DNA Hydrogels for Cancer Diagnosis and Therapy DOI Open Access

Li Peng,

Suang Gu,

Min Hou

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Abstract Nucleic acids, because of their precise pairing and simple composition, have emerged as excellent materials for the formation gels. The application DNA hydrogels in diagnosis therapy cancer has expanded significantly through research on properties functions nucleic acids. Functional acids (FNAs) such aptamers, Small interfering RNA (siRNA), DNAzymes been incorporated into to enhance diagnostic therapeutic capabilities. This review discusses various methods forming hydrogels, with a focus pure hydrogels. We then explore innovative applications therapy. become essential biomedical materials, this provides an overview current findings status cancer, while also exploring future directions.

Language: Английский

Functionalized hydrogels as smart gene delivery systems to treat musculoskeletal disorders DOI Creative Commons

Mohammadsaeid Enayati,

Wei Liu, Henning Madry

et al.

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 331, P. 103232 - 103232

Published: June 14, 2024

Despite critical advances in regenerative medicine, the generation of definitive, reliable treatments for musculoskeletal diseases remains challenging. Gene therapy based on delivery therapeutic genetic sequences has strong value to offer effective, durable options decisively manage such disorders. Furthermore, scaffold-mediated gene provides powerful alternatives overcome hurdles associated with classical therapy, allowing spatiotemporal candidate genes sites injury. Among many scaffolds research, hydrogels raised increasing attention addition other potent systems (solid, hybrid scaffolds) due their versatility and competence as drug cell carriers tissue engineering wound dressing. Attractive functionalities include injectability, stimuli-responsiveness, self-healing, nanocomposition that may further allow upgrade them "intelligently" efficient mechanically platforms, rather than just inert vehicles. Such functionalized also be tuned successfully transfer a minimally invasive manner order protect cargos long-term effects. In light features, this review focuses demonstrates treatment disorders using procedures, from principles hydrogel functionalization methods applications hydrogel-mediated disorders, while remaining challenges are being discussed perspective translation patients. offering Scaffold-mediated therapy. attention. Functionalities including tune This introduces disorder

Language: Английский

Citations

4
Exploring triplex DNA nanostructures: A comprehensive review from fundamentals to applications DOI
Shi‐Yang Tang, Baoshan He,

Lingling Xie

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159425 - 159425

Published: Jan. 1, 2025

Language: Английский

Citations

0
DNA Nanostructures‐Based In Situ Cancer Vaccines: Mechanisms and Applications DOI Open Access
Bingyu Lin, Yanfei Liu, Qi‐Wen Chen

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract Current tumor vaccines suffer from inadequate immune responsive due to the insufficient release of antigens, low infiltration, and immunosuppressive microenvironment. DNA nanostructures with their ability precisely engineer, controlled release, biocompatibility, capability augment immunogenicity microenvironment, have gained significant attention for potential revolutionize vaccine designing. This review summarizes various applications in construction situ cancer vaccines, which can generate tumor‐associated antigens directly damaged tumors immune‐stimulation. The mechanisms components are listed, specific strategies constructing using explored underlying action elucidated. immunogenic cell death (ICD) induced by chemotherapeutic agents, photothermal therapy (PTT), photodynamic (PDT), radiation (RT) related building systematically summarized. different immunotherapy elaborated, exerts precise, long‐lasting, robust responses. current challenges future prospectives proposed. provides a holistic understanding evolving role development.

Language: Английский

Citations

0
DNA Hydrogels in Tissue Engineering: From Molecular Design to Next‐Generation Biomedical Applications DOI
Ziyu Zhu, Yu Yang, Yun Jiang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract DNA hydrogels have emerged as promising materials in tissue engineering due to their biocompatibility, programmability, and responsiveness stimuli. Synthesized through physical chemical crosslinking, these can be categorized into functionalized types, such those based on aptamers, stimuli‐responsive types that react pH, temperature, light. This review highlights applications engineering, including drug delivery, cell culture, biosensing, gene editing. encapsulate therapeutic agents, support growth, respond dynamically environmental changes, making them ideal for engineering. A comprehensive bibliometric analysis is included, identifying key research trends emerging areas of interest hydrogel design, synthesis, biomedical applications. The provides a deeper understanding the field's development future directions. Challenges mechanical strength, stability, biosafety persist, but integration AI design shows promise advancing functionality clinical

Language: Английский

Citations

0
Smartphone-Based Chemiluminescence Glucose Biosensor Employing a Peroxidase-Mimicking, Guanosine-Based Self-Assembled Hydrogel DOI Creative Commons
Donato Calabria, Andrea Pace, Elisa Lazzarini

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(6), P. 650 - 650

Published: June 14, 2023

Chemiluminescence is widely used for hydrogen peroxide detection, mainly exploiting the highly sensitive peroxidase-luminol-H2O2 system. Hydrogen plays an important role in several physiological and pathological processes produced by oxidases, thus providing a straightforward way to quantify these enzymes their substrates. Recently, biomolecular self-assembled materials obtained guanosine its derivatives displaying peroxidase enzyme-like catalytic activity have received great interest biosensing. These soft are biocompatible can incorporate foreign substances while preserving benign environment biosensing events. In this work, guanosine-derived hydrogel containing chemiluminescent reagent (luminol) cofactor (hemin) was as H2O2-responsive material peroxidase-like activity. Once loaded with glucose oxidase, provided increased enzyme stability even alkaline oxidizing conditions. By 3D printing technology, smartphone-based portable chemiluminescence biosensor developed. The allowed accurate measurement of serum, including both hypo- hyperglycemic samples, limit detection 120 µmol L-1. This approach could be applied other enabling development bioassays biomarkers clinical at point care.

Language: Английский

Citations

10
Fabrication and Cell Culture Applications of Core‐Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations DOI

Yoshinobu Utagawa,

Kosuke Ino, Masahiro Takinoue

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(31)

Published: July 21, 2023

Core-shell hydrogel fibers are widely used in cell culture applications. A simple and rapid method is presented for fabricating core-shell fibers, consisting of straight or beaded core The prepared using interfacial polyelectrolyte complexation (IPC) with chitosan DNA. Briefly, two droplets DNA brought contact to form an IPC film, which dragged prepare fiber. incubation time concentration adjusted fibers. Ca

Language: Английский

Citations

10
Functionalities of pH-responsive DNA nanostructures in tumor-targeted strategies DOI
Lingrong Wen, Min Wang

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Nanostructures integrating pH-sensitive DNA motifs have emerged as versatile platforms for active tumor targeting, owing to their ability undergo conformation changes in response the common acidic environment of extracellular matrix and endocytosis pathway. This review summarizes latest advances design application various pH-responsive nanostructures tumor-targeted strategies, including recognition, cell imaging, dynamic nanocarrier construction, controlled drug release. A comprehensive framework pH-controlled multi-stage targeting is introduced, addressing divergences strategies intracellular environments. The unique attributes, practical performance challenges are also critically discussed provide guidance future development this field.

Language: Английский

Citations

3
Recent advances in shape memory scaffolds and regenerative outcomes DOI
Ferzane Valioğlu, Fereshteh Valipour,

Shadi Atazadeh

et al.

Biomedical Engineering Letters, Journal Year: 2024, Volume and Issue: 14(6), P. 1279 - 1301

Published: Aug. 21, 2024

Language: Английский

Citations

2
Construction of DNA aggregates in cell milieu for bio‐interference DOI Creative Commons
Yanfei Guo,

Jinqiao Liu,

Yan Huang

et al.

Aggregate, Journal Year: 2023, Volume and Issue: 4(6)

Published: July 22, 2023

Abstract Precise assembly of biomolecules into functional aggregate structures represents a key characteristic nature living cells, and is critical for the cellular processes. The construction artificial aggregates in cells by responding to intracellular specific stimuli has been applied elucidating molecular mechanisms naturally processes interfering with processes, which potential significant biomedicine. DNA (deoxyribonucleic acid) features sequence programmability, precise versatility, therefore regarded as candidate constructing versatile cells. In this review, we summarize our recent efforts employing situ construct via triggers, subsequent bio‐interference aggregates. Finally, discuss remaining challenges opportunities field, envision that rational design system would be promising solution precision personalized therapeutics.

Language: Английский

Citations

6
Effect of pH on the Poly(acrylic Acid)/Poly(vinyl Alcohol)/Lysozyme Complexes Formation DOI Creative Commons
Simona Morariu, Mihaela Avădanei, Loredana E. Niţă

et al.

Molecules, Journal Year: 2023, Volume and Issue: 29(1), P. 208 - 208

Published: Dec. 29, 2023

The interactions between poly(acrylic acid) (PAA), poly(vinyl alcohol) (PVA), and lysozyme (Lys) in an aqueous environment at pHs of 2, 4, 7.4 were discussed considering the experimental data obtained by turbidimetry, electrokinetic rheological measurements, FTIR analysis. It was found that increase PAA amount reduces coacervation zone shifting critical pHcr1to higher values while pHcr2 remains unchanged. extended from 3.1–4.2 to 2.9–4.7 increasing Lys concentration 0.2% 0.5%. zeta potential measurements showed PAA–PVA–Lys mixture water is most stable pH range 4.5–8. Zero shear viscosity exhibited deviations additivity both investigated pHs, a maximum value corresponding hydrodynamic volume revealed weight fractions 0.4 0.5 for 4 7.4, respectively. binding affinity PAA, established molecular dynamics simulation, slightly than PVA. more complex PAA–Lys formed very acidic environment; that, −7.1 kcal/mol determined.

Language: Английский

Citations

4