Role of IL-27 in COVID-19: A Thin Line between Protection and Disease Promotion DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Angela Santoni

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7953 - 7953

Опубликована: Июль 21, 2024

Cytokine storm is usually described as one of the main reasons behind COVID-associated mortality. Cytokines are essential protein molecules engaged in immune responses; they play a critical role protection against infections. However, also contribute to inflammatory reactions and tissue damage, becoming double-edged sword context COVID-19. Recent studies have suggested various cytokines chemokines that crucial response SARS-CoV-2 infection. One such cytokine interleukin 27 (IL-27), which has been found be elevated blood plasma patients with Within this study, we will explore IL-27 responses analyze both existing literature our own prior research findings on It affects wide variety cells. Regardless pathological process it involved in, for upholding necessary balance between damage cytotoxicity infectious agents and/or tumors. In COVID-19, multiple processes, including antiviral via CD8+ cells, IgG subclass switching, even activation Tregs.

Язык: Английский

Tuberculosis and COVID-19 Dually Affect Human Th17 Cell Immune Response DOI Creative Commons
Анна Старшинова, Igor Kudryavtsev, Artem Rubinstein

и другие.

Biomedicines, Год журнала: 2023, Номер 11(8), С. 2123 - 2123

Опубликована: Июль 27, 2023

COVID-19 infection not only profoundly impacts the detection of tuberculosis (Tbc) but also affects modality in patient immune response. It is important to determine response alterations latent as well SARS-CoV-2-infected patients. Such changes may have underlying effects on development and course further tuberculosis. Here, we aimed review characteristics TB patients or convalescent with (LTBI).We analyzed features For this, publications released from December 2019 March 2023; those which were published accessible international databases ("Medline", "PubMed", "Scopus") keywords such "COVID-19", "SARS-CoV-2", "tuberculosis", "pulmonary tuberculosis", "latent infection", "Treg", "follicular Treg", "Treg subsets", considered.Through our analysis, found that who had been infected previously elevated Th1 Th2 cell levels. High levels cells serve a positive marker, characterizing activated during infection. post-COVID-19 subjects showed decreased Th17 levels, indicating lack development. Moreover, typical associated an increase Treg level, contributes hyperinflammatory response.According data obtained, proceeds dissimilar way due distinct response, elicited by SARS-CoV-2. Importantly, active severe decline Both pathogens lead disturbed responses, increasing risk developing TB. The insights findings this paper be used improve future management individuals

Язык: Английский

Процитировано

7

Macrophage-Derived Chemokine MDC/CCL22: An Ambiguous Finding in COVID-19 DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Арег А. Тотолян

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13083 - 13083

Опубликована: Авг. 23, 2023

Macrophage-derived chemokine (MDC/CCL22) is a of the C-C subfamily. It involved in T-cellular maturation and migration. Our previous research shows that plasma CCL22/MDC tends to show statistically significant depletion concentrations acute patients convalescents when compared healthy donors. In current work, we investigate existing views on MDC/CCL22 dynamics association with various pathologies, including respiratory diseases and, specifically, COVID-19. Additionally, present our explanations for observed decrease The first hypothesis provide implies viral products bind block its activity. Another explanation this phenomenon based dendritic cells population inhibition their function.

Язык: Английский

Процитировано

6

Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still’s Disease DOI Creative Commons
V. Yu. Myachikova, Igor Kudryavtsev, Artem Rubinstein

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(2), С. 1177 - 1191

Опубликована: Фев. 1, 2024

Adult-onset Still’s disease (AOSD) is a complex systemic inflammatory disorder, categorized as an ‘IL-1 driven’ inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted study, enrolling 7 patients with relapsing AOSD 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- B-cell subsets. T-cell subsets were significantly altered in AOSD. Within CD4+ cells, Th2 decreased. Additionally, Th17 cell follicular Th within CD45RA–CD62L+ CD45RA–CD62L– compared controls. identified changes CD8+ maturation ‘polarization’ patients, elevated presence of TEMRA subset. Furthermore, percentage Tc1 was decreased, while frequency CCR6–CXCR3– Tc2 elevated. Finally, we determined that CD5+CD27– dramatically decreased Further investigations on large group are required evaluate these adaptive immunity pathogenesis.

Язык: Английский

Процитировано

2

Role of IL-27 in COVID-19: A Thin Line Between Protection and Disease Promotion DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Angela Santoni

и другие.

Опубликована: Май 30, 2024

: Cytokine storm is usually described as one of the main reasons behind COVID-associated mor-tality. Cytokines are essential protein molecules engaged in immune responses; they play a critical role protection against infections. However, also contribute to inflammatory reactions and tissue damage, becoming double-edged sword context COVID-19. Recent studies have suggested various cytokines chemokines that crucial response SARS-CoV-2 infection. One such cytokine Interleukin 27 (IL-27), which has been found be elevated blood plasma patients with Within this study, we will explore IL-27 responses address our own findings concerning It affects wide variety cells; regardless pathological process it involved in, for upholding necessary balance between damage cytotoxicity in-fectious agents and/or tumors. In COVID-19, multiple processes, including antiviral via CD8+ cells, IgG subclass switching, even activation Tregs.

Язык: Английский

Процитировано

2

Circulating СD8+ T Cells Subsets in Primary Sjögren’s Syndrome DOI Open Access

I. V. Kudryavtsev,

Stanislava Benevolenskaia,

М. К. Серебрякова

и другие.

Опубликована: Авг. 30, 2023

Currently, multiple studies have indicated that CD8+ T lymphocytes play a role in causing damage to the exocrine glands through acinar injury Primary Sjogren’s syndrome (pSS). The aim of this research was assess imbalance subsets within cells peripheral blood. We examined blood samples from 34 patients with pSS and healthy individuals as controls. used flow cytometry enumerate cell maturation stages using markers CD62L, CD28, CD27, CD4, CD8, CD3, CD45RA, CD45. For immunophenotyping ‘polarized’ we following monoclonal antibodies:CXCR5, CCR6,CXCR3 CCR4. findings revealed both relative absolute numbers 'naïve' were higher compared volunteers. Conversely, proportions effector memory notably lower. Furthermore, our data suggested among pSS, levels cytotoxic Tc1 reduced, while frequencies regulatory cytokine-producing Tc2 Tc17 significantly elevated. Simultaneously, displayed negative correlation immunoglobulin G, Rheumatoid factor, Schirmer test, unstimulated saliva flow. On other hand, exhibited positive these parameters. In summary, study immune dysfunction cells, including alterations plays significant development pSS.

Язык: Английский

Процитировано

5

Features of Myocarditis: Morphological Differential Diagnosis in Post-COVID-19 Children DOI Creative Commons
В. Е. Карев,

Anastasia Y. Starshinova,

Anzhela Glushkova

и другие.

Diagnostics, Год журнала: 2023, Номер 13(15), С. 2499 - 2499

Опубликована: Июль 27, 2023

Myocarditis is characterized by dysfunction and destruction of cardiomyocytes, infiltrative inflammation, development fibrosis. Late diagnosis myocarditis has been a serious global health problem, especially due to the spread new coronavirus infection. The aim this review identify differences between viral etiology, including SARS-CoV-2 lesions, based on instrumental pathomorphological findings. Material Methods: We analyzed publications covering period from December 2019 May 2023, published in publicly accessible international databases (“Medline”, “PubMed”, “Scopus”), with queries for keywords “myocarditis”, “children”, “cardiovascular inflammation”, “COVID-19”, “SARS-CoV-2”, “severe acute respiratory syndrome 2”, “differential diagnosis”. Results: It was found that no unambiguous morphological criteria coupled lesions were identified. However, detected histopathological changes such as virus-associated degeneration, apoptosis, cardiomyocyte necrosis, moderate interstitial hyperemia, myocardial tissue oedema, capillary endothelial cell major markers Conclusion: necessary further reconsider diagnose SARS-CoV-2-caused myocarditis, rather than solely relying detecting RNA PCR sole evidence-based criterion. Similar issues accompany diagnostics associated other infections. Evidence an etiological can be provided comprehensive analysis diagnostic obtained, confirming virus exposure, followed distinct clinical symptoms, MRI CT changes, criteria.

Язык: Английский

Процитировано

4

Designing structure and <i>E. coli</i> strain-producer bearing SARS-CoV-2 N, S, M, E protein-related sequence antigen DOI Creative Commons
V. V. Kopat, A. A. Riabchenkova, E. L. Chirak

и другие.

Russian Journal of Infection and Immunity, Год журнала: 2023, Номер 13(4), С. 653 - 662

Опубликована: Авг. 21, 2023

T-cell immune response is extremely important in protecting human body from diverse viral infections. It known that it can ensure clearance and complete recovery patients with humoral immunodeficiency. COVID-19 were found to have primarily directed against SARS-CoV-2 structural S, M, N, E proteins, nucleocapsid protein being most conserved. To assess immunity coronavirus infection evaluate an effectiveness of vaccine candidates, necessary develop optimal diagnostic antigen arising antigenic determinants. A test determine host specific susceptibility should target conserved regions global variants. The study was aimed a structure bearing immunogenic sequences derived proteins obtain Escherichia coli producer strain containing recombinant be subsequently used for assessing antiviral immunity. Developing the performed silico: TepiTool NetMHCIIpan predict identify high affinity epitopes spanning E, S MHC II binding. Several variants constructed, which one selected based on its physicochemical properties: isoelectric point, hydrophobicity index aliphatic index, as well 3D representation built by using I-TASSER. sequence synthesized cloned into pET24a(+) vector. resulting plasmid pCorD_PS transformed E. DH5 followed Rosetta (DE3). strain-producer CorD_PS assessed presence stability IPTG-induced expression elimination antigen-bearing plasmid. Based data, developed consisting proteins. 53 kDa predicted stable aqueous solutions point 9.56 potentially allowing simplify purification cells. Plasmid DNA (6695 bp) encoding final vector obtained. stable, productive obtained move design technique further SARS-CoV-2-specific system.

Язык: Английский

Процитировано

4

Imbalance of SARS-CoV-2-specific CCR6+ and CXCR3+ CD4+ T cells and IFN-γ + CD8+ T cells in patients with Long-COVID DOI
Pedro Martínez‐Fleta, Celeste Marcos, Daniel Jiménez‐Carretero

и другие.

Clinical Immunology, Год журнала: 2024, Номер 264, С. 110267 - 110267

Опубликована: Май 31, 2024

Язык: Английский

Процитировано

1

Alterations in T cell immunity over 6–12 months post-COVID-19 infection in convalescent individuals: a screening study DOI Creative Commons

A. V. Zurochka,

М. А. Добрынина,

Э. А. Сафронова

и другие.

Russian Journal of Infection and Immunity, Год журнала: 2024, Номер 14(4), С. 756 - 768

Опубликована: Окт. 30, 2024

Acute COVID-19 is a viral infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that results in dramatically decreased peripheral blood CD3+T cell count apparently due to alterations of thymic T maturation, can persist long term afterwards. Therefore, we analyzed the levels TRECs (T-cell receptor excision circles), and investigated main subsets convalescents. We performed molecular quantification with “TREC/KREC-AMP PS” kit flow cytometric analysis lymphocytes from three groups patients. The first group contained 109 samples convalescents (6–12 month post-acute COVID-19) normal (TRECn); second was formed (TREClow, n = 29), healthy control (HC, 18). noticed no significant differences between all relative absolute numbers. However, CD4+T frequencies were TREClow TRECn compared HC (40.8% (31.6; 50.1) 46.4% (40.0; 53.0) vs 53.5% (47.36; 56.9), p 0.001 р 0.004, respectively). Furthermore, Th patients (701 cell/1 µL (478; 807) 1005 (700; 1419), 0.020, 876 cell/ 1 (661; 1046), 0.008, Finally, both had increased circulating CD8+T cells — 29.4% (20.7; 39.7) group, 26.5% (21.1; 32.7) 21.3% (17.1; 26.0) controls (p 0.024 0.026, In elevated range (508 (372; 622) 356 (247; 531), 0.044). Thus, showed an imbalance CD4+and level even at 6–12 months SARS-CoV-2 infection, observed changes could be closely related maturation differentiation. Such long-term decline TREC circulation may have profound impact on immune system functions requires immunocorrection therapy.

Язык: Английский

Процитировано

1

Prospective and Longitudinal Analysis of Lymphocyte Subpopulations in SARS-CoV-2 Positive and Negative Pneumonia: Potential Role of Decreased Naïve CD8+ in COVID-19 Patients DOI Creative Commons
Makhabbat Bekbossynova, Lyudmila Akhmaltdinova, Kuanysh Dossybayeva

и другие.

Viruses, Год журнала: 2024, Номер 17(1), С. 41 - 41

Опубликована: Дек. 30, 2024

Background: During the acute phase of COVID-19, a number immunological abnormalities have been reported, but few studies longitudinally analyzed specific subsets peripheral blood lymphocytes. Methods: In this observational, prospective, and longitudinal study, adult patients developing pneumonia during COVID-19 pandemic followed up for 12 months. Peripheral lymphocyte were assessed (with focus on memory markers) at 6 time points after disease onset until Results: A total 76 with (characterized by prevalently interstitial pattern lung inflammation) hospital admission (who completed 12-month follow-up period) recruited in study. They divided into two groups, namely positive (n = 31) negative 45) SARS-CoV-2 PCR test. phase, general immunophenotyping profile was comparable most parameters between these except B cells. When T cells according to expression markers, significant decrease naïve CD8+ observed SARS-CoV-2-positive group phase. Notably, aspect maintained period least 9 Conclusions: seems be associated lower compared virus. This alteration can persist convalescent

Язык: Английский

Процитировано

1