A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Год журнала: 2021, Номер 10

Опубликована: Окт. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Язык: Английский

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Transforming representations of movement from body- to world-centric space

Nature, Год журнала: 2021, Номер 601(7891), С. 98 - 104

Опубликована: Дек. 15, 2021

Язык: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Год журнала: 2024, Номер 634(8032), С. 139 - 152

Опубликована: Окт. 2, 2024

Язык: Английский

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A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

eLife, Год журнала: 2023, Номер 12

Опубликована: Фев. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Язык: Английский

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A functionally ordered visual feature map in the Drosophila brain

Neuron, Год журнала: 2022, Номер 110(10), С. 1700 - 1711.e6

Опубликована: Март 14, 2022

Topographic maps, the systematic spatial ordering of neurons by response tuning, are common across species. In Drosophila, lobula columnar (LC) neuron types project from optic lobe to central brain, where each forms a glomerulus in distinct position. However, advantages this glomerular arrangement unclear. Here, we examine functional and relationships 10 glomeruli using single-neuron calcium imaging. We discover novel detectors for objects smaller than lens resolution (LC18) complex line motion (LC25). find that spatially clustered selectivity looming versus drifting object ordered size tuning form topographic visual feature map. Furthermore, connectome analysis shows downstream integrate sparse subsets possible combinations, which biased encoding similar features. LC thus an explicit example topographically organized facilitate circuit integration.

Язык: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

BMC Bioinformatics, Год журнала: 2024, Номер 25(1)

Опубликована: Март 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Язык: Английский

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Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain

eLife, Год журнала: 2021, Номер 10

Опубликована: Сен. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Язык: Английский

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Neuronal circuits integrating visual motion information in Drosophila melanogaster

Current Biology, Год журнала: 2022, Номер 32(16), С. 3529 - 3544.e2

Опубликована: Июль 14, 2022

The detection of visual motion enables sophisticated animal navigation, and studies on flies have provided profound insights into the cellular circuit bases this neural computation. fly's directionally selective T4 T5 neurons encode ON OFF motion, respectively. Their axons terminate in one four retinotopic layers lobula plate, where each layer encodes directions motion. Although input circuitry has been studied detail, synaptic connectivity circuits integrating T4/T5 signals is largely unknown. Here, we report a 3D electron microscopy reconstruction, wherein comprehensively identified T4/T5's partners revealing diverse set new cell types attributing patterns to known types. Our reconstruction explains how ON- OFF-motion pathways converge. cells that project same connect common comprise core motif together with bilayer interneurons, detailing basis for computing opponency. We discovered likely by vertical horizontal from upstream neurons. Finally, identify substantial projections lobula, extending suggesting shape feature there. describe enrich anatomical experimental computations analyses vision bring us closer understanding complete sensory-motor pathways.

Язык: Английский

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Synaptic gradients transform object location to action

Nature, Год журнала: 2023, Номер 613(7944), С. 534 - 542

Опубликована: Янв. 4, 2023

Abstract To survive, animals must convert sensory information into appropriate behaviours 1,2 . Vision is a common sense for locating ethologically relevant stimuli and guiding motor responses 3–5 How circuitry converts object location in retinal coordinates to movement direction body remains largely unknown. Here we show through behaviour, physiology, anatomy connectomics Drosophila that visuomotor transformation occurs by conversion of topographic maps formed the dendrites feature-detecting visual projection neurons (VPNs) 6,7 synaptic weight gradients VPN outputs onto central brain neurons. We demonstrate how this gradient motif transforms anteroposterior looming stimulus fly’s directional escape. Specifically, discover two postsynaptic looming-responsive type promote opposite takeoff directions. Opposite these from VPNs different field regions localized threats correctly oriented escapes. For second type, graded along dorsoventral axis. generalizes across all 20 primary cell types most often arises without axon topography. Synaptic may thus be general mechanism conveying spatial features directed outputs.

Язык: Английский

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Visual processing in the fly, from photoreceptors to behavior

Genetics, Год журнала: 2023, Номер 224(2)

Опубликована: Май 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Язык: Английский

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