Unveiling the Heart’s Hidden Enemy: Dynamic Insights into Polystyrene Nanoplastic-Induced Cardiotoxicity Based on Cardiac Organoid-on-a-Chip

ACS Nano, Journal Year: 2024, Volume and Issue: 18(45), P. 31569 - 31585

Published: Nov. 1, 2024

Exposure to micro- and nanoplastics (MNPs) has been implicated in potential cardiotoxicity. However, vitro models based on cardiomyocyte cell lines lack crucial cardiac characteristics, while interspecies differences animal compromise the reliability of conclusions. In addition, current research predominantly focused single-time point exposures MNPs, neglecting comparative analyses injury across early late stages. Moreover, there remains a large gap understanding susceptibility MNPs under pathological conditions. To address these limitations, this study integrated organoids (COs) organ-on-a-chip (OoC) technology develop organoid-on-a-chip (COoC), which was validated for cardiotoxicity evaluation through multiple dimensions. Based COoC, we conducted dynamic observation damage caused by short- long-term exposure polystyrene (PS-NPs). Oxidative stress, inflammation, disruption calcium ion homeostasis, mitochondrial dysfunction were confirmed as mechanisms PS-NP-induced events stages, fibrosis emerged prominent feature Notably, low-dose exacerbated myocardial infarction symptoms states, despite no significant shown healthy models. conclusion, findings further deepened our cardiotoxic effects introduced promising platform assessing

Language: Английский

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Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 198 - 198

Published: Feb. 18, 2025

Conventionally, root canal treatment is performed when the dental pulp severely damaged or lost due to trauma bacterial endodontic infections. This involves removing compromised infected tissue, disinfecting system, and sealing it with inert, non-degradable materials. However, contemporary has shifted from merely obturating system inert materials guiding tissue regeneration through biological approaches. The ultimate goal of regenerative endodontics restore structural organization functional characteristics akin native pulp, leveraging advancements in engineering biomaterial sciences. Dental commonly employs scaffold-based strategies, utilizing biomaterials as initial platforms for cell growth factor delivery, which subsequently act scaffolds proliferation, differentiation maturation. cells possess an intrinsic capacity self-organization into spheroids can generate their own extracellular matrix, eliminating need external scaffolds. self-assembling property presents a promising alternative scaffold-free engineering, addressing limitations associated biomaterial-based review provides comprehensive overview cell-based, approaches highlighting potential advantages challenges advancing endodontics.

Language: Английский

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Biomimetic Approaches in the Development of Optimised 3D Culture Environments for Drug Discovery in Cardiac Disease

Biomimetics, Journal Year: 2025, Volume and Issue: 10(4), P. 204 - 204

Published: March 26, 2025

Cardiovascular disease remains the leading cause of death worldwide, yet despite massive investment in drug discovery, progress cardiovascular drugs from lab to clinic slow. It is a complex, costly pathway discovery and failure becomes more expensive as progresses along this pathway. The focus has begun shift optimisation vitro culture methodologies, not only because these must be undertaken are earlier on pathway, but also principles 3Rs have become embedded national international legislation regulation. Numerous studies shown myocyte cell behaviour much physiologically relevant 3D compared 2D culture, highlighting advantages using 3D-based models, whether microfluidic or otherwise, for preclinical screening. This review aims provide an overview challenges limitations traditional routes, successes field models discovery. focuses particular role biomimicry can play, around implementation within commercial

Language: Английский

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Tailoring the Therapeutic Potential of Stem Cell Spheroid-Derived Decellularized ECM through Post-Decellularization BDNF Incorporation to Enhance Brain Repair

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123332 - 123332

Published: April 1, 2025

Language: Английский

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Tumor Spheroids, Tumor Organoids, Tumor Explants, and Tumoroids: What Are the Differences between Them?

Biochemistry (Moscow), Journal Year: 2025, Volume and Issue: 90(2), P. 200 - 213

Published: Feb. 1, 2025

Language: Английский

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CRISPR-based genetically modified scaffold-free biomaterials for tissue engineering and regenerative medicine

Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review focuses on CRISPR-based genetically modified extracellular vehicles, cell sheets, aggregates, organoids, and organs, elaborating their applications future directions in tissue engineering regenerative medicine.

Language: Английский

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Recent Advances in Three-Dimensional In Vitro Models for Studies of Liver Fibrosis

Tissue Engineering and Regenerative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Language: Английский

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Research trends of piezoelectric biomaterials in osteochondral tissue engineering

Materials Today Communications, Journal Year: 2024, Volume and Issue: 41, P. 110264 - 110264

Published: Aug. 29, 2024

Language: Английский

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Bioinstructive Liquefied Pockets in Hierarchical Hydrogels and Bioinks

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Abstract This study proposes a novel, versatile, and modular platform for constructing porous heterogeneous microenvironments based on the embedding of liquefied‐based compartments in hydrogel systems. Using bottom‐up approach, microgels carrying necessary cargo components, including cells microparticles, are combined with precursor to fabricate hierarchical structured (HS) system. The HS system possesses three key features that can be fully independently controlled: I) liquefied pockets enabling free cellular mobility; II) surface modified microparticles facilitating 3D microtissue organization inside pockets; III) at larger scale, jammed hydrogel, forming macro‐sized construct. After crosslinking, embedded undergo liquefaction process, structure ensures high diffusion small biomolecules enables move freely within their miniaturized compartmentalized volume. More importantly, this allows creation multimodular controlled macrostructures, while decoupling micro‐ macroenvironments. As proof concept, enhancement functions using by encapsulating human adipose‐derived mesenchymal stem (hASCs) is successfully demonstrated. Finally, potential application as hybrid bioink bioprinting complex structures showcased.

Language: Английский

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Bioengineering strategies for regeneration of skin integrity: A literature review

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 28, P. 153 - 160

Published: Dec. 15, 2024

Language: Английский

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