An Overview on the Big Players in Bone Tissue Engineering: Biomaterials, Scaffolds and Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3836 - 3836

Published: March 29, 2024

Presently, millions worldwide suffer from degenerative and inflammatory bone joint issues, comprising roughly half of chronic ailments in those over 50, leading to prolonged discomfort physical limitations. These conditions become more prevalent with age lifestyle factors, escalating due the growing elderly populace. Addressing these challenges often entails surgical interventions utilizing implants or grafts, though treatments may entail complications such as pain tissue death at donor sites for along immune rejection. To surmount challenges, engineering has emerged a promising avenue injury repair reconstruction. It involves use different biomaterials development three-dimensional porous matrices scaffolds, alongside osteoprogenitor cells growth factors stimulate natural regeneration. This review compiles methodologies that can be used develop are important replacement Biomaterials orthopedic implants, several scaffold types production methods, well techniques assess biomaterials’ suitability human use—both laboratory settings within living organisms—are discussed. Even researchers have had some success, there is still room improvements their processing techniques, especially ones make scaffolds mechanically stronger without weakening biological characteristics. Bone therefore area rise bone-related injuries.

Language: Английский

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Melt Electrowriting for Biomimetic Tissue Engineering: Advances in Scaffold Design, Materials, and Multifunctional Applications

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(1)

Published: Jan. 1, 2025

ABSTRACT Melt electrowriting (MEW) is an emerging 3D printing technology that can precisely fabricate micro‐nano fibrous scaffolds and has significant application prospects in tissue engineering. This paper reviews the latest progress of MEW engineering scaffold fabrication, including its working principle, key design factors, various processable biomaterials. The enhancement function through filler addition, post‐treatment modification, combining with other manufacturing technologies are discussed. bone, cartilage, blood vessel, nerve, periodontal analyzed. Finally, challenges faces, such as material limitations, equipment reliability, process complexity, highlighted, future development directions to promote wide biomedical fields proposed.

Language: Английский

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Innovative 3D printing technologies and advanced materials revolutionizing orthopedic surgery: current applications and future directions

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 11, 2025

Three-dimensional (3D) printing has rapidly become a transformative force in orthopedic surgery, enabling the creation of highly customized and precise medical implants surgical tools. This review aims to provide more systematic comprehensive perspective on emerging 3D technologies—ranging from extrusion-based methods bioink powder bed fusion—and broadening array materials, including bioactive agents cell-laden inks. We highlight how these technologies materials are employed fabricate patient-specific implants, guides, prosthetics, advanced tissue engineering scaffolds, significantly enhancing outcomes patient recovery. Despite notable progress, field faces challenges such as optimizing mechanical properties, ensuring structural integrity, addressing regulatory complexities across different regions, considering environmental impacts cost barriers, especially low-resource settings. Looking ahead, innovations smart functionally graded (FGMs), along with advancements bioprinting, hold promise for overcoming obstacles expanding capabilities orthopedics. underscores pivotal role interdisciplinary collaboration ongoing research harnessing full potential additive manufacturing, ultimately paving way effective, personalized, durable solutions that improve quality life.

Language: Английский

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Integrating Melt Electrowriting and Fused Deposition Modeling to Fabricate Hybrid Scaffolds Supportive of Accelerated Bone Regeneration

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(3)

Published: Nov. 7, 2023

Emerging additive manufacturing (AM) strategies can enable the engineering of hierarchal scaffold structures for guiding tissue regeneration. Here, advantages two AM approaches, melt electrowriting (MEW) and fused deposition modelling (FDM), are leveraged integrated to fabricate hybrid scaffolds large bone defect healing. MEW is used a microfibrous core guide healing, while FDM stiff outer shell mechanical support, with constructs being coated pro-osteogenic calcium phosphate (CaP) nano-needles. Compared alone, prevent soft collapse into region support increased vascularization higher levels new formation 12 weeks post-implantation. In an additional group, also functionalized BMP2 via binding CaP coating, which further accelerates healing facilitates complete bridging defects after weeks. Histological analyses demonstrate that such well-defined annular bone, open medullary cavity, smooth periosteal surface, no evidence abnormal ectopic formation. These results potential integrating different approaches development regenerative biomaterials, in particular, enhanced outcomes possible MEW-FDM constructs.

Language: Английский

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Engineering complex tissue-like microenvironments with biomaterials and biofabrication

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(10), P. 1241 - 1257

Published: April 23, 2024

Advances in tissue engineering for both system modeling and organ regeneration depend on embracing recapitulating the target tissue's functional structural complexity. Microenvironmental features such as anisotropy, heterogeneity, other biochemical mechanical spatiotemporal cues are essential regulating development function. Novel biofabrication strategies innovative biomaterial design have emerged promising tools to better reproduce features. These facilitate a transition towards high-fidelity biomimetic structures, offering opportunities deeper understanding of function superior therapies. In this review, we explore some key compositional aspects tissues, lay out how achieve similar outcomes with current fabrication strategies, identify main challenges avenues future research.

Language: Английский

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Nanostructured polymer composites for bone and tissue regeneration

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137834 - 137834

Published: Nov. 1, 2024

Language: Английский

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Structurally Defined Cartilaginous MEW-Assembloids for Critical-size Long Bone Healing

Biomaterials, Journal Year: 2025, Volume and Issue: 319, P. 123202 - 123202

Published: Feb. 19, 2025

Language: Английский

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Enhancing Printing accuracy of Arbitrarily Non-linear Fibers in Melt Electrowriting: Role of Inertia and Charge Effect

Additive manufacturing, Journal Year: 2025, Volume and Issue: unknown, P. 104767 - 104767

Published: April 1, 2025

Language: Английский

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3D printing of damage‐tolerant bone scaffolds with bioinspired hierarchical porous structures

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Abstract Tissue engineering scaffolds with hierarchical porous structures has been a promising strategy for healing large bone defects. The fabrication of multiscale resembling real tissues presents great challenge. In addition, as the porosity increases, mechanical strength decreases dramatically, leading to catastrophic failure under external loadings. current work, cryogenic 3D printing technique fabricating hydroxyapatite (HA) and polyether ether ketone (PEEK) composite was proposed. Both pure HA HA/PEEK ranging from 60% 80% were successfully fabricated. Submillimeter scale pores (∼300 µm), microscale (∼10 nanopores (∼500 nm) integrated in 3D‐printed scaffolds. Because hierarchically arrangement pores, printed show an unusual long plateau strain during compressions, indicating excellent damage tolerance. scaffold can withstand compression strains up without disintegration, showing even better resistance. Compared traditional ceramic scaffolds, extraordinary specific tolerance by keeping high volume percentage at same time. degradation capability nanoparticles vitro cellular activity testing further demonstrates potential application tissue repair.

Language: Английский

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Advancements in Musculoskeletal Tissue Engineering: The Role of Melt Electrowriting in 3D-Printed Scaffold Fabrication

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(5), P. 163 - 163

Published: May 7, 2025

Musculoskeletal tissue injuries of the bone, cartilage, ligaments, tendons, and skeletal muscles are among most common experienced in medicine become increasingly problematic cases significant damage, such as nonunion bone defects volumetric muscle loss. Current gold standard treatment options for musculoskeletal injuries, although effective, have limited capability to fully restore native structure function. To overcome this challenge, three-dimensional (3D) printing techniques emerged promising therapeutic regeneration. Melt electrowriting (MEW), a recently developed advanced 3D technique, has gained traction field regeneration because its ability fabricate complex customizable scaffolds via high-precision microfiber deposition. The tailorability at microscale levels offered by MEW allows enhanced recapitulation microenvironment. Here, we survey recent contributions advancing engineering. More specifically, briefly discuss principles technical aspects MEW, provide an overview current printers on market, review in-depth latest biomedical applications regeneration, and, lastly, examine limitations offer future perspectives.

Language: Английский

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