Immunological mechanisms of the nucleocapsid protein in COVID-19
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Фев. 14, 2024
The
emergence
of
corona
virus
disease
2019
(COVID-19),
resulting
from
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
has
left
an
indelible
mark
on
a
global
scale,
causing
countless
infections
and
fatalities.
This
investigation
delves
into
the
role
SARS-CoV-2
nucleocapsid
(N)
protein
within
HEK293
cells,
shedding
light
its
influence
over
apoptosis,
interferon
signaling,
cytokines
production.
N
gene
was
amplified,
inserted
pAdTrack-CMV
vector,
then
transfected
to
cells.
Changes
in
expression
IRF3,
IRF7,
IFN-β,
BAK,
BAX,
BCL-2
genes
were
evaluated.
levels
proinflammatory
IL-6,
IL-12,
IL-1β,
TNF-α
also
determined.
exhibited
anti-apoptotic
effect
by
modulating
critical
associated
with
including
BCL-2.
potentially
prolonged
survival
infected
played
immune
evasion
suppressing
pathway,
evidenced
downregulation
essential
regulatory
factors
IRF3
IFN-β
expression.
led
substantial
increase
production
TNF-α.
emerged
as
versatile
factor
exerted
cytokine
These
findings
carry
potential
implications
for
development
targeted
therapies
combat
COVID-19
mitigate
health
impact.
Язык: Английский
Predictive biomarkers of COVID-19 prognosis identified in Bangladesh patients and validated in Japanese cohorts
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Июнь 3, 2024
Abstract
Despite
high
vaccination
rates
globally,
countries
are
still
grappling
with
new
COVID
infections,
and
patients
diagnosed
as
mild
dying
at
home
during
outpatient
treatment.
Hence,
this
study
aim
to
identify,
then
validate,
biomarkers
that
could
predict
if
newly
infected
COVID-19
would
subsequently
require
hospitalization
or
recover
safely
medication
outpatients.
Serum
cytokine/chemokine
data
from
129
within
7
days
after
the
onset
of
symptoms
in
Bangladesh
were
used
training
data.
The
majority
Omicron
variant
over
88%
vaccinated.
Patients
divided
into
those
who
recovered,
deteriorated
moderate
severe
illness.
Using
Lasso
method,
15
predictive
markers
identified
classify
these
two
groups.
validated
a
cohort
194
Covid
Japan
accuracy
exceeded
80%
for
Delta
variants,
70%
Wuhan
Alpha
variants.
In
an
environment
widespread
vaccination,
help
medical
practitioners
determine
will
improve
can
be
managed
on
out-patient
basis,
they
deteriorate
hospitalization.
Язык: Английский
Interplay between Multisystem Inflammatory Syndrome in Children, Interleukin 6, Microbiome, and Gut Barrier Integrity
Immuno,
Год журнала:
2024,
Номер
4(3), С. 226 - 246
Опубликована: Авг. 18, 2024
A
severe
consequence
of
SARS-CoV-2
infection
that
manifests
as
systemic
inflammation
and
multi-organ
involvement
is
called
Multisystem
Inflammatory
Syndrome
in
Children
(MIS-C).
This
review
examines
the
possible
relationship
between
gut
barrier
integrity,
microbiome,
dysregulation
interleukin
6
(IL-6)
signaling,
MIS-C.
Clinical
biochemical
features
MIS-C
are
comparable
to
those
other
hyper-inflammatory
syndromes,
suggesting
a
dysregulated
immune
response.
One
explanation
for
seen
patients
SARS-CoV-2-induced
IL-6
signaling
pathway.
In
addition,
new
data
suggest
reciprocal
link
integrity
IL-6.
exhibits
bacteriophage-like
behavior,
highlighting
role
bacteria
reservoir
virus
emphasizing
importance
understanding
bacteriophagic
mechanism
fecal–oral
transmission.
The
increased
translocation
viral
products
bacterial
toxins
may
result
from
disrupting
intestinal
cause
inflammation.
On
hand,
can
weaken
barrier,
which
feeds
back
into
loop
immunological
dysregulation.
context
MIS-C,
interaction
infection,
IL-6,
shed
light
on
etiology
disease
guide
treatment
options.
Since
children
with
dysbiosis
be
more
susceptible
it
critical
reinforce
their
microbiome
through
probiotics
supplementation,
plant-fiber-rich
diets
(prebiotics).
Early
antibiotic
use
zonulin
antagonists
should
also
considered.
Язык: Английский
Detrimental Effects of Anti-Nucleocapsid Antibodies in SARS-CoV-2 Infection, Reinfection, and the Post-Acute Sequelae of COVID-19
Pathogens,
Год журнала:
2024,
Номер
13(12), С. 1109 - 1109
Опубликована: Дек. 15, 2024
Antibody-dependent
enhancement
(ADE)
is
a
phenomenon
in
which
antibodies
enhance
subsequent
viral
infections
rather
than
preventing
them.
Sub-optimal
levels
of
neutralizing
individuals
infected
with
dengue
virus
are
known
to
be
associated
severe
disease
upon
reinfection
different
serotype.
For
Severe
Acute
Respiratory
Syndrome
Coronavirus
type-2
infection,
three
types
ADE
have
been
proposed:
(1)
Fc
receptor-dependent
infection
cells
expressing
receptors,
such
as
macrophages
by
anti-spike
antibodies,
(2)
receptor-independent
epithelial
and
(3)
cytokine
production
anti-nucleocapsid
antibodies.
This
review
focuses
on
the
induced
examining
its
potential
role
COVID-19
during
contribution
post-acute
sequelae
COVID-19,
i.e.,
prolonged
symptoms
lasting
at
least
months
after
acute
phase
disease.
We
also
discuss
protective
effects
recently
identified
that
neutralize
Omicron
variants.
Язык: Английский
SARS-CoV-2 specific adaptations in N protein inhibit NF-κB activation and alter pathogenesis
The Journal of Cell Biology,
Год журнала:
2024,
Номер
224(1)
Опубликована: Дек. 16, 2024
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
and
severe
(SARS-CoV)
exhibit
differences
in
their
inflammatory
responses
pulmonary
damage,
yet
the
specific
mechanisms
remain
unclear.
Here,
we
discovered
that
SARS-CoV-2
nucleocapsid
(N)
protein
inhibits
activation
of
nuclear
factor-κB
(NF-κB)
pathway
downstream
signal
transduction
by
impeding
assembly
transforming
growth
factor
β-activated
kinase1
(TAK1)–TAK1
binding
2/3
(TAB2/3)
complex.
In
contrast,
SARS-CoV
N
does
not
impact
NF-κB
pathway.
By
comparing
amino
acid
sequences
proteins,
identified
Glu-290
Gln-349
as
critical
residues
C-terminal
domain
(CTD)
protein,
essential
for
its
antagonistic
function.
These
findings
were
further
validated
a
trans-complementation
system
using
cellular
animal
models.
Our
results
reveal
distinctions
triggered
SARS-CoV,
highlighting
significance
alterations
influencing
viral
pathogenicity.
Язык: Английский
T-Cell Phenotypes and Systemic Cytokine Profiles of People Living with HIV Admitted to Hospital with COVID-19
Microorganisms,
Год журнала:
2024,
Номер
12(11), С. 2149 - 2149
Опубликована: Окт. 25, 2024
Whether
SARS-CoV-2
infection
leads
to
a
higher
mortality
and
morbidity
in
people
living
with
HIV
(PLWH)
Africa
remains
inconclusive.
In
this
study,
we
explored
the
differences
T-cell
phenotypes
between
without
on
day
of
admission
(V1)
±7
days
later
(V2),
as
well
their
cytokine/chemokine
profiles
V1.
Patients
admitted
COVID-19
were
recruited
May
2020
December
2021
from
Steve
Biko
Academic
Tshwane
District
Hospitals
Pretoria,
South
Africa.
Of
174
patients,
37
(21%)
PLWH.
determined
by
flow
cytometry,
cytokine
levels
using
multiplex
suspension
bead
array.
PLWH
significantly
younger
than
those
HIV,
more
likely
be
female.
an
adjusted
analysis,
had
percentages
CD4+
central
memory
(CM)
programmed
cell
death
protein
1
(PD-1)+,
CD8+
effector
(EM)2,
EM4
CD57+
cells,
concentrations
interleukin
(IL)-35
at
admission.
counts
>200
cells/mm
Язык: Английский