Covalently Attached Slippery Surface Coatings to Reduce Protein Adsorptions on Poly(dimethylsiloxane) Planar Surfaces and 3D Microfluidic Channels DOI
Yue Cao,

Xingchi Chen,

Avi K. Matarasso

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(7), P. 9987 - 9995

Published: Feb. 10, 2023

Silicone elastomers, such as poly(dimethylsiloxane) (PDMS), have a broad range of applications in basic biomedical research and clinical medicine, ranging from the preparation microfluidic devices for organs-on-chips ventriculoperitoneal shunts treatment hydrocephalus to implantable neural probes neuropharmacology. Despite importance, protein adsorptions on silicone elastomers these application environments represent significant challenge. Surface coatings with slippery lubricants, inspired by Nepenthes pitcher plants, recently received much attention reducing adsorptions. Nevertheless, depletion physically infused lubricants limits their applications. In this study, we report covalently attached surface coating reduce PDMS surfaces. As demonstrations, show that adsorption serum proteins, human fibrinogen albumin, can be significantly reduced both planar surfaces 3D channels. The relies acid-catalyzed polycondensation reaction dimethyldimethoxysilane, which utilizes low-cost scalable dip-coating method. Furthermore, cell metabolic activity viability studies demonstrate biocompatibility coating. These results suggest potential medical devices, organs-on-chips, many others.

Language: Английский

Biomedical Applications of Microfluidic Devices: A Review DOI Creative Commons
Ghazaleh Gharib, İsmail Bütün, Zülâl Muganlı

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 1023 - 1023

Published: Nov. 16, 2022

Both passive and active microfluidic chips are used in many biomedical chemical applications to support fluid mixing, particle manipulations, signal detection. Passive devices geometry-dependent, their uses rather limited. Active include sensors or detectors that transduce chemical, biological, physical changes into electrical optical signals. Also, they transduction detect biological applications, highly versatile tools for disease diagnosis organ modeling. This review provides a comprehensive overview of the significant advances have been made development microfluidics devices. We will discuss function as micromixers sorters cells substances (e.g., microfiltration, flow displacement, trapping). Microfluidic fabricated using range techniques, including molding, etching, three-dimensional printing, nanofabrication. Their broad utility lies detection diagnostic biomarkers organ-on-chip approaches permit modeling cancer, well neurological, cardiovascular, hepatic, pulmonary diseases. Biosensor allow point-of-care testing, assays based on enzymes, nanozymes, antibodies, nucleic acids (DNA RNA). An anticipated field includes optimization techniques fabrication biocompatible materials. These developments increase versatility, reduce costs, accelerate time technology.

Language: Английский

Citations

105
Artificial Intelligence in Regenerative Medicine: Applications and Implications DOI Creative Commons
Hamed Nosrati, Masoud Nosrati

Biomimetics, Journal Year: 2023, Volume and Issue: 8(5), P. 442 - 442

Published: Sept. 20, 2023

The field of regenerative medicine is constantly advancing and aims to repair, regenerate, or substitute impaired unhealthy tissues organs using cutting-edge approaches such as stem cell-based therapies, gene therapy, tissue engineering. Nevertheless, incorporating artificial intelligence (AI) technologies has opened new doors for research in this field. AI refers the ability machines perform tasks that typically require human ways learning patterns data applying without being explicitly programmed. potential improve accelerate various aspects development, particularly, although not exclusively, when complex are involved. This review paper provides an overview context medicine, discusses its applications with a focus on personalized highlights challenges opportunities

Language: Английский

Citations

62
Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications DOI Creative Commons
Sungjin Min, Suran Kim, Woo‐Sup Sim

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 22, 2024

Abstract Engineered human cardiac tissues have been utilized for various biomedical applications, including drug testing, disease modeling, and regenerative medicine. However, the applications of derived from pluripotent stem cells are often limited due to their immaturity lack functionality. Therefore, in this study, we establish a perfusable culture system based on vivo-like heart microenvironments improve tissue fabrication. The integrated platform microfluidic chip three-dimensional extracellular matrix enhances development structural functional maturation. These comprised cardiovascular lineage cells, cardiomyocytes fibroblasts induced as well vascular endothelial cells. resultant macroscale exhibit improved efficacy testing (small molecules with levels arrhythmia risk), modeling (Long QT Syndrome fibrosis), therapy (myocardial infarction treatment). our can serve highly effective tissue-engineering provide versatile applications.

Language: Английский

Citations

30
Bioinspired Superwetting Open Microfluidics: From Concepts, Phenomena to Applications DOI
Yifan Si, Chuxin Li, Jinlian Hu

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(32)

Published: April 25, 2023

Abstract Microfluidics and bioinspired superwetting materials, as two crucial branches of scientific research, are entering their golden age development. As an emerging interdisciplinary subject these fields, open microfluidics is triggering technological revolutions in many disciplines, including rapid medical diagnosis, biochemical analysis, liquid manipulation, 3D printing, etc. However, this new research area has yet to attract extensive attention. So, a timely review necessary organize the development process, summarize current achievements, discuss challenges or chances for ongoing trend. In review, evolution from closed combed first. Then, three typical systems introduced emphatically. Based on this, divided into different categories according bionic objects focus study. Taking natural phenomena entry point, underlying mechanism application systematically discussed summarized. Several applications also mentioned. Finally, some views major problems, existing challenges, developing trends briefly put forward field guide future research.

Language: Английский

Citations

38
Musculoskeletal Organs‐on‐Chips: An Emerging Platform for Studying the Nanotechnology–Biology Interface DOI Creative Commons
Yuwen Wang, Patrick Shu‐Hang Yung, Gang Lü

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: March 16, 2024

Nanotechnology-based approaches are promising for the treatment of musculoskeletal (MSK) disorders, which present significant clinical burdens and challenges, but their translation requires a deep understanding complex interplay between nanotechnology MSK biology. Organ-on-a-chip (OoC) systems have emerged as an innovative versatile microphysiological platform to replicate dynamics tissue microenvironment studying nanotechnology-biology interactions. This review first covers recent advances applications OoCs ability mimic biophysical biochemical stimuli encountered by tissues. Next, integrating into OoCs, cellular responses behaviors may be investigated precisely controlling manipulating nanoscale environment. Analysis disease mechanisms, particularly bone, joint, muscle degeneration, drug screening development personalized medicine greatly facilitated using OoCs. Finally, future challenges directions outlined field, including advanced sensing technologies, integration immune-active components, enhancement biomimetic functionality. By highlighting emerging this aims advance intricate nanotechnology-MSK biology interface its significance in management, therapeutic interventional strategies.

Language: Английский

Citations

14
Hierarchical Design of Tissue‐Mimetic Fibrillar Hydrogel Scaffolds DOI Creative Commons
Alberto Pardo, Manuel Gómez‐Florit, Matthew D. Davidson

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(16)

Published: Feb. 24, 2024

Abstract Most tissues of the human body present hierarchical fibrillar extracellular matrices (ECMs) that have a strong influence over their physicochemical properties and biological behavior. Of great interest is introduction this structure to hydrogels, particularly due water‐rich composition, cytocompatibility, tunable class biomaterials. Here, main bottom‐up fabrication strategies for design production biomimetic hydrogels most representative applications in fields tissue engineering regenerative medicine are reviewed. For example, controlled assembly/arrangement peptides, polymeric micelles, cellulose nanoparticles (NPs), magnetically responsive nanostructures, among others, into discussed, as well potential use fibrillar‐like (e.g., those from NPs) with key biofunctionalities such electrical conductivity or remote stimulation. Finally, major remaining barriers clinical translation future directions research field discussed.

Language: Английский

Citations

12
Bioprinting of self-healing materials and nanostructures for biomedical applications: Recent advances and progresses on fabrication and characterization techniques DOI
Babak Mikaeeli Kangarshahi, Seyed Morteza Naghib, Gelareh Mikaeeli Kangarshahi

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 38, P. e00335 - e00335

Published: Feb. 1, 2024

Language: Английский

Citations

11
Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease DOI Creative Commons
Alexandra Wheeler, Verena Stoeger, Róisı́n M. Owens

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1266 - 1292

Published: Jan. 1, 2024

Further development of lab-on-chip platforms is required to create an environment capable hosting more complex microbiota and immune cells.

Language: Английский

Citations

9
Sustainable biofabrication: from bioprinting to AI-driven predictive methods DOI Creative Commons
Miriam Filippi,

Manuel Mekkattu,

Robert K. Katzschmann

et al.

Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Language: Английский

Citations

9
Pickering emulsions stabilized by soybean protein–based nanoparticles: A review of formulation, characterization, and food‐grade applications DOI
Zhen Yang,

Yuanyang Song,

Hui Chen

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: March 1, 2025

Abstract Pickering emulsions (PEs) have attracted considerable interest as platforms for encapsulating and controlling the release of bioactive compounds. Recent studies emphasize potential soybean protein nanoparticles to improve PE‐based carriers, enhancing stability bioavailability these compounds through unique self‐assembly behaviors. This review analyzes recent advancements in use nanoparticle‐stabilized PEs carriers Various fabrication techniques, including physical, chemical, biological methods, are explored. The effectiveness nanoparticles, both individually combination with polysaccharides or polyphenols, is evaluated, highlighting their roles stabilizing functionality. Findings indicate that effective stabilizers a wide range PE structures, oil‐in‐water, water‐in‐oil, high internal phase PEs, emulgels. Fabrication properties particles, processing parameters, formulations significantly influence interfacial behavior, structure, functionality PEs. Additionally, innovative applications future developments protein–based discussed, emphasizing plant‐based substitutes advanced materials. Despite extensive discussions on various food forms, research into techno‐functional flavor mechanisms remains limited.

Language: Английский

Citations

1