Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1869(7), P. 166806 - 166806
Published: July 11, 2023
Language: Английский
Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522
Published: Dec. 21, 2022
Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.
Language: Английский
Citations
259
Bioactive Materials, Journal Year: 2023, Volume and Issue: 30, P. 169 - 183
Published: Aug. 4, 2023
Osteoarthritis (OA) is the most common disabling joint disease with no effective modifying drugs. Extracellular vesicles released by several types of mesenchymal stem cells could promote cartilage repair and ameliorate OA pathology in animal models, representing a novel therapeutic strategy. In this study, we demonstrated that extracellular derived from human umbilical cord (hUC-EVs) maintain chondrocyte homeostasis alleviate OA, further revealed molecular mechanism effect. miR-223, which directly bind 3'UTR NLRP3 mRNA, was found to be key miRNA for hUC-EVs exert beneficial effects on inflammation inhibiting protecting. For enhancing effect mitigating osteoarthritis, exogenous miR-223 loaded into electroporation, collagen II-targeting peptide (WYRGRL) modified onto surface genetic engineering achieve more targeted efficient RNA delivery cartilage. The dual-engineered EVs showed maximal inflammasome activation pyroptosis, offered excellent results treatment OA. This study provides theoretical basis promising strategy application engineered treatment.
Language: Английский
Citations
47
Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2099 - 2126
Published: Jan. 1, 2024
Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.
Language: Английский
Citations
20
Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 3, 2025
Language: Английский
Citations
6
Biomaterials Advances, Journal Year: 2024, Volume and Issue: 158, P. 213770 - 213770
Published: Jan. 13, 2024
Recent research indicates that combining 3D bioprinting and small extracellular vesicles (sEVs) offers a promising 'cell-free' regenerative medicine approach for various tissue engineering applications. Nonetheless, the majority of existing has focused on sEVs sourced from cell lines. There remains notable gap in regarding derived primary human periodontal cells their potential impact ligamentous osteogenic differentiation. Here, we investigated effect bioprinted constructs differentiation buccal fat pad-derived mesenchymal stromal (hBFP-MSCs). Periodontal cell-derived were enriched by size exclusion chromatography (SEC) with particle-shaped morphology, characterized being smaller than 200 nm CD9/CD63/CD81 positive, ligament (hPDLCs) gingival fibroblasts (hGFs). The then 10 % gelatin methacryloyl (GelMA) via microextrusion bioprinting. Release was determined DiO-labelling confocal imaging, CD9 ELISA. Attachment ligament/osteogenic/cementogenic hBFP-MSCs assessed GelMA, without (GelMA/hPDLCs-sEVs GelMA/hGFs-sEVs), scaffolds. seeded spread well significantly enhanced focal adhesion, mechanotransduction associated gene expression, osteogenesis/cementogenesis markers GelMA/hPDLCs-sEVs, compared to GelMA/hGFs-sEVs GelMA groups. A 2-week showed ALP staining, calcium formation toluidine blue stained GelMA/hPDLCs-sEVs other two proof-of-concept data this study supports notion scaffolds promote attachment, as ligamentous, cementogenic differentiation, vitro.
Language: Английский
Citations
11
Deleted Journal, Journal Year: 2024, Volume and Issue: 2(3)
Published: May 29, 2024
Abstract The skin interfaces with the external environment, acting as both a physical barrier and an immunologic defense. dynamic interactions between various cell types are essential for homeostasis function. Emerging research has unveiled significant role of extracellular vesicles (EVs) key mediators communication in regulating many aspects physiological pathological processes. In this review, we provide overview recent advances understanding roles EVs health, aging, diseases, discuss natural engineered disease treatment along potential challenges. burgeoning area may expand new therapeutic approaches disorders open avenues personalized skincare treatments.
Language: Английский
Citations
10
Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 260 - 274
Published: June 1, 2024
Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies CRISPR gene editing, microfluidic manufacturing, biomimetic systems are highlighted their potential enhance MSC-EV targeting, optimize enrichment, ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment tumorigenicity, immunogenicity, biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science patient advocacy hold the expediting clinical translation. By overcoming these challenges, could catalyze new era of off-the-shelf therapies, restoring hope afflicted by non-healing
Language: Английский
Citations
10
Burns & Trauma, Journal Year: 2024, Volume and Issue: 12
Published: Jan. 1, 2024
Abstract Extracellular vesicles (EVs) are heterogeneous membrane-like secreted by living cells that involved in many physiological and pathological processes act as intermediaries of intercellular communication molecular transfer. Recent studies have shown EVs from specific sources regulate tissue repair regeneration delivering proteins, lipids, nucleic acids to target signaling molecules. Nanotechnology breakthroughs facilitated the development exploration engineered for repair. Enhancements through gene editing, surface modification, content modification further improved their therapeutic efficacy. This review summarizes potential regeneration, mechanisms action, research progress regenerative medicine. highlights design logic typical examples explores prospects The aim this is provide new insights into applications, thereby expanding use
Language: Английский
Citations
10
Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 149 - 170
Published: Feb. 18, 2025
Language: Английский
Citations
1
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 25, 2025
Cavernous nerve injury-induced erectile dysfunction (CNI-ED) is a common complication after radical prostatectomy. Conventional treatment approaches have had little success in treating the severe cavernous fibrosis which consequence of CNI-ED. Pre-treatment adipose-derived stem cells with melatonin allows for extraction active exosomes (MT-hASC-EVs) from conditioned medium. The therapeutic effects MT-hASC-EVs were assessed rat model CNI-ED, and anti-fibrotic properties evaluated. MicroRNA sequencing was used to identify specific microRNAs highly expressed MT-hASC-EVs, differential screened regulatory pathways through target gene enrichment analysis. Finally, conclusions bioinformatics analysis validated vitro experiments. Intracavernous injection significantly restored function reduced extent corpus cavernosum CNI-ED model. promoted proliferation anti-apoptotic smooth muscle (CCSMCs) vitro. Mechanistically, inhibit by delivering miR-145-5p, targets TGF-β2/Smad3 axis. MT-hASCs-EVs can improve targeting miR-145-5p/TGF-β/Smad
Language: Английский
Citations
1