A Close Cognition of Charged Poly(l-methionine) Derivatives for Antifreeze

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Ice formation poses a significant challenge across various fields, from industrial processes to biological preservation. Developing antifreeze agents and recognizing the mechanism have gained considerable attention. Herein, series of poly(l-methionine) derivatives, poly(S-carboxymethyl-l-methionine sulfonium) (PMetA), poly(S-methyl-l-methionine sulfonium chloride) (PMetM), poly(S-carbamidomethyl-l-methionine (PMetAM), with carboxyl, methyl, acetamide groups, respectively, are synthesized investigated for antifreeze. The relationship between polymer structure ice recrystallization inhibition (IRI) activity is examined, suggesting that zwitterionic PMetA shows highest IRI activity, about 27.0 ± 3.9% at 10 mg mL–1 relative water. Results low-field nuclear magnetic resonance differential scanning calorimetry indicate associated activation energy hydrogen bond breakage. exhibits acceptable cytocompatibility 10.0 good cryoprotective efficiency. This finding provides valuable insight into mechanism, contributing development potent cryoprotectants.

Language: Английский

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Side-Functionalization of Poly(l-methionine) for Ice Control

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Controlling ice growth is crucial during the cryopreservation of cells, but current application small molecules as cryoprotectants still remains a challenge. Inspired by structures natural antifreeze (glyco)proteins, in this work, functionalized poly(l-methionine)s (PMets) are synthesized with different side groups including hydroxyl, threonine-mimetic both methyl and hydroxyl (PMet-MOH), zwitterion carboxyl sulfonium (PMet-COOH), glycerol, trehalose pendants. Results suggest that these PMets tend to self-assemble into 100-300 nm nanoparticles positive charges water. The functional have remarkable influence on their control properties. It supposed PMet-MOH inhibits possibly through adsorption mechanism adjacent groups, whereas trehalose-tethered PMet can restrict diffusion water strongest recrystallization inhibition activity zwitterionic PMet-COOH promotes nucleation obviously. This work offers valuable insight development polypeptides promising biocompatible cryoprotectants.

Language: Английский

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Synthesis and Chemical Recovery of Castor Oil-Based Poly(ester amides) with PE-Like Performance

ACS Omega, Journal Year: 2025, Volume and Issue: 10(12), P. 11755 - 11761

Published: March 20, 2025

Biobased closed-loop recyclable olefin-like materials are environmentally friendly and have promising application prospects. However, such scarce, especially the developed so far limited to polyester materials. Therefore, developing nonpolyester biobased is of significant interest. In this study, a castor oil-based poly(ester amide) (PEA) with polyethylene-like properties recyclability was successfully prepared. The obtained PEA exhibit thermal mechanical comparable polyethylene surpass those previously reported linear amide)s. Notably, can be completely hydrolyzed its precursors, N1,N10-bis(3-hydroxypropyl) decanediamide (DSE) sebacic acid (SA), yields 73.1–75.8 92.8–96.2%, respectively. Further experiments demonstrated that repolymerization recovered DSE SA produced REPEA equivalent original polymer. This study achieves recycling for materials, which offers novel approach design sustainable

Language: Английский

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Development of Macromolecular Cryoprotectants for Cryopreservation of Cells

Macromolecular Rapid Communications, Journal Year: 2024, Volume and Issue: 45(19)

Published: July 16, 2024

Cryopreservation is a common way for long-term storage of therapeutical proteins, erythrocytes, and mammalian cells. For cryoprotection these biosamples to keep their structural integrity biological activities, it essential incorporate highly efficient cryoprotectants. Currently, permeable small molecular cryoprotectants such as glycerol dimethyl sulfoxide dominate in cryostorage applications, but they are harmful cells human health. As acting the extracellular space, membrane-impermeable macromolecular cryoprotectants, which exert remarkable membrane stabilization against cryo-injury easily removed post-thaw, promising candidates with biocompatibility feasibility. Water-soluble hydroxyl-containing polymers poly(vinyl alcohol) polyol-based potent ice recrystallization inhibitors, while polyampholytes, polyzwitterions, bio-inspired (glyco)polypeptides can significantly increase post-thaw recovery reduced damages. In this review, synthetic systematically summarized based on synthesis routes, practical utilities, cryoprotective mechanisms. It provides valuable insight development valid inhibition activity safe cryopreservation

Language: Английский

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