An All‐in‐One “4A Hydrogel”: through First‐Aid Hemostatic, Antibacterial, Antioxidant, and Angiogenic to Promoting Infected Wound Healing

Small, Journal Year: 2023, Volume and Issue: 19(27)

Published: March 28, 2023

Abstract Currently used wound dressings are ineffective. Hence, there is a need to develop introduce high‐performance medicament with multiple functions including rapid hemostasis and excellent antibacterial activity meet the growing worldwide demand for healing products. Here, inspired by strong adhesion of mussels enzyme‐mimicking nanometallic biomaterials, authors developed an injectable hydrogel overcome limitations current dressings. The synthesized via esterification reaction between poly(vinyl alcohol) (PVA) 3,4‐dihydroxyphenylalanine (DOPA), followed catechol‐metal coordination Cu 2+ catechol groups DOPA form PVA‐DOPA‐Cu (PDPC) hydrogel. PDPC possesses tissue adhesive, antioxidative, photothermal, antibacterial, hemostatic properties. rapidly efficiently stopped bleeding under different traumatic conditions, otherwise‐lethal liver injury, high‐pressure carotid artery rupture, even fatal cardiac penetration injuries in animal models. Furthermore, it demonstrated that affected repair regeneration accelerating re‐epithelialization, promoting collagen deposition, regulating inflammation, contributing vascularization. results show promising candidate hemorrhage control efficient clinical applications.

Language: Английский

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A self-stabilized and water-responsive deliverable coenzyme-based polymer binary elastomer adhesive patch for treating oral ulcer

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 24, 2023

Oral ulcer can be treated with diverse biomaterials loading drugs or cytokines. However, most patients do not benefit from these materials because of poor adhesion, short-time retention in oral cavity and low drug therapeutic efficacy. Here we report a self-stabilized water-responsive deliverable coenzyme salt polymer poly(sodium α-lipoate) (PolyLA-Na)/coenzyme poly(α-lipoic acid) (PolyLA) binary synergistic elastomer adhesive patch, where hydrogen bonding cross-links between PolyLA PolyLA-Na prevents depolymerization slow down the dissociation PolyLA-Na, thus allowing sustainable delivery bioactive LA-based small molecules durable adhesion to mucosal wound due action PolyLA. In model mice mini-pig ulcer, patch accelerates healing by regulating damaged tissue inflammatory environment, maintaining stability microbiota, promoting faster re-epithelialization angiogenesis. This provided strategy treat ulcer.

Language: Английский

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Highly absorbent bio-sponge based on carboxymethyl chitosan/poly-γ-glutamic acid/platelet-rich plasma for hemostasis and wound healing

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 247, P. 125754 - 125754

Published: July 8, 2023

Language: Английский

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Engineering homologous platelet-rich plasma, platelet-rich plasma-derived exosomes, and mesenchymal stem cell-derived exosomes-based dual-crosslinked hydrogels as bioactive diabetic wound dressings

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 74 - 94

Published: May 17, 2023

The management of diabetic wounds remains a critical therapeutic challenge. Platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived (MSC-Exos) have demonstrated potential in wound treatment. Unfortunately, their poor mechanical properties, the short half-lives growth factors (GFs), burst release GFs limited clinical applications. Furthermore, proteases degrade GFs, which hampers repair. Silk fibroin is an enzyme-immobilization biomaterial that could protect from proteases. Herein, we developed novel dual-crosslinked hydrogels based on silk protein (SP) (sericin fibroin), including SP@PRP, SP@MSC-Exos, SP@PRP-Exos, to promote healing synergistically. SP@PRP was prepared PRP SP using calcium gluconate/thrombin as agonist, while SP@PRP-Exos SP@MSC-Exos were derived with genipin crosslinker. provided improved properties enabled sustained exosomes, thereby overcoming limitations healing. displayed shear-induced thinning, self-healing, eradication microbial biofilms bone-mimicking environment. In vivo, contributed faster than by upregulating expression, down-regulating matrix metalloproteinase-9 promoting anti-NETotic effect, angiogenesis, re-epithelialization. Hence, these be translated into new generation dressings.

Language: Английский

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Flexible Modulation of Cellular Activities with Cationic Photosensitizers: Insights of Alkyl Chain Length on Reactive Oxygen Species Antimicrobial Mechanisms

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(35)

Published: May 26, 2023

Cationic photosensitizers have good binding ability with negatively charged bacteria and fungi, exhibiting broad applications potential in antimicrobial photodynamic therapy (aPDT). However, cationic often display unsatisfactory transkingdom selectivity between mammalian cells pathogens, especially for eukaryotic fungi. It is unclear which biomolecular sites are more efficient damage, owing to the lack of systematic research same photosensitizer system. Herein, a series aggregation-induced emission (AIE) derivatives (CABs) (using berberine (BBR) as core) different length alkyl chains successfully designed synthesized flexible modulation cellular activities. The BBR core can efficiently produce reactive oxygen species (ROS) achieve high-performance aPDT . Through precise regulation chain length, bindings, localizations, killing effects CABs achieved investigated systematically among bacteria, cells. found that intracellular active substances, not membranes, damage aPDT. Moderate enable effectively kill Gram-negative fungi light, while still maintaining excellent cell blood compatibility. This study expected provide theoretical strategic guidance construction selectivity.

Language: Английский

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