Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges
ACS Applied Materials & Interfaces,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 24, 2025
The
year
2024
marks
the
50th
anniversary
of
discovery
surface-enhanced
Raman
spectroscopy
(SERS).
Over
recent
years,
SERS
has
experienced
rapid
development
and
became
a
critical
tool
in
biomedicine
with
its
unparalleled
sensitivity
molecular
specificity.
This
review
summarizes
advancements
challenges
substrates,
nanotags,
instrumentation,
spectral
analysis
for
biomedical
applications.
We
highlight
key
developments
colloidal
solid
an
emphasis
on
surface
chemistry,
hotspot
design,
3D
hydrogel
plasmonic
architectures.
Additionally,
we
introduce
innovations
including
those
interior
gaps,
orthogonal
reporters,
near-infrared-II-responsive
properties,
along
biomimetic
coatings.
Emerging
technologies
such
as
optical
tweezers,
nanopores,
wearable
sensors
have
expanded
capabilities
single-cell
single-molecule
analysis.
Advances
analysis,
signal
digitalization,
denoising,
deep
learning
algorithms,
improved
quantification
complex
biological
data.
Finally,
this
discusses
applications
nucleic
acid
detection,
protein
characterization,
metabolite
monitoring,
vivo
spectroscopy,
emphasizing
potential
liquid
biopsy,
metabolic
phenotyping,
extracellular
vesicle
diagnostics.
concludes
perspective
clinical
translation
SERS,
addressing
commercialization
potentials
tissue
sensing
imaging.
Language: Английский
Artificial Intelligence Enabled Biomineralization for Eco‐Friendly Nanomaterial Synthesis: Charting Future Trends
Vaisali Chandrasekar,
No information about this author
Anu Jayanthi Panicker,
No information about this author
Ajay Vikram Singh
No information about this author
et al.
Nano Select,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 30, 2025
ABSTRACT
The
applications
of
nanoparticles
(NPs)
have
shown
tremendous
growth
during
the
last
decade
in
field
biomedicine.
Although
chemical
and
physical
methods
dominate
large‐scale
NP
synthesis,
such
are
also
known
for
their
adverse
impact
on
environment
health.
In
contrast,
use
biological
systems
provides
a
sustainable
alternative
producing
functional
NPs
by
biomineralization
process.
transformative
power
artificial
intelligence
(AI)
has
been
proven
prudent
diagnosis,
drug
development,
therapy,
clinical
decision‐making.
AI
can
be
utilized
tailored
design,
scale‐up
biomedical
applications.
present
review
an
overview
process
its
advantages
over
other
eco‐friendly
synthesis
opportunities.
Specific
emphasis
is
provided
application
cancer
therapy
how
biologically
compatible
improve
management.
Finally,
to
best
our
knowledge,
potential
integrating
comprehensively
analyzed
first
time.
Additionally,
help
surpass
conventionally
synthesized
toxicity
toxicology
material
science
provided.
Language: Английский
3D Tumor-Mimicking Phantom Models for Assessing NIR I/II Nanoparticles in Fluorescence-Guided Surgical Interventions
Asma Harun,
No information about this author
Nathaniel Bendele,
No information about this author
M. Khalil
No information about this author
et al.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 6, 2025
ABSTRACT
Fluorescence
image-guided
surgery
(FIGS)
offers
high
spatial
resolution
and
real-time
feedback
but
is
limited
by
shallow
tissue
penetration
autofluorescence
from
current
clinically
approved
fluorophores.
The
near-infrared
(NIR)
spectrum,
specifically
the
NIR-I
(700-900
nm)
NIR-II
(950-1700
nm),
addresses
these
limitations
with
deeper
improved
signal-to-noise
ratios.
However,
biological
barriers
suboptimal
optical
performance
under
surgical
conditions
have
hindered
clinical
translation
of
NIR-I/II
nanoprobes.
In
vivo
mouse
models
shown
promise,
do
not
replicate
complex
scenarios
encountered
during
real-world
surgeries.
Existing
tissue-mimicking
phantoms
used
to
evaluate
imaging
systems
are
useful
fall
short
when
assessing
nanoprobes
in
environments.
These
often
fail
tumor
microenvironment,
limiting
their
predictive
assessment.
To
overcome
challenges,
we
propose
developing
tumor-mimicking
phantom
(TMPs)
that
integrate
key
features,
such
as
tunable
cell
densities,
-like
nanoparticle
concentrations,
biologically
relevant
factors
(pH,
enzymes),
light
absorption
components
(hemoglobin),
scattering
(intralipid).
TMPs
enable
more
assessments
nanoprobes,
including
profiling,
margin
delineation,
ex
thoracic
on
porcine
lungs.
can
be
further
modulated
closely
match
profiles
tumors.
Additionally,
3D
bioprinting
technology
facilitates
a
high-throughput
platform
for
screening
realistic
conditions.
This
approach
will
identify
high-performing
probes
superior
utility,
bridging
gap
between
preclinical
findings
applications,
ensuring
results
extend
beyond
traditional
studies.
TOC
Language: Английский
3D Tumor-Mimicking Phantom Models for Assessing NIR I/II Nanoparticles in Fluorescence-Guided Surgical Interventions
Asma Harun,
No information about this author
Nathaniel Bendele,
No information about this author
M. Khalil
No information about this author
et al.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 16, 2025
Fluorescence
image-guided
surgery
(FIGS)
offers
high
spatial
resolution
and
real-time
feedback
but
is
limited
by
shallow
tissue
penetration
autofluorescence
from
current
clinically
approved
fluorophores.
The
near-infrared
(NIR)
spectrum,
specifically
the
NIR-I
(700-900
nm)
NIR-II
(950-1700
nm),
addresses
these
limitations
with
deeper
improved
signal-to-noise
ratios.
However,
biological
barriers
suboptimal
optical
performance
under
surgical
conditions
have
hindered
clinical
translation
of
NIR-I/II
nanoprobes.
In
vivo
mouse
models
shown
promise,
do
not
replicate
complex
scenarios
encountered
during
real-world
surgeries.
Existing
tissue-mimicking
phantoms
used
to
evaluate
imaging
systems
are
useful
fall
short
when
assessing
nanoprobes
in
environments.
These
often
fail
tumor
microenvironment,
limiting
their
predictive
assessment.
To
overcome
challenges,
we
propose
developing
tumor-mimicking
phantom
(TMPs)
that
integrate
key
features,
such
as
tunable
cell
densities,
vivo-like
nanoparticle
concentrations,
biologically
relevant
factors
(pH,
enzymes),
light
absorption
components
(hemoglobin),
scattering
(intralipid).
TMPs
enable
more
assessments
nanoprobes,
including
profiling,
margin
delineation,
ex
thoracic
on
porcine
lungs.
can
be
further
modulated
closely
match
profiles
tumors.
Additionally,
3D
bioprinting
technology
facilitates
a
high-throughput
platform
for
screening
realistic
conditions.
This
approach
will
identify
high-performing
probes
superior
utility,
bridging
gap
between
preclinical
findings
applications,
ensuring
results
extend
beyond
traditional
studies.
Language: Английский
Ternary Copolymers Incorporating Tunable Conjugated Donors for Near‐Infrared II Fluorescence Imaging and Photothermal Therapy
Qiang Qi,
No information about this author
Zhongxiu Jiang,
No information about this author
Jianqing Li
No information about this author
et al.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: June 2, 2025
The
near-infrared
second
window
(NIR-II)
offers
excellent
spatial
resolution
and
tissue
penetration,
drawing
attention
to
NIR-II
laser-excited
photothermal
therapy
(PTT)
fluorescence
imaging.
However,
integrating
these
two
applications
poses
challenges
due
safe
laser
power
reagent
dosage
constraints.
To
overcome
issues,
we
proposed
modifying
the
ternary
copolymer
backbone
by
substituting
4H-cyclopenta[2,1-b:3,4-b']dithiophene
(DTC)
with
4,9-dihydro-s-indaceno[1,2-b:5,6-b']dithiophene
(IDT),
enabling
a
transition
from
agent.
Adjusting
molar
ratios
of
donor
acceptor
units
coupling
either
DTC
or
IDT
in
polymer
significantly
affects
their
photophysical
properties.
Among
synthesized
copolymers,
PDTC-253
showed
optimal
performance,
while
PIDT-253
exhibited
high
quantum
yields.
Both
were
used
create
biocompatible
nanoparticles
(NPs).
Under
1064
nm
irradiation,
NPs
effectively
eliminated
4T1
cancer
cells
vitro.
In
vivo
studies
mice
bearing
tumors
that
could
accumulate
at
tumor
sites,
facilitating
treatment
varying
sizes
under
provided
long-term
tracking
through
imaging,
clearly
visualizing
blood
vessels
even
wavelengths
reaching
1500
nm.
This
approach
advances
development
effective
phototheranostic
agents
for
PTT
imaging
window.
Language: Английский
Conjugated Polymer for NIR-II Phototheranostics toward Tumor
Jing Wen Pan,
No information about this author
Wanlu Li,
No information about this author
Mengyao Wang
No information about this author
et al.
Chemistry of Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 9, 2024
Due
to
the
strong
light
trapping
ability,
good
biocompatibility,
and
easily
regulated
molecular
structure,
conjugated
polymers
are
becoming
a
promising
tool
for
phototheranostics
of
tumors,
which
can
achieve
both
precise
diagnosis
efficient
therapeutics.
Of
particular
interest
is
polymer-based
theranostic
agents
applying
second
near-infrared
(NIR-II)
window
(1000–1700
nm),
exhibit
great
potential
clinical
applications
on
account
extremely
long
wavelength.
Herein,
basic
mechanism,
advances
in
recent
6
years,
perspectives
polymer
NIR-II
toward
tumor
discussed
successively.
First,
mechanisms
most
widely
employed
modalities
presented.
In
view
heat
generation
property
under
photoexcitation,
fluorescence
imaging
(FLI)-guided
photothermal
therapy
(PTT)
photoacoustic
(PAI)-guided
PTT.
Then,
related
FLI-guided
PTT
technology,
PAI-guided
multimodel
theranostics
introduced.
Finally,
development
illustrated
practical
application
simultaneously
situ
tumor.
Language: Английский