Ablation of Gap Junction Protein Improves the Efficiency of Nanozyme‐Mediated Catalytic/Starvation/Mild‐Temperature Photothermal Therapy

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(22)

Published: March 25, 2023

Reactive oxygen species (ROS)-mediated tumor catalytic therapy is typically hindered by gap junction proteins that form cell-to-cell channels to remove cytotoxic ROS, thereby protecting cells from oxidative damage. In this work, a multifunctional nanozyme, FePGOGA, designed and prepared Fe(III)-mediated polymerization (FeP), followed glucose oxidase (GOx) GAP19 peptides co-loading through electrostatic π-π interactions. The FePGOGA nanozyme exhibits excellent cascade peroxidase- glutathione-oxidase-like activities efficiently catalyze hydrogen peroxide conversion hydroxyl radicals convert reduced glutathione oxidized disulfide. loaded GOx starves the tumors aggravates stress decomposition, while block hemichannels inducing degradation of Cx43, thus increasing accumulation intracellular decreasing transport glucose. Furthermore, ROS reacts with primary amines heat shock destroy their structure function, enabling photothermal at widely sought-after mild temperature (mildPTT, ≤45 °C). vivo experiments demonstrate significant antitumor effectof on cal27 xenograft under near-infrared light irradiation. This study demonstrates successful ablation overcome resistance ROS-mediated therapy, providing regulator suppress self-preservation during starvation, mildPTT.

Language: Английский

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Continuous Spatiotemporal Therapy of A Full-API Nanodrug via Multi-Step Tandem Endogenous Biosynthesis

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 25, 2023

Abstract Nanomedicine holds great promise to enhance cancer therapy. However, low active pharmaceutical ingredient (API) loading content, unpredictable drug release, and potential toxicity from excipients limit their translational capability. We herein report a full-API nanodrug composed of FDA-approved 5-aminolevulinic acid (ALA), human essential element Fe 3+ , natural bioactive compound curcumin with an ideal API content pH-responsive release profile for continuous spatiotemporal therapy achieved by multi-step tandem endogenous biosynthesis. First, ALA enzymatically converts into photosensitizer protoporphyrin IX (PpIX). Afterward, multiple downstream products including carbon monoxide (CO), 2+ biliverdin (BV), bilirubin (BR) are individually biosynthesized through the PpIX-heme-CO/Fe /BV-BR metabolic pathway, further cooperating released curcumin, ultimately eliciting mitochondria damage, membrane disruption, intracytoplasmic injury. This work not only provides paradigm exploiting diversified metabolites tumor suppression, but also presents safe efficient nanodrug, facilitating practical translation nanodrugs.

Language: Английский

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Stimuli-responsive peptide assemblies: Design, self-assembly, modulation, and biomedical applications

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 181 - 207

Published: Feb. 2, 2024

Peptide molecules have design flexibility, self-assembly ability, high biocompatibility, good biodegradability, and easy functionalization, which promote their applications as versatile biomaterials for tissue engineering biomedicine. In addition, the functionalization of self-assembled peptide nanomaterials with other additive components enhances stimuli-responsive functions, promoting function-specific that induced by both internal external stimulations. this review, we demonstrate recent advance in molecular design, self-assembly, functional tailoring, biomedical peptide-based nanomaterials. The strategies on synthesis single, dual, multiple various dimensions are analyzed, regulation active such metal/metal oxide, DNA/RNA, polysaccharides, photosensitizers, 2D materials, others discussed. designed temperature-, pH-, ion-, light-, enzyme-, ROS-responsive abilities drug delivery, bioimaging, cancer therapy, gene antibacterial, well wound healing dressing presented This comprehensive review provides detailed methodologies advanced techniques from biology, materials science, nanotechnology, will guide inspire level peptides specific functions applications.

Language: Английский

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Dual Glucose/ROS-Sensitive Injectable Adhesive Self-Healing Hydrogel with Photothermal Antibacterial Activity and Modulation of Macrophage Polarization for Infected Diabetic Wound Healing

ACS Materials Letters, Journal Year: 2023, Volume and Issue: 5(12), P. 3142 - 3155

Published: Oct. 27, 2023

Antibacterial, anti-inflammatory, and healing-promoting treatment of chronically infected wounds is not only clinical problems to be solved urgently but also important needs related people's livelihood. However, multifunctional hydrogel dressings with highly efficient antibacterial, wound microenvironment sensitive drug release performance still remain a challenge. In this work, dressing was achieved by introducing mussel-like super adhesion catechol groups glucose/ROS-sensitive dynamic phenylboronester bond cross-linking network, which endowed the excellent injectable, self-healing, tissue properties, performance. Additionally, high-efficiency photothermal synergistic antibacterial TP@Ag NPs were prepared introduced into hydrogel. The glucose/ROS responsive borate in network could realize NPs, thereby promoting M2 polarization achieve an anti-inflammatory effect. And excess reactive oxygen (ROS) scavenged effect group ROS-sensitive Moreover, biocompatibility antioxidant shown hydrogels. Finally, fully fill adhere irregular through injection significantly promote diabetic healing vivo sterilization, ROS scavenging ability, modulation macrophage reduce inflammatory response. Thus, it illustrated superiority as advanced functional for healing.

Language: Английский

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Design of Near Infrared Light‐Powered Copper Phyllosilicate Nanomotors for Cuproptosis‐Based Synergistic Cancer Therapy

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(18)

Published: Jan. 23, 2024

Abstract Cuproptosis, a newly discovered cell death pathway, has shown great potential in cancer treatment. Herein, near‐infrared (NIR) light‐driven nanomotors (CuSiO 3 @Au‐Pd NMs) are designed for cuproptosis‐assisted synergistic therapy with autonomous mobility and improved cellular uptake tumor penetration. Specifically, the released Cu 2+ ions from CuSiO NMs can induce Fenton‐like reaction, leading to generation of hydroxyl radicals (·OH), accompanied by depletion glutathione within MCF‐7 cells. Additionally, also exhibit excellent photothermal effects, which further promote production ·OH, resulting intensified oxidative stress apoptosis. Moreover, enhanced permeation efficiency via movement under self‐thermophoretic forces proved using 2D experiments 3D multicellular spheroids. The resultant intracellular accumulation oligomerization lipoylated proteins, cuproptosis, along mitochondrial dysfunction pathway. More importantly, both vitro vivo show that could penetrate deeply into tumors anticancer efficacy through multimodal therapeutic methods. These findings manifest promising potentials NIR‐powered Cu‐based high maneuverability future smart therapy.

Language: Английский

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