Promoting Radial Repair in Rabbits through BMP-9 Overexpression in Adipose-Derived Stem Cells Loaded with ICA/nHAC/PLA DOI Creative Commons

Fang-Tian Xu,

Lin Wang,

Shuang-Yi Li

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Abstract Background Bone defects commonly cause delayed bone healing and local dysfunction. Both adipose-derived stem cells (ADSCs) icariin (ICA)-loaded nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) have been reported to promote osteoblast metabolism accelerate fracture healing. Aim The aim of this study was explore the mechanism ADSCs combined with ICA-loaded nHAC/PLA in regulating defects. Methods effects ICA BMP-9 (Bone morphogenetic protein-9) overexpression on cell viability osteogenic differentiation were measured by qRT-PCR, western blotting alkaline phosphatase (ALP) assay. Alizarin red staining examined formation mineralized nodules ADSCs. Finally, radial defect rabbit model established, administrated nHAC/PLA. new detected X-ray, immunohistochemistry Hematoxylin eosin staining. Results significantly increased ALP activity nodule ADSCs, that amplified ICA. This combination also upregulated expression markers (BMP-9, OCN, OPN, COL-1, BSP, Runx-2) influenced key signaling pathways (p38 MAPK, JNK, ERK). In vivo, treatment group showed enhanced repair. Conclusions Our findings indicate BMP-9-overexpressed scaffolds is effective promoting repair, offering a potential therapeutic strategy for Core tip promoted proliferation ADSCs. BMP-9 osteogenesis ADSCs

Language: Английский

Engineering Multifunctional Surface Topography to Regulate Multiple Biological Responses DOI Creative Commons

Mohammad Asadi Tokmedash,

Changheon Kim,

Ajay P Chavda

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123136 - 123136

Published: Jan. 1, 2025

Language: Английский

Citations

1
Enhancing Bone Healing through Immunological Microenvironment Modulation via a Smart-Responsive Multifunctional Therapeutic System DOI Creative Commons
Yu Wang, Huaiyuan Zhang, Tinglin Zhang

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: 297, P. 112252 - 112252

Published: Feb. 18, 2025

Language: Английский

Citations

1
Targeted Sonodynamic Therapy Platform for Holistic Integrative Helicobacter pylori Therapy DOI Creative Commons
Xiaojing Yin, Yongkang Lai, Xinyuan Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 12(2)

Published: Nov. 13, 2024

Helicobacter pylori (H. pylori) is a primary pathogen associated with gastrointestinal diseases, including gastric cancer. The increase in resistance to antibiotics, along the adverse effects caused by complicated medication protocols, has made eradication of H. more formidable challenge, necessitating alternative therapeutics. Herein, targeted nanoplatform reported based on sonodynamic therapy, chitosan-conjugated fucose loaded indocyanine green (ICG@FCS). It penetrates mucosa and homes through dual targeting mechanisms: molecular via physical ultrasound. Upon ultrasound activation, it generates singlet oxygen, effectively attacking planktonic bacteria, disrupting biofilms, facilitating clearance intracellular bacteria promoting autophagy, multidrug-resistant strains. ICG@FCS minimally affects gut microbiota aids repair. holistic integrative therapy strategy proposed that targets while preserving health. This emphasizes importance maintaining patient health eradicating pathogen. advancement set refine comprehensive antibacterial approach, offering promising horizon ongoing battle against antibiotic effective cancer prevention strategies.

Language: Английский

Citations

5
Magnesium‑phosphorus gel containing oxygen vacancy niobium oxide with photothermal property for treating tumor, preventing infection and facilitating bone generation DOI
Qinghui Zhao, Xu Xiao,

Tianhui Zheng

et al.

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 172, P. 214237 - 214237

Published: Feb. 20, 2025

Language: Английский

Citations

0
FePS3-Nanosheets-Integrated multifunctional nanocomposite hydrogel for multimodal synergistic osteosarcoma therapy and enhanced bone regeneration DOI
Bing Ye, Xirui Jing, Yanlin Su

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162175 - 162175

Published: March 1, 2025

Language: Английский

Citations

0
Innovative Approaches in Bone Tissue Engineering: Strategies for Cancer Treatment and Recovery DOI Open Access

Samiksha Khobragade,

Manish Deshmukh,

Ujwal Vyas

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3937 - 3937

Published: April 22, 2025

Cancer has rapidly emerged as a leading global cause of premature mortality, with significant economic implications projected to reach USD 25.2 trillion from 2020 2050. Among the various types cancer, primary bone cancers, though uncommon, are see nearly 4000 new cases diagnosed in United States 2024. The complexity treating cancer arises its rarity, diversity, and challenges associated surgical interventions, metastatic spread, post-operative complications. Advancements tissue engineering (BTE) have introduced innovative therapeutic approaches promote regeneration address tumor recurrence. This interdisciplinary field integrates biomaterials, scaffolds, gene therapy, utilizing technologies such 3D bioprinting create custom scaffolds that facilitate cellular activities essential for regeneration. Recent developments biodegradable, bioactive materials aim enhance biocompatibility effectiveness while nanotechnology presents promising avenues targeted drug delivery improved outcomes. review outlines current landscape BTE, highlighting scaffold fabrication techniques, advantages incorporating stem cell therapies, future directions, including integration artificial intelligence design personalized medicine orthopedic oncology. work underscores necessity ongoing research innovation, aiming improve strategies specifically designed unique posed by sarcomas cancers.

Language: Английский

Citations

0
Unlocking the future of osteoarthritis: Material engineering and drug delivery confluence for advanced therapeutic approaches DOI Creative Commons
Bhupendra Kumar, Laxmi Akhileshwar Jha, Prashant Pandey

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 101, P. 106264 - 106264

Published: Oct. 9, 2024

Language: Английский

Citations

1
Bone improvement in osteoporotic rabbits using CoCrMo implants DOI Creative Commons
Jésica Zuchuat, Adriana S. Manzano,

Valeria Sigot

et al.

Engineered Regeneration, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

The management of bone repair in patients with osteoporosis depends on the clinical situation and extent damage. lesions by inducing new formation is important for maintaining architecture density. Herein, we reported use Cobalt Chromium Molybdenum (CoCrMo) implants osteoporotic rabbits regenerative outcomes vivo. aim was to determine whether placement CoCrMo plates would induce qualitative quantitative differences tissue beneath surrounding implant. We assessed effect alloy animals receiving 4 8 weeks compared results those non-implanted healthy controls. After weeks, minimal histological changes were observed, whereas after a marked osteogenesis evident both apposition substitution bone. In addition, greater number Haversian canals increased canal area decreased intracortical pores observed implanted vs non limb experimental groups. show first time that CrCoMo induces under conditions. beneficial localised cortical areas contact material. Although this may not directly influence OP disease itself, it has direct implications adjacent biomaterial. This potential enhancement could play crucial role improving implant fixation compromised bone, offering biocompatibility stability.

Language: Английский

Citations

0
Harnessing Nature’s Ingenuity to Engineer Butterfly-Wing-Inspired Photoactive Nanofiber Patches for Advanced Postoperative Tumor Treatment DOI
Hao Zheng, Kerong Chen,

Yitong Dun

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122808 - 122808

Published: Sept. 7, 2024

Language: Английский

Citations

0
Polydopamine-Functionalized Calcium-Deficient Hydroxyapatite 3D-printed Scaffold with Sustained Doxorubicin Release for Synergistic Chemo-Photothermal Therapy of Osteosarcoma and Accelerated Bone Regeneration DOI Creative Commons
Lu Wang, Zihan Dai,

Jianqiang Bi

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101253 - 101253

Published: Sept. 25, 2024

Interior bone-tissue regeneration and rapid tumor recurrence post-resection are critical challenges in osteosarcoma other bone cancers. Conventional tissue engineering scaffolds lack inhibitory effects on recurrence. Herein, multifunctional (named DOX/PDA@CDHA) were designed through the spontaneous polymerization of Dopamine (PDA) surface Calcium Deficient Hydroxyapatite (CDHA) scaffolds, followed by situ loading chemotherapeutic drug Doxorubicin (DOX). The PDA coating endowed with significant photothermal properties, while gradual release DOX provided an effective effect. on-demand at sites, triggered dual stimulation (near-infrared (NIR) light acidic pH typical microenvironments), specifically targets cancer cells, thereby mitigating systemic side effects. These unique characteristics facilitated eradication both vitro vivo. Moreover, scaffold's composition, which mimics mineral phase natural is enhanced PDA's biocompatibility, promotes osteogenic angiogenic processes. This facilitates not only but also healthy tissue. Collectively, this study presents a potent candidate for defects induced osteosarcoma.

Language: Английский

Citations

0