International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7953 - 7953
Опубликована: Июль 21, 2024
Cytokine
storm
is
usually
described
as
one
of
the
main
reasons
behind
COVID-associated
mortality.
Cytokines
are
essential
protein
molecules
engaged
in
immune
responses;
they
play
a
critical
role
protection
against
infections.
However,
also
contribute
to
inflammatory
reactions
and
tissue
damage,
becoming
double-edged
sword
context
COVID-19.
Recent
studies
have
suggested
various
cytokines
chemokines
that
crucial
response
SARS-CoV-2
infection.
One
such
cytokine
interleukin
27
(IL-27),
which
has
been
found
be
elevated
blood
plasma
patients
with
Within
this
study,
we
will
explore
IL-27
responses
analyze
both
existing
literature
our
own
prior
research
findings
on
It
affects
wide
variety
cells.
Regardless
pathological
process
it
involved
in,
for
upholding
necessary
balance
between
damage
cytotoxicity
infectious
agents
and/or
tumors.
In
COVID-19,
multiple
processes,
including
antiviral
via
CD8+
cells,
IgG
subclass
switching,
even
activation
Tregs.
Biomedicines,
Год журнала:
2023,
Номер
11(8), С. 2123 - 2123
Опубликована: Июль 27, 2023
COVID-19
infection
not
only
profoundly
impacts
the
detection
of
tuberculosis
(Tbc)
but
also
affects
modality
in
patient
immune
response.
It
is
important
to
determine
response
alterations
latent
as
well
SARS-CoV-2-infected
patients.
Such
changes
may
have
underlying
effects
on
development
and
course
further
tuberculosis.
Here,
we
aimed
review
characteristics
TB
patients
or
convalescent
with
(LTBI).We
analyzed
features
For
this,
publications
released
from
December
2019
March
2023;
those
which
were
published
accessible
international
databases
("Medline",
"PubMed",
"Scopus")
keywords
such
"COVID-19",
"SARS-CoV-2",
"tuberculosis",
"pulmonary
tuberculosis",
"latent
infection",
"Treg",
"follicular
Treg",
"Treg
subsets",
considered.Through
our
analysis,
found
that
who
had
been
infected
previously
elevated
Th1
Th2
cell
levels.
High
levels
cells
serve
a
positive
marker,
characterizing
activated
during
infection.
post-COVID-19
subjects
showed
decreased
Th17
levels,
indicating
lack
development.
Moreover,
typical
associated
an
increase
Treg
level,
contributes
hyperinflammatory
response.According
data
obtained,
proceeds
dissimilar
way
due
distinct
response,
elicited
by
SARS-CoV-2.
Importantly,
active
severe
decline
Both
pathogens
lead
disturbed
responses,
increasing
risk
developing
TB.
The
insights
findings
this
paper
be
used
improve
future
management
individuals
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(17), С. 13083 - 13083
Опубликована: Авг. 23, 2023
Macrophage-derived
chemokine
(MDC/CCL22)
is
a
of
the
C-C
subfamily.
It
involved
in
T-cellular
maturation
and
migration.
Our
previous
research
shows
that
plasma
CCL22/MDC
tends
to
show
statistically
significant
depletion
concentrations
acute
patients
convalescents
when
compared
healthy
donors.
In
current
work,
we
investigate
existing
views
on
MDC/CCL22
dynamics
association
with
various
pathologies,
including
respiratory
diseases
and,
specifically,
COVID-19.
Additionally,
present
our
explanations
for
observed
decrease
The
first
hypothesis
provide
implies
viral
products
bind
block
its
activity.
Another
explanation
this
phenomenon
based
dendritic
cells
population
inhibition
their
function.
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(2), С. 1177 - 1191
Опубликована: Фев. 1, 2024
Adult-onset
Still’s
disease
(AOSD)
is
a
complex
systemic
inflammatory
disorder,
categorized
as
an
‘IL-1
driven’
inflammasomapathy.
Despite
this,
the
interaction
between
T
and
B
cells
remains
poorly
understood.
We
conducted
study,
enrolling
7
patients
with
relapsing
AOSD
15
healthy
control
subjects,
utilizing
deep
flow
cytometry
analysis
to
examine
peripheral
blood
T-
B-cell
subsets.
T-cell
subsets
were
significantly
altered
in
AOSD.
Within
CD4+
cells,
Th2
decreased.
Additionally,
Th17
cell
follicular
Th
within
CD45RA–CD62L+
CD45RA–CD62L–
compared
controls.
identified
changes
CD8+
maturation
‘polarization’
patients,
elevated
presence
of
TEMRA
subset.
Furthermore,
percentage
Tc1
was
decreased,
while
frequency
CCR6–CXCR3–
Tc2
elevated.
Finally,
we
determined
that
CD5+CD27–
dramatically
decreased
Further
investigations
on
large
group
are
required
evaluate
these
adaptive
immunity
pathogenesis.
:
Cytokine
storm
is
usually
described
as
one
of
the
main
reasons
behind
COVID-associated
mor-tality.
Cytokines
are
essential
protein
molecules
engaged
in
immune
responses;
they
play
a
critical
role
protection
against
infections.
However,
also
contribute
to
inflammatory
reactions
and
tissue
damage,
becoming
double-edged
sword
context
COVID-19.
Recent
studies
have
suggested
various
cytokines
chemokines
that
crucial
response
SARS-CoV-2
infection.
One
such
cytokine
Interleukin
27
(IL-27),
which
has
been
found
be
elevated
blood
plasma
patients
with
Within
this
study,
we
will
explore
IL-27
responses
address
our
own
findings
concerning
It
affects
wide
variety
cells;
regardless
pathological
process
it
involved
in,
for
upholding
necessary
balance
between
damage
cytotoxicity
in-fectious
agents
and/or
tumors.
In
COVID-19,
multiple
processes,
including
antiviral
via
CD8+
cells,
IgG
subclass
switching,
even
activation
Tregs.
Currently,
multiple
studies
have
indicated
that
CD8+
T
lymphocytes
play
a
role
in
causing
damage
to
the
exocrine
glands
through
acinar
injury
Primary
Sjogren’s
syndrome
(pSS).
The
aim
of
this
research
was
assess
imbalance
subsets
within
cells
peripheral
blood.
We
examined
blood
samples
from
34
patients
with
pSS
and
healthy
individuals
as
controls.
used
flow
cytometry
enumerate
cell
maturation
stages
using
markers
CD62L,
CD28,
CD27,
CD4,
CD8,
CD3,
CD45RA,
CD45.
For
immunophenotyping
‘polarized’
we
following
monoclonal
antibodies:CXCR5,
CCR6,CXCR3
CCR4.
findings
revealed
both
relative
absolute
numbers
'naïve'
were
higher
compared
volunteers.
Conversely,
proportions
effector
memory
notably
lower.
Furthermore,
our
data
suggested
among
pSS,
levels
cytotoxic
Tc1
reduced,
while
frequencies
regulatory
cytokine-producing
Tc2
Tc17
significantly
elevated.
Simultaneously,
displayed
negative
correlation
immunoglobulin
G,
Rheumatoid
factor,
Schirmer
test,
unstimulated
saliva
flow.
On
other
hand,
exhibited
positive
these
parameters.
In
summary,
study
immune
dysfunction
cells,
including
alterations
plays
significant
development
pSS.
Diagnostics,
Год журнала:
2023,
Номер
13(15), С. 2499 - 2499
Опубликована: Июль 27, 2023
Myocarditis
is
characterized
by
dysfunction
and
destruction
of
cardiomyocytes,
infiltrative
inflammation,
development
fibrosis.
Late
diagnosis
myocarditis
has
been
a
serious
global
health
problem,
especially
due
to
the
spread
new
coronavirus
infection.
The
aim
this
review
identify
differences
between
viral
etiology,
including
SARS-CoV-2
lesions,
based
on
instrumental
pathomorphological
findings.
Material
Methods:
We
analyzed
publications
covering
period
from
December
2019
May
2023,
published
in
publicly
accessible
international
databases
(“Medline”,
“PubMed”,
“Scopus”),
with
queries
for
keywords
“myocarditis”,
“children”,
“cardiovascular
inflammation”,
“COVID-19”,
“SARS-CoV-2”,
“severe
acute
respiratory
syndrome
2”,
“differential
diagnosis”.
Results:
It
was
found
that
no
unambiguous
morphological
criteria
coupled
lesions
were
identified.
However,
detected
histopathological
changes
such
as
virus-associated
degeneration,
apoptosis,
cardiomyocyte
necrosis,
moderate
interstitial
hyperemia,
myocardial
tissue
oedema,
capillary
endothelial
cell
major
markers
Conclusion:
necessary
further
reconsider
diagnose
SARS-CoV-2-caused
myocarditis,
rather
than
solely
relying
detecting
RNA
PCR
sole
evidence-based
criterion.
Similar
issues
accompany
diagnostics
associated
other
infections.
Evidence
an
etiological
can
be
provided
comprehensive
analysis
diagnostic
obtained,
confirming
virus
exposure,
followed
distinct
clinical
symptoms,
MRI
CT
changes,
criteria.
Russian Journal of Infection and Immunity,
Год журнала:
2023,
Номер
13(4), С. 653 - 662
Опубликована: Авг. 21, 2023
T-cell
immune
response
is
extremely
important
in
protecting
human
body
from
diverse
viral
infections.
It
known
that
it
can
ensure
clearance
and
complete
recovery
patients
with
humoral
immunodeficiency.
COVID-19
were
found
to
have
primarily
directed
against
SARS-CoV-2
structural
S,
M,
N,
E
proteins,
nucleocapsid
protein
being
most
conserved.
To
assess
immunity
coronavirus
infection
evaluate
an
effectiveness
of
vaccine
candidates,
necessary
develop
optimal
diagnostic
antigen
arising
antigenic
determinants.
A
test
determine
host
specific
susceptibility
should
target
conserved
regions
global
variants.
The
study
was
aimed
a
structure
bearing
immunogenic
sequences
derived
proteins
obtain
Escherichia
coli
producer
strain
containing
recombinant
be
subsequently
used
for
assessing
antiviral
immunity.
Developing
the
performed
silico:
TepiTool
NetMHCIIpan
predict
identify
high
affinity
epitopes
spanning
E,
S
MHC
II
binding.
Several
variants
constructed,
which
one
selected
based
on
its
physicochemical
properties:
isoelectric
point,
hydrophobicity
index
aliphatic
index,
as
well
3D
representation
built
by
using
I-TASSER.
sequence
synthesized
cloned
into
pET24a(+)
vector.
resulting
plasmid
pCorD_PS
transformed
E.
DH5
followed
Rosetta
(DE3).
strain-producer
CorD_PS
assessed
presence
stability
IPTG-induced
expression
elimination
antigen-bearing
plasmid.
Based
data,
developed
consisting
proteins.
53
kDa
predicted
stable
aqueous
solutions
point
9.56
potentially
allowing
simplify
purification
cells.
Plasmid
DNA
(6695
bp)
encoding
final
vector
obtained.
stable,
productive
obtained
move
design
technique
further
SARS-CoV-2-specific
system.
Russian Journal of Infection and Immunity,
Год журнала:
2024,
Номер
14(4), С. 756 - 768
Опубликована: Окт. 30, 2024
Acute
COVID-19
is
a
viral
infection
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
that
results
in
dramatically
decreased
peripheral
blood
CD3+T
cell
count
apparently
due
to
alterations
of
thymic
T
maturation,
can
persist
long
term
afterwards.
Therefore,
we
analyzed
the
levels
TRECs
(T-cell
receptor
excision
circles),
and
investigated
main
subsets
convalescents.
We
performed
molecular
quantification
with
“TREC/KREC-AMP
PS”
kit
flow
cytometric
analysis
lymphocytes
from
three
groups
patients.
The
first
group
contained
109
samples
convalescents
(6–12
month
post-acute
COVID-19)
normal
(TRECn);
second
was
formed
(TREClow,
n
=
29),
healthy
control
(HC,
18).
noticed
no
significant
differences
between
all
relative
absolute
numbers.
However,
CD4+T
frequencies
were
TREClow
TRECn
compared
HC
(40.8%
(31.6;
50.1)
46.4%
(40.0;
53.0)
vs
53.5%
(47.36;
56.9),
p
0.001
р
0.004,
respectively).
Furthermore,
Th
patients
(701
cell/1
µL
(478;
807)
1005
(700;
1419),
0.020,
876
cell/
1
(661;
1046),
0.008,
Finally,
both
had
increased
circulating
CD8+T
cells
—
29.4%
(20.7;
39.7)
group,
26.5%
(21.1;
32.7)
21.3%
(17.1;
26.0)
controls
(p
0.024
0.026,
In
elevated
range
(508
(372;
622)
356
(247;
531),
0.044).
Thus,
showed
an
imbalance
CD4+and
level
even
at
6–12
months
SARS-CoV-2
infection,
observed
changes
could
be
closely
related
maturation
differentiation.
Such
long-term
decline
TREC
circulation
may
have
profound
impact
on
immune
system
functions
requires
immunocorrection
therapy.
Viruses,
Год журнала:
2024,
Номер
17(1), С. 41 - 41
Опубликована: Дек. 30, 2024
Background:
During
the
acute
phase
of
COVID-19,
a
number
immunological
abnormalities
have
been
reported,
but
few
studies
longitudinally
analyzed
specific
subsets
peripheral
blood
lymphocytes.
Methods:
In
this
observational,
prospective,
and
longitudinal
study,
adult
patients
developing
pneumonia
during
COVID-19
pandemic
followed
up
for
12
months.
Peripheral
lymphocyte
were
assessed
(with
focus
on
memory
markers)
at
6
time
points
after
disease
onset
until
Results:
A
total
76
with
(characterized
by
prevalently
interstitial
pattern
lung
inflammation)
hospital
admission
(who
completed
12-month
follow-up
period)
recruited
in
study.
They
divided
into
two
groups,
namely
positive
(n
=
31)
negative
45)
SARS-CoV-2
PCR
test.
phase,
general
immunophenotyping
profile
was
comparable
most
parameters
between
these
except
B
cells.
When
T
cells
according
to
expression
markers,
significant
decrease
naïve
CD8+
observed
SARS-CoV-2-positive
group
phase.
Notably,
aspect
maintained
period
least
9
Conclusions:
seems
be
associated
lower
compared
virus.
This
alteration
can
persist
convalescent
