Protein-Polyelectrolyte Complexation – Effects of Sterically Repulsive Groups, Macromolecular Architecture and Hierarchical Assembly DOI Creative Commons
Raman Hlushko, Alexander Marin, Alexander K. Andrianov

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Self-assembly of proteins and polyelectrolytes in aqueous solutions is a promising approach for the development advanced biotherapeutics engineering efficient biotechnological processes. Synthetic polyions containing sterically repulsive ethylene oxide moieties are especially attractive as protein modifying agents, they can potentially induce PEGylation-like stabilizing effect without need complex covalent binding reactions. In this study, we investigated protein-binding properties anionic based on an inorganic polyphosphazene backbone, with groups incorporated into both grafted linear macromolecular topologies. The study was conducted using isothermal titration calorimetry, dynamic light scattering, cryogenic electron microscopy to analyze samples their vitrified state. Our findings revealed that stability resulting protein-polyion complexes thermodynamic profiles these interactions were influenced by molecular architecture polyions. Furthermore, formation hierarchical assemblies polyions, through ionic crosslinking nanogels, rapidly reduced or eliminated ability polyelectrolyte bind proteins. comprehensive analysis, combining thermodynamic, spectroscopy direct visualization techniques, provides valuable insights multivalent charge-charge critical successful non-covalent modification methods.

Language: Английский

Noble Metal Nanoparticle-Based Photothermal Therapy: Development and Application in Effective Cancer Therapy DOI Open Access

Shujie Yu,

Guoyu Xia,

Nan Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5632 - 5632

Published: May 22, 2024

Photothermal therapy (PTT) is a promising cancer modality with significant advantages such as precise targeting, convenient drug delivery, better efficacy, and minimal adverse effects. effectively absorbs the photothermal transducers in near-infrared region (NIR), which induces effect to work. Although PTT has role tumor therapy, it also suffers from low conversion efficiency, biosafety, incomplete elimination. Therefore, use of nanomaterials themselves photosensitizers, targeted modification improve targeting or combined other therapies can therapeutic effects reduce side Notably, noble metal have attracted much attention because they strong surface plasmon resonance an effective absorbance light at specific wavelengths. be used excellent photosensitizers mediate its efficiency. This paper provides comprehensive review key played by therapy. It describes major challenges encountered during implementation

Language: Английский

Citations

20
A Brain-Targeting NIR-II Polymeric Phototheranostic Nanoplatform toward Orthotopic Drug-Resistant Glioblastoma DOI
Xiang Su, Yi‐Sheng Liu, Yong Zhong

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: 25(9), P. 3445 - 3454

Published: Feb. 24, 2025

Glioblastoma is the most common and devastating brain tumor owing to its high invasiveness high-frequency drug resistance. Near infrared-II (NIR-II) imaging-guided phototherapy based on polymer luminogens provides a promising remedy against drug-resistant glioma, but it difficult maximize photoenergy utilization. Herein, we designed series of semiconducting polymers boost visualization ablation glioblastoma. By subtly engineering side chains or substituents phenothiazine thiophene moieties, an NIR-II luminogen with high-quality fluorescence performance, good solubility, superior photothermal conversion, balanced reactive oxygen species generation achieved. The optimal possesses branched alkyl chain tetraphenylethylene pendant manipulate equilibrium between radiative nonradiative energy-dissipating channels. High-sensitivity imaging was used monitor blood-brain barrier penetration glioma cell targeting apolipoprotein E-modified nanoparticles. NIR irradiation triggers maximizes photon utilization in prominent photodynamic/photothermal synergistic therapy orthotopic

Language: Английский

Citations

1
Emerging nanoparticle-based x-ray imaging contrast agents for breast cancer screening DOI
Katherine J. Mossburg,

Diego Barragan,

Nathaniel H. O

et al.

Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: April 22, 2025

Breast cancer is one of the most common types cancer, however, preventive screening has contributed to a significant reduction in mortality over past four decades. The first-line methods for breast such as mammography and tomosynthesis, are x-ray-based modalities. Unfortunately, their detection rates low patients with dense breasts. These, other high-risk women, now encouraged receive supplemental screening. imaging diverse, including ultrasound, MRI, nuclear imaging, X-ray-based modalities CT contrast-enhanced mammography/tomosynthesis. Due cost wide availability, see clinical use worldwide. These techniques benefit from contrast agents, which currently iodinated small molecules designed purposes. Consequently, developing new agents that specifically interest. This review describes these nanoparticle-based being researched enhanced performance. relevant parameters agent design evaluated, generation potential biointeractions. Iodinated discussed comparison. Nanoparticles covered include silver sulfide, telluride, gold, bismuth sulfide-based among others. Finally, perspectives on future developments this field offered.

Language: Английский

Citations

0
Advances in Ultrasmall Inorganic Nanoparticles for Nanomedicine: From Diagnosis to Therapeutics DOI
Jun Yong Oh,

Kathleen Elizabeth Villaseñor,

Andrea C Kian

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Inorganic nanoparticles possess unique physicochemical properties that make them attractive candidates for diverse applications in nanomedicine, including as contrast agents and therapeutics. However, many inorganic are composed of high-atomic-number elements, raising safety concerns due to potential long-term retention the body. ultrasmall (UINPs), i.e., those less than ∼5 nm diameter, can offer advantage rapid renal clearance from body, reducing toxicity risks associated with prolonged exposure thereby creating a path toward clinical translation. In this review, we discuss current knowledge on design functionalization UINPs, exploring their capabilities diagnosis therapeutics, examples radiosensitization, photothermal, anti-inflammatory catalytic therapies. addition, limitations, approaches taken solve progress UINPs Through discussion, aim provide comprehensive perspective how advancing field underscoring significantly improve bioimaging therapeutic outcomes.

Language: Английский

Citations

0
Sesame ball-like PTT nanoplatforms with fluorescence-background-free temperature sensing DOI

Biying Bao,

Jia Song, Jian Yang

et al.

Journal of Materials Chemistry C, Journal Year: 2024, Volume and Issue: 12(30), P. 11648 - 11658

Published: Jan. 1, 2024

Sesame ball-like ZGGO:Cr,Hf–Ag 2 S composite nanoplatforms with broad-spectrum bactericidal performance can be used as afterglow ratio nanothermometers to realize real-time temperature monitoring during therapy processes.

Language: Английский

Citations

0
Conversion of Ultrasmall Glutathione-Coated Silver Nanoparticles during Dispersion in Water into Ultrasmall Silver Sulfide Nanoparticles DOI Creative Commons

Natalie Wolff,

Oleg Prymak, Nataniel Białas

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(17), P. 1449 - 1449

Published: Sept. 5, 2024

Ultrasmall silver nanoparticles (2 nm) were prepared by reduction with sodium borohydride (NaBH4) and stabilized the ligand glutathione (a tripeptide: glycine–cysteine–glutamic acid). NMR spectroscopy optical (UV fluorescence) revealed that these particles initially consist of fluorescing nanoclusters, both glutathione. Over time, nanoclusters disappear only remain. Furthermore, capping eliminates hydrogen sulfide (H2S) from central cysteine is released nanoparticle surface as tripeptide glycine–dehydroalanine–glutamic acid. Hydrogen reacts core to form sulfide. After four weeks in dispersion at 4 °C, this process completed. These processes cannot be detected transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS), or differential centrifugal sedimentation (DCS) methods resolve mixture nature core. photoelectron showed mostly oxidized state core, Ag(+I), freshly aged nanoparticles. results demonstrate ultrasmall can undergo unnoticed changes considerably affect their chemical, physical, biological properties. In particular, are much more toxic against cells bacteria than because presence clusters.

Language: Английский

Citations

0
Protein-Polyelectrolyte Complexation – Effects of Sterically Repulsive Groups, Macromolecular Architecture and Hierarchical Assembly DOI Creative Commons
Raman Hlushko, Alexander Marin, Alexander K. Andrianov

et al.

Soft Matter, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Self-assembly of proteins and polyelectrolytes in aqueous solutions is a promising approach for the development advanced biotherapeutics engineering efficient biotechnological processes. Synthetic polyions containing sterically repulsive ethylene oxide moieties are especially attractive as protein modifying agents, they can potentially induce PEGylation-like stabilizing effect without need complex covalent binding reactions. In this study, we investigated protein-binding properties anionic based on an inorganic polyphosphazene backbone, with groups incorporated into both grafted linear macromolecular topologies. The study was conducted using isothermal titration calorimetry, dynamic light scattering, cryogenic electron microscopy to analyze samples their vitrified state. Our findings revealed that stability resulting protein-polyion complexes thermodynamic profiles these interactions were influenced by molecular architecture polyions. Furthermore, formation hierarchical assemblies polyions, through ionic crosslinking nanogels, rapidly reduced or eliminated ability polyelectrolyte bind proteins. comprehensive analysis, combining thermodynamic, spectroscopy direct visualization techniques, provides valuable insights multivalent charge-charge critical successful non-covalent modification methods.

Language: Английский

Citations

0