Advancing tumor microenvironment and lymphoid tissue research through 3D bioprinting and biofabrication DOI Creative Commons

Corrado Mazzaglia,

Yan Yan Shery Huang, Jacqueline D. Shields

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 115485 - 115485

Published: Dec. 1, 2024

Cancer progression is significantly influenced by the complex interactions within tumor microenvironment (TME). Immune cells, in particular, play a critical role infiltrating tumors from circulation and surrounding lymphoid tissues an attempt to control their spread. However, they often fail this task. Current vivo vitro preclinical models struggle fully capture these intricate affecting our ability understand immune evasion predict drugs behaviour clinic. To address challenge, biofabrication particularly 3D bioprinting has emerged as promising tool for modeling both system. Its incorporate multiple cell types into matrices, enable tissue compartmentalization with high spatial accuracy, integrate vasculature makes it valuable approach. Nevertheless, limited research focused on capturing tumor-immune interplay vitro. This review highlights composition significance of TME, architecture function tissues, innovative approaches vitro, while proposing concept extended TME.

Language: Английский

Tumor Microenvironment On‐A‐Chip and Single‐Cell Analysis Reveal Synergistic Stromal–Immune Crosstalk on Breast Cancer Progression DOI Creative Commons
Kalpana Ravi, Yining Zhang,

Lydia Sakala

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Solid tumors develop within a complex environment called the tumor microenvironment (TME), which is sculpted by presence of other cells, such as cancer-associated fibroblasts (CAFs) and immune cells like macrophages (Mφs). Despite orchestrate tumor-supportive through intricate interaction with components TME. However, specific mechanism this intercellular dialogue regulated not fully understood. To that end, development an organotypic 3D breast TME-on-a-chip (TMEC) model, integrated single-cell RNA sequencing analysis, reported to mechanistically evaluate progression triple-negative cancer (TNBC) in patient-derived CAFs Mφs. Extensive functional assays, including invasion morphometric characterization, reveal synergistic influence Mφs on cells. Furthermore, gene expression pathway enrichment analyses identify involvement KYNU gene, suggesting potential evasion kynurenine pathway. Lastly, pharmacological targeting identified investigated.

Language: Английский

Citations

1
Unravelling approaches to study macrophages: from classical to novel biophysical methodologies DOI Creative Commons
Polina Vishnyakova, Andrey Elchaninov, Timur Fatkhudinov

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19039 - e19039

Published: Feb. 20, 2025

Macrophages play crucial roles in immune responses and tissue homeostasis. Despite the fact that macrophages were described more than a century ago, they continue to be cells of intensive interest. Advanced understanding phenotypic diversity holds great promise for development cell-based therapeutic strategies. The introduction innovative approaches cell biology greatly enhances our ability investigate unique characteristics macrophages. review considers both classical methods study high-tech approaches, including single-cell sequencing, mass spectrometry, droplet microfluidics, scanning probe microscopy atomic force spectroscopy. This will valuable specialists beginning their experienced scientists seeking deepen at intersection biological physical sciences.

Language: Английский

Citations

0
The Microenvironment of Solid Tumors: Components and current challenges of Tumor-on-a-Chip models DOI
Ilva de Fátima Souza,

João Paulo de Jesus Vieira,

Elton Bonifácio

et al.

Tissue Engineering Part B Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: July 15, 2024

Solid tumors represent the most common type of cancer in humans and are classified into sarcomas, lymphomas, carcinomas based on originating cells. Among these, carcinomas, which arise from epithelial glandular cells lining body's tissues, prevalent. Around world, a significant increase incidence solid is observed during recent years. In this context, efforts to discover more effective treatments have led deeper understanding tumor microenvironment its components. Currently, interactions between elements being intensely investigated. Remarkable progress research noted, largely due development advanced vitro models, such as Tumor-on-a-Chip models that assist ultimately discovering new for specific cancer. The purpose paper provide review cell components, along with advances tumor-on-a-chip designed mimic tumors, offering perspective current state-of-the-art. Recent studies using kind microdevices reproduce allowed better treatments. Nevertheless, applications technology present some limitations must be overcome order achieve broad application by researchers looking knowledge strategies improve therapies.

Language: Английский

Citations

1
Tumor-on-chip platforms for breast cancer continuum concept modeling DOI Creative Commons

Anca-Narcisa Neagu,

Danielle Whitham, Pathea Bruno

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 2, 2024

Our previous article entitled "Proteomics and its applications in breast cancer", proposed a Breast Cancer Continuum Concept (BCCC), including Cell as well Proteomic Concept. cancer-on-chip (BCoC), cancer liquid biopsy-on-chip (BCLBoC), metastasis-on-chip (BCMoC) models successfully recapitulate reproduce

Language: Английский

Citations

1
Breaking the mold: 3D cell cultures reshaping the future of cancer research DOI Creative Commons
Sandra Cordeiro, Beatriz B P P Oliveira, Rúben Valente

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 26, 2024

Despite extensive efforts to unravel tumor behavior and develop anticancer therapies, most treatments fail when advanced clinical trials. The main challenge in cancer research has been the absence of predictive models, accurately mimicking tumoral processes response treatments. microenvironment (TME) shows several human-specific physical chemical properties, which cannot be fully recapitulated by conventional 2D cell cultures or

Language: Английский

Citations

1
Recapitulating the Cancer‐Immunity Cycle on a Chip DOI
Yujin Lee,

Jaehong Min,

Solbin Kim

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

The cancer-immunity cycle is a fundamental framework for understanding how the immune system interacts with cancer cells, balancing T cell recognition and elimination of tumors while avoiding autoimmune reactions. Despite advancements in immunotherapy, there remains critical need to dissect each phase cycle, particularly interactions among tumor, vasculature, within tumor microenvironment (TME). Innovative platforms such as organ-on-a-chip, organoids, bioprinting microphysiological systems (MPS) are increasingly utilized enhance these interactions. These meticulously replicate crucial aspects TME responses, providing robust study progression, evasion, therapeutic interventions greater physiological relevance. This review explores latest MPS technologies modeling various stages cancer-immune critically evaluating their applications limitations advancing dynamics guiding development next-generation immunotherapeutic strategies.

Language: Английский

Citations

0
Advancing tumor microenvironment and lymphoid tissue research through 3D bioprinting and biofabrication DOI Creative Commons

Corrado Mazzaglia,

Yan Yan Shery Huang, Jacqueline D. Shields

et al.

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 115485 - 115485

Published: Dec. 1, 2024

Cancer progression is significantly influenced by the complex interactions within tumor microenvironment (TME). Immune cells, in particular, play a critical role infiltrating tumors from circulation and surrounding lymphoid tissues an attempt to control their spread. However, they often fail this task. Current vivo vitro preclinical models struggle fully capture these intricate affecting our ability understand immune evasion predict drugs behaviour clinic. To address challenge, biofabrication particularly 3D bioprinting has emerged as promising tool for modeling both system. Its incorporate multiple cell types into matrices, enable tissue compartmentalization with high spatial accuracy, integrate vasculature makes it valuable approach. Nevertheless, limited research focused on capturing tumor-immune interplay vitro. This review highlights composition significance of TME, architecture function tissues, innovative approaches vitro, while proposing concept extended TME.

Language: Английский

Citations

0