Akkermansia muciniphila : A promising probiotic against inflammation and metabolic disorders

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 27, 2024

Metabolic disease is a worldwide epidemic that has become public health problem. Gut microbiota considered to be one of the important factors maintain human by regulating host metabolism. As an abundant bacterium in gut,

Язык: Английский

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Gut microbial community and fecal metabolomic signatures in different types of osteoporosis animal models

Aging, Год журнала: 2024, Номер 16(2), С. 1192 - 1217

Опубликована: Янв. 26, 2024

Background: The gut microbiota (GM) constitutes a critical factor in the maintenance of physiological homeostasis. Numerous studies have empirically demonstrated that GM is closely associated with onset and progression osteoporosis (OP). Nevertheless, characteristics its metabolites related to different forms OP are poorly understood. In present study, we examined changes various types as well correlations among them. Methods: We simultaneously established rat postmenopausal, disuse-induced, glucocorticoid-induced models. used micro-CT histological analyses observe bone microstructure, three-point bending tests measure strength, enzyme-linked immunosorbent assay (ELISA) evaluate biochemical markers turnover three models control. applied 16s rDNA analyze abundance employed untargeted metabolomics identify fecal all four treatment groups. implemented multi-omics methods explore relationships OP, GM, metabolites. Results: 16S sequencing revealed both alterations significantly differed postmenopausal model, bacterial genera g__Bacteroidetes_unclassified, g__Firmicutes_unclassified, g__Eggerthella had changed. disuse-induced models, g__Akkermansia g__Rothia changed, respectively. Untargeted disclosed GM-derived types. However, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis showed it was mainly implicated lipid amino acid metabolism were altered cases. An association indicated histidine intermediate 4-(β-acetylaminoethyl) imidazole common strongly correlated metabolism-related genera. Hence, might play vital role progression. Conclusions: work OP. It also characteristic each type Future research should endeavor determine causal regulatory effects typical form

Язык: Английский

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Research trends on the gut microbiota in endocrine metabolism: a thematic and bibliometric analysis

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Март 22, 2024

Background Gut microbiota studies in the field of endocrinology metabolism have attracted increasing attention recent years. To comprehensively assess evolving landscape this research field, we conducted a thorough bibliometric analysis gut indexed Web Science database. Methods We collected and analyzed 3,339 original articles reviews published from 1972 to 2023. Using various indicators, investigated publication trends, country contributions, international collaborations, prolific authors, top journals, influential articles. Results Our revealed significant upsurge publications after 2010, indicating growing scientific interest metabolism. Keyword thematic analyses identified microbiota, obesity, diabetes, inflammation as core themes. Additionally, roles probiotics prebiotics are increasingly researched for their therapeutic effects shaping microbiota. Conclusion This study reveals that is decoding connection between diseases. There’s also focus on manipulation, which points shift towards personalized medicine. Future should integrating these findings into clinical practice, moving lab-based real-world patient care.

Язык: Английский

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The Role of Akkermansia muciniphila on Improving Gut and Metabolic Health Modulation: A Meta-Analysis of Preclinical Mouse Model Studies

Microorganisms, Год журнала: 2024, Номер 12(8), С. 1627 - 1627

Опубликована: Авг. 9, 2024

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Characterization and Anti-Inflammatory Effects of Akkermansia muciniphila-Derived Extracellular Vesicles

Microorganisms, Год журнала: 2025, Номер 13(2), С. 464 - 464

Опубликована: Фев. 19, 2025

Bacterial extracellular vesicles (EVs) play a pivotal role in host-microbe communication. Akkermansia muciniphila, symbiotic bacterium essential for intestinal health, is hypothesized to exert its effects via EVs. Here, we successfully isolated and characterized EVs derived from A. muciniphila (Am-EVs) using ultracentrifugation. Am-EVs exhibited double-membrane structure, with an average diameter of 92.48 ± 0.28 nm proteomic profile comprising 850 proteins. In vitro model lipopolysaccharide (LPS)-induced inflammation human colorectal adenocarcinoma cells (Caco-2), treatment both 25 50 μg/mL significantly reduced oxidative stress markers, including reactive oxygen species (ROS), nitric oxide (NO), malondialdehyde (MDA), while restoring catalase activity (CAT). also suppressed the expression pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6). Subsequent transcriptomic sequencing Western blot experiments revealed that attenuate MAPK signaling pathway by downregulating TRIF, MyD88, p38 MAPK, FOS upregulating TGFBR2. These findings suggest mediate anti-inflammatory through modulation signaling, highlighting their potential as therapeutic agents inflammation.

Язык: Английский

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Bicarbonate-Rich Mineral Water Mitigates Hypoxia-Induced Osteoporosis in Mice via Gut Microbiota and Metabolic Pathway Regulation

Nutrients, Год журнала: 2025, Номер 17(6), С. 998 - 998

Опубликована: Март 12, 2025

Background: High-altitude hypoxia is known to adversely affect bone health, leading accelerated loss and metabolic alterations. Recent studies suggest that factors such as bicarbonate gut microbiota may play key roles in health. Mineral water, rich bicarbonate, influence health the gut–bone axis under conditions. Methods: Mice were exposed treated with different concentrations of drinking water. Bone-related parameters assessed using dual-energy X-ray absorptiometry (DXA) Micro-CT. Bone was measurement serum biomarkers. Additionally, Untargeted Metabolomics employed analyze differential metabolites between groups, while composition analyzed 16S rRNA sequencing. Results: BMW consumption increased mineral density (BMD) helped alleviate damage microstructure bones caused by delayed progression osteoporosis. shown enhance probiotics Akkermansia Dubosiella regulate longevity-regulating pathway well PI3K/AKT/mTOR (PAM) signaling pathway. This study also discovered changes products due intervention, predominantly pathways amino acid, prostaglandin, purine metabolisms, correlation analysis further exploring relationships these metabolites. Conclusions: Long-term exposure high-altitude hypoxic conditions affects structure metabolism mice. The improves regulates maintain hypoxia.

Язык: Английский

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Amuc_1434 From Akkermansia muciniphila Enhances CD8+ T Cell‐Mediated Anti‐Tumor Immunity by Suppressing PD‐L1 in Colorectal Cancer

The FASEB Journal, Год журнала: 2025, Номер 39(8)

Опубликована: Апрель 15, 2025

ABSTRACT Colorectal cancer (CRC) shows a limited response to programmed death‐ligand 1 (PD‐L1) immunotherapies. Akkermansia muciniphila (AKK) may enhance tumor immunity. This study examines how its Outer Membrane Vesicles (OMVs) and Amuc_1434 influence PD‐L1 expression CD8+ T cell activity in CRC. OMVs were isolated their characteristics examined through transmission electron microscopy Western blotting. was quantified via blot, while proliferation measured using flow cytometry. Cytokine production (interferon‐gamma (IFN‐γ) interleukin‐2 (IL‐2)) evaluated ELISA. A CRC mouse model employed examine impact on growth immune infiltration. In cells, treatment with AKK‐derived (AKK‐OMVs) significantly downregulated ( p < 0.05) markedly increased the levels of IFN‐γ IL‐2 0.01). identified as key protein mediating these effects. vivo, AKK‐OMVs substantially reduced volume 0.01) enhanced infiltration into microenvironment Additionally, AKK‐OMVs‐treated mice showed activation markers within tissue, further indicating antitumor reveals that AKK‐OMVs, particularly those containing Amuc_1434, can modulate potentiate cell‐mediated immunity These findings suggest novel approach overcoming resistance checkpoint inhibitors

Язык: Английский

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Effect of Barley and Oat Consumption on Immune System, Inflammation and Gut Microbiota: A Systematic Review of Randomized Controlled Trials

Current Nutrition Reports, Год журнала: 2024, Номер 13(3), С. 582 - 597

Опубликована: Май 24, 2024

Abstract Purpose of Review The aim this systematic review was to investigate the effects whole grain Avena sativa and Hordeum vulgare L., or their isolated fractions, on immune inflammatory functions, as well influence gut microbiota. A structured literature search undertaken in line with PRISMA guidelines. Randomized controlled trials (RCTs) that investigated oats barley consumption adults reported ≥ 1 following: C-reactive protein (CRP), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), IL-2, IL-8, IL-18, lipopolysacharide binding (LBP) microbiota-related outcomes, were included. Recent Findings total 16 RCTs included, among which 6 studies recruited metabolically at-risk population, including individuals overweight obesity, metabolic syndrome hypercholesterolemia. Additionally, 3 involved young healthy 5 targeted older (aged over 50 years), 2 encompassed populations other disease states. 1091 included evaluation short-term (up 14 days) long-term (beyond days, up 90 supplementation barley-based products. 9 measured biomarkers them significant reductions, specifically studies. Notably, no evidence anti-inflammatory benefits found individuals, whereas involving showed promising reductions inflammation. 13 impact microbiota, collectively suggest food products can composition associated some cases improvements. Summary Oats may confer microbiota outcomes. However, observed individuals. Results from suggests caution interpreting findings due limited variations interventions health conditions.

Язык: Английский

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Gut microbiota promotes macrophage M1 polarization in hepatic sinusoidal obstruction syndrome via regulating intestinal barrier function mediated by butyrate

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Июль 16, 2024

Background The intestinal-liver axis is associated with various liver diseases. Here, we verified the role of gut microbiota and macrophage activation in progression pyrrolizidine alkaloids-induced hepatic sinusoidal obstruction syndrome (PA-HSOS), explored possible mechanisms new treatment options.

Язык: Английский

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Repetitive Transcranial Magnetic Stimulation (rTMS) Improves Cognitive Impairment and Intestinal Microecological Dysfunction Induced by High-Fat Diet in Rats

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Consuming a high-fat diet (HFD) is widely recognized to cause obesity and result in chronic brain inflammation that impairs cognitive function. Repetitive transcranial magnetic stimulation (rTMS) has shown effectiveness both weight loss improvement, although the exact mechanism still unknown. Our study examined effects of rTMS on intestinal microecological dysfunction. successfully reduced decline caused by an HFD behavioral assessments involving Y maze novel object recognition. This was accompanied increase number new neurons transcription level genes related synaptic plasticity (spindlin 1, synaptophysin, postsynaptic protein-95) hippocampus. It reached decreased release high mobility group box activation microglia, brains rats. also hypothalamic hypocretin levels improved peripheral blood lipid metabolism. In addition, recovered HFD-induced gut microbiome imbalances, metabolic disorders, and, particular, microvirus. research emphasized enhanced abilities, resulting positive impacts inflammation, neurodegeneration, microbiota gut, indicating potential connection between proposing could be approach addressing deficits linked obesity.

Язык: Английский

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