Synovial fluid analysis with compensated polarization light microscopy: a physics approach to quantitative understanding of birefringence, polarization, and Maltese crosses DOI Creative Commons
Tom Niessink, Tim Jansen,

Madé Kleinherenbrink

et al.

Clinical Rheumatology, Journal Year: 2024, Volume and Issue: 43(12), P. 3597 - 3602

Published: Oct. 16, 2024

Language: Английский

Predominance of Calcium Pyrophosphate Crystals in Synovial Fluid Samples of Patients at a Large Tertiary Center DOI Creative Commons
Tobias Manigold, Alexander Leichtle

Diagnostics, Journal Year: 2025, Volume and Issue: 15(7), P. 907 - 907

Published: April 1, 2025

Background: Crystal arthritides represent the most common inflammatory rheumatologic condition. While prevalence of gouty arthritis by monosodium urate (MSU) is well established, prevalences calciumpyrophosphat (CPP) and basic calcium pyrophosphate (ARP) are less clear. We herein sought to assess characteristics crystal at our institution, biggest tertiary center in Switzerland. Methods: A total 5036 synovial fluid (SF) samples were analyzed with regard positivity as joint, age, sex distribution affected patients. furthermore compared non-inflammatory SF for yields their Polymorphonuclear (PMN) fractions. Results: About half all derived from knee joints, a male/female ratio up 10.1:1 among MSU-positive, clear shift towards elder patients CPP-arthritis was seen. These findings line previous studies suggest good comparability cohort. Of note, 21.9% CPP positive, whereas 15.3% 9.5% positive MSU ARP/alizarin-red respectively. Importantly, crystals predominant (58.9%) (65.7%) samples. By contrast, significantly more often associated synovitis (p < 0.001). Interestingly, higher PMN fractions found MSU-positive 0.01), similar trend seen CPP-positive Conclusions: represented frequent form center. Higher subclinical inflammation provide further arguments earlier anti-inflammatory uric acid-lowering therapies deposits.

Language: Английский

Citations

0
Calcium Pyrophosphate Crystal Formation and Deposition: Where Do we Stand and What Does the Future hold? DOI Creative Commons
Silvia Sirotti, Anna Scanu, Tristan Pascart

et al.

Current Rheumatology Reports, Journal Year: 2024, Volume and Issue: 26(10), P. 354 - 365

Published: Aug. 1, 2024

Although calcium pyrophosphate deposition (CPPD) has been known since the 1960s, our understanding of its pathogenesis remains rudimentary. This review aims to illustrate mechanisms underlying (CPP) crystal formation and explore future directions in research. By examining various perspectives, from basic research clinical imaging assessments, as well new emerging methodologies, we can establish a starting point for deeper CPPD pathogenesis.

Language: Английский

Citations

2
An objective diagnosis of gout and calcium pyrophosphate deposition disease with machine learning of Raman spectra acquired in a point-of-care setting DOI Creative Commons
Tom Niessink, Tim Jansen,

Frank A. W. Coumans

et al.

Lara D. Veeken, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Raman spectroscopy is proposed as a next-generation method for the identification of monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals in synovial fluid. As interpretation spectra requires specific expertise, not directly applicable clinicians. We developed an approach to demonstrate that process can be automated with use machine learning techniques. The system tested point-of-care-setting at our outpatient rheumatology department.

Language: Английский

Citations

0
Raman Spectroscopy Reveals Microparticles in Synovial Fluids of Patients With Suspected Implant‐Related Complications DOI Creative Commons
Tom Niessink,

Jorrit W. A. Schoenmakers,

Matthijs Janssen

et al.

Journal of Raman Spectroscopy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

ABSTRACT Prosthetic implant‐associated inflammation and failure can be caused by bacterial infections mechanical wear of the prosthesis. Currently, there is no diagnostic modality that allows simultaneous identification both causes implant failure. Here, we present a proof‐of‐principle study to assess whether Raman spectroscopy applied diagnose Synovial fluids from 10 patients with clinical suspicion implant‐related complications were previously collected cultured determine presence bacteria. The microparticles in these synovial was assessed verified scanning electron microscopy combined energy‐dispersive X‐ray (SEM‐EDX). For control, possibility detect vitro ‐ Staphylococcus aureus investigated. revealed all fluid samples contained microparticles: eight microplastics (polyethylene, polypropylene, polystyrene), nine titanium dioxide nanoparticles (anatase rutile) as SEM‐EDX. There clear difference microparticle content or without signals relating individual bacteria clusters detectable cultures S. , but it not possible demonstrate spectroscopy. potential tool for characterizing complications. This relevance cause joint inflammation. feasible, further research needed before implementation.

Language: Английский

Citations

0
Synovial fluid analysis with compensated polarization light microscopy: a physics approach to quantitative understanding of birefringence, polarization, and Maltese crosses DOI Creative Commons
Tom Niessink, Tim Jansen,

Madé Kleinherenbrink

et al.

Clinical Rheumatology, Journal Year: 2024, Volume and Issue: 43(12), P. 3597 - 3602

Published: Oct. 16, 2024

Language: Английский

Citations

0