Optical Image Sensors for Smart Analytical Chemiluminescence Biosensors DOI Creative Commons
Reza Abbasi,

Xinyue Hu,

Alain Zhang

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(9), P. 912 - 912

Published: Sept. 12, 2024

Optical biosensors have emerged as a powerful tool in analytical biochemistry, offering high sensitivity and specificity the detection of various biomolecules. This article explores advancements integration optical with microfluidic technologies, creating lab-on-a-chip (LOC) platforms that enable rapid, efficient, miniaturized analysis at point need. These LOC leverage phenomena such chemiluminescence electrochemiluminescence to achieve real-time quantification analytes, making them ideal for applications medical diagnostics, environmental monitoring, food safety. Various detectors used detecting are reviewed, including single-point photomultiplier tubes (PMT) avalanche photodiodes (APD), pixelated charge-coupled devices (CCD) complementary metal-oxide-semiconductor (CMOS) sensors. A significant advancement discussed this review is image sensors, particularly CMOS sensors provide numerous advantages over traditional detectors, high-resolution imaging, spatially resolved measurements, ability simultaneously detect multiple analytes. Their compact size, low power consumption, cost-effectiveness further enhance their suitability portable point-of-care diagnostic devices. In future, machine learning algorithms these technologies promises data interpretation, driving development more sophisticated, accessible tools diverse applications.

Language: Английский

Electrochemical vs. Optical Biosensors for Point-of-Care Applications: A Critical Review DOI Creative Commons
Seyedeh Rojin Shariati Pour, Donato Calabria,

Afsaneh Emamiamin

et al.

Chemosensors, Journal Year: 2023, Volume and Issue: 11(10), P. 546 - 546

Published: Oct. 21, 2023

Analytical chemistry applied to medical and diagnostic analysis has recently focused on the development of cost-effective biosensors able monitor health status or assess level specific biomarkers that can be indicative several diseases. The improvement technologies relating possibility non-invasive sampling biological fluids, as well sensors for detection analytical signals computational capabilities systems routinely employed in everyday life (e.g., smartphones, computers, etc.), makes complete integration self-standing devices more accessible. This review aims discuss have been proposed last five years focusing two principal detecting approaches, optical electrochemical, which quantifying different kinds target analytes reaching limits below clinical sample levels required. These principles point-of-care (POC) extensively reported literature, even limited examples found market are based these strategies. work will show latest innovations considering electrochemical with most commonly platforms POC applications such paper-based wearable implantable devices.

Language: Английский

Citations

25
Trends in sensing of creatinine by electrochemical and optical biosensors DOI Creative Commons

Radhika Babasaheb Jadhav,

Tejaswini P. Patil,

Arpita Tiwari

et al.

Applied Surface Science Advances, Journal Year: 2024, Volume and Issue: 19, P. 100567 - 100567

Published: Jan. 10, 2024

Creatinine is the by-product of creatine phosphate within muscle, supplying energy to muscle tissues. It imperative clinically assess creatinine levels in both urine and blood as it serves an indicator renal, thyroid functionality. The point-of-care medical diagnostic research development most innovative form exploratory research. determination can be achieved through several traditional methods such colorimetric, spectrophotometric chromatographic techniques. Although these method offers high sensitivity selectivity, they are accompanied by drawback long analysis time, need for sample pre-treatment, expensive instruments skilled personnel. In contrast, sensors biosensors present a favourable solution limitations offer rapid, user-friendly, cost effective highly sensitive alternatives. This review article describe recent trends detection using electrochemical optical biosensors, advantages disadvantages biosensors. highlights wide range explore commercialization aspects with home monitoring system.

Language: Английский

Citations

11
Deep Learning-Enhanced Portable Chemiluminescence Biosensor: 3D-Printed, Smartphone-Integrated Platform for Glucose Detection DOI Creative Commons

Chirag M. Singhal,

V. Kaushik,

Amit K. Awasthi

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 119 - 119

Published: Jan. 27, 2025

A novel, portable chemiluminescence (CL) sensing platform powered by deep learning and smartphone integration has been developed for cost-effective selective glucose detection. This features low-cost, wax-printed micro-pads (WPµ-pads) on paper-based substrates used to construct a miniaturized CL sensor. 3D-printed black box serves as compact WPµ-pad chamber, replacing traditional bulky equipment, such charge coupled device (CCD) cameras optical sensors. Smartphone enables seamless user-friendly diagnostic experience, making this highly suitable point-of-care (PoC) applications. Deep models significantly enhance the platform’s performance, offering superior accuracy efficiency in image analysis. dataset of 600 experimental images was utilized, out which 80% were model training, with 20% reserved testing. Comparative analysis conducted using multiple models, including Random Forest, Support Vector Machine (SVM), InceptionV3, VGG16, ResNet-50, identify optimal architecture accurate The sensor demonstrates linear detection range 10–1000 µM, low limit 8.68 µM. Extensive evaluations confirmed its stability, repeatability, reliability under real-world conditions. learning-powered not only improves analyte detection, but also democratizes access advanced diagnostics through technology. work paves way next-generation biosensing, transformative potential healthcare other domains requiring rapid reliable

Language: Английский

Citations

1
Recent advances and trends in optical devices and sensors for hydrogen peroxide detection DOI Creative Commons

John J. Galligan,

Antje J. Baeumner, Axel Duerkop

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117948 - 117948

Published: Sept. 3, 2024

Hydrogen peroxide (H2O2) is a critically important, vital biomarker and hence highly relevant analyte in broad range of bioanalytical applications. The most recent trends furthering the ability its reliable, reproducible, sensitive quantification include development non-biological enzyme mimics, investigation smartphone cameras as transducers detectors, continued semi-reversible reversible detection strategies. While catalysts offer stability-related advantages over enzymes while providing equally good limits detection, critical questions regarding toxicity, persistence, (bio)accumulation, overall environmental footprint need to be answered. In case heavy metal-based strategies replacement by non-toxic, renewable alternatives should an obvious research need. Signal recording has seen dramatic change toward smartphones, with their ever-improving computing image-acquisition abilities. Yet, sheer number different camera phone models progress can difficult assess, reproducibility comparability results experimental set-ups are too often elusive. area sensors flow injection analysis (FIA) coupled chemiluminescence (CL) remains advanced system. fully sensors, points oxygen-based sensing reliable. Analyzing publications from 20198-20234, it not surprising that important analytical figures merit low (LODs), quantitation ranges, faster response regeneration times combined novel (reversible) probes continue remain central focus future developments.

Language: Английский

Citations

7
Easy-to-Use Chemiluminescent-Based Assay for a Rapid and Low-Cost Evaluation of the Antioxidant Activity of Cosmetic Products DOI Creative Commons
Seyedeh Rojin Shariati Pour, Donato Calabria, A. Nascetti

et al.

Chemosensors, Journal Year: 2024, Volume and Issue: 12(2), P. 25 - 25

Published: Feb. 9, 2024

New cosmetic formulations are continuously requested by the market and ingredients constantly evolving. Recently use of antioxidants has gained success and, in this context, analytical methods able to quickly easily assess antioxidant activity cosmetics would make it possible carry out analyses on new even within manufacturing process without need for specialized laboratories personnel, thus evaluating directly on-site effectiveness shelf life products. In work, a chemiluminescent inhibition assay was developed determining total The method based luminol/enhancers/hydrogen peroxide/horseradish peroxidase system, which generates light signals measurable through simple compact instrumentation. formation signal is inhibited presence substances while restored once all molecules have been oxidized. time appearance related activity. carried exploiting an integrated device comprising microwell plate coupled with array amorphous silicon hydrogenated photosensors enclosed mini-dark box. optimized terms concentrations volumes required reagents sample pre-treatment. A calibration curve generated taking as reference ascorbic acid obtaining detection limit 10 µM. applied products currently well spiked samples order evaluate performance sensitivity, accuracy, reproducibility.

Language: Английский

Citations

6
Point-of-care blood tests using a smartphone-based colorimetric analyzer for health check-up DOI
Suticha Chunta, Purim Jarujamrus, Akarapong Prakobkij

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(7)

Published: June 18, 2024

Language: Английский

Citations

5
Application of smart-responsive hydrogels in nucleic acid and nucleic acid-based target sensing: A review DOI

Meiqi Song,

Jinghui Zhang,

Ke Shen

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 267, P. 116803 - 116803

Published: Sept. 19, 2024

Language: Английский

Citations

3
Polyoxometalate-induced hydrogel with in situ nano/bioenzyme cascade reaction generating intensive and long-lasting glow-type chemiluminescence DOI
Habib Ullah Khan,

Jinlan Yang,

Meng Wang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 156129 - 156129

Published: Sept. 1, 2024

Language: Английский

Citations

2
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle DOI Creative Commons
Huiying Lu, Xiaofeng Wang

BioTechniques, Journal Year: 2024, Volume and Issue: 76(9), P. 415 - 423

Published: Aug. 5, 2024

Detecting glucose accurately and sensitively from clinical samples like tears saliva is still difficult. We have created a sensor that can detect with high sensitivity accuracy by combining the use of oxidase (GOx) to catalyze glucose, pistol-like DNAzyme (PLDz) transform signal, gold nanoparticles (AuNPs) enhance optical properties exonuclease-III (Exo-III) amplify signal. As result, proposed method exhibits low detection limit 7.5 pM wide range covering seven orders magnitude. The suggested dual-mode strategy provides sensitive, precise specific for glucose. Another advantage technique significantly improves precision consistency measurements, demonstrating its immense potential in biomedical research diagnostics.

Language: Английский

Citations

0
Optical Image Sensors for Smart Analytical Chemiluminescence Biosensors DOI Creative Commons
Reza Abbasi,

Xinyue Hu,

Alain Zhang

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(9), P. 912 - 912

Published: Sept. 12, 2024

Optical biosensors have emerged as a powerful tool in analytical biochemistry, offering high sensitivity and specificity the detection of various biomolecules. This article explores advancements integration optical with microfluidic technologies, creating lab-on-a-chip (LOC) platforms that enable rapid, efficient, miniaturized analysis at point need. These LOC leverage phenomena such chemiluminescence electrochemiluminescence to achieve real-time quantification analytes, making them ideal for applications medical diagnostics, environmental monitoring, food safety. Various detectors used detecting are reviewed, including single-point photomultiplier tubes (PMT) avalanche photodiodes (APD), pixelated charge-coupled devices (CCD) complementary metal-oxide-semiconductor (CMOS) sensors. A significant advancement discussed this review is image sensors, particularly CMOS sensors provide numerous advantages over traditional detectors, high-resolution imaging, spatially resolved measurements, ability simultaneously detect multiple analytes. Their compact size, low power consumption, cost-effectiveness further enhance their suitability portable point-of-care diagnostic devices. In future, machine learning algorithms these technologies promises data interpretation, driving development more sophisticated, accessible tools diverse applications.

Language: Английский

Citations

0