Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Дек. 9, 2024
Colorectal cancer (CRC) is a leading cause of cancer-related deaths globally. The heterogeneity the tumor microenvironment significantly influences patient prognosis, while diversity cells shapes its unique characteristics. A comprehensive analysis molecular profile crucial for identifying novel targets drug sensitivity and uncovering pathophysiological mechanisms underlying CRC.
Язык: Английский
Macromolecules, Год журнала: 2024, Номер 57(6), С. 2858 - 2867
Опубликована: Март 14, 2024
Aliphatic polycarbonates (APCs) are promising biocompatible and degradable polymers with immense potential for biomedical applications. However, current synthetic approaches to stimuli-responsive APCs remain limited often complicated, requiring multiple-step postpolymerization reactions. To address these limitations unlock the full of in development advanced biomaterials, we developed a rapid versatile strategy synthesize glutathione (GSH)-responsive through controlled ring-opening polymerization cyclic carbonates activated disulfides. Responsive pendant moieties could be easily introduced one step highly efficient thiol–disulfide exchange reaction enabled by reactive pyridyl disulfides, providing simple approach fine-tuning polymer's physiochemical properties As proof concept, prepared an amphiphilic GSH-responsive polycarbonate-drug conjugate initiating carbonate monomer methyl polyethylene glycol then conjugating anticancer drug mertansine via reaction. The self-assembled nanoparticles were smart delivery system GSH-triggered release specifically within cancer cells remarkable selective toxicity toward over healthy cells, showcasing exceptional promise this polycarbonate
Язык: Английский
Процитировано
10
Advanced Science, Год журнала: 2024, Номер 11(30)
Опубликована: Июнь 12, 2024
Senescent cancer cells are endowed with high immunogenic potential that has been leveraged to elicit antitumor immunity and potentially complement anticancer therapies. However, the efficacy of live senescent cell-based vaccination is limited by interference from immunosuppressive senescence-associated secretory phenotype pro-tumorigenic capacity cells. Here, a nanovaccine strong immunogenicity favorable for immunotherapy reported. The biomimetic integrating cell membrane-coated nanoadjuvant outperforms living in enhancing dendritic (DCs) internalization, improving lymph node targeting, immune responses. In contrast nanovaccines generated death-induced tumor cells, facilitate DC maturation, eliciting superior protection therapeutic outcomes melanoma-challenged mice fewer side effects when combined αPD-1. study suggests versatile biomanufacturing approach maximize minimize adverse advances design immunotherapy.
Язык: Английский
Процитировано
9
Frontiers in Immunology, Год журнала: 2025, Номер 15
Опубликована: Янв. 17, 2025
High-grade serous ovarian cancer (HGSOC), the predominant subtype of epithelial cancer, is frequently diagnosed at an advanced stage due to its nonspecific early symptoms. Despite standard treatments, including cytoreductive surgery and platinum-based chemotherapy, significant improvements in survival have been limited. Understanding molecular mechanisms, immune landscape, drug sensitivity HGSOC crucial for developing more effective personalized therapies. This study integrates insights from immunology, profiling, analysis identify novel therapeutic targets improve treatment outcomes. Utilizing single-cell RNA sequencing (scRNA-seq), systematically examines tumor heterogeneity microenvironment, focusing on biomarkers influencing response activity, aiming enhance patient outcomes quality life. scRNA-seq data was obtained GEO database this study. Differential gene expression analyzed using ontology set enrichment methods. InferCNV identified malignant cells, while Monocle, Cytotrace, Slingshot software inferred differentiation trajectories. The CellChat package predicted cellular communication between cell subtypes other pySCENIC utilized transcription factor regulatory networks within subtypes. Finally, results were validated through functional experiments, a prognostic model developed assess prognosis, infiltration, across various risk groups. investigated scRNA-seq, their interactions microenvironment. We confirmed key role C2 IGF2+ HGSOC, which significantly associated with poor prognosis high levels chromosomal copy number variations. located terminal tumor, displaying higher degree malignancy close association IIIC tissue types. also metabolic pathways, such as glycolysis riboflavin metabolism, well programmed death processes. highlighted complex fibroblasts MK signaling pathway, may be closely related tumor-associated progression. Elevated PRRX1 connected impact disease progression by modulating transcription. A based demonstrated adverse outcomes, emphasizing importance infiltration clinical intervention strategies. oncology, immunotherapy, reveal mechanisms driving resistance. subtype, linked offers promising target future Emphasizing sensitivity, research highlights strategies life patients.
Язык: Английский
Процитировано
1
Advanced Functional Materials, Год журнала: 2024, Номер 34(44)
Опубликована: Май 25, 2024
Abstract The vigorous development of cancer nanomedicine has revolutionized traditional oncology medicine, but it is also limited by the continuous mutation cunning cells, leading to apoptosis insensitivity and therapeutic disappointment. Inflammatory‐regulated cell death (RCD), especially pyroptosis‐related death, demonstrates huge potential for sensitization due its unique biochemical characteristics. aim this research present a thorough synopsis current knowledge on pyroptosis‐associated inflammatory including pyroptosis, cuproptosis, PANoptosis, synergistic function in nano therapy. Paradigm studies death‐mediated apoptosis‐sensitizing tumor nanotherapeutics are introduced detail, coordination mechanisms based nanomaterials discussed. In addition, multi‐angle analysis future prospects pyroptosis‐sensitized various emphasized further expand application scope RCD. It believed that emerging auxiliary treatments RCD will greatly promote progress nanomedicine.
Язык: Английский
Процитировано
7
Advanced Science, Год журнала: 2024, Номер 11(17)
Опубликована: Фев. 29, 2024
Abstract Universal protein coatings have recently gained wide interest in medical applications due to their biocompatibility and ease of fabrication. However, the challenge persists activity preservation, significantly complicating functional design these coatings. Herein, an active dual‐protein surface engineering strategy assisted by a facile stepwise protein–protein interactions assembly (SPPIA) method for catheters reduce clot formation infection is proposed. This realized first partial oxidation bovine serum albumin (BSA) lysozyme (LZM) creating stable nucleation platforms via hydrophobic interaction, followed nonoxidized BSA (pI, isoelectric point, ≈4.7) LZM (pI ≈11) through electrostatic interaction owing opposite charge under neutral conditions. The SPPIA effectively preserves conformation functionality both LZM, thus endowing resultant coating with potent antithrombotic bactericidal properties. Furthermore, platform ensures adhesion durability coating, resisting thrombosis bacterial proliferation even after 15 days PBS immersion. Overall, approach not only provides new fabrication but also shows promise technology catheters.
Язык: Английский
Процитировано
6
Research, Год журнала: 2024, Номер 7
Опубликована: Янв. 1, 2024
Achieving antitumor immunotherapy based on hybridization of multiple types inactivated cells has attracted a lot attention. However, the hybridized disordered structure could result in shedding antigens and their transfer to immune cells, which suppresses tumor immunity through trogocytosis. Here, we report strategy for situ solidification whole cell by biomineralization sustained stimulation immunity. The near-infrared light was used accelerate breaking Au=P bonds auranofin, exposed Au atoms biomineralize at secondary (β-corner) protein form nanocrystals with coronas cells. are anchored coronas, fixes morphology rendering them physiologically inactive. Interestingly, this solidified prevents from undergoing trogocytosis, inhibits proximal distal growth. Thus, study presents its potential application immunotherapy.
Язык: Английский
Процитировано
5
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(18)
Опубликована: Апрель 4, 2024
The major bottleneck in using polymer nanovectors for biomedical application, particularly those based on self-immolative poly(amino ester) (PAE), lies their uncontrolled autodegradation at physiological pH before they can reach the intended target. Here, an elegant triblock-copolymer strategy is designed to stabilize unstable PAE chains via zwitterionic interactions under (pH 7.4) and precisely program enzyme-responsive biodegradation specifically within intracellular compartments, ensuring targeted delivery of cargoes. To achieve this goal, biodegradable polycaprolactone (PCL) platform chosen, structure-engineered several di- triblock architectures arrive precise macromolecular geometry. hydrophobic-PCL core hydrophilic anionic-PCL block periphery shield PAEs against autodegradation, thereby stability PBS, FBS, cell culture medium bloodstream. clinical anticancer drug doxorubicin deep-tissue penetrable near-infrared IR-780 biomarker encapsulated study biological actions by vitro live cancer cells vivo bioimaging animals. These zwitterions are biocompatible, nonhemolytic, real-time live-cell confocal studies have confirmed internalization enzymatic endo-lysosomal compartments deliver payload. In establishes prolonged blood circulation over 72 h, biodistribution analysis reveals accumulation nanoparticles predominantly excretory organs.
Язык: Английский
Процитировано
5
Frontiers in Oncology, Год журнала: 2025, Номер 14
Опубликована: Янв. 21, 2025
Hepatocellular carcinoma (HCC) is one of the most common lethal cancers worldwide. Natural killer cells (NK cells) play a key role in liver immunosurveillance, but tumor microenvironment, NK are readily depleted, as evidenced by down-regulation activating receptors, reduced cytokine secretion, and attenuated killing function. The up-regulation inhibitory such PD-1, TIM-3, LAG-3, further exacerbates depletion cells. Combined blockade strategies targeting these immunosuppressive mechanisms, combination PD-1 inhibitors with other pathways (eg. TIM-3 LAG-3), have shown potential to reverse cell exhaustion preclinical studies. This article explores promise innovative HCC immunotherapy, providing new therapeutic directions for optimizing function improving drug sensitivity.
Язык: Английский
Процитировано
0
Translational Oncology, Год журнала: 2025, Номер 54, С. 102331 - 102331
Опубликована: Фев. 28, 2025
Язык: Английский
Процитировано
0
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Март 24, 2025
Cell membrane coating has emerged as a promising strategy for the surface modification of biomaterials with biological membranes, serving cloak that can carry more functions. The cloaked inherit diverse intrinsic biofunctions derived from different cell sources, including enhanced biocompatibility, immunity evasion, specific targeting capacity, and immune regulation regenerative microenvironment. characteristics biomimicry biointerfacing have demonstrated versatility technology on variety biomaterials, thus, furthering research into wide range biomedical applications clinical translation. Here, preparation coatings is emphasized, sizes coated nanoscale to macroscale well engineering strategies introduce additional are summarized. Subsequently, utilization biomimetic membrane-cloaked in discussed, drug delivery, imaging phototherapy, cancer immunotherapy, anti-infection detoxification, implant modification. In conclusion, latest advancements preclinical studies, along multiple benefits membrane-coated nanoparticles (NPs) systems, elucidated.
Язык: Английский
Процитировано
0