cFOS as a biomarker of activity maturation in the hippocampal formation

Frontiers in Neuroscience, Год журнала: 2023, Номер 17

Опубликована: Июль 13, 2023

We explored the potential for cFOS expression as a marker of functional development “resting-state” waking activity in extended network hippocampus and entorhinal cortex. examined sleeping awake mice at (P)ostnatal days 5, 9, 13, 17 well adulthood. find that is state-dependent even 5 old, with reliable staining occurring only mice. Even during waking, was rare weak P5. The septal nuclei, cortex layer (L)2, anterodorsal thalamus were exceptional they had robust P5 similar to or greater than Significant also observed dentate gyrus, L6, postsubiculum L4-6, ventral subiculum, supramammillary nucleus, posterior hypothalamic nucleus. these regions grew stronger age, new added progressively P9 P13 by which point overall pattern many qualitatively adult. Six regions—CA1, dorsal L2-3, reuniens perirhinal postrhinal cortices—were very late developing, mostly achieving adult levels after P17. Our findings support number developmental principles. First, early spontaneous patterns induced muscle twitches sleep do not induce hippocampal network. Second, follows progressive activation along trisynaptic circuit, rather birth date cellular maturation. Third, we reveal components egocentric head-direction theta-rhythm circuits earliest active forebrain. results suggest may provide sensitive biomarker formation development, particularly regard attainment normal state synchronizing rhythms such theta gamma.

Язык: Английский

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Somatosensory-Evoked Early Sharp Waves in the Neonatal Rat Hippocampus

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8721 - 8721

Опубликована: Май 13, 2023

The developing entorhinal–hippocampal system is embedded within a large-scale bottom-up network, where spontaneous myoclonic movements, presumably via somatosensory feedback, trigger hippocampal early sharp waves (eSPWs). hypothesis, that feedback links movements with eSPWs, implies direct stimulation should also be capable of evoking eSPWs. In this study, we examined responses to electrical the periphery in urethane-anesthetized, immobilized neonatal rat pups using silicone probe recordings. We found ~33% trials evoked local field potential (LFP) and multiple unit activity (MUA) identical somatosensory-evoked eSPWs were delayed from stimulus, on average, by 188 ms. Both (i) had similar amplitude ~0.5 mV half-duration ~40 ms, (ii) current-source density (CSD) profiles, current sinks CA1 strata radiatum, lacunosum-moleculare DG molecular layer (iii) associated MUA increase DG. Our results indicate can triggered stimulations support hypothesis sensory involved association rats.

Язык: Английский

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Neural decoding reveals specialized kinematic tuning after an abrupt cortical transition

Cell Reports, Год журнала: 2023, Номер 42(9), С. 113119 - 113119

Опубликована: Сен. 1, 2023

The primary motor cortex (M1) exhibits a protracted period of development, including the development sensory representation long before outflow emerges. In rats, this is present by postnatal day (P) 8, when M1 activity "discontinuous." Here, we ask how changes upon transition to "continuous" at P12. We use neural decoding predict forelimb movements from and show that linear decoder effectively predicts limb P8 but not P12; instead, nonlinear better altered performance reflects increased complexity uniqueness kinematic information in M1. next M1's P12 more susceptible "lesioning" inputs "transplanting" encoding scheme one pup another. Thus, emergence continuous signals developmental onset complex, informationally sparse, individualized representations.

Язык: Английский

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Synchronous excitation in the superficial and deep layers of the medial entorhinal cortex precedes early sharp waves in the neonatal rat hippocampus

Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Апрель 26, 2024

Early Sharp Waves (eSPWs) are the earliest pattern of network activity in developing hippocampus neonatal rodents. eSPWs were originally considered to be an immature prototype adult SPWs, which spontaneous top-down hippocampal events that self-generated circuitry. However, recent studies have shifted this paradigm a bottom-up model eSPW genesis, primarily driven by inputs from layers 2/3 medial entorhinal cortex (MEC). A hallmark SPWs is relay information CA1 target structures, including deep EC. Whether and how MEC activated during neonates remains elusive. In study, we investigated layer 5 rat pups using silicone probe recordings hippocampus. We found neurons superficial fire synchronously sharp potentials, neuronal firing both precedes activation eSPWs. Thus, sequence EC waves reverses development, lead SPWs. These findings suggest another important difference generative mechanisms possible functional roles compared

Язык: Английский

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Network instability dynamics drive a transient bursting period in the developing hippocampus in vivo

eLife, Год журнала: 2022, Номер 11

Опубликована: Дек. 19, 2022

Spontaneous correlated activity is a universal hallmark of immature neural circuits. However, the cellular dynamics and intrinsic mechanisms underlying network burstiness in intact developing brain are largely unknown. Here, we use two-photon Ca

Язык: Английский

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Cortical neuronal assemblies coordinate with EEG microstate dynamics during resting wakefulness

Cell Reports, Год журнала: 2023, Номер 42(2), С. 112053 - 112053

Опубликована: Янв. 29, 2023

The disruption of cortical assembly activity has been associated with anesthesia-induced loss consciousness. However, the relationship between and variations in consciousness natural vigilance states remains unclear. Here, we address this by performing state-specific clustering analysis on 2-photon calcium imaging data from sensorimotor cortex combination global electroencephalogram (EEG) microstate derived multi-EEG signals obtained over widespread locations. We report no difference structure during quiet wakefulness (QW), non-rapid eye movement sleep (NREMs), or REMs, despite latter two being significantly reduced levels relative to QW. describe a significant coordination EEG dynamics general local periods QW, but not sleep. These results suggest that brain could be key factor sustained conscious experience.

Язык: Английский

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Cortical interneurons: fit for function and fit to function? Evidence from development and evolution

Frontiers in Neural Circuits, Год журнала: 2023, Номер 17

Опубликована: Май 4, 2023

Cortical inhibitory interneurons form a broad spectrum of subtypes. This diversity suggests division labor, in which each cell type supports distinct function. In the present era optimisation-based algorithms, it is tempting to speculate that these functions were evolutionary or developmental driving force for we see mature mammalian brain. this study, evaluated hypothesis using two most common interneuron types, parvalbumin (PV) and somatostatin (SST) expressing cells, as examples. PV SST control activity bodies apical dendrites excitatory pyramidal respectively, due combination anatomical synaptic properties. But was compartment-specific inhibition indeed function cells originally evolved? Does compartmental structure shape diversification over development? To address questions, reviewed reanalyzed publicly available data on development evolution one hand, morphology other. These speak against idea compartment drove into interneurons. particular, late, while are likely committed particular fate (PV vs. SST) during early development. Moreover, comparative anatomy single RNA-sequencing indicate but not existed last ancestor mammals reptiles. Specifically, turtle songbird also express

Язык: Английский

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Comparison of extracellular Giant Depolarizing Potentials in vitro and Early Sharp Waves in vivo in the CA1 hippocampus of neonatal rats

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 735, С. 150823 - 150823

Опубликована: Окт. 11, 2024

Язык: Английский

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Precise Spiking Motifs in Neurobiological and Neuromorphic Data

Brain Sciences, Год журнала: 2022, Номер 13(1), С. 68 - 68

Опубликована: Дек. 29, 2022

Why do neurons communicate through spikes? By definition, spikes are all-or-none neural events which occur at continuous times. In other words, on one side binary, existing or not without further details, and the other, can any asynchronous time, need for a centralized clock. This stands in stark contrast to analog representation of values discretized timing classically used digital processing base modern-day networks. As systems almost systematically use this so-called event-based living world, better understanding phenomenon remains fundamental challenge neurobiology order interpret profusion recorded data. With growing intelligent embedded systems, it also emerges as new computing paradigm enable efficient operation class sensors computers, called neuromorphic, could significant gains computation time energy consumption—a major societal issue era economy global warming. review paper, we provide evidence from biology, theory engineering that precise plays crucial role our efficiency

Язык: Английский

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Cortical Representation of Movement Across the Developmental Transition to Continuous Neural Activity

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 22, 2023

Summary Primary motor cortex (M1) exhibits a protracted period of development that includes the establishment somatosensory map long before outflow emerges. In rats, sensory representation is established by postnatal day (P) 8 when cortical activity still “discontinuous.” Here, we ask how survives sudden transition to noisy “continuous” at P12. Using neural decoding predict forelimb movements based solely on M1 activity, show linear decoder sufficient limb P8, but not P12; in contrast, nonlinear effectively predicts The change performance P12 reflects an increase both complexity and uniqueness kinematic information available M1. We next more susceptible deleterious effects “lesioning” inputs “transplanting” M1’s encoding scheme from one pup another. conclude emergence continuous signals developmental onset complex, informationally sparse, individualized representations.

Язык: Английский

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