Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Organoids-on-a-chip exhibit significant potential for advancing disease modeling, drug screening, and precision medicine, largely due to their capacity facilitate interactions among organoids.
Language: Английский
Interdisciplinary materials, Journal Year: 2023, Volume and Issue: 2(5), P. 714 - 734
Published: Sept. 1, 2023
Abstract The three‐dimensional (3D) multicellular platforms prepared by cells or biomaterials have been widely applied in biomedical fields for the regeneration of complex tissues, exploration cell crosstalk, and establishment tissue physiological pathological models. Compared with traditional 2D culture methods, 3D are easier to adjust components structures extracellular matrix (ECM) because synthesis ECM use biomaterials. Moreover, also can customize distribution precisely design micro macro systems. Based on these typical advantages their increasingly important position field, this review summarizes present platforms. Herein, current divided into two major types: scaffold‐free scaffold‐based specific characteristics applications different types thoroughly introduced help readers understand how models affect regulate behaviors inspire researchers select suitable according application scenarios.
Language: Английский
Citations
14
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4020 - 4020
Published: April 4, 2024
Biomaterials play an important role in the development of advancing three dimensional (3D) vitro skin models, providing valuable insights for drug testing and tissue-specific modeling. Commercial materials, such as collagen, fibrin or alginate, have been widely used However, they do not adequately represent molecular complexity components. On this regard, novel biomaterials that tissues is becoming more design advanced models. In study, we obtained aged human decellularized dermal extracellular matrix (dECM) hydrogels extracted from cadaveric demonstrated their potential scaffold These dECM effectively reproduce complex fibrillar structure other common scaffolds, exhibiting similar mechanical properties, while preserving composition native dermis. It worth noting fibroblasts embedded within exhibit a behavior representative natural compared to commercial collagen hydrogels, where uncontrolled cell proliferation leads material shrinkage. The described hydrogel able be aging-on-a-chip model. results demonstrate preserve essential components dermis making them suitable option 3D aging models accurately cellular microenvironment, improving existing allowing reliable dermatopathological studies.
Language: Английский
Citations
5
Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 224 - 224
Published: Jan. 19, 2024
Biocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced vitro for human skin. Silicone has been shown to be noncytotoxic but capable eliciting an immune response. Our aim was (1) establish a equivalent (2) assess the proinflammatory properties silicone. We developed coculture keratinocytes and fibroblasts resulting with implant using samples from breast implant. Samples without silicone were studied histologically immunohistochemically comparison native samples. Cytotoxicity assessed via LDH-assay, cytokine response ELISA. Histologically, our had four-layered epidermal dermal component. The presence tight junctions demonstrated immunofluorescence. only difference implants thinning. Implanting did not cause more death, however, inflammatory triggered. able organotypical implant, which can utilised studies on biocompatibility materials. This first integration into confirmed previous findings being non-cell-toxic exerting effect.
Language: Английский
Citations
4
Biomaterials, Journal Year: 2025, Volume and Issue: 317, P. 123074 - 123074
Published: Jan. 7, 2025
Language: Английский
Citations
0
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Organoids-on-a-chip exhibit significant potential for advancing disease modeling, drug screening, and precision medicine, largely due to their capacity facilitate interactions among organoids.
Language: Английский
Citations
0