ACS Macro Letters,
Journal Year:
2024,
Volume and Issue:
unknown, P. 43 - 50
Published: Dec. 19, 2024
This
Letter
presents
complex
coacervation
between
the
biopolymer
diethylaminoethyl
dextran
hydrochloride
(DEAE-Dex)
and
carbon
dots.
The
formation
of
these
coacervates
was
dependent
on
both
DEAE-Dex
concentration
solution
ionic
strength.
Fluorescence
spectroscopy
revealed
that
blue
fluorescence
dots
unaffected
by
coacervation.
Additionally,
microrheological
studies
were
conducted
to
determine
viscosity
coacervates.
These
coacervates,
formed
through
interaction
nanoparticles
polyelectrolytes,
hold
a
promising
role
for
future
applications
where
combination
optical
properties
from
encapsulation
via
can
be
leveraged.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 3, 2025
The
assembly
of
protocells
into
prototissues
represents
a
significant
step
forward
in
mimicking
the
hierarchical
structure
and
complex
functions
biological
tissues.
This
review
explores
construction
various
protocell
models,
including
lipid
vesicles,
polymer
proteinosomes,
membraneless
coacervates,
emulsion
droplets.
It
examines
range
chemical
physical
methods
employed
to
spatially
organize
these
structured,
functional
prototissues.
Furthermore,
highlights
distinctive
properties
prototissues,
such
as
their
ability
facilitate
intra-
inter-protocell
communication,
exhibit
collective
behaviors
like
oscillations
deformation,
promising
applications
biomedicine.
By
bridging
gap
between
synthetic
cells
tissue-like
systems,
this
provides
insights
design,
fabrication,
potential
biology
regenerative
medicine.
The EuroBiotech Journal,
Journal Year:
2024,
Volume and Issue:
8(1), P. 55 - 64
Published: Jan. 1, 2024
Abstract
Artificial
cells
are
synthetic
constructs
that
mimic
the
architecture
and
functions
of
biological
cells.
designed
to
replicate
fundamental
principles
systems
while
also
have
ability
exhibit
novel
features
functionalities
not
been
achieved
before.
Mainly,
made
up
a
basic
structure
like
cell
membrane,
nucleus,
cytoplasm
cellular
organelles.
Nanotechnology
has
used
make
substances
possess
accurate
performance
in
these
structures.
There
many
roles
artificial
can
play
such
as
drug
delivery,
bio-sensors,
medical
applications
energy
storage.
An
additional
prominent
facet
this
technology
is
interaction
with
systems.
The
possibility
being
compatible
living
organisms
opens
potential
for
interfering
specific
activities.
This
element
one
key
areas
research
medicine,
aimed
at
developing
therapies
comprehending
life
processes.
Nevertheless,
exempt
from
ethical
safety
concerns.
interplay
between
structures
may
give
rise
questions
regarding
their
controllability
safety.
Hence,
pursuit
seeks
reconcile
concerns
advantages
technology.
ChemBioChem,
Journal Year:
2024,
Volume and Issue:
25(18)
Published: June 21, 2024
Abstract
Scientific
advancements
in
bottom‐up
synthetic
biology
have
led
to
the
development
of
numerous
models
cells,
or
protocells.
To
date,
research
has
mainly
focused
on
increasing
(bio)chemical
complexity
these
bioinspired
micro‐compartmentalized
systems,
yet
successful
integration
protocells
with
living
cells
remains
one
major
challenges
biology.
In
this
review,
we
aim
summarize
current
state
art
hybrid
protocell/living
cell
and
prototissue/living
systems.
Inspired
by
recent
breakthroughs
tissue
engineering,
review
chemical,
bio‐chemical,
mechano‐chemical
aspects
that
hold
promise
for
achieving
an
effective
non‐living
matter.
The
future
production
fully
integrated
systems
increasingly
complex
not
only
potential
revolutionize
field
but
also
paves
way
new
technologies
(bio)sensing,
personalized
therapy,
drug
delivery.
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 23, 2024
Abstract
The
design
and
implementation
of
collective
actions
in
model
protocell
communities
is
an
on‐going
challenge
synthetic
protobiology.
Herein,
we
covalently
graft
alginate
or
chitosan
onto
the
outer
surface
semipermeable
enzyme‐containing
silica
colloidosomes
to
produce
hairy
catalytic
protocells
with
pH‐switchable
membrane
charge.
Binary
populations
enzymatically
active
exhibit
self‐initiated
stimulus‐responsive
changes
spatial
organization
such
that
mixed
community
undergoes
alternative
modes
electrostatically
induced
self‐sorting
reversible
co‐clustering.
We
demonstrate
co‐clustering,
but
not
self‐sorting,
mitigates
signal
attenuation
a
binary
sender
receiver
due
increased
proximity
effects.
level
correlated
time‐dependent
pH‐mediated
switch
populations.
Our
results
pave
way
towards
development
programmable
networks
adaptive
life‐like
objects
could
have
implications
for
interactive
cytomimetic
materials
agent‐based
robotics.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(13), P. 15993 - 16002
Published: March 20, 2024
Biomaterials
capable
of
delivering
therapeutic
proteins
are
relevant
in
biomedicine,
yet
their
manufacturing
relies
on
centralized
chains
that
pose
challenges
to
remote
implementation
at
the
point
care.
This
study
explores
viability
confined
cell-free
protein
synthesis
within
porous
hydrogels
as
biomaterials
dynamically
produce
and
deliver
vitro
vivo
biological
microenvironments.
These
functional
have
potential
be
assembled
implants
To
this
aim,
we
first
entrap
extracts
(CFEs)
from
Escherichia
coli
containing
transcription–translation
machinery,
together
with
plasmid
DNA
encoding
super
folded
green
fluorescence
(sGFP)
a
model
protein,
into
using
various
preparation
methods.
Agarose
result
most
suitable
confine
system,
demonstrating
efficient
sGFP
production
diffusion
core
surface
hydrogel.
Freeze-drying
(FD)
agarose
still
allows
for
sGFP,
yielding
more
attractive
biomaterial
its
reconstitution
FD-agarose
biocompatible
vitro,
allowing
colonization
cell
microenvironments
along
proliferation.
Implantation
assays
preclinical
mouse
proved
feasibility
approach
an
context
indicated
physical
properties
influence
immune
responses.
work
introduces
promising
avenue
fabrication,
enabling
targeted
delivery
opening
new
paths
advanced
approaches
based
biomaterials.
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(27)
Published: April 24, 2024
Abstract
Artificial
vesicles
are
recognized
as
powerful
platforms
for
a
large
body
of
research
across
the
disciplines
chemistry,
physics
and
biology.
Despite
great
progress,
control
size
distribution
to
make
uniform
remains
fundamentally
difficult
due
highly
uncontrollable
growth
kinetics,
especially
micron‐sized
vesicles.
Here
we
report
template‐free
living
self‐assembly
method
prepare
monodisperse
around
1
μm
from
an
alternating
copolymer.
The
polymer
forms
nanodisks
(
ca
.
9
nm)
in
N,N
‐dimethylformamide
(DMF),
acting
seeds
subsequent
growth.
By
adding
water,
gradually
grow
into
larger
circular
bilayer
nanosheets,
which
bend
crowns
continue
first‐order
kinetics
well
small
polydispersity
index
(<0.1)
suggests
characteristics.
This
work
paves
new
way
both
Small Methods,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 11, 2024
Abstract
Microfluidic
flow
reactors
permit
the
implementation
of
sensitive
biocatalysts
in
polymeric
environments
(e.g.,
hydrogel
dots),
mimicking
nature
through
use
diverse
microstructures
within
defined
confinements.
However,
establishing
complex
hybrid
structures
to
mimic
biological
processes
and
functions
under
continuous
with
optimal
utilization
all
components
involved
reaction
process
represents
a
significant
scientific
challenge.
To
achieve
spatial,
chemical,
temporal
control
for
any
microfluidic
application,
compartmentalization
is
required,
as
well
unification
different
compartments
chamber
design.
This
study
presents
self‐regulating
system
fabricated
by
sequential
photostructuring
an
intermediate
chemical
step
realize
pH‐sensitive
fabrication
double
reactor
controlled
enzymatic
cascade
(ECR).
The
key
point
adaptation
retention
function
pH‐responsive
horseradish
peroxidase‐loaded
polymersomes
chip
flow.
ECR
successfully
triggered
interplay
between
glucose
oxidase‐converted
glucose,
membrane
state
polymersomes,
other
parameters
rate
fluid
composition).
establishes
promising
noninvasive
regulatory
platform
extended
spatio–chemical
current
future
systems.
ACS Macro Letters,
Journal Year:
2024,
Volume and Issue:
unknown, P. 43 - 50
Published: Dec. 19, 2024
This
Letter
presents
complex
coacervation
between
the
biopolymer
diethylaminoethyl
dextran
hydrochloride
(DEAE-Dex)
and
carbon
dots.
The
formation
of
these
coacervates
was
dependent
on
both
DEAE-Dex
concentration
solution
ionic
strength.
Fluorescence
spectroscopy
revealed
that
blue
fluorescence
dots
unaffected
by
coacervation.
Additionally,
microrheological
studies
were
conducted
to
determine
viscosity
coacervates.
These
coacervates,
formed
through
interaction
nanoparticles
polyelectrolytes,
hold
a
promising
role
for
future
applications
where
combination
optical
properties
from
encapsulation
via
can
be
leveraged.
