Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 7, 2023
Abstract
Objective:
This
study
aims
to
identify
feature
genes,
pathways,
and
infiltrating
immune
cells
related
the
metabolic
mechanisms
of
cellular
disulfidptosis
in
osteoarthritis
(OA)
through
bioinformatics
analysis.
Method:
Expression
profiles
from
two
Gene
Omnibus
datasets
(GSE207881
GSE98918)
were
analyzed
OA.
The
included
63
12
OA
patients,
respectively,
alongside
control
subjects.
Differential
expression
analysis
was
performed
after
data
preprocessing
using
‘limma’
package
R.
A
co-expression
network
constructed
weighted
gene
(WGCNA),
modules
highly
correlated
with
identified.
functionality
explored
Set
Enrichment
Analysis
(GSEA)
Variation
(GSVA).
Additionally,
protein–protein
interactions
(PPI)
key
genes
GeneMANIA.
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analyses
conducted
on
genes.
Furthermore,
diagnostic
potential
selected
evaluated,
infiltration
performed.
Result:
total
522
differentially
expressed
statistical
significance
GSEA
GSVA
revealed
multiple
significantly
enriched
signaling
such
as
ribosome,
melanogenesis,
leukocyte
transendothelial
migration.
Nine
screened
by
WGCNA,
which
blue
module
(n
=
353)
showed
strongest
positive
correlation
(r
0.78,
p
<
0.05).
Intersection
further
identified
13
hub
Through
PPI
networks
GO
KEGG
analyses,
these
found
be
Notch
pathway,
this
pathway
validated.
area
under
receiver
operating
characteristic
curve
greater
than
0.6,
suggesting
their
biomarkers
for
Immune
cell
that
TUSC3
SOX5
have
a
significant
relationship
type
17
T
helper
(p
0.001).
An
RNA-binding
protein
(RBP)–mRNA
interaction
comprising
68
nodes,
61
RBPs,
7
mRNAs,
271
edges
StarBase
online
database.
Conclusion:
used
techniques
reveal
complex
networks,
unique
interactions,
thereby
providing
insights
into
These
findings
lay
groundwork
future
approaches
diagnosis
therapeutic
intervention.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(17), P. 13332 - 13332
Published: Aug. 28, 2023
Nonalcoholic
fatty
liver
disease
(NAFLD)
is
associated
with
mitochondrial
damage.
Circulating
metabolites
may
be
elevated
in
NAFLD
but
their
associations
damage
not
known.
This
study
aimed
to
assess
the
association
of
key
degree
fibrosis
context
and
nonalcoholic
steatohepatitis
(NASH).
Cross-sectional
analyses
were
performed
on
two
cohorts
biopsy-proven
and/or
NASH
subjects.
The
circulating
metabolite
concentrations
was
assessed
using
linear
regression
analysis.
In
single-center
cohort
subjects
(n
=
187),
mean
age
54.9
±13.0
years,
40.1%
female
86.1%
White.
Type
2
diabetes
(51.3%),
hypertension
(43.9%)
obesity
(72.2%)
prevalent.
Those
high
citrate
had
a
higher
proportion
moderate/significant
(stage
F
≥
2)
(68.4
vs.
39.6%,
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 2, 2024
Experimental
evolution
studies
that
feature
selection
on
life-history
characters
are
a
proven
approach
for
studying
the
of
aging
and
variation
in
rates
senescence.
Recently,
incorporation
genomic
transcriptomic
approaches
into
this
framework
has
led
to
identification
hundreds
genes
associated
with
different
patterns.
However,
our
understanding
specific
molecular
mechanisms
underlying
these
patterns
remains
limited.
Here,
we
incorporated
extensive
metabolomic
profiling
generate
mechanistic
insights
Cytokines and inflammation,
Journal Year:
2024,
Volume and Issue:
20(3), P. 17 - 23
Published: April 11, 2024
Purpose.
Investigation
of
the
clinical
features
and
metabolic
status
blood
lymphocytes
in
children
aged
7-11
years
with
gallbladder
dysfunction
depending
on
presence
or
absence
giardiasis.
Materials
methods.
Children
7–11
(GD)
were
examined
Enzyme
activity
was
determined
by
bioluminescent
method.
Results.
There
are
diarrheal
disorders
more
common
clinic
flatulence
–
for
association
Enlargement
liver
cystic
positive
symptoms
also
characterized
a
background
Significant
changes
NAD(P)-dependent
dehydrogenases
found
dysfunction:
reduced
levels
substrate
flux
at
terminal
glycolysis
reactions
tricarboxylic
acid
cycle,
which
determines
inhibition,
respectively,
anaerobic
aerobic
respiration
cells.
Conclusion.
Found
that
combined
pathology
had
lymphocytes,
while
maintaining
intensity
reference
level
enzyme
activity,
determining
state
respiration.
Independently
from
giardiasis
pentose
phosphate
cycle
characterizes
inhibition
corresponding
macromolecular
synthesis.
Monitoring
ventilation
during
veterinary
anesthesia
is
a
fundamental
component
of
perioperative
care
as
the
ability
to
maintain
proper
may
be
compromised
by
anesthetic
and
analgesic
drugs,
muscle
relaxation,
changes
in
respiratory
drive.
includes
both
subjective
objective
techniques.
Quantitative
assessment
carbon
dioxide
via
capnography
allows
evaluation
metabolism,
pulmonary
perfusion
cardiac
output,
alveolar
gas
exchange,
integrity
equipment,
thereby
preventing
complications
ensuring
patient
well-being.
Capnography
also
essential
certain
physiologic
states,
e.g.,
increased
intracranial
pressure
or
cardiopulmonary
arrest.
Employing
appropriate
monitoring
techniques
maintaining
vigilance
can
ensure
that
anesthetist
provides
optimal
care,
minimizing
complications.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 26, 2024
Abstract
Mitochondria
perform
several
essential
functions
in
order
to
maintain
cellular
homeostasis
and
mitochondrial
metabolism
is
inherently
flexible
allow
correct
function
a
wide
range
of
tissues.
Dysregulated
can
therefore
affect
different
tissues
ways
which
presents
experimental
challenges
understanding
the
pathology
diseases.
System-level
metabolic
modelling
useful
gaining
in-depth
insights
into
tissue-specific
metabolism,
yet
despite
mouse
being
common
model
organism
used
research,
there
currently
no
specific
available.
In
this
work,
building
upon
similarity
between
human
we
have
created
mitoMammal,
genome-scale
that
contains
gene-product
reaction
rules.
MitoMammal
able
metabolism.
To
demonstrate
feature,
using
an
adapted
E-Flux2
algorithm,
first
integrated
proteomic
data
extracted
from
mitochondria
isolated
cardiomyocytes
brown
adipocyte
tissue.
We
then
transcriptomic
vitro
differentiated
adipose
cells
modelled
context
flux
balance
analysis.
all
three
simulations,
mitoMammal
made
mostly
accurate,
some
novel
predictions
relating
energy
adipocytes.
This
demonstrates
its
usefulness
research
cardiac
disease
diabetes
both
contexts.
