Milk-Derived Extracellular Vesicles: A Novel Perspective on Comparative Therapeutics and Targeted Nanocarrier Application DOI Creative Commons
Muttiah Barathan, Sook Luan Ng, Yogeswaran Lokanathan

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1282 - 1282

Published: Nov. 15, 2024

Milk-derived extracellular vesicles (mEVs) are emerging as promising therapeutic candidates due to their unique properties and versatile functions. These play a crucial role in immunomodulation by influencing macrophage differentiation cytokine production, potentially aiding the treatment of conditions such bone loss, fibrosis, cancer. mEVs also have capacity modulate gut microbiota composition, which may alleviate symptoms inflammatory bowel diseases promote intestinal barrier integrity. Their potential drug delivery vehicles is significant, enhancing stability, solubility, bioavailability anticancer agents while supporting wound healing reducing inflammation. Additionally, bovine exhibit anti-aging protect skin cells from UV damage. As vaccine platforms, offer advantages including biocompatibility, antigen protection, ability elicit robust immune responses through targeted specific cells. Despite these applications, challenges persist, complex roles cancer, effective loading, regulatory hurdles, need for standardized production methods. Achieving high targeting specificity understanding long-term effects mEV-based therapies essential clinical translation. Ongoing research aims optimize mEV methods, enhance capabilities, conduct rigorous preclinical studies. By addressing challenges, hold revolutionize development delivery, ultimately improving outcomes across various medical fields.

Language: Английский

Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo DOI Creative Commons
Morteza Abyadeh, Shahab Mirshahvaladi, Sara Assar Kashani

et al.

Journal of Extracellular Biology, Journal Year: 2024, Volume and Issue: 3(6)

Published: June 1, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell‐to‐cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)‐derived obtained through different techniques: ultracentrifugation (UC), high‐speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS‐EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cell‐cell interactions extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Language: Английский

Citations

9
Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling DOI
Solène Lenoir, Elliot Thouvenot,

Giacomo Gropplero

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

ABSTRACT This study presents a novel method and device for the hydrodynamic production of extracellular vesicles (EVs) derived from biomimetic multicellular 3D spheroids, enabling high-throughput particle release that is 10 to 20 times higher than in non-stimulated conditions. The facilitates formation spheroids human mesenchymal stem cells (hMSCs), offering an all-in-one approach both spheroid generation EV release. Production are reduced just few hours, with yield further increased by alternating periods high flow recovery sequential approach. Using this system, we explored impact starvation conditions on protein cargo EVs, identifying distinct markers through proteomics. Specifically, stimulation enriched EVs plasma membrane-derived mitochondrial proteins, revealing divergent biogenesis pathways. Importantly, produced exhibited therapeutic properties, demonstrated effects wound healing, angiogenesis, anti-inflammatory responses, some showing enhanced efficacy under stimulation.

Language: Английский

Citations

0
Immunomodulation effects of collagen hydrogel encapsulating extracellular vesicles derived from calcium silicate stimulated-adipose mesenchymal stem cells for diabetic healing DOI Creative Commons

Yen-Hong Lin,

Yeh Chen,

En‐Wei Liu

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 27, 2025

Diabetic wounds are characterized by chronic inflammation, reduced angiogenesis, and insufficient collagen deposition, leading to impaired healing. Extracellular vesicles (EVs) derived from adipose-derived mesenchymal stem cells (ADSC) offer a promising cell-free therapeutic strategy, yet their efficacy immunomodulation can be enhanced through bioactivation. In this study, we developed calcium silicate (CS)-stimulated ADSC-derived EVs (CSEV) incorporated into hydrogels create sustained-release system for promoting diabetic wound CSEV exhibited protein content, surface marker expression, bioactive cargo enriched with pro-angiogenic anti-inflammatory factors. vitro, CSEV-loaded significantly reactive oxygen species production, promoted cell proliferation migration compared standard EV-loaded collagen. Cytokine profiling revealed the upregulation of cytokines extracellular matrix components, highlighting immunomodulatory regenerative potential. vivo, histological evaluation rabbit models treated superior reepithelialization organized indicating accelerated closure. These findings underscore potential as an innovative effective platform enhancing healing simultaneously addressing inflammation tissue regeneration.

Language: Английский

Citations

0
Apoptotic mesenchymal stem cells and their secreted apoptotic extracellular vesicles: therapeutic applications and mechanisms DOI Creative Commons

Ruoxuan Wang,

Jiao Fu, Jing He

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 21, 2025

Mesenchymal stem cells (MSCs), an accessible and less ethically controversial class of adult cells, have demonstrated significant efficacy in treating a wide range diseases both the preclinical clinical phases. However, we do not yet clear understanding mechanisms by which MSCs exert their therapeutic effects vivo. We found that transplanted go apoptotic fate within 24 h vivo irrespective route administration. Still, short-term survival affect long-term efficacy. An increasing number studies transplantation (ApoMSCs) show similar or even better than viable MSCs, including variety disease models such as inflammatory diseases, skin damage, bone organ etc. Although exact mechanism has to be explored, recent shown undergo apoptosis are phagocytosed phagocytes, thereby exerting immunomodulatory effects. The extracellular vesicles secreted ApoMSCs (MSC-ApoEVs) play role promoting immunomodulation, endogenous cell regeneration, angiogenesis due properties inheritance molecular characteristics from parental MSCs. On this basis, review aims deeply explore applications secretion MSC-ApoEVs, well challenges they face.

Language: Английский

Citations

0
Extracellular vesicles as dynamic biomarkers: Monitoring therapeutic responses and drug resistance in cancer DOI
Shaimaa A. Gouhar, Zakaria Y. Abd Elmageed, Sherien M. El‐Daly

et al.

International review of cell and molecular biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Genetic tools for investigating the life cycle of extracellular vesicles DOI
Wenyi Zheng, Samantha Roudi,

Houze Zhou

et al.

Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0
Challenges and Opportunities in Translating Extracellular Vesicles into Clinical Applications DOI

Ciro Tetta,

Giovanni Camussi

Stem Cells and Development, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

Citations

0
Extracellular vesicles in chronic kidney disease: diagnostic and therapeutic roles DOI Creative Commons

Yixuan Zheng,

Hui Wang, Xueying Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: March 13, 2024

Chronic kidney disease (CKD) is a progressive disorder characterized by structural and functional changes in the kidneys, providing global health challenge with significant impacts on mortality rates. Extracellular vesicles (EVs), are vital physiological pathological processes associated CKD. They have been shown to modulate key pathways involved renal injury, including inflammation, fibrosis, apoptosis, oxidative stress. Currently, application research of EVs diagnosis treatment CKD highly prevalent. However, there currently lack standardized guidelines for their application, various methodologies advantages limitations. Consequently, we present an comprehensive summary elucidating multifaceted involvement both aspects Furthermore, explore potential as biomarkers diverse therapeutic roles This review provides overview current state management

Language: Английский

Citations

2
Proteomic Profiling of Mesenchymal Stem Cell-Derived Extracellular Vesicles: Impact of Isolation Methods on Protein Cargo DOI Open Access
Morteza Abyadeh, Shahab Mirshahvaladi, Sara Assar Kashani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell-to-cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)-derived obtained through different techniques: ultracentrifugation (UC), high-speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS-EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cellLJcell interactions, extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Language: Английский

Citations

2
Extracellular Vesicles: Diagnostic and Therapeutic Applications in Cancer DOI Creative Commons
Maria Antonietta Di Bella, Simona Taverna

Biology, Journal Year: 2024, Volume and Issue: 13(9), P. 716 - 716

Published: Sept. 12, 2024

In recent years, knowledge of cell-released extracellular vesicle (EV) functions has undergone rapid growth. EVs are membrane vesicles loaded with proteins, nucleic acids, lipids, and bioactive molecules. Once released into the space, delivered to target cells that may go through modifications in physiological or pathological conditions. nano shuttles a crucial role promoting short- long-distance cell-cell communication. Comprehension mechanism regulates this process is benefit for both medicine basic science. Currently, attract immense interest precision nanomedicine their potential use diagnosis, prognosis, therapies. This review reports latest advances EV studies, focusing on nature features conventional emerging methodologies used separation, characterization, visualization. By searching an extended portion relevant literature, work aims give summary nanomedical applications EVs. Moreover, concerns require further studies before translation clinical discussed.

Language: Английский

Citations

2