Plant Cryopreservation: Principles, Applications, and Challenges of Banking Plant Diversity at Ultralow Temperatures

Annual Review of Plant Biology, Journal Year: 2024, Volume and Issue: 75(1), P. 797 - 824

Published: Jan. 12, 2024

Progressive loss of plant diversity requires the protection wild and agri-/horticultural species. For species whose seeds are extremely short-lived, or rarely never produce seeds, genetic makeup must be preserved, cryopreservation offers only possibility for long-term conservation. At temperatures below freezing, most vegetative tissues suffer severe damage from ice crystal formation require protection. In this review, we describe how increasing concentration cellular solutes by air drying adding cryoprotectants, together with rapid cooling, results in a vitrified, highly viscous state which cells can remain viable stored. On basis, range dormant bud-freezing, slow-cooling, (droplet-)vitrification protocols have been developed, but few used to cryobank important agricultural/horticultural/timber threatened To improve efficiency, effects cryoprotectants molecular processes need understood costs cryobanking reduced. However, overall, low, while benefits huge.

Language: Английский

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3D Bioprinting for Drug Development and Screening: Recent Trends Towards Personalized Medicine

Hybrid Advances, Journal Year: 2024, Volume and Issue: unknown, P. 100320 - 100320

Published: Oct. 1, 2024

Language: Английский

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Recent development in piezoelectric materials and devices for cryogenic environments

Sensors and Actuators A Physical, Journal Year: 2025, Volume and Issue: unknown, P. 116317 - 116317

Published: Feb. 1, 2025

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Current State and Challenges of Tissue and Organ Cryopreservation in Biobanking

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11124 - 11124

Published: Oct. 16, 2024

Recent years have witnessed significant advancements in the cryopreservation of various tissues and cells, yet several challenges persist. This review evaluates current state cryopreservation, focusing on contemporary methods, notable achievements, ongoing difficulties. Techniques such as slow freezing vitrification enabled successful preservation diverse biological materials, including embryos ovarian tissue, marking substantial progress reproductive medicine regenerative therapies. These achievements highlight improved post-thaw survival functionality cryopreserved samples. However, there are remaining ice crystal formation, which can lead to cell damage, larger, more complex organs. also explores role cryoprotectants importance optimizing both cooling warming rates enhance outcomes. Future research priorities include developing new cryoprotective agents, elucidating mechanisms cryoinjury, refining protocols for preserving comprehensive overview underscores transformative potential biomedicine, while emphasizing necessity innovation address existing challenges.

Language: Английский

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Synthesis of trehalose-grafted poly(ester amide) with potential ice recrystallization inhibition activity

Polymer, Journal Year: 2024, Volume and Issue: 296, P. 126819 - 126819

Published: Feb. 23, 2024

Language: Английский

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Optimisation of cryopreservation conditions, including storage duration and revival methods, for the viability of human primary cells

BMC Molecular and Cell Biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: Sept. 30, 2024

Language: Английский

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Current Fertility Preservation Steps in Young Women Suffering from Cancer and Future Perspectives

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4360 - 4360

Published: April 15, 2024

Childhood cancer incidence, especially in high-income countries, has led to a focus on preserving fertility this vulnerable population. The common treatments, such as radiation and certain chemotherapeutic agents, though effective, pose risk fertility. For adult women, established techniques like embryo egg freezing are standard, requiring ovarian stimulation. However, for prepubescent girls, tissue become the primary option, eliminating need hormonal preparation. This review describes beginning, evolution, current situation of preservation options young A total 75 studies were included, covering steps protocols: (i) extraction, (ii) method, (iii) thawing transplantation. Cryopreservation subsequent transplantation have resulted successful restoration, with over 200 recorded live births, including cases involving cryopreserved from girls. Despite promising results, challenges persist, follicular loss during transplantation, which is attributed ischemic oxidative damage. Optimizing tissue-freezing processes exploring alternatives vitro systems follicles establish maturation, essential mitigating associated risks. Further research required enhance clinical outcomes future. Ovarian cryopreservation appears be method specific benefits, indications, risks, can an important tool terms younger women.

Language: Английский

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Engineering a Microfluidic Platform to Cryopreserve Stem Cells: A DMSO‐Free Sustainable Approach

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(29)

Published: Aug. 17, 2024

Abstract Human adipose‐derived stem cells (hASCs) are cryopreserved traditionally using dimethyl sulfoxide (DMSO) as the cryoprotectant agent. DMSO penetrates cell membranes and prevents cellular damage during cryopreservation. However, is not inert to cells, inducing cytotoxic effects by causing mitochondrial dysfunction, reduced proliferation, impaired hASCs transplantation. Additionally, large‐scale production of contamination can adversely impact environment. A sustainable, green alternative trehalose, a natural disaccharide agent that does pose any risk cytotoxicity. permeability trehalose less compared DMSO. Here, microfluidic chip developed for intracellular delivery in hASCs. The designed mechanoporation, which creates transient pores mechanical deformation. Mechanoporation allows sparingly permeable be internalized within cytosol. amount delivered intracellularly quantified optimized based on compatibility functionality. Furthermore, whole‐transcriptome sequencing confirms than 1% all target genes display at least twofold change expression when passed through untreated cells. Overall, results confirm feasibility effectiveness this DMSO‐free cryopreservation

Language: Английский

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Identification of Cell Death Markers in Keratinocytes Due to Cryopreservation with Various DMSO Concentrations

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Cryopreservation of cells is a critical challenge in contemporary cryobiology, particularly the burgeoning field tissue engineering. The method cryopreservation significantly affects quality post-thaw, as are sensitive to stress induced by freezing and thawing, leading physical damage, loss functionality, or cell death. It essential develop protocols that ensure good physiological adherent conditions post-thaw. This study investigates effect on human keratinocytes using intracellular cryoprotective agent dimethyl sulfoxide (DMSO), known for mitigating damage during although its toxicity remains debated. We evaluated with low (1.8% 2.2% v/v) standard (5% 10% concentrations DMSO short-term storage (4 days) at -80°C. Post-thaw, we examined impact process viability, plasma membrane fluidity, identified signs death depending concentration used medium. first systematically examine various viability fluidity context 30% FBS medium, slow cooling, − 80°C temperature, highlighting originality significance our research. research demonstrated lower 2.2%) reduce keratinocyte due increased apoptotic activity cellular stress. In contrast, higher 10%) provide better protection maintain viability. revealed increases concentrations, which may facilitate clustering receptors formation signaling complexes, thereby increasing sensitivity stimuli. Morphological analysis showed lead significant morphological changes apoptosis, while result enlargement shape alteration. Ultrastructural provided detailed insights into internal structure cells, revealing nucleus, mitochondria, presence vesicles around whereas led nuclear damage. These findings have implications improve functionality post-thaw enhance success preservation biomedical applications.

Language: Английский

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The Effect of Cryopreservation on the Bone Healing Capacity of Endothelial Progenitor Cells in a Bone Defect Model

Journal of Orthopaedic Research®, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

ABSTRACT Endothelial progenitor cells (EPCs) have proven to be a highly effective cell therapy for critical‐sized bone defects. Cryopreservation can enable long‐term storage of EPCs, allowing their immediate availability on demand. This study compares the therapeutic potential EPCs before and after cryopreservation in small animal defect model. Five‐millimeter segmental defects were created right femora Fischer 344 rats, followed by stabilization with miniplate screws. The animals received 2 × 10 6 fresh ( n = 7) or cryopreserved 9) delivered gelatin scaffold. Cryopreserved stored 7 days at −80°C prior thawing loading onto Biweekly radiographs taken until euthanized weeks surgery. operated then evaluated using microscopic‐computed tomography (micro‐CT) biomechanical testing. All treated 7/7) 9/9) achieved radiographic union weeks. Animals had statistically significant higher scores p < 0.05) but showed no differences thereafter > 0.05). Micro‐CT analysis between groups volume (BV) BV normalized total 0.05), excellent formation both groups. Finally, there outcomes These results demonstrate that are equivalent healing rat model nonunion.

Language: Английский

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