Experimental Eye Research, Год журнала: 2021, Номер 214, С. 108892 - 108892
Опубликована: Дек. 10, 2021
Язык: Английский
Journal of Controlled Release, Год журнала: 2022, Номер 347, С. 533 - 543
Опубликована: Май 24, 2022
Язык: Английский
Процитировано
355
Methods and Protocols, Год журнала: 2021, Номер 4(1), С. 10 - 10
Опубликована: Янв. 20, 2021
MicroRNAs (miRNAs) represent a family of short non-coding regulatory RNA molecules that are produced in tissue and time-specific manner to orchestrate gene expression post-transcription. MiRNAs hybridize target mRNA(s) induce translation repression or mRNA degradation. Functional studies have demonstrated miRNAs engaged virtually every physiological process and, consequently, miRNA dysregulations been linked multiple human pathologies. Thus, mimics anti-miRNAs restore downregulate aberrantly expressed miRNAs, respectively, highly sought-after therapeutic strategies for effective manipulation levels. In this regard, carrier vehicles facilitate proficient safe delivery miRNA-based therapeutics fundamental the clinical success these pharmaceuticals. Here, we highlight strengths weaknesses current state-of-the-art viral non-viral systems provide perspective on how tools can be exploited improve outcomes therapeutics.
Язык: Английский
Процитировано
220
Journal of Nanobiotechnology, Год журнала: 2022, Номер 20(1)
Опубликована: Июнь 14, 2022
Abstract Cancer is a leading public health problem worldwide. Its treatment remains daunting challenge, although significant progress has been made in existing treatments recent years. A large concern the poor therapeutic effect due to lack of specificity and low bioavailability. Gene therapy recently emerged as powerful tool for cancer therapy. However, delivery methods limit its effects. Exosomes, subset extracellular vesicles secreted by most cells, have characteristics good biocompatibility, toxicity immunogenicity, great designability. In past decades, carriers diagnostic markers, they caught extensive attention. This review introduced exosomes, focused on their applications DNA, messenger RNA (mRNA), microRNA (miRNA), small interfering (siRNA), circular (circRNA) other nucleic acids. Meanwhile, application exosome-based clinical trials were presented discussed. Through systematic summarization analysis, advances current challenges exosome-mediated acid are introduced, which will provide theoretical basis development drugs. Graphical
Язык: Английский
Процитировано
189
Journal of Controlled Release, Год журнала: 2022, Номер 353, С. 1127 - 1149
Опубликована: Дек. 26, 2022
Язык: Английский
Процитировано
162
Nanomaterials, Год журнала: 2021, Номер 11(6), С. 1481 - 1481
Опубликована: Июнь 3, 2021
Exosomes as nanosized vesicles are emerging drug delivery systems for therapeutics owing to their natural origin, ability mediate intercellular communication, and potential encapsulate various biological molecules such proteins nucleic acids within the lipid bilayer membrane or in lumen. contain endogenous components (proteins, lipids, RNA) that could be used deliver cargoes target cells, offering an opportunity diagnose treat diseases. Owing travel safely extracellular fluid transport cells with high efficacy, exosomes offer enhanced of vivo. However, several challenges related stabilization exosomes, production sufficient amounts safety efficient loading drugs into clearance from circulation, transition bench scale clinical may limit development use. For use it is important understand molecular mechanisms behind function exosome vesicles. This review exploits techniques isolation characterization enhance therapeutic outcome methods. Further, routes administration, trials, regulatory aspects will discussed this review.
Язык: Английский
Процитировано
142
ACS Biomaterials Science & Engineering, Год журнала: 2023, Номер 9(2), С. 577 - 594
Опубликована: Янв. 9, 2023
Exosomes are the phospholipid-membrane-bound subpopulation of extracellular vesicles derived from plasma membrane. The main activity exosomes is cellular communication. In cancer, play an important rolefrom two distinct perspectives, one related to carcinogenesis and other as theragnostic drug delivery tools. outer phospholipid membrane Exosome improves targeting efficiency. . Some vital features such biocompatibility, low toxicity, immunogenicity make it a more exciting system. Exosome-based new innovative approach cancer treatment. Exosome-associated biomarker analysis heralded era diagnostics in specific way. This Review focuses on exosome biogenesis, sources, isolation, interrelationship with exosome-related biomarkers, loading methods, exosome-based biomolecule delivery, advances limitations clinical settings studies. understanding will change diagnostic therapeutic future.
Язык: Английский
Процитировано
130
Cell Communication and Signaling, Год журнала: 2023, Номер 21(1)
Опубликована: Янв. 23, 2023
Abstract MicroRNAs (miRNAs) are a group of small non-coding RNAs that regulate gene expression by targeting mRNA. Moreover, it has been shown miRNAs changed in various diseases, such as cancers, autoimmune disease, infectious and neurodegenerative Diseases. The suppression miRNA function can be easily attained utilizing anti-miRNAs. In contrast, an enhancement achieved through the utilization modified mimetics. discovery appropriate carriers body become interesting subject for investigators. Exosomes (EXOs) therapeutic efficiency safety transferring different cellular biological components to recipient cell have attracted significant attention their capability carriers. Mesenchymal stem cells (MSCs) recognized generate wide range EXOs (MSC-EXOs), showing MSCs may effective EXO generation clinically measure compared other origins. MSC-EXOs widely investigated because immune attributes, tumor-homing flexible characteristics. this article, we summarized features MSC-EXOs, including production, purification, loading methods modification targeted delivery diseases. Graphical abstract
Язык: Английский
Процитировано
75
Experimental & Molecular Medicine, Год журнала: 2024, Номер 56(4), С. 836 - 849
Опубликована: Апрель 1, 2024
Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.
Язык: Английский
Процитировано
72
Cancers, Год журнала: 2023, Номер 15(4), С. 1023 - 1023
Опубликована: Фев. 6, 2023
As the world's most prevalent cancer, breast cancer imposes a significant societal health burden and is among leading causes of death in women worldwide. Despite notable improvements survival countries with early detection programs, combined different modes treatment to eradicate invasive disease, current chemotherapy regimen faces challenges associated chemotherapy-induced side effects development drug resistance. Therefore, serious concerns regarding chemotherapeutics are pressuring researchers develop alternative therapeutics better efficacy safety. Due their extremely biocompatible nature efficient destruction cells via numerous mechanisms, phytochemicals have emerged as one attractive therapies for treat cancer. Additionally, phytofabricated nanocarriers, whether used alone or conjunction other loaded phytotherapeutics chemotherapeutics, showed promising results treating In review, we emphasize anticancer activity phytochemical-instigated nanocarriers phytochemical-loaded against both vitro vivo. Since diverse mechanisms implicated phytochemicals, strong emphasis placed on pathways underlying action. Furthermore, discuss selective targeted delivery consider research gaps, recent developments, druggability phytoceuticals. Combining phytochemical chemotherapeutic agents nanotechnology might far-reaching impacts future.
Язык: Английский
Процитировано
55
International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(17), С. 9543 - 9543
Опубликована: Сен. 2, 2021
Exosomes are becoming increasingly important therapeutic biomaterials for use in a variety of applications due to their unique characteristics, especially the ineffectiveness and cytotoxicity some existing therapies synthetic nanocarriers. They highly promising as carriers drugs, genes, other agents that can be incorporated into interior or onto surface through various modification techniques improve targeting abilities. In addition, they biocompatible, safe, stable. The review focuses on different types exosomes methods preparation, including incorporation kinds cargo, drug delivery purposes. particular, importance effectiveness vehicles applications, diseases disorders such cancer treatment, cardiovascular neurodegenerative diseases, emphasized. Administration routes body also included. A novelty article is emphasis global companies already successfully developing testing biomaterials, with focus most influential ones. Moreover, comparison advantages disadvantages exosome production summarized first time.
Язык: Английский
Процитировано
100