Biosensors,
Journal Year:
2025,
Volume and Issue:
15(6), P. 354 - 354
Published: June 4, 2025
Nanoquenchers
with
a
single
quenching
cofactor
exhibit
limited
fluorescence
efficiency.
In
this
work,
metal–organic
hybrid
dual
cofactors
(Cu2+
and
pyrroloquinoline
quinone
or
PQQ)
was
prepared
by
metal-coordinated
assembly
used
as
nanoquencher
for
protease
assay
enhanced
The
peptide
substrate
an
oligohistidine
(His6)
tag
labeled
fluorophore.
Caspase-3
determined
example.
attached
onto
the
surface
of
Cu-PQQ
metal
coordination
interaction
between
unsaturated
Cu2+
on
nanoparticle
His6
in
peptide.
cleavage
enzymatic
hydrolysis
led
to
release
fluorophore-conjugated
segment
from
surface,
thus
turning
fluorescence.
nanoprobe
determine
caspase-3
linear
range
0.01–5
ng/mL
detection
limit
7
pg/mL.
Furthermore,
method
evaluate
inhibition
efficiency
monitor
drug-induced
cell
apoptosis.
contrast
other
means
immobilization,
such
physical
adsorption
covalent
coupling,
strategy
based
is
simple
powerful,
thereby
achieving
assays
activity
lysates
satisfactory
result.
work
should
be
valuable
design
nanoquenchers
multiple
development
novel
biosensors.
Exploration,
Journal Year:
2024,
Volume and Issue:
4(6)
Published: March 22, 2024
Abstract
Metals
are
an
emerging
topic
in
cancer
immunotherapy
that
have
shown
great
potential
modulating
immunity
cycle
and
promoting
antitumor
by
activating
the
intrinsic
immunostimulatory
mechanisms
which
been
identified
recent
years.
The
main
challenge
of
metal‐assisted
lies
fact
free
metals
as
ion
forms
easily
cleared
during
circulation,
even
cause
systemic
metal
toxicity
due
to
off‐target
effects.
With
rapid
development
nanomedicine,
metal‐based
smart
nanosystems
(MSNs)
with
unique
controllable
structure
become
one
most
promising
delivery
carriers
solve
issue,
owing
their
various
endogenous/external
stimuli‐responsiveness
release
ions
for
metalloimmunotherapy.
In
this
review,
state‐of‐the‐art
research
progress
metal‐related
is
comprehensively
summarized.
First,
mainstream
MSNs‐assisted
will
be
delineated.
immunological
effects
certain
categorization
MSNs
different
characters
compositions
then
provided,
followed
representative
exemplar
applications
treatment,
synergistic
combination
immunotherapy.
Finally,
we
conclude
review
a
summary
remaining
challenges
associated
provide
authors'
perspective
on
further
advances.
Metal–organic
frameworks
(MOFs)
have
emerged
as
innovative
nanozyme
mimics,
particularly
in
the
area
of
oxidase
catalysis,
outperforming
traditional
MOF-based
peroxidase
and
other
nanomaterial-based
systems.
This
review
explores
various
advantages
that
MOFs
offer
terms
catalytic
activity,
low-cost,
stability,
structural
versatility.
With
a
primary
focus
on
their
application
biochemical
sensing,
oxidases
demonstrated
remarkable
utility,
prompting
thorough
exploration
design
modification
strategies.
Moreover,
aims
to
provide
comprehensive
analysis
strategies
employed
rational
MOF
structures
optimize
key
parameters
such
sensitivity,
selectivity,
stability
context
sensors.
Through
an
exhaustive
examination
recent
research
developments,
this
article
seeks
insights
into
nuanced
interplay
between
performance,
shedding
light
mechanisms
underpin
effectiveness
mimics.
Finally,
addresses
challenges
opportunities
associated
with
aiming
drive
further
advancements
structure
development
highly
effective
sensors
for
diverse
applications.
Advanced Powder Materials,
Journal Year:
2024,
Volume and Issue:
3(4), P. 100191 - 100191
Published: March 18, 2024
Nanozymes,
a
category
of
nanomaterials
endowed
with
enzyme-mimicking
capabilities,
have
exhibited
considerable
potential
across
diverse
application
domains.
This
comprehensive
review
delves
into
the
intricacies
regulating
nanozymes
through
N
elements,
elucidating
mechanisms
governing
element
control
in
design
and
these
nanomaterials.
The
initial
sections
introduce
foundational
background
significance
nanozymes.
Subsequent
exploration
detailed
discussion
regulation
on
nanozymes,
encompassing
vacancies,
doping,
coordination,
nitride.
These
regulatory
pathways
play
an
instrumental
role
fine-tuning
catalytic
activity
specificity
further
scrutinizes
practical
applications
spanning
sensing
detection,
infection
therapy,
tumor
pollutant
degradation.
In
conclusion,
it
succinctly
summarizes
current
research
findings
proposes
future
directions
for
development.
thorough
investigation
by
elements
anticipates
precise
over
their
performance,
thereby
advancing
extensive
utilization
realms
biomedical
environmental
applications.
Nanoscale Advances,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Composite
nanozymes
are
composed
of
enzymes
with
similar
or
different
catalytic
capabilities
and
have
higher
activity
than
a
single
enzyme.
In
recent
years,
composite
emerged
as
novel
nanomaterial
platforms
for
multiple
applications
in
various
research
fields,
where
they
used
to
produce
oxygen,
consume
glutathione,
toxic
reactive
oxygen
species
(ROS)
cancer
therapy.
The
therapeutic
approach
using
is
known
chemo-dynamic
therapy
(CDT).
Some
also
show
special
photothermal
conversion
effects,
enabling
them
be
combined
pioneering
treatments,
such
photodynamic
(PDT),
(PTT)
sonodynamic
(SDT),
enhance
the
anti-cancer
effects.
this
study,
classification
performances
reviewed,
along
their
advantages
synthesis
methods.
Furthermore,
treatment
cancers
emphasized,
prospective
challenges
future
discussed.
