Biology, Journal Year: 2024, Volume and Issue: 14(1), P. 1 - 1
Published: Dec. 24, 2024
Regenerative medicine is the new frontier of [...].
Language: Английский
Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
2
Engineering in Life Sciences, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 1, 2025
ABSTRACT The development of an effective and rapid method for healing the skin is crucial importance. In this study, we prepared a porous scaffold made polycaprolactone (PCL) carbon quantum dots (CQDs), Fe, Chitosan (Cs) as core to cover skin. Then evaluated antibacterial, biocompatibility, wound properties well expression genes in healing. PCL/Cs/CQD‐Fe was synthesized via electrospinning morphology, functional groups, structure through Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), x‐ray diffraction (XRD). viability L929 fibroblast stem cells obtained. antibacterial effect, efficiency were investigated minimum inhibitory concentration (MIC), (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), tissue analysis. relative platelet‐derived growth factor (PDGF), transforming beta (TGF‐β), matrix metalloproteinase‐1 (MMP1) assessed RT‐PCR. results SEM showed successful integration PCL with CQD‐Fe Cs. mean size nanocomposite range 0.135–32.6 nm. FTIR formation link between CQD nanoparticles Fe. vibrating‐sample magnetometer (VSM) proved super para magnetism magnetic (0.38 emu/g). MIC Cs/CQD‐Fe against Staphylococcus aureus Escherichia coli bacteria 0.08 0.04 µg/mL, respectively. TGF‐β PDGF group 0.05 0.015 on day 5 0.18 0.34 15 significantly increased after days, whereas MMP1 0.63 0.12 decreased days. According histological analysis, thickest layer Day pertained group. Our findings indicated that can improve regeneration due its non‐toxicity. This biocompatible be used covering
Language: Английский
Citations
1
Regenerative Therapy, Journal Year: 2025, Volume and Issue: 28, P. 558 - 572
Published: Feb. 13, 2025
Language: Английский
Citations
0
Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)
Published: March 1, 2025
ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.
Language: Английский
Citations
0
Regenerative Therapy, Journal Year: 2025, Volume and Issue: 29, P. 303 - 318
Published: April 5, 2025
Language: Английский
Citations
0
Journal of Molecular Histology, Journal Year: 2025, Volume and Issue: 56(2)
Published: April 1, 2025
Language: Английский
Citations
0
MedComm, Journal Year: 2025, Volume and Issue: 6(5)
Published: April 24, 2025
ABSTRACT From the pioneering days of cell therapy to achievement bioprinting organs, tissue engineering, and regenerative medicine have seen tremendous technological advancements, offering solutions for restoring damaged tissues organs. However, only a few products technologies received United States Food Drug Administration approval. This review highlights significant progress in therapy, extracellular vesicle‐based engineering. Hematopoietic stem transplantation is powerful tool treating many diseases, especially hematological malignancies. Mesenchymal cells been extensively studied. The discovery induced pluripotent has revolutionized disease modeling applications, paving way personalized medicine. Gene represents an innovative approach treatment genetic disorders. Additionally, therapies emerged as rising stars, promising diagnostics, cell‐free therapeutics, drug delivery, targeted therapy. Advances engineering enable complex constructs, further transforming field. Despite these technical, ethical, regulatory challenges remain. addresses current bottlenecks, emphasizing novel interdisciplinary research overcome hurdles. Standardizing practices conducting clinical trials will balance innovation regulation, improving patient outcomes quality life.
Language: Английский
Citations
0
Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 6, 2024
Language: Английский
Citations
0
Cell Proliferation, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 20, 2024
Osteoarthritis (OA), a joint disease, burdens global healthcare due to aging and obesity. Recent studies show that extracellular vesicles (EVs) from bone marrow-derived mesenchymal stem cells (BMSCs) contribute homeostasis OA management. However, the impact of donor age on BMSC-derived EV efficacy remains underexplored. In this study, we investigated young BMSCs (2-month-old) in mitigating OA, contrasting them with EVs aged (27-month-old). The study used destabilisation medial meniscus (DMM) surgery mouse knee joints induce accelerated OA. Cartilage degeneration markers senescence markers' expression levels were response treatment. therapeutic chondrocytes under inflammatory responses was also evaluated. Despite having similar morphologies, markedly decreased improved chondroprotection by activating PTEN pathway while simultaneously suppressing upregulation PI3K/AKT pathways, proving be more effective than those older vitro. Furthermore, intraperitoneal injections donors significantly mitigated progression preserving cartilage reducing synovitis surgical model using DMM mice. These findings highlight as critical determinant potential for clinical use
Language: Английский
Citations
0
Biology, Journal Year: 2024, Volume and Issue: 14(1), P. 1 - 1
Published: Dec. 24, 2024
Regenerative medicine is the new frontier of [...].
Language: Английский
Citations
0