Extracellular
vesicles
(EVs)
hold
enormous
potential
in
the
diagnosis
and
treatment
of
diseases,
benefit
from
their
roles
intercellular
signaling,
as
well
plentiful
presence
stability
body
fluids.
However,
conventional
EV
isolation
methods
are
labor-intensive,
harvest
EVs
with
low
purity
compromised
recovery.
In
addition,
drawbacks,
such
limited
sensitivity
specificity
traditional
analysis
hinder
application
clinical
use.
Therefore,
it
is
urgent
to
develop
effective
standardized
for
isolating
detecting
EVs.
Microfluidics
technology
a
powerful
rapidly
developing
that
has
been
introduced
solution
above
bottlenecks.
It
holds
advantages
high
integration,
short
time,
consumption
samples
reagents.
this
review,
we
summarize
techniques
alongside
microfluidic-based
methodologies
detection
We
emphasize
separation
microfluidic
enhancing
capture
efficiency
precise
targeting,
while
summarizing
its
analytical
role
targeted
discussing
significance
tumor
microenvironment,
providing
direction
achieving
automated
high-throughput
samples.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2144 - 2144
Published: Feb. 10, 2024
The
members
of
the
Flaviviridae
family
are
becoming
an
emerging
threat
for
public
health,
causing
increasing
number
infections
each
year
and
requiring
effective
treatment.
consequences
these
can
be
severe
include
liver
inflammation
with
subsequent
carcinogenesis,
endothelial
damage
hemorrhage,
neuroinflammation,
and,
in
some
cases,
death.
mechanisms
pathogenesis
being
actively
investigated,
but
there
still
many
gaps
their
understanding.
Extracellular
vesicles
may
play
important
roles
mechanisms,
therefore,
this
topic
deserves
detailed
research.
Recent
data
have
revealed
involvement
extracellular
steps
such
as
transmission,
immune
evasion,
inflammation,
which
is
critical
disease
establishment.
This
review
covers
recent
papers
on
includes
examples
clinical
applications
accumulated
data.
Extracellular
vesicles
(EVs)
hold
enormous
potential
in
the
diagnosis
and
treatment
of
diseases,
benefit
from
their
roles
intercellular
signaling,
as
well
plentiful
presence
stability
body
fluids.
However,
conventional
EV
isolation
methods
are
labor-intensive,
harvest
EVs
with
low
purity
compromised
recovery.
In
addition,
drawbacks,
such
limited
sensitivity
specificity
traditional
analysis
hinder
application
clinical
use.
Therefore,
it
is
urgent
to
develop
effective
standardized
for
isolating
detecting
EVs.
Microfluidics
technology
a
powerful
rapidly
developing
that
has
been
introduced
solution
above
bottlenecks.
It
holds
advantages
high
integration,
short
time,
consumption
samples
reagents.
this
review,
we
summarize
techniques
alongside
microfluidic-based
methodologies
detection
We
emphasize
separation
microfluidic
enhancing
capture
efficiency
precise
targeting,
while
summarizing
its
analytical
role
targeted
discussing
significance
tumor
microenvironment,
providing
direction
achieving
automated
high-throughput
samples.
