Materials,
Journal Year:
2023,
Volume and Issue:
16(17), P. 5898 - 5898
Published: Aug. 29, 2023
Tissue-engineered
bone
tissue
grafts
are
a
promising
alternative
to
the
more
conventional
use
of
natural
donor
grafts.
However,
choosing
an
appropriate
biomaterial/scaffold
sustain
cell
survival,
proliferation,
and
differentiation
in
3D
environment
remains
one
most
critical
issues
this
domain.
Recently,
chitosan/gelatin/genipin
(CGG)
hybrid
scaffolds
have
been
proven
as
suitable
induce
osteogenic
commitment
undifferentiated
cells
when
doped
with
graphene
oxide
(GO).
Some
concern
is,
however,
raised
towards
graphene-related
material
medical
applications.
The
purpose
work
was
thus
check
if
potential
CGG
without
added
GO
could
be
increased
by
improving
medium
diffusion
culture
differentiating
cells.
To
aim,
level
extracellular
matrix
(ECM)
mineralization
evaluated
human
bone-marrow-derived
stem
(hBMSC)-seeded
upon
under
perfusion
flow
dedicated
custom-made
bioreactor
system.
One
week
after
initiating
dynamic
culture,
histological/histochemical
evaluations
were
carried
out
analyze
early
culture.
analyses
show
enhanced
ECM
perfused
compared
static
counterpart.
results
investigation
reveal
new
perspective
on
efficient
clinical
applications
GO.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(10), P. 5219 - 5219
Published: May 10, 2024
Interrupted
blood
flow
in
the
brain
due
to
ischemic
injuries
such
as
stroke
or
traumatic
injury
results
irreversible
damage,
leading
cognitive
impairment
associated
with
inflammation,
disruption
of
blood-brain
barrier
(BBB),
and
cell
death.
Since
BBB
only
allows
entry
a
small
class
drugs,
many
drugs
used
treat
ischemia
other
tissues
have
failed
brain-related
disorders.
The
administration
mesenchymal
stem
(MSC)-derived
extracellular
vesicles
(EVs)
has
shown
promise
improving
functional
recovery
following
cerebral
by
inducing
vessel
formation.
To
facilitate
treatment
approach,
it
is
necessary
develop
bioprocesses
that
can
produce
therapeutically
relevant
MSC-EVs
reproducible
scalable
manner.
This
study
evaluated
feasibility
using
stirred
suspension
bioreactors
(SSBs)
scale-up
serum-free
production
pro-angiogenic
under
clinically
physioxic
conditions.
It
was
found
MSCs
grown
SSBs
generated
EVs
stimulated
angiogenesis
microvascular
endothelial
cells,
supporting
use
for
application
ischemia.
These
properties
were
impaired
at
higher
confluency,
outlining
importance
considering
time
harvest
when
developing
manufacture
EV
populations.
Cytotherapy,
Journal Year:
2024,
Volume and Issue:
26(12), P. 1532 - 1546
Published: July 3, 2024
Human
mesenchymal
stromal
cells
(hMSCs)
are
a
naturally
adherent
cell
type
and
one
of
the
most
studied
cellular
agents
used
in
therapy
over
last
20
years.
Their
mechanism
action
has
been
primarily
associated
with
paracrine
signalling,
which
contributed
to
an
increase
number
studies
focused
on
hMSC-related
extracellular
vesicles
(EVs).
In
this
study,
we
demonstrate
for
first
time
that
human
telomerase
reverse
transcriptase
(hTERT)
immortalised
hMSCs
can
be
adapted
suspension
culture,
eliminating
need
microcarriers
or
other
matrixes
support
growth.
This
novel
line,
named
(S-hMSCs),
doubling
approximately
55
hours,
growth
rate
0.423
d−1.
Regarding
its
immunophenotype
characteristics,
S-hMSCs
retained
close
90%
CD73
CD105
expression
levels,
CD90
receptor
being
downregulated
during
adaptation
process.
An
RNA
sequencing
analysis
showed
upregulation
transcripts
coding
CD44,
CD46
CD47
compared
levels
AT-hMSCs
hTERT-hMSCs.
The
line
herein
established
was
able
generate
EVs
using
chemically
defined
medium
formulation
these
nanoparticles
averaging
150
nm
size
displaying
markers
CD63,
CD81,
TSG101,
while
not
expressing
negative
marker
calnexin.
body
evidence,
combined
visual
confirmation
EV
presence
transmission
electron
microscopy,
demonstrates
EV-producing
capabilities
S-hMSCs.
provides
platform
process
development,
drug
discovery
translational
field.
Tissue Engineering Part A,
Journal Year:
2023,
Volume and Issue:
29(23-24), P. 607 - 619
Published: Aug. 11, 2023
Severe
acute
pancreatitis
(SAP)
is
a
common
abdominal
emergency
with
high
mortality
rate
and
lack
of
effective
therapeutic
options.
Although
mesenchymal
stem
cell
(MSC)
transplantation
potential
treatment
for
SAP,
the
mechanism
remains
unclear.
It
has
been
suggested
that
MSCs
may
act
mainly
through
paracrine
effects;
therefore,
we
aimed
to
demonstrate
efficacy
extracellular
vesicles
(EVs)
derived
from
human
umbilical
cord
cells
(UCMSCs)
SAP.
Na-taurocholate
was
used
induce
rat
SAP
model
retrograde
injection
into
biliopancreatic
duct.
After
72
h
EVs
transplantation,
pancreatic
pathological
damage
alleviated,
along
decrease
in
serum
amylase
activity
pro-inflammatory
cytokine
levels.
Interestingly,
when
UCMSCs
were
preconditioned
10
ng/mL
tumor
necrosis
factor
alpha
(TNF-α)
48
h,
obtained
(named
TNF-α-EVs)
performed
an
enhanced
efficacy.
Furthermore,
both
animal
cellular
experiments
showed
TNF-α-EVs
alleviated
necroptosis
acinar
RIPK3/MLKL
axis.
In
conclusion,
our
study
demonstrated
able
enhance
effect
on
by
inhibiting
compared
normal
EVs.
This
heralds
be
promising
approach
future.
Mesenchymal
(MSCs)
are
ideal
seed
repairing
many
diseases.
Paracrine
secretion
important
pathway
achieve
effects,
MSC-derived
have
shown
provide
similar
functions
MSCs.
However,
severe
this
study,
(UCMSC)-derived
injury
cells.
More
importantly,
exerted
The
outcomes
work
evidence
as
cell-free
therapy
strategy
Materials,
Journal Year:
2023,
Volume and Issue:
16(17), P. 5898 - 5898
Published: Aug. 29, 2023
Tissue-engineered
bone
tissue
grafts
are
a
promising
alternative
to
the
more
conventional
use
of
natural
donor
grafts.
However,
choosing
an
appropriate
biomaterial/scaffold
sustain
cell
survival,
proliferation,
and
differentiation
in
3D
environment
remains
one
most
critical
issues
this
domain.
Recently,
chitosan/gelatin/genipin
(CGG)
hybrid
scaffolds
have
been
proven
as
suitable
induce
osteogenic
commitment
undifferentiated
cells
when
doped
with
graphene
oxide
(GO).
Some
concern
is,
however,
raised
towards
graphene-related
material
medical
applications.
The
purpose
work
was
thus
check
if
potential
CGG
without
added
GO
could
be
increased
by
improving
medium
diffusion
culture
differentiating
cells.
To
aim,
level
extracellular
matrix
(ECM)
mineralization
evaluated
human
bone-marrow-derived
stem
(hBMSC)-seeded
upon
under
perfusion
flow
dedicated
custom-made
bioreactor
system.
One
week
after
initiating
dynamic
culture,
histological/histochemical
evaluations
were
carried
out
analyze
early
culture.
analyses
show
enhanced
ECM
perfused
compared
static
counterpart.
results
investigation
reveal
new
perspective
on
efficient
clinical
applications
GO.
