Nano Today, Journal Year: 2024, Volume and Issue: 61, P. 102563 - 102563
Published: Dec. 1, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(22)
Published: April 13, 2024
Abstract The precise regulation of cellular behaviors within a confined, crowded intracellular environment is highly amenable in diagnostics and therapeutics. While synthetic circuitry system through concatenated chemical reaction network has rarely been reported to mimic dynamic self‐assembly system. Herein, catalytic self‐defined circuit (CSC) for the hierarchically assembly DNA domino nanostructures engineered. By incorporating pre‐sealed symmetrical fragments into preying hairpin reactants, CSC allows hierarchical via microRNA (miRNA)‐powered self‐sorting hybridization reaction. With minimal strand complexity, this self‐sustainable streamlined component achieved localization‐intensified cascaded signal amplification. Profiting from self‐adaptively reaction, reliable robust method discriminating carcinoma tissues corresponding para‐carcinoma tissues. CSC‐sustained strategy provides comprehensive smart toolbox organizing various nanostructures, which may facilitate more insights clinical diagnosis therapeutic assessment.
Language: Английский
Citations
13
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(23), P. 9666 - 9675
Published: May 30, 2024
Epigenetic modification plays an indispensable role in regulating routine molecular signaling pathways, yet it is rarely used to modulate self-assembly networks. Herein, we constructed a bioorthogonal demethylase-stimulated DNA circuitry (DSC) system for high-fidelity imaging of microRNA (miRNA) live cells and mice by eliminating undesired off-site signal leakage. The simple robust DSC composed primary cell-specific regulation (CR) module ultimate signal-transducing amplifier (SA) module. After the modularly designed was delivered into target cells, DNAzyme CR site-specifically activated endogenous demethylase produce fuel strands subsequent miRNA-targeting SA Through on-site multiply guaranteed recognitions, lucid efficient realized reliably amplified vivo miRNA sensing enabled in-depth exploration demethylase-involved pathway with cells. Our bioorthogonally on-site-activated represents universal versatile biomolecular platform via various regulations shows more prospects different personalized theragnostics.
Language: Английский
Citations
10
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
An entropy-driven catalysis (EDC) strategy is appealing for amplified bioimaging of microRNAs in living cells; yet, complex operation procedures, lacking cell selectivity, and insufficient accuracy hamper its further applications. Here, we introduce an ingenious all-in-one DNA nanomachine (termed as AIO-EDN), which can be triggered by endogenous apurinic/apyrimidinic endonuclease 1 (APE1) to achieve tumor cell-selective dual-mode imaging microRNA. Compared with the traditional EDC strategy, integrated design AIO-EDN achieves autocatalytic signal amplification without extra fuel strands. Moreover, leverages APE1 overexpressed cancer cells activate reaction, which, however, exerts no target sensing activity normal cells. Combining fluorescence- surface-enhanced Raman scattering (FL/SERS) techniques, this exhibits significantly improved selectivity microRNA This study provides a new paradigm develop EDC-based platform shows great potential in-depth diagnosis high precision.
Language: Английский
Citations
1
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(31), P. 12854 - 12861
Published: July 23, 2024
Sensitive and reliable microRNA imaging in living cells has significant implications for clinical diagnosis monitoring. Catalytic DNA circuits have emerged as potent tools tracking these intracellular biomarkers probing the corresponding biochemical processes. However, their utility is hindered by low resistance to external interference, leading undesired off-site activation consequent signal leakage. Therefore, achieving endogenous control of circuit's preferable target analysis cells. In this study, we attempted address challenge engineering a simple yet effective glutathione (GSH)-regulated hybridization chain reaction (HCR) circuit acquiring high-contrast miRNA imaging. Initially, HCR hairpin reactants were blocked engineered disulfide-integrated duplex, thus effectively passivating sensing function. And precaged was liberated cell-specific GSH molecule, initiating system selectively amplified detection microRNA-21 (miR-21). This approach prevented unwanted leakage before exposure into cells, ensuring robust miR-21 with high accuracy reliability specific tumor Moreover, endogenously responsive established link between small regulatory factors miRNA, thereby enhancing gain. summary, activatable represents versatile toolbox bioanalysis exploration potential signaling pathways
Language: Английский
Citations
5
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159310 - 159310
Published: Jan. 5, 2025
Language: Английский
Citations
0
Small, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Abstract DNA circuits show great potential in monitoring intracellular biomarkers based on their high programmability, predictability, and unique signal amplification capabilities, yet face challenges from uncontrollable leakage caused by the complex environment. Herein, a demethylase‐activated DNA‐assembly (DAD) circuit is designed for reliable robust imaging of cellular microRNA, incorporating sequential activation hybridization chain reaction (HCR) amplifier system. The DAD consists DNAzyme module microRNA‐recognizing HCR signal‐amplifying module. m 6 A‐modified sequence module, initially possessing temporally caged substrate‐cleavage activity, integrated into probe effectively blocking its miRNA‐sensing capacity. In presence ALKBH5 demethylase, methyl‐modifying unit removed, thus restoring catalytic substrate‐cleaving activity. This process exposed previously toehold region probe, thereby activating sensing miRNA. By leveraging activation, this can substantially enhance signal‐to‐background ratio, enabling highly sensitive miRNA detection efficient differentiation cancerous normal cells. Furthermore, established relationship between demethylase enzyme miRNA, paving way investigating more complicate biological processes intricate signaling pathways within
Language: Английский
Citations
0
ChemBioChem, Journal Year: 2024, Volume and Issue: 25(15)
Published: May 27, 2024
Abstract Nucleic acids exhibit exceptional functionalities for both molecular recognition and catalysis, along with the capability of predictable assembly through strand displacement reactions. The inherent programmability addressability DNA probes enable their precise, on‐demand accurate execution hybridization, significantly enhancing target detection capabilities. Decades research in nanotechnology have led to advances structural design functional probes, resulting increasingly sensitive robust sensors. Moreover, increasing attention has been devoted accuracy sensitivity DNA‐based biosensors by integrating multiple sensing procedures. In this review, we summarize various strategies aimed at These involve guarantee procedures, utilizing dual signal output mechanisms, implementing sequential regulation methods. Our goal is provide new insights into development more sensors, ultimately facilitating widespread application clinical diagnostics assessment.
Language: Английский
Citations
2
ChemBioChem, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 14, 2024
The ability to precisely control the function of nucleic acids plays an important role in biosensing and biomedicine. In recent years, novel strategies employing biological, physical, chemical triggers have been developed modulate spatiotemporally. These approaches commonly involve incorporation stimuli-responsive groups onto block their functions until triggers-induced decaging restore activity. inventive deepen our comprehension acid molecules' dynamic behavior provide new techniques for precise disease diagnosis treatment. Focusing on spatiotemporal regulation molecules through caging-decaging strategy, we here present overview innovative triggered mechanisms accentuate implications across fields biology, biomedicine, biosensing.
Language: Английский
Citations
2
Small, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 16, 2024
Abstract Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion‐dominated slow reaction kinetics uncontrollable off‐target activation. Herein, self‐localized cascade (SLC) circuit reported robust efficient microRNA (miRNA) analysis living The SLC consists cell‐specific localization module analyte‐specific signal amplification module. By integrating probes these two modules, complexity system reduced to realize responsive co‐localization circuitry simultaneous amplification. Taking advantage specifically activated, self‐localized, design, successfully achieves miRNA‐21 (miR‐21) imaging accurate cells differentiation. Moreover, reverse mechanism explored between messenger RNA (mRNA) miRNA through engineered further elucidates underlying signaling pathways them. Therefore, provides powerful tool sensitive detection intracellular biomolecules study corresponding cell regulatory mechanisms.
Language: Английский
Citations
2
Nano Today, Journal Year: 2024, Volume and Issue: 61, P. 102563 - 102563
Published: Dec. 1, 2024
Language: Английский
Citations
1