On-chip fabrication of tailored 3D hydrogel scaffolds to model cancer cell invasion and interaction with endothelial cells DOI Creative Commons
Federico Cantoni, Laurent Barbe, Ananya Roy

et al.

APL Bioengineering, Journal Year: 2024, Volume and Issue: 8(4)

Published: Dec. 1, 2024

The high mortality associated with certain cancers can be attributed to the invasive nature of tumor cells. Yet, complexity studying invasion hinders our understanding how spreads. This work presents a microengineered three-dimensional (3D) in vitro model for cancer cell and interaction endothelial was generated by printing biomimetic hydrogel scaffold directly on chip using 2-photon polymerization that simulates brain's extracellular matrix. scaffold's geometry specifically designed facilitate growth continuous layer cells one side, while also allowing introduction other side. arrangement confines spatially enables situ microscopy as they invade interact layer. We examined impact 3D parameters hydrogel's physical properties used patient derived glioblastoma study their effect invasion. Notably, tended infiltrate faster when an barrier present. potential adjusting properties, coupled capability real-time observation tumor-endothelial interactions, offers platform tumor–endothelial interactions.

Language: Английский

Engineering Multifunctional Surface Topography to Regulate Multiple Biological Responses DOI Creative Commons

Mohammad Asadi Tokmedash,

Changheon Kim,

Ajay P Chavda

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123136 - 123136

Published: Jan. 1, 2025

Language: Английский

Citations

2
Recent advances in 3D bioprinting for cancer research: From precision models to personalized therapies DOI

Ruchika,

Neha Bhardwaj, Sudesh Kumar Yadav

et al.

Drug Discovery Today, Journal Year: 2024, Volume and Issue: 29(4), P. 103924 - 103924

Published: Feb. 22, 2024

Language: Английский

Citations

10
Radiotherapy Resistance of 3D Bioprinted Glioma via ITGA2/p‐AKT Signaling Pathway DOI
Dongdong Liu,

Haotian Tian,

Huaixu Li

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(9)

Published: Jan. 30, 2024

Due to the inherent radiation tolerance, patients who suffered from glioma frequently encounter tumor recurrence and malignant progression within target area, ultimately succumbing treatment ineffectiveness. The precise mechanism underlying tolerance remains elusive due dearth of in vitro models limitations associated with animal models. Therefore, a bioprinted model is engineered, characterized phenotypic traits vitro, compared 2D ones when subjected X-ray assessed. By comparing differential gene expression profiles between 3D model, identify functional genes, analyze distinctions patterns. Results showed that exhibited substantial alterations genes stromal microenvironment, notably significant increase ITGA2 (integrin subunit A2). In models, knockdown via shRNA resulted reduced cells concomitant inhibition p-AKT pathway. Overall, faithfully recapitulates vivo microenvironment (TME) exhibits enhanced resistance radiation, mediated through ITGA2/p-AKT This represents superior platform for investigating radiotherapy tolerance.

Language: Английский

Citations

5
Micro‐Vessels‐Like 3D Scaffolds for Studying the Proton Radiobiology of Glioblastoma‐Endothelial Cells Co‐Culture Models DOI Creative Commons

Qais Akolawala,

Floor Keuning,

Marta Rovituso

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(6)

Published: Nov. 10, 2023

Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. While X-ray radiotherapy and chemotherapy remain current standard, proton beam therapy appealing alternative as protons can damage cells while sparing surrounding healthy tissue. However, effects on in vitro GBM models at cellular level, especially when co-cultured endothelial cells, building blocks micro-vessels, are still unexplored. In this work, novel 3D-engineered scaffolds inspired by geometry microvasculature designed, where cluster proliferate. The architectures fabricated two-photon polymerization (2PP), pre-cultured (HUVECs), then cultured human cell line (U251). micro-vessel structures enable vivo-like morphologies, results show higher DNA double-strand breakage monoculture samples compared to U251/HUVECs co-culture, 2D featuring larger number foci 3D. discrepancy terms radiation response indicates difference radioresistance mediated presence HUVECs possible induction stemness features that contribute improved repair.

Language: Английский

Citations

12
Engineered Cell Microenvironments: A Benchmark Tool for Radiobiology DOI Creative Commons

Qais Akolawala,

Angelo Accardo

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

The development of engineered cell microenvironments for fundamental mechanobiology, in vitro disease modeling, and tissue engineering applications increased exponentially during the last two decades. In such context, radiobiology is a field research aiming at understanding effects ionizing radiation (e.g., X-rays/photons, high-speed electrons, protons) on biological (cancerous) tissues cells, particular terms DNA damage leading to death. Herein, perspective provides comparative assessment overview scaffold-free, scaffold-based, organ-on-a-chip models radiobiology, highlighting opportunities, limitations, future pathways improve currently existing approaches toward personalized cancer medicine.

Language: Английский

Citations

0
Smart Polymeric 3D Microscaffolds Hosting Spheroids for Neuronal Research via Quantum Metrology DOI Creative Commons
Beatriz N. L. Costa, Ana Marote, Catarina Barbosa

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Toward the aim of reducing animal testing, innovative in vitro models are required. Here, this study proposes a novel smart polymeric microscaffold to establish an advanced 3D model dopaminergic neurons. These scaffolds fabricated with Ormocomp via Two-Photon Polymerization. The further enhanced by functionalization fluorescent nanodiamonds (FNDs), which can serve as quantum nanosensors for both magnetic and temperature sensing. material biocompatibility is tested using two different cell lines, SH-SY5Y A431, viability over 98%. A total 69% FNDs grafted on structure compared those that remained glass surface. Cells several microenvironments, final assembly required metrology experiments achieved 91% biocompatibility. Subsequently, embryoid bodies containing neurons, type affected Parkinson's disease (PD), integrated FND-functionalized scaffolds. This successfully established, demonstrated strong interactions between neurons scaffold, directional growth neurites along scaffold. Ultimately, have developed platform enables readout signaling holds great potential future PD research.

Language: Английский

Citations

0
Multiphoton 3D lithography DOI
Edvinas Skliutas, Greta Merkininkaitė, Shoji Maruo

et al.

Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)

Published: March 13, 2025

Language: Английский

Citations

0
Nano‐Topography Enhanced Topological‐Cell‐Analysis in Radiation‐Therapy DOI Creative Commons
Francesca Pagliari,

Maria‐Francesca Spadea,

Pierre Montay‐Gruel

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract Radiotherapy (RT) is a cancer treatment technique that involves exposing cells to ionizing radiation, including X‐rays, electrons, or protons. RT offers promise treat cancer, however, some inherent limitations can hamper its performance. Radio‐resistance, whether innate acquired, refers the ability of tumor withstand treatment, making it key factor in failure. This perspective hypothesizes nanoscale surface topography impact on topology network under and this understanding possibly advance assessment cell radio‐resistance applications. An experimental plan proposed test hypothesis, using exposed various forms. By examining influence 2D 3D scaffold architecture cells, approach diverges from traditional methodologies, such as clonogenic assays, offering novel viewpoint integrates fields tissue engineering, artificial intelligence, nanotechnology. The hypotheses at base not only may but also insights into broader field structural biology. Nanotechnology label‐free Raman phenotyping biological samples are lenses through which scientists better elucidate structure‐function relationship systems.

Language: Английский

Citations

0
Three-dimensional bioprinted in vitro glioma tumor constructs for synchrotron microbeam radiotherapy dosimetry and biological study using gelatin methacryloyl hydrogel DOI Creative Commons
John Paul O Bustillo, E. Engels,

Vincent de Rover

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 22, 2025

Language: Английский

Citations

0
Deciphering the Influence of Effective Shear Modulus on Neuronal Network Directionality and Growth Cones’ Morphology via Laser‐Assisted 3D‐Printed Nanostructured Arrays DOI Creative Commons
George Flamourakis,

Qiangrui Dong,

Dimitri Kromm

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract In the present study, influence of topographic and mechanical cues on neuronal growth cones (NGCs) network directionality in 3D‐engineered cell culture models is explored. Two‐photon polymerization (2PP) employed to fabricate nanopillar arrays featuring tunable effective shear modulus. Large variations properties are obtained by altering aspect ratio nanostructures. The seeded with different lines, including neural progenitor cells (NPCs) derived from human induced pluripotent stem (iPSCs), I 3 Neurons, primary hippocampal neurons. All types exhibit preferential orientations according topology, as shown neurites creating a high number oriented orthogonal networks. Furthermore, differentiation maturation NPCs affected nanopillars, expression mature marker Synapsin I. Lastly, NGCs influenced modulus terms spreading area, stochastic optical reconstruction microscopy (STORM) assess cytoskeleton organization at nanometric resolution. developed approach, involving laser‐assisted 3D microfabrication, neuro‐mechanobiology, super‐resolution microscopy, paves way for prospective comparative studies evolution networks healthy diseased (e.g., neurodegenerative) conditions.

Language: Английский

Citations

2