodiol enterolactone exhibited relatively weaker when or standard antioxidants, including butylated hydroxytoluene (BHT), hydroxyanisole (BHA), Trolox, α-tocopherol.

Язык: Английский

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Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.)

Biocatalysis and Agricultural Biotechnology, Год журнала: 2022, Номер 43, С. 102417 - 102417

Опубликована: Июль 6, 2022

Язык: Английский

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Antioxidant, Antidiabetic, Anticholinergic, and Antiglaucoma Effects of Magnofluorine

Molecules, Год журнала: 2022, Номер 27(18), С. 5902 - 5902

Опубликована: Сен. 11, 2022

Magnofluorine, a secondary metabolite commonly found in various plants, has pharmacological potential; however, its antioxidant and enzyme inhibition effects have not been investigated. We investigated the potential of Magnofluorine using bioanalytical assays with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS

Язык: Английский

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Isolation of Some Phenolic Compounds from Plantago subulata L. and Determination of Their Antidiabetic, Anticholinesterase, Antiepileptic and Antioxidant Activity

Chemistry & Biodiversity, Год журнала: 2022, Номер 19(8)

Опубликована: Июль 7, 2022

Abstract In the current study, some phenolic compounds, including acteoside, isoacteoside, echinacoside, and arenarioside purified characterized from Plantago subulata . These compounds were tested for its antioxidant potential, Fe 3+ Cu 2+ reductive ability chelating effects. The inhibitory effects of isolated towards human carbonic anhydrase I II isoenzymes (hCA hCA II), butyrylcholinesterase (BChE) acetylcholinesterase (AChE), aldose reductase (AR) α‐glycosidase (α‐gly). K i values found these in range 0.24±0.05–1.38±0.34 μM against I, 0.194±0.018–1.03±0.06 II, 0.043±0.01–0.154±0.02 AChE, 3.92±1.08–11.93±4.45 BChE, 0.082±0.0008–1.68±0.42 AR, 6.93±2.74–17.17±6.70 α‐glycosidase. As a result, displayed inhibition studied all metabolic enzymes. They are promising candidates treating disorders like Alzheimer's disease, diabetes mellitus, glaucoma, leukemia, epilepsy.

Язык: Английский

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Polyphenol Contents, Potential Antioxidant, Anticholinergic and Antidiabetic Properties of Mountain Mint (Cyclotrichium leucotrichum)

Chemistry & Biodiversity, Год журнала: 2022, Номер 19(3)

Опубликована: Янв. 11, 2022

In the present work, antioxidant and antidiabetic potentials of mountain mint [Cyclotrichium leu-cotrichum (Stapf ex Rech. Fil.) Leblebici] was first time appraised. this sense, methanol (MECL) water (WECL) extracts were obtained from aerial parts (Cyclotrichium leucotrichum) studied for their ability by several bioanalytical assays. Also, inhibition profiles realized toward metabolic enzymes connected to some diseases, including butyrylcholinesterase (BChE), α-glycosidase, acetylcholinesterase (AChE), α-amylase enzymes. Additionally, phenolic contents determined putative chromatographic method LC/MS/MS. Consequently, nineteen molecules identified in MECL fifteen found WECL. effects both using methods 1,1-diphenyl-2-picryl-hydrazyl (DPPH⋅), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+ ) N,N-dimethyl-p-phenylenediamine (DMPD.+ scavenging activities, ferric (Fe3+ cupric (Cu2+ ions Fe3+ -2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) reducing capacities. WECL as powerful DPPH⋅ (IC50 : 23.74 28.85 μg/mL), ABTS.+ 12.53 14.05 μg/mL) DMPD.+ 43.52 54.80 μg/mL). demonstrated effective on AChE 69.31 115.51 BChE 57.75 86.62 α-glycosidase 36.47 62.94 1.01 3.43 This study will be useful future studies determine properties enzyme profile food, medical industrially important plants.

Язык: Английский

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